ABSTRACT
The purpose of this study was to investigate the effect of low level laser therapy (LLLT) on male Wistar rat trachea hyperreactivity (RTHR), bronchoalveolar lavage (BAL) and lung neutrophils influx after Gram-negative bacterial lipopolyssacharide (LPS) intravenous injection. The RTHR, BAL and lung neutrophils influx were measured over different intervals of time (90 min, 6 h, 24 h and 48 h). The energy density (ED) that produced an anti-inflammatory effect was 2.5 J/cm(2), reducing the maximal contractile response and the sensibility of trachea rings to methacholine after LPS. The same ED produced an anti-inflammatory effect on BAL and lung neutrophils influx. The Celecoxib COX-2 inhibitor reduced RTHR and the number of cells in BAL and lung neutrophils influx of rats treated with LPS. Celecoxib and LLLT reduced the PGE(2) and TXA(2) levels in the BAL of LPS-treated rats. Our results demonstrate that LLLT produced anti-inflammatory effects on RTHR, BAL and lung neutrophils influx in association with inhibition of COX-2-derived metabolites.
Subject(s)
Bronchial Hyperreactivity/radiotherapy , Low-Level Light Therapy/methods , Pneumonia/radiotherapy , Pyrazoles/pharmacology , Sulfonamides/pharmacology , Animals , Bronchoalveolar Lavage Fluid/cytology , Celecoxib , Chemotaxis, Leukocyte , Dinoprostone/metabolism , Disease Models, Animal , Inflammation Mediators/analysis , Lipopolysaccharides , Male , Neutrophils/cytology , Probability , Random Allocation , Rats , Reference Values , Sensitivity and Specificity , Trachea/physiopathologyABSTRACT
O objetivo do presente estudo foi investigar o efeito do laser de baixa potência (LLLT) na hiper-reatividade da traquéia de ratos Wistar macho(RTHR) depois da administração de lipopolissacarídeo (LPS)