ABSTRACT
Background: Adhesive monomers are not able to fully encapsulate collagen fibrils in hybrid layer leaving them vulnerable to time-dependent hydrolytic degradation. The current in vitro study was designed for investigation of the remineralization of the resin-dentin hybrid layer using biomimetic analogs by nanoleakage investigation. Material and Methods: Firstly, occlusal enamel of thirty human molars was removed exposing flat surface of dentin, then randomly divided into three main groups according to the different remineralizing protocols (n=10) (R): control group (R0), STMP group (R1), and biomimetic remineralizing group (R2). The dentin surface of the STMP and biomimetic remineralizing groups (R1 and R2) was treated with STMP solution, followed by self-etch adhesive application on dentin surface of all groups, and restored with double 2-mm thick layers of resin composite. Each tooth was sectioned perpendicularly to the resin-dentin interface for producing 1-mm thick slaps. Each group was subdivided into four subgroups according to the incubation time to 24 hours, one month, three months, and 4 months. Retrieved slabs were prepared for nanoleakage for evaluation of metallic silver particles distribution percentage at the resin-dentin interface using digital image analysis software. Results: There was a statistically significant increase in nanoleakage over time in all three groups. However, the third group showed the least increase in metallic silver uptake over time. Conclusions: Hybrid layer could be remineralized by using dual-biomimetic analogs (PAA and STMP). Key words:Hybrid layer, remineralization, nanoleakage, polyacrylic acid, sodium trimetaphosphate.