Immune and microbiological disorders in women with infectious and inflammatory pathology of the cervix.

Penetration into the female genital tract of infectious agents is associated with the cause of the onset of cervicitis. In most cases, these are sexually transmitted diseases (STDs). A high level of viral and bacterial load in the study of clinical material from women with infectious and inflammatory pathology (IVP) of the cervix affects the activity of innate immunity reactions. The total number of examined patients was 76 patients of reproductive age, of which 36 women with cervical IVP and 40 women made up the comparison group. The imbalance of microbiocenosis can be accompanied by immune and microbiological disorders. The aim of the study was to study immune and microbiological disorders in women with infectious and inflammatory pathology of the cervix. To achieve the goal, the following tasks were set: to identify the relationship between the expression level of TLR2 and TLR4 genes in the epithelial cells of the cervical canal of women in which UPM is determined. To determine the species spectrum and etiological significance of the microbiota of bacterial biofilm of the cervical mucosa in the pathogenesis of cervicitis in women of reproductive age. It has been established that the determination of indicators of innate immunity, such as interferons, in blood serum and the expression of TLR2 and TLR4 receptors, with the study of their balance, taking into account the pro-inflammatory and anti-inflammatory properties in IVP of the cervix, has an important prognostic value. An increase in the level of the immune response in the form of hyperstimulation of antigens of IVP pathogens leads to the chronization of the inflammatory process in the urogenital tract, to scarring of tissues, which can play an important role in the development of infertility and termination of pregnancy in women of reproductive age.

[THE SPECTRUM AND ANTIBIOTIC RESISTANCE OF AGENTS OF NOSOCOMIAL INFECTION OF OPERATION WOUNDS AND ORGANS OF URINARY EXCRETION SYSTEM IN SURGERY PATIENTS].

In risk group of development of nosocomial infections related to medical care provision, prevailed weakened patients after various surgical interferences and cauterization. The nosocomial infections related to medical care provision develop up to 20% of patients of surgical profile (infections of soft tissues - 9.5%, intra-abdominal infections - 22% and infections of urinary tracts - 4.8%). The Gram-negative bacilli Enerobacteriaceae and Gram-negative nonfermenters.

[Antibiotic resistance of P. aeruginosa strains isolated from intensive care unit patients at the Makhachkala Traumatology Center].

Nosocomial infection is most commonly registered in the traumatology and surgery units, primarily in the intensive care unit (ICU) that is considered to be an increased risk unit owing to a large number of performed manipulations and the extensive empirical use of antibiotics. In ICU patients with nosocomial pneumonias at the Traumatology Center, nonfermentative gram-negative bacteria were isolated more frequently (51%), including P. aeruginosa (35.1%), from both patients' pathological material and environmental objects. All the cultures had typical properties; the majority of isolates belonged to serovar 02. P. aeruginosa strains were found to be polyresistant to test antibiotics.

[Microbiological study of sanitary feature of Perinatal Center of Makhachkala City].

AIM: Evaluation of bacterial contamination of six hospital environment of Perinatal Center of Makhachkala as part of epidemiologic surveillance for nosocomial infections. MATERIALS AND METHODS: One hundred twenty-eight air samples from different hospital units and 344 swabs from hospital equipment, instruments, and inventory were tested. Dry nutrient media manufactured by Scientific Manufacturing Organization "Pitatelnye Sredy" were used for isolation and identification of microorganisms. Species of microorganisms was determined on the basis of complex of tinctorial, morphological, biochemical, and serologic tests. RESULTS: Significant species diversity of opportunistic microorganisms was established. Cultures of Staphylococcus epidermidis (46; 18.5%) and Staphylococcus saprophyticus (44; 17.7%) were significantly more frequently isolated from swabs from environment. CONCLUSION: Microbiological monitoring of sanitary conditions of perinatal center assists sanitary-epidemiologic control for circulation of microorganisms--potential agents of nosocomial infections.