1.
Diagn Microbiol Infect Dis
; 78(4): 360-2, 2014 Apr.
Article
in English
| MEDLINE
| ID: mdl-24582319
ABSTRACT
The Directigen EZ Flu A+B rapid influenza diagnostic test, as compared to real-time reverse transcriptase polymerase chain reaction, demonstrated suboptimal performance to detect pandemic influenza A/H1N1 2009. Age- and viral load-stratified test sensitivity ranged from 33.3 to 84.6% and 0 to 100%, respectively.
Subject(s)
Chromatography, Affinity/methods , Diagnostic Tests, Routine/methods , Influenza A Virus, H1N1 Subtype/isolation & purification , Influenza, Human/diagnosis , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Female , Humans , Infant , Influenza, Human/virology , Male , Middle Aged , Sensitivity and Specificity , Young Adult
2.
J Clin Microbiol
; 52(3): 957-9, 2014 Mar.
Article
in English
| MEDLINE
| ID: mdl-24352998
ABSTRACT
We characterize a novel probe binding-site polymorphism detectable solely by melt curve analysis using the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay. The frequencies of this novel (47°C) and previously described intermediate (60 to 62°C) melt curves were 0.016% and 4.9%, respectively.
