ABSTRACT
Cystic echinococcosis (CE) is a parasitic zoonosis caused by the larval stage of the tapeworm Echinococcus granulosus. Detection of the adult stage in the canine definitive host is essential for estimating infection rates, surveillance and monitoring of CE control programs. This study sought to develop and validate a coproantigen sandwich enzyme-linked immunosorbent assay (copro-ELISA), based on antibodies against E. granulosus-soluble membrane antigens (EGMA), that is capable of distinguishing infected and noninfected dogs. Anti-E. granulosus polyclonal immunoglobulin G antibodies were obtained from rabbit antiserum against EGMA. Optimization of the test was performed with 51 positive and 56 negative stool samples of canine echinococcosis. Specificity, sensitivity, cross-reactivity, intra- and inter-assay precision, and over time detection were evaluated. According to the receiver operating characteristic analysis, the diagnostic sensitivity and specificity were 96.1% (CI: 85.9-99.6) and 98.2% (CI: 89.5-100), respectively. Negative and positive predictive values were 96.5% (CI: 91.7-100) and 98% (CI: 94.1-100), respectively. No cross-reactivity with Taenia hydatigena, Dipylidium caninum, or Toxocara canis was observed. Intra- and inter-assay repeatability showed values of less than 15% of the variation coefficient. The over time detection was from 20 to 27 days postinfection with E. granulosus. The copro-ELISA based on EGMA detection offers a simplified in-house development of diagnostic testing. This assay showed high specificity and sensitivity and had no cross-reactivity with other parasites. Further studies and development of this test in a kit format may be useful for the detection of active infection in dogs living in CE endemic regions.
Subject(s)
Antibodies, Helminth/chemistry , Antigens, Helminth/analysis , Dog Diseases/diagnosis , Echinococcosis/diagnosis , Echinococcosis/veterinary , Echinococcus granulosus/immunology , Enzyme-Linked Immunosorbent Assay/standards , Animals , Dog Diseases/epidemiology , Dog Diseases/immunology , Dog Diseases/parasitology , Dogs , Echinococcosis/epidemiology , Echinococcosis/immunology , Echinococcus granulosus/isolation & purification , Feces/parasitology , Humans , Larva/immunology , Observer Variation , Peru/epidemiology , Rabbits , Sensitivity and SpecificityABSTRACT
Objetivo: Determinar la prevalencia de enteropárasitos con potencial zoonótico en pacientes pediátricos que fueron atendidos en el Hospital de Andahuaylas durante los años 2008 a 2010. Metodología: A partir de 460 historias clínicas de pacientes pediátricos se recogieron datos de la edad, sexo, procedencia y resultados del examen coproparasitológico. Resultados y conclusiones: Se encontró que el 47,6% (219/460) de niños tenía algún parásito con potencial zoonótico. Los porcentajes individuales de los parásitos con potencial zoonótico hallados fueron: Giardia intestinalis 25,9% (119/460), Entamoeba coli 18,5% (85/460), Blastocystis sp. 14,4% (66/460), Hymenolepis nana 2,6% (12/460), Entamoeba hystolitica 0,4% (2/460), Taenia sp. 0,4% (2/460) y Strongyloides sp. 0,2% (1/460). Se muestran adicionales análisis porcentuales.
Aim: To determine the prevalence of intestinal parasites with zoonotic potential in pediatric patients who attend the Andahuaylas Hospital during 2008 to 2010. Methodology: By using 460 pediatric patients medical records specific data of sex, origin and coproparasitological examination report were collected. Results and conclusions: We found 47,6% (219/460) of children with parasites with zoonotic potential. Giardia intestinalis had the highest prevalence with 25,9% (119/460), Entamoeba coli 18,5% (85/460), Blastocystis sp. 14,4% (66/460), Hymenolepis nana 2,6% (12/460), Entamoeba hystolitica 0,4% (2/460), Taenia sp. 0,4% (2/460) and Strongyloides sp. 0,2%.We show further percental analysis.
