1.
Vaccine
; 18(22): 2331-6, 2000 May 08.
Article
in English
| MEDLINE
| ID: mdl-10738088
ABSTRACT
DNA vaccines which work in winter are needed for fish. At 18 weeks after intramuscular injection of a plasmid containing lacZ goldfish at 15 degrees C had five-fold more antibody to betagalactosidase than those at 25 degrees C or those at =9 degrees C. Expression persisted for 1 week at 25 degrees C and for 4 weeks at 15 degrees C. Expression was highest after 18 weeks at =9 degrees C and was correlated to antibody titre in this group. Goldfish on low or high rations (0.33 or 3% body weight daily) made the same amount of antibody whilst losing 20% or gaining 50% of their body-weight over 2 months at 22 degrees C.