Characterization of Ingredients Incorporated in the Traditional Mixed-Salad of the Capuchin Monks.

Recipes on the composition of the "salad of the monks" (Capuchin monks) have been reported in Italy since the 17th century. Different wild edible plants were highly regarded as an important ingredient of this mixed salad. Among these, some species played a key role for both their taste and nutritional properties: Plantago coronopus L. (PC), Rumex acetosa L., Cichorium intybus L., and Artemisia dracunculus L. In the present study, the micromorphological and phytochemical features as well as the antioxidant and anti-inflammatory properties of extracts of these fresh and blanched leaves, were investigated. The extracts obtained by blanched leaves, according to the traditionally used cooking method, showed the highest content of bioactive compounds (total phenols 1202.31-10,751.88 mg GAE/100 g DW; flavonoids 2921.38-61,141.83 mg QE/100 g DW; flavanols 17.47-685.52 mg CE/100 g DW; proanthocyanidins 2.83-16.33 mg CyE/100 g DW; total chlorophyll 0.84-1.09 mg/g FW; carbohydrates 0.14-1.92 g/100 g FW) and possess the most marked antioxidant (IC50 0.30-425.20 µg/mL) and anti-inflammatory activity (IC50 240.20-970.02 µg/mL). Considering this, our results indicate that increased consumption of the investigated plants, in particular of PC, raw or cooked briefly, could provide a healthy food source in the modern diet by the recovery and enhancement of ancient ingredients.

Comparative and Functional Screening of Three Species Traditionally used as Antidepressants: Valeriana officinalis L., Valeriana jatamansi Jones ex Roxb. and Nardostachys jatamansi (D.Don) DC.

The essential oils (EOs) of three Caprifoliaceae species, the Eurasiatic Valeriana officinalis (Vo), the Himalayan Valeriana jatamansi (Vj) and Nardostachys jatamansi (Nj), are traditionally used to treat neurological disorders. Roots/rhizomes micromorphology, DNA barcoding and EOs phytochemical characterization were carried out, while biological effects on the nervous system were assessed by acetylcholinesterase (AChE) inhibitory activity and microelectrode arrays (MEA). Nj showed the highest inhibitory activity on AChE (IC50 67.15 µg/mL) followed by Vo (IC50 127.30 µg/mL) and Vj (IC50 246.84 µg/mL). MEA analyses on rat cortical neurons, carried out by recording mean firing rate (MFR) and mean bursting rate (MBR), revealed stronger inhibition by Nj (IC50 18.8 and 11.1 µg/mL) and Vo (16.5 and 22.5 µg/mL), compared with Vj (68.5 and 89.3 µg/mL). These results could be related to different EO compositions, since sesquiterpenes and monoterpenes significantly contribute to the observed effects, but the presence of oxygenated compounds such as aldehydes and ketones is a discriminating factor in determining the order of potency. Our multidisciplinary approach represents an important tool to avoid the adulteration of herbal drugs and permits the evaluation of the effectiveness of EOs that could be used for a wide range of therapeutic applications.

Traditional Uses of Medicinal Plants by Ethnic People in the Kavrepalanchok District, Central Nepal.

In rural areas of Nepal, where it is difficult to get access to Government health care facilities, people depend on medicinal plants and local healers for health problems. This study concerns an ethnobotanical survey of the Kavrepalanchok District, reporting some unusual uses of medicinal plants and original recipes. A total of 32 informants were interviewed, 24 of them being key informants. Ethnobotanical uses concerned 116 taxa, of which 101 were medicinal plants, with the most representative species belonging to Asteraceae, Fabaceae, Lamiaceae, and Zingiberaceae. Ethnobotanical indexes were used to evaluate the ethnopharmacological importance of each plant species and the degree of agreement among the informants' knowledge. Informant consensus factor (Fic) showed that the fever category had the greatest agreement. Highest fidelity level (FL) values were found for Calotropis gigantea used for dermatological diseases, Drymaria cordata for fever, Mangifera indica and Wrightia arborea for gastrointestinal disorders. Data document the richness of the local flora and the traditional knowledge on medicinal plant species used by ethnic communities in rural areas. The active involvement of local populations in the conservation and management of medicinal plant species will encourage future projects for the sustainable development of the biological and cultural diversity of these rural areas of Nepal.