Oxidation of winery wastewater by sulphate radicals: catalytic and solar photocatalytic activations.

The treatment of winery effluents through sulphate radical-based advanced oxidation processes (SR-AOPs) driven by solar radiation is reported in this study. Photolytic and catalytic activations of peroxymonosulphate (PMS) and persulphate (KPS and SPS) at different pH values (4.5 and 7) were studied in the degradation of organic matter. Portugal is one of the largest wine producers in Europe. The wine making activities generate huge volume of effluents characterized by a variable volume and organic load, being their seasonal nature one of the most important drawbacks. Recently, SR-AOPs are gradually attracting attention as in situ chemical oxidation technologies, instead of hydroxyl radical AOPs (HR-AOPs). The studied concentrations are suitable to obtain notable values of organic matter degradation, with TOC removal around 50%. In general terms, no notable differences were observed between treatments at pH values 4.5 and 7. Photolytic activation of SPS with solar radiation treatments obtained the highest efficiency (28 and 40% of TOC removal with 1 and 50 mM, respectively, at pH 4.5) in comparison to KPS and PMS. The addition of a transition metal as catalyst, such as Fe(II) or Co(II), increased considerably the TOC removal efficiency higher than 50%, but not in all cases. For instance, the combination KPS or PMS with Co(II) at pH 4.5 did not allow to obtain better results than photolytic activation of these persulphate salts. In summary, the use of SR-AOPs could be a serious alternative as tertiary treatment for winery wastewaters.

Treatment of crystallized-fruit wastewater by UV-A LED photo-Fenton and coagulation-flocculation.

This work reports the treatment of crystallized-fruit effluents, characterized by a very low biodegradability (BOD5/COD <0.19), through the application of a UV-A LED photo-Fenton process. Firstly, a Box-Behnken design of Response Surface Methodology was applied to achieve the optimal conditions for the UV-A LED photo-Fenton process, trying to maximize the efficiency by saving chemicals and time. Under the optimal conditions ([H2O2] = 5459 mg/L; [Fe(3+)] = 286 mg/L; time >180 min), a COD removal of 45, 64 and 74% was achieved after 360 min, using an irradiance of 23, 70 and 85 W/m(2) respectively. Then a combination of UV-A LED photo-Fenton with coagulation-flocculation-decantation attained a higher COD removal (80%), as well as almost total removal of turbidity (99%) and total suspended solids (95%). Subsequent biodegradability of treated effluents increased, allowing the application of a biological treatment step after the photochemical/CFD with 85 W/m(2).

Combined treatment of olive mill wastewater by Fenton's reagent and anaerobic biological process.

This work presents the application of Fenton's reagent process combined with anaerobic digestion to treat an olive mill wastewater (OMW). Firstly, OMW was pre-treated by chemical oxidation in a batch reactor with Fenton's reagent, using a fixed H2O2/COD ratio of 0.20, pH = 3.5 and a H2O2/Fe(2+) molar ratio of 15:1. This advanced oxidation treatment allowed reaching reductions of 17.6 and 82.5% of chemical oxygen demand (COD) and total polyphenols (TP), respectively. Secondly, OMW treatment by anaerobic digestion was performed using previously adapted microorganisms immobilized in Sepiolite. These biological tests were carried out varying the substrate concentration supplied to the reactor and COD conversions from 52 to 74% were obtained. Afterwards, Fenton's reagent followed by anaerobic digestion was applied to OMW treatment. This combined process presented a significant improvement on organic load removal, reaching COD degradations from 64 to 88%. Beyond the pollutant load removal, it was also monitored the yield of methane generated throughout anaerobic experiments. The methane produced ranged from 281 cm(3) to 322 cm(3) of CH4/g COD removed. Additionally, a methane generation kinetic study was performed using the Monod Model. The application of this model allowed observing a kinetic constant increase of the combined process (kFN = 0.036 h(-1)) when compared to the single anaerobic process (kF = 0.017 h(-1)).

Mature landfill leachate treatment by coagulation/flocculation combined with Fenton and solar photo-Fenton processes.

This work reports the treatment of a mature landfill leachate through the application of chemical-based treatment processes in order to achieve the discharge legal limits into natural water courses. Firstly, the effect of coagulation/flocculation with different chemicals was studied, evaluating the role of different initial pH and chemicals concentration. Afterwards, the efficiency of two different advanced oxidation processes for leachate remediation was assessed. Fenton and solar photo-Fenton processes were applied alone and in combination with a coagulation/flocculation pre-treatment. This physicochemical conditioning step, with 2 g L(-1) of FeCl3 · 6H2O at pH 5, allowed removing 63% of COD, 80% of turbidity and 74% of total polyphenols. Combining the coagulation/flocculation pre-treatment with Fenton reagent, it was possible to reach 89% of COD removal in 96 h. Moreover, coagulation/flocculation combined with solar photo-Fenton revealed higher DOC (75%) reductions than single solar photo-Fenton (54%). In the combined treatment (coagulation/flocculation and solar photo-Fenton), it was reached a DOC reduction of 50% after the chemical oxidation, with 110 kJ L(-1) of accumulated UV energy and a H2O2 consumption of 116 mM. Toxicity and biodegradability assays were performed to evaluate possible variations along the oxidation processes. After the combined treatment, the leachate under study presented non-toxicity but biodegradability increased.

Arthroscopic tibiotalocalcaneal arthrodesis with locked retrograde compression nail.

Tibiotalocalcaneal (TTC) arthrodesis is a demanding procedure. Several techniques have been described for successful fusion, including the use of plates, screws, intramedullary nails, and external fixators. Arthroscopic TTC fusion with intramedullary nailing has been recently described as an alternative method to traditional open procedures. The surgical technique and clinical and radiographic outcomes of 2 patients who had undergone arthroscopic TTC arthrodesis with intramedullary nailing are presented. The indication for surgery was symptomatic tibiotalar and subtalar post-traumatic arthritis in 1 patient and distal tibia and fibula nonunion in the other. In both cases, fusion was obtained at approximately 8 weeks postoperatively. At the final follow-up visit at 2 years postoperatively, their American Orthopaedic Foot and Ankle Society score had improved from 31 to 85 points and from 16 to 71 points, respectively. No intra- or postoperative complications developed. Arthroscopic TTC arthrodesis can be an alternative to traditional open procedures, especially in patients with soft tissue concerns or several previous surgeries. Experience with ankle arthroscopy procedures is mandatory to obtain satisfactory results and minimize the risk of complications.

Treatment of concentrated fruit juice wastewater by the combination of biological and chemical processes.

Concentrated fruit juice industries use a wide volume of water for washing and fruit processing, generating a large volume of wastewater. This work studied the combination of an aerobic biological process with a chemical coagulation/flocculation step to treat a high concentrated fruit juice wastewater. This wastewater presents a good biodegradability (BOD(5)/COD = 0.66) allowing a chemical oxygen demand (COD) removal above 90% in most reactors. The best results in aerobic biological treatment were obtained in reactors initially loaded with 2 g VSS L(-1) of biomass concentration and 20 g COD L(-1) of organic matter concentration. Three different kinetic models were evaluated (Monod, Haldane and Contois). The Haldane-inhibition model was the one that best fitted the COD biodegradation. AQUASIM software allowed calculate the following kinetic constants ranges for aerobic biodegradation: K (s): 6-20 g COD L(-1); v (max): 2.0-5.1 g COD g(-1) VSS day(-1) and K (i) values: 0.10-0.50 g COD L(-1). These constants corresponds to maximum removal rates (v*) between 0.11 and 0.26 g COD g(-1) VSS day(-1) for substrate concentrations (S*) from 0.77 to 3.16 g COD L(-1). A tertiary coagulation/flocculation process improved the efficiency of the biological pre-treatment. Ferric chloride was selected as best compromise to treat this wastewater. Optimal conditions were 0.44 g L(-1) of coagulant at pH = 5.5, achieving 94.4% and 99.6% on turbidity and COD removal, respectively.

Tertiary treatment of pulp mill wastewater by solar photo-Fenton.

This work reports on pulp mill wastewater (PMW) tertiary treatment by Fenton (Fe(2+)/H(2)O(2)) and solar photo-Fenton (Fe(2+)/H(2)O(2)/UV) processes in a pilot plant based on compound parabolic collectors (CPCs). Solar photo-Fenton reaction is much more efficient than the respective dark reaction under identical experimental conditions. It leads to DOC mineralisation, COD and total polyphenols (TP) removal higher than 90%. The solar photo-Fenton experiment with 5mg Fe L(-1) reaches 90% of DOC mineralisation with 31kJ L(-1) of UV energy and 50mM of H(2)O(2). The initial non-biodegradability of PMW, as shown by respirometry assays and BOD(5)/COD ratio, can be changed after a solar photo-Fenton treatment. Experiments with 20 and 50mg Fe L(-1) revealed that solar photo-Fenton can reach the same DOC degradation (90%), however, consuming less H(2)O(2) and time. Diluting the initial organic load to 50% also diminishes the dosage of H(2)O(2) and the necessary reaction time to achieve high DOC removals. Accordingly, solar photo-Fenton can be considered an alternative or complementary process to improve the performance of a biologic treatment and, subsequently, achieve legal limits on discharge into natural waters.

Morphogenesis and aggressiveness of cervix carcinoma.

The regular nutritional intake of an expectant mother clearly affects the weight development of the fetus. Assuming the growth of the fetus follows a deterministic growth law, like a logistic equation, albeit dependent on the nutritional intake, the ideal solution is usually determined by the birth-weight being pre-assigned, for example, as a percentage of the mother's average weight. This problem can then be specified as an optimal control problem with the daily intake as the control, which appears in a Michaelis-Menten relationship, for which there are well-developed procedures to follow. The best solution is determined by requiring minimum total intake under which the preassigned birth weight is reached. The algorithm has been generalized to the case where the fetal weight depends in a detailed way on the cumulative intake, suitably discounted according to the history. The optimality system is derived and then solved numerically using an iterative method for the specific values of parameter. The procedure is generic and can be adapted to any growth law and any parameterisation obtained by the detailed physiology.

The dynamics of tumor growth and cells pattern morphology.

The mathematical modeling of tumor growth is an approach to explain the complex nature of these systems. A model that describes tumor growth was obtained by using a mesoscopic formalism and fractal dimension. This model theoretically predicts the relation between the morphology of the cell pattern and the mitosis/apoptosis quotient that helps to predict tumor growth from tumoral cells fractal dimension. The relation between the tumor macroscopic morphology and the cell pattern morphology is also determined. This could explain why the interface fractal dimension decreases with the increase of the cell pattern fractal dimension and consequently with the increase of the mitosis/apoptosis relation. Indexes to characterize tumoral cell proliferation and invasion capacities are proposed and used to predict the growth of different types of tumors. These indexes also show that the proliferation capacity is directly proportional to the invasion capacity. The proposed model assumes: i) only interface cells proliferate and invade the host, and ii) the fractal dimension of tumoral cell patterns, can reproduce the Gompertzian growth law.