Exploring patterns of antibiotics during and after COVID-19 pandemic in wastewaters of northern Greece: Potential adverse effects on aquatic environment.

Antibiotics, recognized as Emerging Contaminants (ECs), have raised concerns due to their pervasive presence in wastewater treatment plants (WWTPs) and subsequent release into aquatic environments, posing potential ecological risks and contributing to the development of antibiotic-resistant genes. The COVID-19 pandemic prompted an unprecedented surge in antibiotic consumption, necessitating a comprehensive assessment of its impact on antibiotic levels in wastewater. In this light, a four-year monitoring study (2020-2023) was conducted in a WWTP located in the Northern Greece (Thessaloniki), employing High-Resolution Mass Spectrometry (HRMS) technology to monitor twenty antibiotics, during distinct phases pre-, during, and post-COVID-19. Our findings revealed that macrolides and fluoroquinolones were among the most often detected categories during the sampling period. Among the compounds detected, azithromycin and clarithromycin showed the most significant increases during the pandemic, doubling their average concentrations. This establishes a clear correlation between the rise in their concentrations and the incidence of COVID-19 cases. A general downward trend after 2021 was attributed to the new restrictions posed in Greece during this year, regarding the liberal prescription of antibiotics. Seasonal variation revealed a minute augmentation of antibiotics' use during the months that infections are increased. Additionally, the study highlights the ecological risks associated with elevated antibiotic presence and emphasizes the need for continued monitoring and regulatory measures to mitigate potential ecological repercussions. These findings contribute to our understanding of the complex interplay between antibiotic consumption, environmental presence, and the COVID-19 pandemic's impact on antibiotic pollution in WWTPs.

Does climbazole instigate a threat in the environment as persistent, mobile and toxic compound? Unveiling the occurrence and potential ecological risks of its phototransformation products in the water cycle.

Persistent, mobile, and toxic chemicals (PMT), such as the antimycotic climbazole-(CBZ), proliferate in water cycle and imperil drinking water quality, sparking off research about their environmental fate. Unlike the parent compound, its transformation products-(TPs) are scarcely investigated, much less as PMTs. To this end, phototransformation of CBZ was investigated. A novel suspect-screening workflow was developed and optimized by cross-comparing the results of the identified photo-TPs against literature data to create an enhanced HRMS-database for environmental investigations of CBZ/TPs in the water cycle. In total, 24 TPs were identified, 14 of which are reported for the first time. Isomerism, dechlorination, hydroxylation, and cleavage of the ether or C-N bond are suggested as the main transformation routes. A screening of CBZ/TPs was conducted in wastewater, leachates, surface, and groundwater, revealing a maximum concentration of 464.8 ng/L in groundwater. In silico and in vitro methods were used for toxicity assessment, indicating toxicity for CBZ and some TPs. Seemingly, CBZ is rightly considered as PMT, and a higher potential to occur in surface or groundwater than non-PM chemicals appears. Likewise, the occurrence of TPs due to PMT properties or emission patterns was evaluated.

Overarching issues on relevant pesticide transformation products in the aquatic environment: A review.

The intensification of agricultural production during the last decades has forced the rapid increase in the use of pesticides that finally end up in the aquatic environment. Albeit well-documented, pesticides continue to raise researchers' attention, because of their potential adverse impacts on the environment and, inevitably, humans. Once entering the aquatic bodies, pesticides undergo biotic and abiotic processes, resulting in transformation products (TPs) that sometimes are even more toxic than the parent compounds. A substantial shift of the scientific interest in the TPs of pesticides has been observed since their environmental fate, occurrence and toxicity is still in its formative stage. In an ongoing effort to expand the existing knowledge on the topic, several interesting works have been performed mostly in European countries, such as France, Germany, Italy, Switzerland, Greece, and Spain that counts the highest number of relevant publications. Pesticide TPs have been also studied to a lesser extent in Asia, North and South America. To this end, the main objective of this review is to delineate the global occurrence, fate, toxicity as well as the analytical challenges related to pesticide TPs in surface, ground, and wastewaters, with the view to contribute to a better understanding of the environmental problems related with TPs formation. The concentration levels of the TPs, ranging from the low ng/L to high µg/L scale and distributed worldwide. Ultimately, an attempt to predict the acute and chronic toxicity of TPs has been carried out with the aid of an in-silico approach based on ECOSAR, revealing increased chronic toxicity for the majority of the identified TPs, despite the change they underwent, while a small portion of them presented serious acute toxicity values.