Agency in urgency and uncertainty. Vaccines and vaccination in European media discourses.

Although Covid-19 was not the first pandemic, it was unique in the scale and intensity with which societies responded. Countries reacted differently to the threat posed by the new virus. The public health crisis affected European societies in many ways. It also influenced the way the media portrayed vaccines and discussed factors related to vaccine hesitancy. Europeans differed in their risk perceptions, attitudes towards vaccines and vaccine uptake. In European countries, Covid-19-related discourses were at the centre of media attention for many months. This paper reports on a media analysis which revealed significant differences as well as some similarities in the media debates in different countries. The study focused on seven European countries and considered two dimensions of comparison: between the pre-Covid period and the beginning of the Covid pandemic period, and between countries. The rich methodological approach, including linguistics, semantic field analysis and discourse analysis of mainstream news media, allowed the authors to explore the set of meanings related to vaccination that might influence actors' agency. This approach led the authors to redefine vaccine hesitancy in terms of characteristics of the "society in the situation" rather than the psychological profile of individuals. We argue that vaccine hesitancy can be understood in terms of agency and temporality. This dilemma of choice that transforms the present into an irreversible past and must be taken in relation to an uncertain future, is particularly acute under the pressure of urgency and when someone's health is at stake. As such, it is linked to how vaccine meaning is co-produced within public discourses.

Trust matters: The Addressing Vaccine Hesitancy in Europe Study.

This article presents the design of a seven-country study focusing on childhood vaccines, Addressing Vaccine Hesitancy in Europe (VAX-TRUST), developed during the COVID-19 pandemic. The study consists of (a) situation analysis of vaccine hesitancy (examination of individual, socio-demographic and macro-level factors of vaccine hesitancy and analysis of media coverage on vaccines and vaccination and (b) participant observation and in-depth interviews of healthcare professionals and vaccine-hesitant parents. These analyses were used to design interventions aimed at increasing awareness on the complexity of vaccine hesitancy among healthcare professionals involved in discussing childhood vaccines with parents. We present the selection of countries and regions, the conceptual basis of the study, details of the data collection and the process of designing and evaluating the interventions, as well as the potential impact of the study. Laying out our research design serves as an example of how to translate complex public health issues into social scientific study and methods.

Researching childhood vaccine hesitancy in the wake of COVID-19.

The COVID-19 pandemic has had a seismic effect on public healthcare, vaccine production, and on society. However, the pandemic has also had a methodological impact on social researchers, including those seeking to better understand vaccine hesitancy in relation to childhood vaccines. In this short communication, we describe the challenging experience of recruiting and conducting qualitative interviews with UK healthcare professionals and vaccine hesitant parents in early 2022. We also explore the way in which the context of COVID influenced our data analysis. Finally, we make recommendations for how researchers, including those using qualitative or quantitative methods, might learn from our experiences, as the complex and delicate relationship between society and vaccines continues to evolve in the wake of the pandemic.

Recruiting a Hard-to-Reach, Hidden and Vulnerable Population: The Methodological and Practical Pitfalls of Researching Vaccine-Hesitant Parents.

While recruitment is an essential aspect of any research project, its challenges are rarely acknowledged. We intend to address this gap by discussing the challenges to the participation of vaccine-hesitant parents defined here as a hard-to-reach, hidden and vulnerable population drawing on extensive empirical qualitative evidence from seven European countries. The difficulties in reaching vaccine-hesitant parents were very much related to issues concerning trust, as there appears to be a growing distrust in experts, which is extended to the work developed by researchers and their funding bodies. These difficulties have been accentuated by the public debate around COVID-19 vaccination, as it seems to have increased parents' hesitancy to participate. Findings from recruiting 167 vaccine-hesitant parents in seven European countries suggest that reflexive and sensible recruitment approaches should be developed.

Regional and neighbourhood-based variation in three types of vaccine attitude in Britain.

This study investigates the geographic patterning of attitudes towards vaccination in Britain (England, Wales, and Scotland). Using survey data from the 2018 Wellcome Monitor linked to lower super output areas and NUTS2 regions, this study provides evidence that negative attitudes regarding the risk of side-effects from vaccination and regarding the efficacy of vaccination are correlated with higher levels of local deprivation. While previous research has highlighted associations between vaccine uptake and deprivation for a variety of reasons, this study specifically contends that particular individualised types of caution about vaccines also tend to cluster in deprived areas.

Two Worlds in One: What 'Counts' as Animal Advocacy for Veterinarians Working in UK Animal Research?

The concept of advocacy is of increasing importance to the veterinary profession internationally. However, there are concerns around the ambiguity and complexity of acting as an advocate in practice. This paper explores what 'animal advocacy' involves for veterinarians working in the domain of animal research, where they are responsible for advising on health and welfare. In focusing on the identity of veterinarians working in an arena of particular contestation, this paper provides empirical insights into how veterinarians themselves perform their role as an 'animal advocate'. Analysing interview data with 33 UK 'Named Veterinary Surgeons', this paper therefore examines what 'counts' as animal advocacy for veterinarians, considering the way their role as animal advocate is performed. Focusing on the themes of 'mitigating suffering', 'speaking for', and 'driving change' as three central ways in which veterinarians working in animal research facilities act as animal advocates, we draw out some of the complexities for veterinarians working in areas where animal care and harm coexist. Finally, we conclude by calling for further empirical exploration of animal advocacy in other veterinary domains and for more critical attention to the wider social systems which produce the need for such advocacy.

Animal research, ethical boundary-work, and the geographies of veterinary expertise.

The veterinary profession has been relatively understudied in social science, though recent work has highlighted the geographic dimensions of veterinary expertise. This paper draws on in-depth qualitative interviews with Named Veterinary Surgeons (NVSs) working in UK animal research to demonstrate how and why they distinguish between ethical aspects of veterinary work in the spaces of the laboratory and general clinical practice. The paper mobilises the sociological concept of ethical boundary-work to help understand how animal research - often assumed to represent a contentious ethical space - is constructed positively as a space for veterinary work. Findings suggest first, that NVSs differentiate between laboratory veterinary-work and clinical work based on the scale at which veterinary expertise functions in the provision of healthcare to animals. Second, NVSs highlight a geography of veterinary authority in which veterinary expertise is felt to be more successfully applied in the laboratory compared with the clinic, where professional expertise competes with other sources of information and clients' finances and behaviours. Third, NVSs articulate a geography of consistency in which veterinary care in the laboratory is claimed to be more consistent between animals, as opposed to in the clinic, where animal experience may be influenced by individual owner characteristics. Overall, we show how through engaging in this kind of ethical boundary-work NVSs are not only presenting a form of scientific practice as 'ethical', they are also constructing a professional topology of veterinary practice and expertise. Finally, the paper argues for greater attentiveness to veterinary geographies beyond the more routine spaces of veterinary practice.

Attitudes towards vaccination and knowledge about antibiotics: Analysis of Wellcome Monitor survey data.

Vaccine hesitancy and antimicrobial resistance are biomedically connected public health challenges, but to date minimal research has examined social connections between the UK public's attitudes towards vaccination and attitudes towards antibiotic use. Understanding the extent to which these issues are attitudinally parallel would be valuable for implementing and evaluating public health interventions. Using data from the Wellcome Trust Monitor Wave 4 this study examined social associations between these two areas. An ordinal logistic regression model predicting knowledge level about antibiotics was fitted using 2,654 observations, controlling for known outcome covariates, with perceptions of the risk of side-effects from vaccination and of the efficacy of vaccination as a preventative intervention as independent variables. Compared to the modal response category of 'Fairly low', respondents who rated the risk of serious side-effects from vaccination as 'Very high' (OR = 2.87, 95% CI = 1.71-4.89) or 'Fairly high' (OR = 1.51, 95% CI = 1.21-1.88) were more likely to have provided incorrect responses to questions about the utility of antibiotics for treating different types of infection. Conversely, respondents who felt there was 'No risk at all' (OR = 0.69, 95% CI = 0.50-0.95) were less likely to have provided incorrect responses about the utility of antibiotics. Compared to the modal category of 'Almost always effective', only respondents who felt that vaccines were 'Sometimes effective' (OR = 1.26, 95% CI = 1.05-1.51) or 'Almost never or never effective' (OR = 2.32, CI = 1.32-4.19) were more likely provide incorrect responses regarding antibiotics' utility. Negative perceptions of vaccination and misperceptions about the role of antibiotics for treating infections are associated with one other within the general UK public. Qualitative research is needed to understand the nature of this association and identify areas of public understanding that are not exclusive to specific health interventions but that may be targeted to improve responsiveness to vaccine- and antibiotic-related public health interventions.

"Refugees from practice"? Exploring why some vets move from the clinic to the laboratory.

BACKGROUND: Named veterinary surgeons (NVSs) are a mandated presence in licensed animal research establishments in the UK. Some NVSs come into their laboratory roles having left general veterinary practice, which is currently facing significant recruitment and retention challenges. Understanding the factors that motivate veterinary professionals to move from practice to laboratory roles provides insight into the issues underlying recruitment and retention challenges in veterinary practice. METHODS: Qualitative semi-structured interviews with 33 NVSs were conducted in-person or over the telephone. The interviews were transcribed, anonymised and analysed using an inductive approach. RESULTS: Participants' accounts of their career trajectories generally emphasised push factors motivating them to leave practice, rather than pull factors to move into a laboratory role: Indeed, many participants recalled originally having little knowledge of the NVS role upon discovering it. The push factors recounted by interviewees strongly reflect the factors identified in recent research into recruitment and retention in the veterinary profession, such as business concerns and poor work-life balance. CONCLUSION: This study shows that laboratory animal work is often considered by NVSs as more manageable or fulfilling than practice work. To improve retention, the push factors identified by NVSs should be addressed in practice management and veterinary pedagogy.

When research animals become pets and pets become research animals: care, death, and animal classification.

This paper explores what happens to care, and decisions about ending and extending life, when research animals become pets and pets become research animals. To do this, we draw on in-depth qualitative research on (i) rehoming of laboratory animals, (ii) veterinary clinical research, and (iii) the role of the Named Veterinary Surgeon (NVS) in UK animal research. We begin by exploring how (in theory and practice) the ethical, affective, and practical elements of care are split in the research laboratory. We then investigate arguments for and against ending and extending animal life via clinical research and rehoming, highlighting how these activities bring norms and dilemmas around animal death in the laboratory and veterinary clinic to the fore. We conclude by demonstrating the value of investigating borders between animal categories for understanding dilemmas around care and death, and for contributing to emerging literatures within geography around animal care, death, and categorisation. Key contributions of our work include highlighting: how care roles can be split; the importance of considering speculative and in-practice elements of care; the context-dependency and multiplicity of practices of killing in the veterinary clinic and laboratory; and the flexibility and changing nature of animal categories.

Este artículo explora lo que sucede con el cuidado y las decisiones sobre el final y la extensión de la vida cuando los animales de investigación se convierten en mascotas y las mascotas se convierten en animales de investigación. Para hacer esto, nos basamos en una investigación cualitativa a profundidad sobre (i) el realojamiento de animales de laboratorio, (ii) la investigación clínica veterinaria y (iii) el papel del Cirujano Veterinario Designado (CVD o NVS por sus siglas en inglés) en la investigación con animales en el Reino Unido. Comenzamos explorando cómo (en la teoría y la práctica) los elementos éticos, afectivos y prácticos del cuidado se dividen en el laboratorio de investigación. Luego investigamos los argumentos a favor y en contra de terminar y extender la vida animal a través de la investigación clínica y el realojamiento, destacando cómo estas actividades ponen de manifiesto las normas y los dilemas en torno a la muerte animal en el laboratorio y la clínica veterinaria. Concluimos demostrando el valor de investigar las fronteras entre las categorías de animales para comprender los dilemas sobre el cuidado y la muerte, y para contribuir a las literaturas emergentes dentro de la geografía sobre el cuidado, la muerte y la categorización de los animales. Las contribuciones clave de nuestro trabajo incluyen destacar: cómo se pueden dividir los roles de cuidado; la importancia de considerar elementos especulativos y de práctica de la atención; la dependencia del contexto y la multiplicidad de prácticas de matanza en la clínica veterinaria y el laboratorio; y la flexibilidad y naturaleza cambiante de las categorías de animales.

Cet article étudie ce qu'il advient du care et des décisions concernant l'arrêt ou l'extension de la vie, quand les animaux de recherche deviennent animaux de compagnie et que les animaux de compagnie deviennent animaux de recherche. Pour ce faire, nous nous appuyons sur une recherche qualitative approfondie sur (i) l'adoption d'animaux de laboratoire (ii) la recherche clinique vétérinaire, et (iii) le rôle du chirurgien vétérinaire nommé (NVS - Named Veterinary Surgeon) dans la recherche animale au Royaume-Uni. Nous commençons en explorant la manière dont, en théorie et en pratique, les éléments éthiques, affectifs et pratiques du care sont divisés dans le laboratoire de recherche. Nous passons ensuite en revue les arguments pour ou contre l'arrêt ou l'extension de la vie animale par le biais de la recherche clinique et l'adoption, en soulignant la façon dont ces activités amènent au premier plan les normes et les dilemmes autour de la mort animal en laboratoire et dans les cliniques vétérinaires. Nous concluons en démontrant la valeur de l'étude des frontières entre les catégories d'animaux pour comprendre les problèmes entourant le care et la mort, et pour contribuer aux recherches naissantes dans la géographie concernant le care, la mort et la catégorisation des animaux. Les contributions majeures de nos travaux comprennent la mise en évidence de: la manière dont les rôles de care peuvent être divisés; l'importance de la prise en compte d'éléments de care spéculatifs et dans la pratique ; le rapport au contexte et la multiplicité des pratiques de mise à mort dans les cliniques et les laboratoires vétérinaires ; et la flexibilité et la nature changeante des catégories d'animaux.

Analysing incompliant attitudes towards antibiotic prescription completion in the UK.

OBJECTIVES: To analyse demographic, social and geographic predictors of incompliant attitudes towards prescription completion in the UK. METHODS: Two waves of the Eurobarometer survey (85.1 and 90.1) were analysed, with a final sample size of 2016. Using logistic regression, the best-fitting combination of a set of identified variables was specified. The regression output and the model-averaged importance of each variable were analysed. RESULTS: Compared with a median prevalence region, respondents in the Nomenclature of Territorial Units for Statistics (NUTS) 1 London (OR = 2.358, 95% CI = 1.100-5.398) and Scotland (OR = 2.418, 95% CI = 1.083-5.693) regions were most likely to report an incompliant attitude. Respondents who correctly answered questions about whether unnecessary use of antibiotics could make them ineffective in future (OR = 0.353, 95% CI = 0.230-0.544), whether antibiotics kill viruses (OR = 0.644, 95% CI = 0.450-0.919) and whether antibiotics treat colds (OR = 0.412, 95% CI = 0.287-0.591) were less likely to report incompliant attitudes. Conversely, respondents who correctly responded that antibiotics can cause side effects (OR = 1.419, 95% CI = 1.014-1.999) were more likely to report incompliant attitudes. There was some evidence of associations between political orientation and level of compliance. Uncooperative survey respondents (OR = 2.001, 95% CI = 1.108-3.526) were more likely to report incompliant attitudes. CONCLUSIONS: Incompliant attitudes towards antibiotic prescription compliance in the UK are associated with a variety of factors, including regional geographic variation in attitudes. Knowledge about antibiotics can relate to good stewardship attitudes, but concerns over side effects are associated with poor attitudes. Further research should examine the underlying attitudes and beliefs that political orientation may be a marker for in the context of antibiotic stewardship. Survey samples reliant on self-selection are likely to be biased towards good stewardship.

Online health information and public knowledge, attitudes, and behaviours regarding antibiotics in the UK: Multiple regression analysis of Wellcome Monitor and Eurobarometer Data.

BACKGROUND: Antimicrobial resistance is a global public health problem with some socially patterned drivers. The objective of the study was to investigate associations between use of and trust in the Internet as a source of health-related information and the public's knowledge, attitudes, and behaviours regarding antibiotics. METHODS: Two representative cross-sectional surveys (the 2015 Wellcome Monitor (n = 1524) and UK segment (n = 1330) of the 2016 Eurobarometer 85.1) covering knowledge about antibiotics and antibiotic consumption were analysed. Knowledge, attitude, and behaviour variables were analysed using regression in relation to demographic characteristics and use and trust in the Internet as a source of information. RESULTS: The key findings of the analysis are that both use of the Internet as a source of medical research information (variable from the Wellcome Monitor) and trust in the Internet as a source of information about antibiotics (variable from the Eurobarometer) were independently and positively associated with knowledge, attitude, and behaviour regarding antibiotics. Additionally, knowledge about antibiotics was positively associated with behaviour with antibiotics (Wellcome Monitor) and attitude towards finishing antibiotic prescriptions (Eurobarometer). Higher levels of education were associated with better knowledge about antibiotics in both datasets. Older age was positively associated with behaviour and attitude regarding antibiotic consumption. CONCLUSIONS: The Internet is a resource for disseminating quality health information that has the potential to improve stewardship of antibiotics in the community. This study suggests that members of the UK public that use the Internet as a source of health-related information are more likely to be better informed about, and be more responsible with, antibiotics. This mode of information dissemination should be capitalised on to improve antimicrobial stewardship, and further research should examine what the most effective online information sources are in the UK and to what extent their association with behaviour is causal.

Phenotypic variation of lipid composition in Burkholderia cepacia: a response to increased growth temperature is a greater content of 2-hydroxy acids in phosphatidylethanolamine and ornithine amide lipid.

Burkholderia cepacia produces an unusual range of polar lipids, which includes two forms each of phosphatidylethanolamine (PE) and ornithine amide lipid (OL), differing in the presence or absence of 2-hydroxy fatty acids. By using chemostat cultures in chemically defined media, variations in the lipid content and the proportions of individual lipids have been studied as a function of (a) growth temperature, (b) growth rate and (c) growth-limiting nutrient (carbon, magnesium, phosphorus or oxygen). Total cellular lipid in carbon-limited cultures was lowest at high growth temperatures and low growth rates. Increases in growth temperature over the range 25-40 degrees C led to increases in the proportions of molecular species of PE and OL containing 2-hydroxy acids, without changing the PE:OL ratio. Growth temperature did not alter the balance between neutral and acidic lipids, but the contribution of phosphatidylglycerol to the latter increased with rising growth temperature and growth rate. Pigmentation of cells and the presence of flagella were also temperature-dependent. Change in growth rate also affected the PE:OL ratio and the extent to which monoenoic acids were replaced by their cyclopropane derivatives. Whereas similar lipid profiles were found for carbon-, magnesium- and oxygen-limited cultures, ornithine amides were the only polar lipids detected in phosphorus-limited cells.