ABSTRACT
The experiments on mice showed that subchronic food restriction to 40 and 8% of unrestricted ration is a strong stressor inducing devastation of lymphoid organs, primarily the thymus and spleen. The mice in the group with severe food restriction (8% of normal ration) demonstrated increased front paw grip force. We also observed an increase in spontaneous motor activity in these animals correlated with food restriction. Food deprivation led to inhibition of proliferative activity of the bone marrow cells and suppression of erythropoiesis. Moreover, severe food restriction was accompanied by a decrease in the number of double-strand DNA breaks evaluated by the release of γH2AX+-cells and the ratio of polychromatophilic erythrocytes.
Subject(s)
Food Deprivation/physiology , Thymus Gland/metabolism , Animals , Body Weight/physiology , Bone Marrow Cells/metabolism , Chromosomes/metabolism , Erythrocytes/metabolism , Erythropoiesis/physiology , Female , MiceABSTRACT
Experiments showed that drinking water supplemented with a complex of 12 microelements and organic acids stimulates body mass gain and strengthening of radioresistance in mice. Obviously, availability of water with the mineral and organic complex before and after irradiation was the factor in survival of a part of mice exposed to the absolutely lethal dose (7.6 Gy from gamma-quanta 60Co) and reduction of chromosomal aberrations rate in bone marrow cells in consequence of nonlethal (1 Gy) irradiation.
Subject(s)
Bone Marrow Cells/pathology , Chromosome Aberrations/radiation effects , Gamma Rays/adverse effects , Minerals/pharmacology , Radiation Injuries, Experimental/prevention & control , Radiation-Protective Agents/pharmacology , Animals , Bone Marrow Cells/radiation effects , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Radiation Injuries, Experimental/metabolism , Radiation Injuries, Experimental/pathologyABSTRACT
Effect of potable water with low content of heavy stable isotopes of oxygen and hydrogen on body mass and hematopoiesis was studied in intact laboratory animals. Outbred CD-I and first generation hybrid (CBA*C57B)F1 mice exhibited a statistical acceleration of body mass gain as a result of drinking rectified light-isotopic water with ppm 35; stimulating effect was noticed with respect to peripheral blood parameters and condition of the hematopoietic organs. The parameters under study did not go beyond boundaries of the physiological norm.
Subject(s)
Drinking Water/administration & dosage , Animals , Blood Platelets/drug effects , Body Mass Index , Bone Marrow Cells/drug effects , Cell Count , Crosses, Genetic , Erythrocytes/drug effects , Hematocrit , Hemoglobins/analysis , Hydrogen/metabolism , Isotopes/metabolism , Leukocytes/drug effects , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Oxygen Isotopes/metabolism , Weight Gain/drug effectsABSTRACT
The propose of these examination was the study of antiradiation effects of vaccine Grippol (VG). It was determined, that subcutaneous injection VG mice (0.2 ml), hamsters (0.2 ml), dogs (0.5 ml) before 1-14 days of total gamma-irradiation (2.5-8.5 Gy) had positive effect of acute radiation disease. This effect was expressed in increase of survival the mice and hamsters on 30-60%, increase number cells in bone marrow and neutrophiles, erythrocytes, lymphocytes and hemoglobin in blood by comparison with control mice. VG stimulated increase of contents of leucocytes in blood of irradiated (2.5 Gy) dogs.
Subject(s)
Gamma Rays , Influenza Vaccines/administration & dosage , Radiation Injuries, Experimental/prevention & control , Radiation-Protective Agents/administration & dosage , Animals , Bone Marrow/immunology , Cricetinae , Dogs , Injections, Subcutaneous , Leukocyte Count , Lymphocytes/immunology , Mice , Neutrophils/immunology , Radiation Injuries, Experimental/blood , Radiation Injuries, Experimental/immunologyABSTRACT
The possibility of the successful modification of radiation injury by chitosan with low molecular weight (10 kDa) has been established under experimental conditions. The survival of mice increased up to 72.7 and 44.7% respectively at intravenous and intramuscular injection 30 min before gamma-irradiation with a dose 8 Gy (LD97). In guinea pigs the effect was 50-52.6% at intravenous and 40% at intramuscular administration 1-3 h after irradiation with a dose 5 Gy (LD90). Radioprotective efficiency of 10 kDa chitosan is close to that of high-molecular-weight (65-70 kDa) preceding (medicine RS-10 and RS-11).
Subject(s)
Chitosan/therapeutic use , Radiation Injuries, Experimental/drug therapy , Radiation-Protective Agents/therapeutic use , Animals , Chitosan/administration & dosage , Chitosan/chemistry , Guinea Pigs , Male , Mice , Molecular Weight , Radiation Injuries, Experimental/prevention & control , Radiation-Protective Agents/administration & dosage , Radiation-Protective Agents/chemistryABSTRACT
In experimental conditions the radioprotective properties of the placental complex Luplatex created in Scientific production complex "Biotechindustry" was studied. In experiments on mice F1(CBA x C57Bl) it was shown that Luplatex injected intraperitoneally in dose 0.5 ml 5-10 min before or after whole body gamma-irradiation with 8 Gy (LD80/30) increased the survival up to 40% as compared to the control group. In white mice protected by oral administration of Luplatex 30 min before exposure to 7.5 Gy, which is absolute minimal lethal dose for this type of mice (LD100/30), the effect was 48.3%.
Subject(s)
Radiation-Protective Agents/pharmacology , Administration, Oral , Animals , Dose-Response Relationship, Radiation , Male , Mice , Radiation Injuries, Experimental/prevention & control , Radiation-Protective Agents/administration & dosageABSTRACT
The influence of chitosan molecular mass (70, 10 and 5 kDa) on its radioprotective efficiency in mice experiments was studied. It was shown that chitosans with molecular masses of 70 and 10 kDa had similar radioprotective properties. The survival of mice increased up to 73% and 87% respectively at intravenous injection 15-30 min before a whole-body exposure to 137Cs gamma-radiation at a dose of 8 Gy (Cd97/30). Practically absolute loss of radioprotective activity occurred below a threshold of about 10 kDa. The results showed a high chitosan radioprotective activity in a wider range of molecular masses than it was supposed earlier.
Subject(s)
Chitin/analogs & derivatives , Chitin/administration & dosage , Chitin/chemistry , Radiation-Protective Agents/administration & dosage , Whole-Body Irradiation , Animals , Chitosan , Injections, Intravenous , Male , Mice , Molecular Weight , Radiation-Protective Agents/chemistry , Survival AnalysisABSTRACT
The experiments with M. fasciculata monkeys exposed to 137Cs gamma-radiation with a dose of 6.9 Gy showed that Latranum, a blocker of serotonin 5-HT3 receptors, is a more efficient antiemetic than Dimetphramidum, a D2 dophamin lytic. This is suggested by fewer animals with emetic reaction of by less severe vomiting in case they have any. The results agree well with a hypothesis that serotonin receptors are dominant in the chemoreceptor trigger zone of monkeys.
Subject(s)
Antiemetics/pharmacology , Benzamides/pharmacology , Dopamine Antagonists/pharmacology , Dopamine D2 Receptor Antagonists , Radiation Injuries, Experimental/complications , Receptors, Serotonin/drug effects , Serotonin Antagonists/pharmacology , Vomiting/etiology , Animals , Antiemetics/therapeutic use , Benzamides/therapeutic use , Disease Models, Animal , Dopamine Antagonists/therapeutic use , Drug Evaluation, Preclinical , Female , Gamma Rays , Macaca fascicularis , Male , Radiation Injuries, Experimental/drug therapy , Receptors, Dopamine D2/drug effects , Receptors, Dopamine D2/radiation effects , Receptors, Serotonin/radiation effects , Receptors, Serotonin, 5-HT3 , Serotonin Antagonists/therapeutic use , Time Factors , Vomiting/drug therapy , Whole-Body IrradiationABSTRACT
We present the results of a long-standing experimental development of ways and means for acute radiation sickness treatment that have been authorized for application in medicine and are mostly aimed at large-scale accidental injuries. The paper describes means for early treatment (prodigiosan, desoxynate, typhoid vaccine, proteus vaccine), a myeolopoiesis stimulant (estradiol dipropionate), a detoxication procedure (hemosorption), substitution therapy with bone marrow cells and peripheral blood mononuclears, anti-infectious schemes comprising antibiotics and polyvitamins.
Subject(s)
Radiation Injuries, Experimental/therapy , Acute Disease , Animals , Combined Modality Therapy/trends , Dogs , HumansABSTRACT
Following radiation damage from LD50-LD97, changes in blood, immune and endocrine parameters were revealed and followed up in dogs at the time of late effects development, 3-18 months after exposure. The changes result from post-radiation immunodeficiency and resemble those observed in residents of radioactive contaminated areas or in men participated in Chernobyl accident amelioration.
Subject(s)
Homeostasis/radiation effects , Radiation Injuries, Experimental/physiopathology , Animals , Dogs , Endocrine Glands/radiation effects , Female , Hematopoiesis/radiation effects , Humans , Immune System/radiation effects , Male , Power Plants , Radiation Injuries, Experimental/blood , Radiation Injuries, Experimental/immunology , Radioactive Hazard Release , Time Factors , UkraineABSTRACT
Simulation of cytopenia by injection of cyclophosphamide (100 mg/kg) or by exposure to ionizing radiation (4 Gy) was shown to cause in mice similar, by the severity and rate of development, transient inhibition of haemopoiesis which was somewhat more persistent after irradiation. Glucosaminylmuramyl dipeptide applied after the above effects promoted the haemopoiesis recovery.
Subject(s)
Acetylmuramyl-Alanyl-Isoglutamine/analogs & derivatives , Adjuvants, Immunologic/therapeutic use , Hematopoiesis/drug effects , Pancytopenia/drug therapy , Acetylmuramyl-Alanyl-Isoglutamine/therapeutic use , Animals , Cyclophosphamide , Drug Evaluation, Preclinical , Gamma Rays , Hematopoiesis/radiation effects , Male , Mice , Mice, Inbred C57BL , Mice, Inbred CBA , Pancytopenia/blood , Pancytopenia/etiology , Time FactorsABSTRACT
A typhoid vaccine with sexta-anatoxin delivered to mice 4.5-5 h after gamma-irradiation has a pronounced therapeutic effect: survival rate is 42% with radiation dose of 8.2 Gy (LD85/30) and 19% with radiation dose of 8.7 Gy (LD95/30). The vaccine of 2.5 and 5 mg/kg combined with methotrexate has a more pronounced therapeutic effect increasing the survival rate up to 65% (LD85/30) and up to 35-40% (LD95/30).