Effect of semen processing methods on lumpy skin disease virus status in cryopreserved bull semen.

Lumpy skin disease is an economically important disease of cattle, caused by the lumpy skin disease virus (LSDV; Capripoxvirus). It has a variable clinical appearance but, in severely affected animals, is associated with extensive skin damage, pneumonia and death. The LSDV can be found in the semen of infected bulls for prolonged periods of time, from where it can be transmitted by mating or artificial insemination and cause clinical disease in heifers and cows. In this study, an ejaculate was collected from a LSDV seronegative bull and confirmed free from LSDV DNA by PCR. The ejaculate was split into a control sample (C), a sample spiked with a 4 log TCID50 dose of an LSDV isolate (HD) and a 103 dilution of the virus suspension (ND) and frozen routinely. Two straws from each of the different semen treatment groups (HD, ND and C) were subsequently thawed and subjected to swim-up, single layer centrifugation, Percoll® density gradient and a Percoll® density gradient with added trypsin. For one set of straws, semen quality variables were recorded, and viral DNA status determined using PCR; the other set was used for positive staining electron microscopy. Samples determined to be positive for LSDV DNA by PCR were then subjected to virus isolation (VI). Complete elimination of LSDV from semen did not occur with use of any of the processing methods. Trypsin did reduce the viral load, and eliminated LSDV from the ND sample, but severely negatively influenced semen quality. The LSDV virions, as assessed by electron microscopy, were associated with the sperm plasma membrane. Further investigation is needed to establish the efficacy of immuno-extenders for rendering semen free from LSDV.

Training Method and Other Factors Affecting Student Accuracy in Bovine Pregnancy Diagnosis.

To optimize bovine pregnancy diagnosis (PD) training, factors influencing student performance were investigated. The objective was to determine whether training method, gender, background (farm, urban, or mixed), previous experience in bovine PD, and current career interest influenced the accuracy of bovine PD by trans-rectal palpation (TRP). Fourth-year (of a 6-year program) veterinary students (n=138) received one PD training session in groups using either simulator training on Breed'n Betsy (BB) or training on live cows (C). Students completed a questionnaire on gender, background, and career interest. Students' PD accuracy (pregnancy status and stage) was determined after training when each student palpated six cows with known pregnancy status. Students' accuracy in determining pregnancy status was measured as sensitivity and specificity (the ability to correctly identify the presence and absence of pregnancy respectively). Factors that influenced overall accuracy with a higher student sensitivity of bovine PD by TRP were training method, farming background, an interest in a mixed animal career, and stage of gestation. Gender of students and previous experience in bovine PD did not have an influence. Training on BB simulators was associated with lower student sensitivity for pregnancy detection in cows <6 months pregnant. Student sensitivity for pregnancy detection in cows >6 months pregnant was similar for training on BB simulators and live cows. No evaluated factors were significantly associated with specificity of PD. Teaching efforts focusing on specificity of PD and repeated simulator-based training in conjunction with live cow exposure are recommended.

Curricular Renewal at the Southern Tip of Africa: The 2016 Veterinary Curriculum at the University of Pretoria.

Four years after the institution of a new curriculum at the University of Pretoria's Faculty of Veterinary Science, a second major curricular renewal was initiated as a result of several pressing drivers, many of which were unique to the national and institutional context. During the project, South African higher education was disrupted by student protests prompted by financial pressures on students, the overt colonial structure of higher education, and the need to accelerate transformation of the sector. A structured curricular renewal process was followed, including the laying down of the principles, the structuring the macro-curriculum as a story, the design of a meso- and micro-curriculum, and the mapping of the curriculum to Day One outcomes. The resulting program is a 6-year bachelor's degree with a blend of discipline and species modules, with the first cohort graduating in 2016. There is a strong focus on skills embodied in a 62-week experiential component, managed using a unique custom-designed online platform for booking placements, documenting exposure, assessing competency, and providing feedback to students. The experiential training includes a large elective component. Several causes of loss of impetus during the process are discussed and proposals are made for avoiding these. The value of accreditation as a driver and a source of inputs is evident. The process has succeeded in producing a significantly reshaped curriculum that has been well received by external stakeholders.