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1.
Anal Biochem ; 583: 113360, 2019 10 15.
Article in English | MEDLINE | ID: mdl-31288000

ABSTRACT

The performance indicator called limit of detection for microarray platform (LODP) was defined in ISO 16578:2013. The methods to determine practical LODP were explored. In general, + 3 SD of the background is used as the signal strength of limit of detection and criteria for dividing positive and negative results. Since the negative signal had been defined differently for each microarray platform, signals obtained from Non-Probe Spots (NPS) installed on the microarrays were defined as the "background" of microarrays. LODP was determined as the lowest concentration of which the average signal exceeded Avg. + 3 SD of the background (NPS) and the signal was significantly different from those of the lower and higher adjacent concentration points measured with a diluted series of reference materials. For reliable qualitative analysis, the positive results can be defined as signals higher than those corresponding to LODP and negative results as lower signals, without determining limit of detection for all target probes. The use of LODP also enables comparisons of platform performances without checking sequence dependencies, and assists to select reliable and fitting platforms for experimental purposes.


Subject(s)
Gene Expression Profiling/methods , Limit of Detection , Oligonucleotide Array Sequence Analysis/methods , RNA/analysis , Reproducibility of Results
2.
Kyobu Geka ; 67(2): 121-4, 2014 Feb.
Article in Japanese | MEDLINE | ID: mdl-24743481

ABSTRACT

Endoscopic vein harvesting (EVH) for coronary artery bypass grafting has been proved to be effective in reduced wound complications, without compromising long-term mortality or composite of death, myocardial infarction and repeat revascularization. However, only disposable devices that are costly and raise environmental problems, have been obtained on the market in Japan. Now, a non-disposable device for EVH is available. With this equipment, a 50 cm-long great saphenous vein can be obtained with a single 3 cm-long incision. We demonstrate how to use this device and show its tips and pitfall.


Subject(s)
Endoscopy , Saphenous Vein/surgery , Tissue and Organ Harvesting/instrumentation , Coronary Artery Bypass , Equipment Reuse , Humans
3.
Nihon Shokakibyo Gakkai Zasshi ; 110(12): 2089-99, 2013 Dec.
Article in Japanese | MEDLINE | ID: mdl-24305097

ABSTRACT

We report a rare case of duplication of the transverse colon in an adult. A 26-year-old male presented to our hospital with upper left abdominal pain and fever. An abdominal computed tomography scan revealed an inflammatory mass in the gastrocolic ligament. Although conservative management with fasting and antibiotics ameliorated the abdominal pain, the mass remained approximately 5cm at its largest diameter. Further investigation with a Gastrografin(®) enema revealed a fistula in the central part of the transverse colon. A perforation of the transverse colon was intraoperatively detected; therefore, both the intra-abdominal abscess and part of the transverse colon were excised. Pathological examination revealed duplication of the transverse colon that was lined with ciliated columnar epithelium, squamous epithelium, and heterotopic glands.


Subject(s)
Colon, Transverse/abnormalities , Colon, Transverse/pathology , Intestinal Mucosa/pathology , Adult , Humans , Male
4.
Phys Rev Lett ; 111(14): 144102, 2013 Oct 04.
Article in English | MEDLINE | ID: mdl-24138241

ABSTRACT

Coupled dynamical systems with one slow element and many fast elements are analyzed. By averaging over the dynamics of the fast variables, the adiabatic kinetic branch is introduced for the dynamics of the slow variable in the adiabatic limit. The dynamics without the limit are found to be represented by stochastic switching over these branches mediated by the collective chaos of the fast elements, while the switching frequency shows a complicated dependence on the ratio of the two time scales with some resonance structure. The ubiquity of the phenomena in the slow-fast dynamics is also discussed.


Subject(s)
Nonlinear Dynamics , Stochastic Processes
5.
Nihon Shokakibyo Gakkai Zasshi ; 105(9): 1353-61, 2008 Sep.
Article in Japanese | MEDLINE | ID: mdl-18772576

ABSTRACT

TEN is a severe dermatological disorder characterized by extended epidermal necrosis. Disseminated mucosal erosions have been occasionally reported to occur in the gastrointestinal tract. We report a case of toxic epidermal necrolysis (TEN) with severe intestinal manifestation. A 52-year-old woman was admitted with high fever, skin eruption and severe diarrhea. She was diagnosed as toxic epidermal necrolysis (TEN), which was most likely due to nonsteroidal anti-inflammatory drugs (NSAIDs). After skin lesion recovered, fever and bloody diarrhea went on. Colonoscopy and X-ray revealed lead-pipe like stenotic long loops which was caused by erosion and sublation of whole mucosa of large intestinum, and severe stenosis of ileum end. The ileo-cecal region was resected on the 216(th) hospital day. Pathological examination showed sublation of mucoepithelium and inflammatory change in the muco-submucosal layer, but muscular layers of mucosa remained intact. Pathologically, the intestinal lesion resemble the changes in the early skin lesions and seems to be part of the systemic lesion of TEN.


Subject(s)
Intestines/pathology , Stevens-Johnson Syndrome/pathology , Anti-Inflammatory Agents, Non-Steroidal/adverse effects , Stevens-Johnson Syndrome/etiology
6.
Ann Thorac Cardiovasc Surg ; 11(5): 343-5, 2005 Oct.
Article in English | MEDLINE | ID: mdl-16299465

ABSTRACT

An 87-year-old man underwent aorto-bifemoral bypass using a bifurcated Bionit II knitted Dacron graft for high aortic occlusion in 1987 at another hospital. In November, 2004, he was admitted to our institution because of difficulty in walking due to swelling and tenderness in the right groin. Computed tomography (CT) scan indicated bilateral aneurysms of the grafts in the groins. The size of the right and left aneurysms were 73 mm and 52 mm, respectively. Angiography showed some extravasation in the right thigh. We performed surgical replacement of all the dilated parts with new ringed-Dacron grafts. We report a rare case of late rupture of bifurcated Sauvage Bionit II Dacron graft.


Subject(s)
Blood Vessel Prosthesis , Femoral Artery/surgery , Polyethylene Terephthalates , Prosthesis Failure , Aged, 80 and over , Groin , Humans , Male , Rupture
7.
Ann Thorac Cardiovasc Surg ; 11(3): 198-200, 2005 Jun.
Article in English | MEDLINE | ID: mdl-16030481

ABSTRACT

We report a rare case of severe hemolytic anemia accompanied by moderate renal insufficiency after mitral valve repair. Although the degree of the residual mitral regurgitation was less than 1+ during the first three weeks after the operation, the maximum lactate dehydrogenase (LDH) was up to 7,430 U/l and the minimum hemoglobin was 4.9 g/dl. The mitral valve replacement successfully resolved the hemolysis, but the renal function did not completely recover.


Subject(s)
Anemia, Hemolytic/physiopathology , Mitral Valve Insufficiency/surgery , Mitral Valve/surgery , Postoperative Complications/physiopathology , Anemia, Hemolytic/diagnosis , Anemia, Hemolytic/enzymology , Anemia, Hemolytic/etiology , Echocardiography, Transesophageal , Hematocrit , Humans , L-Lactate Dehydrogenase/blood , Male , Middle Aged , Reoperation
8.
J Cardiol ; 45(2): 69-73, 2005 Feb.
Article in Japanese | MEDLINE | ID: mdl-15768609

ABSTRACT

An 83-year-old woman was transferred to our hospital because of pacing failure and suspected ventricular perforation by a permanent pacing lead. She had undergone permanent pacemaker implantation 5 months previously. Chest radiography showed the pacing lead running out of the cardiac shadow. Computed tomography and echocardiography confirmed the diagnosis of ventricular perforation by the pacing lead. No evidence of cardiac tamponade was found. The lead was surgically removed through a median sternotomy. Intraoperatively, the lead was found perforating the ventricle and the pericardium, and reaching into the left pleural cavity but not injuring the left lung. A pacing lead may potentially injure the heart or the lung. Regular check-up of lead position and pacing status is recommended.


Subject(s)
Device Removal , Heart Rupture/surgery , Pacemaker, Artificial/adverse effects , Pericardium , Ventricular Septal Rupture/surgery , Aged , Aged, 80 and over , Echocardiography , Female , Heart Rupture/etiology , Humans , Pericardium/diagnostic imaging , Ventricular Septal Rupture/etiology
9.
Ann Thorac Cardiovasc Surg ; 11(6): 416-8, 2005 Dec.
Article in English | MEDLINE | ID: mdl-16401994

ABSTRACT

A 61-year-old man presented with consistent hemolytic anemia 15 months after ascending and total arch replacement for DeBakey I type acute aortic dissection. The cause of hemolysis turned out to be mechanical damage of red blood cells at the inverted felt of the proximal anastomosis. Reoperation of resection of the felt and repair of the proximal anastomosis successfully resolved this problem. We report a rare case of hemolytic anemia at the site of inverted felt strip after total arch replacement.


Subject(s)
Anemia, Hemolytic/etiology , Aortic Aneurysm, Thoracic/surgery , Aortic Aneurysm/surgery , Aortic Dissection/surgery , Humans , Male , Middle Aged , Postoperative Complications , Reoperation
10.
J Radiat Res ; 44(3): 271-6, 2003 Sep.
Article in English | MEDLINE | ID: mdl-14646232

ABSTRACT

The effects of heavy ion particle irradiation on meiosis and reproductive development in the nematode Caenorhabditis elegans were studied. Meiotic pachytene nuclei are significantly resistant to particle irradiation by the heavy ions carbon and argon, as well as to X-rays, but not UV, whereas diplotene to diakinesis stage oocytes and early embryonic cells are not. Chromosomal abnormalities appear in mitotic cells and in maturing oocytes irradiated with heavy ion particles during the diplotene to the early diakinesis stages, but not in oocytes irradiated during the pachytene stage. The pachytene nuclei of ced-3 mutants, which are defective in apoptosis, are similarly resistant to ionizing radiation, but pachytene nuclei depleted for Ce-atl-1 (ataxia-telangiectasia like 1) or Ce-rdh-1/rad-51 are more sensitive. Pachytene nuclei thus appear to effectively repair heavy ion-induced DNA damage by the meiotic homologous recombination system.


Subject(s)
Caenorhabditis elegans/radiation effects , DNA Damage/genetics , DNA Repair/physiology , DNA/radiation effects , Heavy Ions , Oogenesis/radiation effects , Prophase/genetics , Prophase/radiation effects , Animals , Ataxia Telangiectasia Mutated Proteins , Caenorhabditis elegans/genetics , Caenorhabditis elegans/metabolism , Caenorhabditis elegans Proteins , Dose-Response Relationship, Radiation , Meiosis/genetics , Meiosis/radiation effects , Phosphotransferases/deficiency , Radiation Dosage , Radiation Tolerance/genetics , Recombination, Genetic/radiation effects , Ultraviolet Rays , X-Rays
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