ABSTRACT
Bovine tuberculosis (BTB) is a zoonotic disease caused by Mycobacterium bovis, a member of the Mycobacterium tuberculosis complex (MTC). The quick and specific detection of this species is of extreme importance, since BTB may cause economic impacts, in addition to presenting imminent risks to human health. In the present study a nested real-time PCR test (nested q-PCR) was used in post-mortem evaluations to assess cattle carcasses with BTB-suspected lesions. A total of 41,193 cattle slaughtered in slaughterhouses located in the state of Mato Grosso, were examined. Of the examined animals, 198 (0.48%) showed BTB-suspected lesions. M. bovis was isolated in 1.5% (3/198) of the samples. Multiplex-PCR detected MTC in 7% (14/198) of the samples. The nested q-PCR test detected MTC in 28% (56/198) of the BTB-suspected lesions, demonstrating higher efficiency when compared to the multiplex-PCR and conventional microbiology. Nested q-PCR can therefore be used as a complementary test in the national program for control and eradication of bovine tuberculosis.
Subject(s)
DNA, Bacterial/analysis , Food Inspection/methods , Meat/microbiology , Molecular Typing/veterinary , Mycobacterium bovis/isolation & purification , Tuberculosis, Bovine/microbiology , Tuberculosis, Lymph Node/veterinary , Abattoirs , Animals , Brazil , Cattle , DNA, Bacterial/isolation & purification , DNA, Bacterial/metabolism , Efficiency , Head , Limit of Detection , Lung/chemistry , Lung/microbiology , Lymph Nodes/chemistry , Lymph Nodes/microbiology , Meat/analysis , Molecular Typing/methods , Multiplex Polymerase Chain Reaction/veterinary , Mycobacterium bovis/classification , Neck , Real-Time Polymerase Chain Reaction/veterinary , Reverse Transcriptase Polymerase Chain Reaction/veterinary , Thorax , Tuberculosis, Bovine/physiopathology , Tuberculosis, Bovine/prevention & control , Tuberculosis, Lymph Node/etiologyABSTRACT
The presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6 percent) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5 percent) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.
Subject(s)
Cattle , Animals , Abattoirs , Bacterial Typing Techniques/methods , Meat/microbiology , Mycobacterium bovis/isolation & purification , Tuberculosis, Bovine/microbiology , Brazil , DNA, Bacterial , Genotype , Mycobacterium bovis/classification , Mycobacterium bovis/genetics , Polymerase Chain Reaction , Restriction MappingABSTRACT
The presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.
Subject(s)
Bacterial Typing Techniques/methods , Meat/microbiology , Mycobacterium bovis/isolation & purification , Tuberculosis, Bovine/microbiology , Abattoirs , Animals , Brazil , Cattle , DNA, Bacterial/analysis , Genotype , Mycobacterium bovis/classification , Mycobacterium bovis/genetics , Polymerase Chain Reaction , Restriction MappingABSTRACT
Amostras de soro obtidas de estudantes do curso de Medicina Veterinária da Universidade para o desenvolvimento do Estado e da Regiäo do Pantanal, Campo Grande, MS, Brasil, foram examinadas para a presença de anticorpos contra Toxoplasma gondii. Dos 145 soros testados, 44 (30,34 por cento) foram positivos na hemaglutinaçäo, com título igual ou superior a 1:16. Näo foram observadas associaçöes entre as características epidemiológicas examinadas tais como hábitos alimentares (ingestäo de carne bovina crua ou malpassada, vegetais crus/näo lavados, produtos lácteos näo pasteurizados) ou contato constante com cäes e a presença de anticorpos contra T. gondii, exceto pelo percentual significativamente maior de estudantes soropositivos que relataram ter contato freqüente com gatos (P=0,03).
