Effects of Probiotic-Enriched Minas Cheese (Lactobacillus acidophilus La-05) on Cardiovascular Parameters in 5/6 Nephrectomized Rats.

Dairy foods have become an interest in chronic kidney disease (CKD) due to their nutritional profile, which makes them a good substrate for probiotics incorporation. This study evaluated the effect of probiotic-enriched Minas cheese with Lactobacillus acidophilus La-05 in an experimental rat model for CKD on cardiac, inflammatory, and oxidative stress parameters. Male Wistar rats were divided into 4 groups (n = 7/group): 5/6 nephrectomy + conventional Minas cheese (NxC); 5/6 nephrectomy + probiotic Minas cheese (NxPC); Sham + conventional Minas cheese (ShamC); Sham + probiotic Minas cheese (ShamPC). Offering 20 g/day of Minas cheese with Lact. acidophilus La-05 (108-109 log CFU/g) for 6 weeks. The cardiomyocyte diameter was determined. Superoxide dismutase (SOD) activity in plasma, heart, kidney, and colon tissue was performed. At the end of supplementation, no significant changes in lipid profile and renal parameters were found. The NxPC group showed a decrease in cardiomyocyte diameter compared to the NxC group (16.99 ± 0.85 vs. 19.05 ± 0.56 µm, p = 0.0162); also they showed reduced plasmatic SOD activity (502.8 ± 49.12 vs. 599.4 ± 94.69 U/mL, p < 0.0001). In summary, probiotic-enriched Minas cheese (Lact. acidophilus La-05) consumption suggests a promisor cardioprotective effect and was able to downregulate SOD activity in a rat model of CKD.

MULT: A new BMI reference to assess nutritional status of multi-ethnic children and adolescents.

OBJECTIVES: To develop a new Body Mass Index (BMI) reference (MULT) based on longitudinal data of multi-ethnic populations and to compare it to international BMI references. METHODS: The MULT BMI reference was constructed through the LMS method and the Generalized Additive Models for Location Scale and Shape (GAMLSS), with 81 310 observations of 17 505 subjects aged 0-22 years old, from the United Kingdom, Ethiopia, Peru, India, Vietnam, Brazil, and Portugal. Outlier values were removed based on weight z-scores (population level) and based on BMI z-scores using the linear mixed effects model (individual level). The MULT M, S and L curves were compared to the ones of the World Health Organization (WHO), Centers for Disease Control and Prevention (CDC), International Obesity Task Force (IOTF), and Dutch Growth Study (DUTCH). The MULT BMI percentile cutoffs for overweight and obesity were calculated using the adult BMI values of 25 and 30 kg/m2 at 17, 18, 19, and 20 years old. RESULTS: MULT presented the lowest mean BMI values for the ages 102-240 months for boys and 114-220 months for girls. MULT S values were similar to the WHO and IOTF for children under 60 months of age and the highest during puberty, while the L curve showed to be more symmetric than the other BMI references. CONCLUSION: The MULT BMI reference was constructed based on recent data of populations from 10 countries, being a good option to assess the nutritional status of multi-ethnic populations.

MULT: New height references and their efficiency in multi-ethnic populations.

OBJECTIVES: To develop new height references (MULT) based on longitudinal data of multi-ethnic populations and to compare them to the height references from the Dutch Growth Study, from the Centers for Disease Control and Prevention (CDC) and from the World Health Organization (WHO). METHODS: The MUL height references were developed through the LMS method and the Generalized Additive Models for Location Scale and Shape. They were constructed based on 2611 subjects (15 292 measurements) from the advantaged quintile of the Young Lives (Younger Cohort), Millennium Cohort Study, Adolescent Nutritional Assessment Longitudinal Study, and Epidemiological Health Investigation of Teenagers in Porto studies. The M, S curves were described to compare the growth trajectory of the MULT, DUTCH, CDC and WHO height references. For the population comparative analysis, we used the total sample of the studies (91 063 observations, 17 641 subjects). The Lin's concordance correlation coefficient (CCC) and Cohen's kappa coefficient (K) were used to verify the agreement between MULT, WHO and CDC height references. RESULTS: The MULT height references showed taller boys for the periods of 61-174 months and 196-240 months and taller girls for 61-147 and 181-240 months, when compared to CDC and WHO height references. There was an almost perfect agreement between WHO and MULT height references (CCC >0.99) for the subjects aged 2 to 5 years. CONCLUSIONS: MULT height references presented a taller population and a high agreement with WHO growth charts, especially for children under 5 years, indicating that it could be useful to assess nutritional status of multi-ethnic populations.

Extracellular Vesicles Regulate Biofilm Formation and Yeast-to-Hypha Differentiation in Candida albicans.

In this study, we investigated the influence of fungal extracellular vesicles (EVs) during biofilm formation and morphogenesis in Candida albicans. Using crystal violet staining and scanning electron microscopy (SEM), we demonstrated that C. albicans EVs inhibited biofilm formation in vitro. By time-lapse microscopy and SEM, we showed that C. albicans EV treatment stopped filamentation and promoted pseudohyphae formation with multiple budding sites. The ability of C. albicans EVs to regulate dimorphism was further compared to EVs isolated from different C. albicans strains, Saccharomyces cerevisiae, and Histoplasma capsulatum. C. albicans EVs from distinct strains inhibited yeast-to-hyphae differentiation with morphological changes occurring in less than 4 h. EVs from S. cerevisiae and H. capsulatum modestly reduced morphogenesis, and the effect was evident after 24 h of incubation. The inhibitory activity of C. albicans EVs on phase transition was promoted by a combination of lipid compounds, which were identified by gas chromatography-tandem mass spectrometry analysis as sesquiterpenes, diterpenes, and fatty acids. Remarkably, C. albicans EVs were also able to reverse filamentation. Finally, C. albicans cells treated with C. albicans EVs for 24 h lost their capacity to penetrate agar and were avirulent when inoculated into Galleria mellonella. Our results indicate that fungal EVs can regulate yeast-to-hypha differentiation, thereby inhibiting biofilm formation and attenuating virulence. IMPORTANCE The ability to undergo morphological changes during adaptation to distinct environments is exploited by Candida albicans and has a direct impact on biofilm formation and virulence. Morphogenesis is controlled by a diversity of stimuli, including osmotic stress, pH, starvation, presence of serum, and microbial components, among others. Apart from external inducers, C. albicans also produces autoregulatory substances. Farnesol and tyrosol are examples of quorum-sensing molecules (QSM) released by C. albicans to regulate yeast-to-hypha conversion. Here, we demonstrate that fungal EVs are messengers impacting biofilm formation, morphogenesis, and virulence in C. albicans. The major players exported in C. albicans EVs included sesquiterpenes, diterpenes, and fatty acids. The understanding of how C. albicans cells communicate to regulate physiology and pathogenesis can lead to novel therapeutic tools to combat candidiasis.

Rapid Ethnonutrition Assessment Method Is Useful to Prototype Dietary Assessments with a Focus on Local Biodiverse Food Plants.

Ethnonutrition is the study of diets in the context of food systems of different peoples and cultures. Its scope comprises native or local categories used to classify food, and also includes biodiverse food availability, local culinary techniques, seasonality, and cultural perceptions related to diet with nutritional implications. Here, we describe a method useful for gathering ethnonutrition data to design dietary interventions or assessments, the Rapid Ethnonutrition Assessment (REA). REA is a tool that offers food and nutrition research a broad biocultural view of diets, considering food system-level, by prototyping dietary assessments with high efficiency. This method permits us to prevent misinterpretations that lead to wrong conclusions in nutritional research.

Inflammatory pain assessment in the arthritis of the temporomandibular joint in rats: A comparison between two phlogistic agents.

Temporomandibular joint (TMJ) disorders are a group of conditions that result in TMJ pain, which frequently limits basic daily activities. Experimental models that allow the study of the mechanisms underlying these inflammatory and pain conditions are of great clinical relevance. The aim of this study was to evaluate nociception, inflammation and participation of the macrophage/microglia cells in the arthritis of the TMJ induced by two phlogistic agents. 84 rats were divided into 2 groups: Zy, which received zymosan intra-articularly, or Cg, which received carrageenan intra-articularly. Mechanical nociception, total leukocyte influx to the synovial fluid and histopathological analyses were evaluated in the TMJ. The participation of macrophage/microglia located in trigeminal ganglia (TG) and in the subnucleus caudalis (V-SnC) was assessed immunohistochemically. Both agents induced mechanical hyperalgesia 6h after the induction, but a more persistent algesic state was perceived in the Cg group, which lasted for 120h. Even though both groups presented increased leukocyte influx, the Zy-group presented a more intense influx. Zymosan recruited resident macrophage in the trigeminal ganglia 24h after the injection. In the V-SnC, the group Cg presented a more prolonged immunolabeling pattern in comparison with the group Zy. It can be concluded that zymosan induced a more intense infiltrate and peripheral nervous changes, while Cg lead to a moderate TMJ inflammation with prominent changes in the V-SnC.

Antinociceptive and anti-inflammatory effects of Caryocar coriaceum Wittm fruit pulp fixed ethyl acetate extract on zymosan-induced arthritis in rats.

The ethyl acetate extract from the fruit pulp of Caryocar coriaceum Wittm (Caryocaraceae), popularly known as pequi, has wide applications in popular medicine. Preclinical tests have demonstrated the therapeutic properties of the oil. We investigated the antinociceptive and anti-inflammatory effects of Pequi C. coriaceum Wittm ethyl acetate extract (PCCO) on zymosan-induced arthritis in rat knee joint. The animals were pretreated with PCCO for 7 consecutive days or with a single dose. Paw elevation time (PET), leukocyte infiltration, myeloperoxidase activity (MPO) and cytokine levels were assessed 4h after zymosan injection. Synovial tissue was harvested for immunohistochemical analysis, edema and vascular permeability. We observed a significant decrease in PET with PCCO pretreatment. PCCO showed a significant reduction of leukocyte migration and a decrease in MPO. Decreases were observed in cytokine release in the synovial fluid and TNF-α and cyclooxygenase-1 immunostaining in synovial tissue. Edema was inhibited by treatment with all doses of PCCO. The data suggest that PCCO exerts antinociceptive and anti-inflammatory effects on arthritis in rats.

Characteristics associated with media use in early adolescence.

This study aimed to identify socioeconomic and behavioral characteristics associated with media use in early adolescence. The sample was comprised of 1,680 adolescents (884 girls and 796 boys) aged 13, attending private and public schools in Porto, Portugal, during 2003/2004. Adolescents completed questionnaires pertaining to demographic, social and behavioral characteristics, including the time spent watching television and playing computer games on week and weekend days. Logistic and proportional ordinal regressions showed that attending public schools, sleeping less time, using tobacco and presenting low levels of physical activity were factors associated with high media use. In boys living with one of their parents as well as living with younger and older parents were also associated with high media use. Besides the association with less healthier behaviours, we also found higher media use among adolescents from lower social classes and less structured families, which may increase their exposure to negative influence of the media.

Characteristics associated with media use in early adolescence / Características associadas com o uso da mídia no início da adolescência / Características asociadas con el uso de medios de comunicación en la adolescencia temprana

This study aimed to identify socioeconomic and behavioral characteristics associated with media use in early adolescence. The sample was comprised of 1,680 adolescents (884 girls and 796 boys) aged 13, attending private and public schools in Porto, Portugal, during 2003/2004. Adolescents completed questionnaires pertaining to demographic, social and behavioral characteristics, including the time spent watching television and playing computer games on week and weekend days. Logistic and proportional ordinal regressions showed that attending public schools, sleeping less time, using tobacco and presenting low levels of physical activity were factors associated with high media use. In boys living with one of their parents as well as living with younger and older parents were also associated with high media use. Besides the association with less healthier behaviours, we also found higher media use among adolescents from lower social classes and less structured families, which may increase their exposure to negative influence of the media.

O objetivo do estudo foi identificar as características socioeconômicas e comportamentais associadas ao uso da mídia, no início da adolescência. A amostra foi constituída por 1.680 adolescentes (884 garotas e 796 garotos) de 13 anos que frequentavam escolas públicas e privadas no Porto, Portugal, em 2003/2004. Eles preencheram questionários com informação sobre características sociodemográficas e comportamentais, incluindo tempo gasto com o uso da televisão e computador, em dias da semana e fins de semana. Regressões logísticas ordinais e proporcionais mostraram que os adolescentes das escolas públicas, os que dormiam menos, consumiam tabaco e praticavam pouca atividade física tinham maior propensão a usar a mídia mais tempo. Garotos que viviam com um dos pais ou que tinham pais mais jovens ou mais velhos usavam mais tempo a mídia. Assim, além da associação com comportamentos menos saudáveis, constatou-se um maior uso da mídia nos adolescentes de classe social inferior e de famílias menos estruturadas, o que pode aumentar a sua permeabilidade à influência negativa da mídia.

El objetivo del estudio fue identificar las características socioeconómicas y de comportamiento asociadas con el uso de medios de comunicación en la adolescencia temprana. La muestra se constituyó por 1.680 adolescentes (884 niñas y 796 niños) de 13 años de escuelas públicas y privadas en Porto, Portugal, en 2003/2004. Estos completaron cuestionarios que contenían características sociodemográficas y de comportamiento, incluyendo el tiempo empleado en ver la televisión y el ordenador, en días de semana y fines de semana. Regresiones logísticas ordinales y proporcionales mostraron que los adolescentes de las escuelas públicas, los que dormían menos, consumían tabaco y practican poca actividad física eran más propensos a usar más medios de comunicación. Los niños que viven con un solo padre o con padres más jóvenes o más viejos los utilizaban también más. Así, además de la asociación con comportamientos poco saludables, se concluyó que un mayor uso de los medios de comunicación en los adolescentes de familias de clase social baja y de familias menos estructuradas, lo que puede aumentar su permeabilidad a la influencia negativa de estos medios.

Role of NMDA receptors in the trigeminal pathway, and the modulatory effect of magnesium in a model of rat temporomandibular joint arthritis.

Temporomandibular joint (TMJ) arthritis is a common cause of orofacial pain. In the present study, the modulatory effects of N-methyl-d-aspartate receptors (NMDA-Rs) and magnesium were investigated in TMJ arthritis hypernociception. Male Wistar rats received an intra-articular injection of carrageenan (Cg) in the TMJ, and mechanical hypernociception was measured. The NMDA-R antagonist, MK-801, and magnesium chloride (MgCl2 ) were administered before arthritis induction. Magnesium deficiency was promoted by feeding rats a synthetic magnesium-free diet for 9 d before injection of Cg. The Cg induced mechanical hypernociception that lasted for 120 h. MK-801 inhibited this hypernociceptive state. MgCl2 pretreatment prevented Cg-induced hypernociception and altered the nociceptive threshold in the absence of Cg. Magnesium deficiency increased hypernociception and induced spontaneous hypernociceptive behavior. TMJ arthritis increased the expression of mRNA for all NMDA-R subunits and immunostaining of phosphorylated NR1 (phospho-NR1). MgCl2 inhibited expression of NR2B mRNA and phospho-NR1 immunostaining and increased expression of NR3 mRNA. Magnesium deficiency increased expression of both NR1 and NR3 mRNAs and phospho-NR1 immunostaining in the trigeminal subnucleus caudalis. We found that magnesium modulates nociceptive behavior and induces NMDA-R subunit rearrangement in the subnucleus caudalis. The present results may lead to a better understanding of central processing in the nociceptive trigeminal pathway and the development of new approaches to treat orofacial pain with a TMJ origin.

CB1 and CB2 contribute to antinociceptive and anti-inflammatory effects of electroacupuncture on experimental arthritis of the rat temporomandibular joint.

Electroacupuncture (EA) and cannabinoids have been reported to have anti-inflammatory and antinociceptive effects in animal models of arthritis. Male Wistar rats were injected with saline or zymosan (2 mg) into the temporomandibular joint (TMJ). EA (10 Hz, 30 min) was performed 2 h after or 1 h before zymosan administration. AM251 or AM630 (3 mg/kg, i.p.)were administered before EA treatment. Mechanical hypernociception was accessed after zymosan administration. Rats were sacrificed 6 h after zymosan administration and the joint was removed for histopathological analysis. The gene expression of CB1 and CB2 receptors was assessed after sacrifice of the TMJ arthritic animals. EA inhibited zymosan-induced hypernociception (p < 0.05). AM251 reversed significantly the antinociceptive effect of EA, suggesting that the CB1 receptor is involved in this effect. AM630 reversed the anti-inflammatory effect of EA. CB1 and CB2 receptor gene expression was upregulated 6 h after zymosan-induced arthritis in the EA-treated group. We observed downregulation of CB2 receptor gene expression in the EA group at the 24th hour compared with the 6th hour. Higher CB1 receptor gene expression was also found compared with the 6th hour. EA produced antinociceptive and anti-inflammatory effects, and these effects appeared to be mediated through CB1 and CB2 receptor activation.

Divisão e expansão da crista do rebordo em duas etapas para instalação de implantes na região mandibular posterior / A two-step, split crest technique at posterior mandible for implant placement - case report

A reabilitação da região posterior da mandíbula atrófica com implantes osseointegráveis é um grande desafio. Os padrões de reabsorção pós-exodontia modificam significativamente a largura e a altura do rebordo ósseo residual. Diferentes técnicas já foram propostas para o tratamento de mandíbulas severamente reabsorvidas. A técnica de divisão e expansão da crista na mandíbula não é controlável devido a grande espessura das corticais ósseas, causando o desprendimento total do bloco ósseo. Uma abordagem técnica em duas etapas na mandíbula é indicada para evitar complicações, diminuindo-se assim à taxa de insucesso sem, no entanto, causar um atraso significante no tratamento.

Rehabilitation of atrophic, mandibular posterior regions with dental implants is a significant challenge. Post-extraction tooth resorption patterns can significantly modify the width and height of the residual bone. Several techniques have been proposed for the treatment of severely resorbed jaws. The technique of crest division and expansion cannot be predictable due to the large thickness of the cortical bone, causing total detachment of bone blocks. Thus, a two-step technical approach may be indicated to prevent complications, decreasing the morbidity rate without, however, causing a significant delaying on treatment time