ABSTRACT
A Japanese hydrangea phyllody (JHP) disease found throughout Japan causes economic damage to the horticultural industry. JHP phytoplasma-infected Japanese hydrangea plants show several disease symptoms involved in floral malformations, such as virescence, phyllody and proliferation. Here, we cloned and characterized the antigenic membrane protein (Amp) gene homolog from the JHP phytoplasma (JHP-amp), expressed the JHP-Amp protein in Escherichia coli cells, and then obtained an antibody against JHP-Amp. The antibody against JHP-Amp had no cross-reactions with the antibody against the Amp protein from a closely related onion yellows phytoplasma. This serologic specificity is probably due to the high diversity of the hydrophilic domains in the Amp proteins. The in situ detection of the JHP-Amp protein revealed that the JHP phytoplasma was localized to the phloem tissues in the malformed flower. This study shows that the JHP-Amp protein is indeed a membrane protein, which is expressed at detectable level in the JHP phytoplasma-infected hydrangea.
Subject(s)
Bacterial Proteins/metabolism , Flowers/microbiology , Hydrangea/microbiology , Membrane Proteins/metabolism , Phytoplasma/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/immunology , Blotting, Western , Cloning, Molecular , Electrophoresis, Polyacrylamide Gel , Escherichia coli/genetics , Japan , Membrane Proteins/genetics , Membrane Proteins/immunology , Phloem/microbiology , Phytoplasma/genetics , Plant Diseases/microbiology , Recombinant Proteins/metabolismABSTRACT
Révész (1934) reported that haptic illusions were observed in almost all of the geometrical optical illusion figures. The present study reexamined seven geometrical illusions in both haptic and visual modes. In the Müller-Lyer, Ponzo, and vertical-horizontal figures, haptic illusions equivalent to the visual illusions were observed. In the Oppel-Kundt figure, a haptic illusion similar to the visual one was obtained. In the haptic Delboeuf stimuli, the size illusion of the outer circle occurred, whereas that of the inner circle did not. No haptic illusion was obtained in the Poggendorff figure. In the Zöllner figure, a haptic illusion directionally opposite to the visual one was obtained. These results show that haptic illusions do not occur in all of the geometrical illusion figures. They also suggest that haptic illusions are not necessarily mediated by visualization and that haptic processing of the figures often occurs in a manner different from vision.