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2.
J Glob Antimicrob Resist ; 10: 261-263, 2017 09.
Article in English | MEDLINE | ID: mdl-28732791

ABSTRACT

INTRODUCTION: Bovine mastitis causes important economic losses in the dairy industry. Coagulase-negative staphylococci (CNS) are a group of bacteria commonly isolated from bovine mastitis and can display resistance to a wide range of antimicrobial agents. OBJECTIVES: The objective of this study was to determine staphylococcal resistance towards ß-lactam, macrolide and lincosamide antimicrobials in quarters previously treated with third-generation cephalosporin and after lincosamide intramammary therapy. METHODS: Sick quarters of eighteen cows from Villaguay, Entre Ríos (Argentina) with clinical mastitis were studied. All staphylococcal isolates were tested by disk diffusion for their antimicrobial susceptibilities. Cefoxitin resistance was investigated by PCR and sequencing for both the mecA and mecC genes. RESULTS: Resistances to penicillin, oxacillin and cefoxitin were observed, whereas no resistance to macrolide and lincosamide was detected. A cefoxitin-resistant Staphylococcus saprophyticus was found to be mecA-negative but mecC-positive. CONCLUSIONS: This study reports for the first time the mecC gene from a CNS in bovine mastitis in South America. Because CNS may act as reservoirs of antimicrobial resistance genes, they can be seen as a potential public health threat with respect to antimicrobial resistance and the development of multiple resistance. Also, the emergence of methicillin-resistant phenotypes will limit therapeutic options.


Subject(s)
Anti-Bacterial Agents/pharmacology , Bacterial Proteins/genetics , Drug Resistance, Bacterial , Mastitis, Bovine/microbiology , Staphylococcal Infections/veterinary , Staphylococcus saprophyticus/isolation & purification , Animals , Argentina , Cattle , Cefoxitin/pharmacology , Female , Lincosamides/pharmacology , Macrolides/pharmacology , Microbial Sensitivity Tests , Sequence Analysis, DNA , Staphylococcus saprophyticus/drug effects , Staphylococcus saprophyticus/genetics , beta-Lactams/pharmacology
3.
FEMS Microbiol Lett ; 364(8)2017 04 01.
Article in English | MEDLINE | ID: mdl-28087612

ABSTRACT

Mastitis affects the health and welfare of dairy cows worldwide. Coagulase-negative staphylococci (CNS) are known to form biofilms and are increasingly recognized as a cause of persistent bovine intramammary infections. A total of 90 CNS isolated from cows with clinical and subclinical mastitis in Argentina from 2008 to 2014 were identified by PCR-RFLP using the gap gene. Standard microtiter plate assays were used to assess CNS biofilm formation, and Staphylococcus haemolyticus species formed the strongest biofilms. The presence of biofilm-associated genes icaA, bap and aap was detected in a few isolates, while embP, fbe, atlE and eno were present in the majority of isolates. Genes encoding resistance to ß-lactams were detected among the isolates; blaZ, mecA and mecC were detected in 21, 4 and 1 isolate, respectively. Resistance to macrolides and lincosamides (n = 6) was attributable to ermB, ermC, mphC or mrsA or a combination of those genes. In this study, we identified CNS species involved in mastitis and provide information about pathogenicity and antimicrobial resistance, which is essential to design efficient strategies to control mastitis caused by CNS.


Subject(s)
Anti-Bacterial Agents/pharmacology , Biofilms/growth & development , Drug Resistance, Multiple, Bacterial/genetics , Mastitis, Bovine/microbiology , Staphylococcal Infections/veterinary , Staphylococcus/physiology , Animals , Argentina , Cattle , Coagulase/genetics , Coagulase/metabolism , Female , Genes, Bacterial , Microbial Sensitivity Tests , Polymorphism, Restriction Fragment Length , Real-Time Polymerase Chain Reaction , Staphylococcal Infections/microbiology , Staphylococcus/drug effects , Staphylococcus/genetics , Staphylococcus/isolation & purification
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