ABSTRACT
1. Ethionine administered acutely to the adult female rat markedly elevates and then lowers plasma iron concentration over several days. Liver iron undergoes a reverse cycle. 2. Ethionine does not cause changes in the blood parameters, including total plasma iron-binding capacity and plasma iron clearance. Erythrocytes of rats injected with ethionine show altered responses to hypertonicity. 3. Increased reticulo-endothelial activity of the spleen, indicated by increased uptake of 59Fe-labelled erythrocytes by liver and spleen, apparently contributes to plasma iron elevation. Also the liver releases iron which further raises plasma iron.
Subject(s)
Ethionine/pharmacology , Iron/metabolism , Animals , Erythrocytes/drug effects , Erythrocytes/metabolism , Ethionine/antagonists & inhibitors , Female , Iron/blood , Liver/drug effects , Liver/metabolism , Osmotic Fragility/drug effects , Rats , Spleen/drug effects , Spleen/metabolism , Time Factors , Transferrin/metabolismSubject(s)
Carrier Proteins/isolation & purification , Iron/metabolism , Reticulocytes/analysis , Animals , Butanols/pharmacology , Carrier Proteins/metabolism , Chromatography, Gel , Cytoplasm/metabolism , Deoxycholic Acid/pharmacology , Erythrocytes/analysis , Iodine Isotopes , Iron Isotopes , Kinetics , Molecular Weight , Protein Binding , Rabbits , Reticulocytes/cytology , Reticulocytes/drug effects , Reticulocytes/metabolism , Sodium Dodecyl Sulfate/pharmacology , Solubility , Subcellular Fractions/metabolism , Surface-Active Agents/pharmacology , Transferrin/metabolismSubject(s)
Iron/metabolism , Placenta/metabolism , Animals , Binding Sites , Female , Maternal-Fetal Exchange , Pregnancy , Rats , Transferrin/metabolism , Vitelline Membrane/metabolismSubject(s)
Maternal-Fetal Exchange , Mercury/metabolism , Placenta/metabolism , Animals , Arsenic/pharmacology , Citrates/pharmacology , Extraembryonic Membranes/metabolism , Female , Fetus/metabolism , Mercury Isotopes , Methods , Placenta/analysis , Placenta/drug effects , Pregnancy , Rats , Rats, Inbred Strains , Sodium/pharmacologyABSTRACT
Reticulocytosis was induced in rabbits with phenylhydrazine. The accumulation of a small part of (59)Fe in blood cells of these animals was inhibited by ouabain and related to changes in extracellular sodium and potassium concentrations. Sodium increases movement from the cell surface into the cell, whereas potassium and ouabain decrease this movement. (59)Fe movement was found to be temperature-dependent. Thus, the Na-K ATPase system appears to be important in the movement of iron from the cell membrane (stroma) to the cell interior, but influences only a small part of the total iron transport.