ABSTRACT
Guanine-rich sequences of DNA can form quadruply-stranded structures. It has been shown that folding single-stranded telomeric DNA into a quadruplex structure inhibits telomerase (an enzyme overexpressed in 85-90% of cancer cells). On the other hand, it has been hypothesised that the formation of quadruplex DNA structures in the promoter region of some oncogenes plays an important role in regulating the transcription of the corresponding gene. Consequently, there is great current interest in developing small molecules that can bind selectively to quadruplex DNA and in doing so could act as anticancer drugs. This review aims to discuss the different types of ligands that have been recently developed as quadruplex DNA stabilisers. The review is organised by the type of compound and mainly covers the literature between 2004 and 2007.
Subject(s)
Antineoplastic Agents/pharmacology , DNA/chemistry , Enzyme Inhibitors/pharmacology , G-Quadruplexes/drug effects , Animals , Antineoplastic Agents/chemistry , DNA/metabolism , Enzyme Inhibitors/chemistry , Gene Expression Regulation/drug effects , Guanine/chemistry , Guanine/metabolism , Humans , Proto-Oncogene Proteins/chemistry , Proto-Oncogene Proteins/genetics , Proto-Oncogene Proteins/metabolism , Telomerase/genetics , Telomerase/metabolismABSTRACT
MOTIVATION: AutoGrid/AutoDock is one of the most popular software packages for docking, but its automation is not trivial for tasks such as (1) the virtual screening of a library of ligands against a set of possible receptors; (2) the use of receptor flexibility and (3) making a blind-docking experiment with the whole receptor surface. This is an obstacle for research teams in the fields of Chemistry and the Life Sciences who are interested in conducting this kind of experiment but do not have enough programming skills. To overcome these limitations, we have designed BDT, an easy-to-use graphic interface for AutoGrid/AutoDock. AVAILABILITY: BDT is available for free, upon request, for non-commercial research.
