ABSTRACT
The in vitro influence of external electrostatic fields with 200 kV/m tension on the biophysical parameters of the erythrocyte membranes and their ghosts of white outbred rats was studied. The investigation on the parameters of erythrocyte membranes and their ghosts, particularly, their microviscosity, the amount and degree of membrane proteins submersion in lipids, polarity in depth of the membrane bilayer and its viscositywas carried out by the spectrofluorimeteric method using pyrene as a hydrophobic fluorescent probe. The analyses of literature data, findings of the current study and their comparison with the results of our previous works allow of concluding that the in vitro influence of external electrostatic fields with 200 kV/m tension on the erythrocyte membranes and their ghosts occurs at different sites of membrane. It is shown that the preliminary exposure of erythrocytes in external electrostatic fields leads to the changes of the parameters both of a membrane surface layer and the intra-membrane domains. So, the decrease in the strength of peripheral proteins binding to the erythrocyte membranes and the increase in the micriviscosity of the lipid bilayer are observed. The influence of the field on the ghosts of intact erythrocytes results in alterations of the studied parameters only of the membrane surface.
Subject(s)
Erythrocyte Membrane/chemistry , Lipid Bilayers/chemistry , Membrane Lipids/chemistry , Animals , Electromagnetic Fields , Erythrocytes/chemistry , In Vitro Techniques , Male , Membrane Proteins/chemistry , Rats , Static Electricity , ViscosityABSTRACT
Experimental rats were exposed to an electrostatic field of 200 kW/m for 1, 24 or 6 hours a day for 6 days. The effect of this exposure on the structure and function of adrenal chromaffin bodies and the concentration of catecholamines and their precursors in blood and adrenals was investigated. Using histochemical, fluorescent-microscopic and spectrofluorimetric methods, it was found that the exposure may modify the structure and function of adrenal chromaffins and the concentration of catecholamines in blood and adrenals. These changes depended on the exposure time. An hour exposure activated the secretory apparatus of adrenal chromaffins and enhanced catecholamine secretion to blood. A 24-hour exposure led to an increase in the concentration of catecholamines in adrenals and blood and a change in the organ cytoangioarchitectonics. A 6-day fractionated exposure resulted in a decrease of catecholamines both in blood and adrenals.
Subject(s)
Adrenal Glands/metabolism , Catecholamines/metabolism , Electricity/adverse effects , Animals , Chromaffin System/metabolism , Dihydroxyphenylalanine/metabolism , Dopamine/metabolism , Epinephrine/metabolism , Fluorescence , Male , Norepinephrine/metabolism , Rats , Spectrometry, FluorescenceABSTRACT
Action of the external electrostatic field on electrophysical features of DNA dry fibres was studied. It is shown that an increase of DNA electric conductivity begins from some threshold voltage mean of the external field. On the basis of the suggested DNA semiconductive properties it was concluded that the observed effect was the consequence of Frenkel's thermoelectronic ionisation.
Subject(s)
DNA , Electricity , Animals , Cattle , Electric Conductivity , Spleen/analysisABSTRACT
Rats with experimental hypoparathyrosis showed an increase in the content of Ca2+ in liver and brain mitochondria, discovered by fluorescent testing with chlorotetracycline. That increase correlated with the degree of hypofunction of the parathyroid glands.
