Valorization of agro-food wastes: Ease of concomitant-enzymes production with application in food and biofuel industries.

The novel and greener approach toward the co-production of hydrolytic enzymes in a single-cultivation medium with inexpensive substrates can bring down the production costs. Likewise, the natural and industrial organic biomass/solid are all nutritionally rich substrates waiting for free use in industries such as food, biofuel, etc. Valorization must broaden its applications in industries and households with a step towards a sustainable environment. The biofuel approach can be projected as one of the most promising deputations to meet future energy demands, in reduction of the environmental pollution due to excessive fossil fuel consumption. The present review highlights the multifaceted stature of microbial enzymes in this direction and possible implications mainly in the food industry and biofuel with the global impact of similar bio-based industries. In this review, design scale-up, fermentation cost, energy needs,and agro-food waste management have been meticulously delineated.

Understanding the Basis of Occurrence, Biosynthesis, and Implications of Thermostable Alkaline Proteases.

The group of hydrolytic enzymes synonymously known as proteases is predominantly most favored for the class of industrial enzymes. The present work focuses on the thermostable nature of these proteolytic enzymes that occur naturally among mesophilic and thermophilic microbes. The broad thermo-active feature (40-80 °C), ease of cultivation, maintenance, and bulk production are the key features associated with these enzymes. Detailing of contemporary production technologies, and controllable operational parameters including the purification strategies, are the key features that justify their industrial dominance as biocatalysts. In addition, the rigorous research inputs by protein engineering and enzyme immobilization studies add up to the thermo-catalytic features and application capabilities of these enzymes. The work summarizes key features of microbial proteases that make them numero-uno for laundry, biomaterials, waste management, food and feed, tannery, and medical as well as pharmaceutical industries. The quest for novel and/or designed and engineered thermostable protease from unexplored sources is highly stimulating and will address the ever-increasing industrial demands.