ABSTRACT
A national citizen survey quantified the abundance of epiphytic lichens that are known to be either sensitive or tolerant to nitrogen (N) deposition. Records were collected across the UK from over 10,000 individual trees of 22 deciduous species. Mean abundance of tolerant and sensitive lichens was related to mean N deposition rates and climatic variables at a 5 km scale, and the response of lichens was compared on the three most common trees (Quercus, Fraxinus and Acer) and by assigning all 22 tree species to three bark pH groups. The abundance of N-sensitive lichens on trunks decreased with increasing total N deposition, while that of N-tolerant lichens increased. The abundance of N-sensitive lichens on trunks was reduced close to a busy road, while the abundance of N-tolerant lichens increased. The abundance of N-tolerant lichen species on trunks was lower on Quercus and other low bark pH species, but the abundance of N-sensitive lichens was similar on different tree species. Lichen abundance relationships with total N deposition did not differ between tree species or bark pH groups. The response of N-sensitive lichens to reduced nitrogen was greater than to oxidised N, and the response of N-tolerant lichens was greater to oxidised N than to reduced N. There were differences in the response of N-sensitive and N-tolerant lichens to rainfall, humidity and temperature. Relationships with N deposition and climatic variables were similar for lichen presence on twigs as for lichen abundance on trunks, but N-sensitive lichens increased, rather than decreased, on twigs of Quercus/low bark pH species. The results demonstrate the unique power of citizen science to detect and quantify the air pollution impacts over a wide geographical range, and specifically to contribute to understanding of lichen responses to different chemical forms of N deposition, local pollution sources and bark chemistry.
Subject(s)
Air Pollutants/analysis , Environmental Monitoring/methods , Lichens/chemistry , Nitrogen/analysis , Acer , Air Pollution/statistics & numerical data , Environmental Pollution , Lichens/physiology , Quercus , Trees , United KingdomABSTRACT
Consumer products are frequently and regularly used in the domestic environment. Realistic estimates for product use are required for exposure modelling and health risk assessment. This paper provides significant data that can be used as input for such modelling studies. A European survey was conducted, within the framework of the DG Sanco-funded EPHECT project, on the household use of 15 consumer products. These products are all-purpose cleaners, kitchen cleaners, floor cleaners, glass and window cleaners, bathroom cleaners, furniture and floor polish products, combustible air fresheners, spray air fresheners, electric air fresheners, passive air fresheners, coating products for leather and textiles, hair styling products, spray deodorants and perfumes. The analysis of the results from the household survey (1st phase) focused on identifying consumer behaviour patterns (selection criteria, frequency of use, quantities, period of use and ventilation conditions during product use). This can provide valuable input to modelling studies, as this information is not reported in the open literature. The above results were further analysed (2nd phase), to provide the basis for the development of 'most representative worst-case scenarios' regarding the use of the 15 products by home-based population groups (housekeepers and retired people), in four geographical regions in Europe. These scenarios will be used for the exposure and health risk assessment within the EPHECT project. To the best of our knowledge, it is the first time that daily worst-case scenarios are presented in the scientific published literature concerning the use of a wide range of 15 consumer products across Europe.
Subject(s)
Environmental Pollutants/analysis , Household Products/statistics & numerical data , Environmental Policy , EuropeABSTRACT
This paper highlights the development and application of the probabilistic model (IAPPEM), which predicts PM10 and PM2.5 concentrations in the indoor environments. A number of features are detailed and justified through simulated comparison, which are shown to be necessary when modelling indoor PM concentrations. A one minute resolution predicts up to 20% higher peak concentrations compared with a 15 min resolution. A modified PM10 deposition method, devised to independently analyse the PM2.5 fraction of PM10, predicts up to 56% higher mean concentrations. The application of the model is demonstrated by a number of simulations. The total PM contribution, from different indoor emission sources, was analysed in terms of both emission strength and duration. In addition, PM concentrations were examined by varying the location of the emission source. A 24-hour sample profile is simulated based on sample data, designed to demonstrate the combined functionality of the model, predicting PM10 and PM2.5 peak concentrations up to 1107±175 and 596±102 µg m(-3) respectively, whilst predicting PM10 and PM2.5 mean concentrations up to 259±21 and 166±11 µg m(-3) respectively.
Subject(s)
Air Pollutants/analysis , Air Pollution, Indoor/statistics & numerical data , Environmental Monitoring/methods , Models, Statistical , Particulate Matter/analysis , Air Pollution, Indoor/analysisABSTRACT
This study derived Species Sensitivity Distributions (SSD), representing a cumulative stressor-response distribution based on single-species sensitivity data, for ozone exposure on natural vegetation. SSDs were constructed for three species groups, i.e. trees, annual grassland and perennial grassland species, using species-specific exposure-response data. The SSDs were applied in two ways. First, critical levels were calculated for each species group and compared to current critical levels for ozone exposure. Second, spatially explicit estimates of the potentially affected fraction of plant species in Northwestern Europe were calculated, based on ambient ozone concentrations. We found that the SSD-based critical levels were lower than for the current critical levels for ozone exposure, with conventional critical levels for ozone relating to 8-20% affected plant species. Our study shows that the SSD concept can be successfully applied to both derive critical ozone levels and estimate the potentially affected species fraction of plant communities along specific ozone gradients.
Subject(s)
Air Pollutants/toxicity , Ozone/toxicity , Plants/drug effects , Stress, Physiological , Environmental Monitoring , Risk Assessment , Species SpecificityABSTRACT
We investigated the effects of different ratios of reduced (NH4+) versus oxidised (NO3(-)) nitrogen in deposition on heathland and species-rich grassland vegetation at high nitrogen deposition levels in large mesocosms filled with nutrient-poor soils to which different NH4+/NO3(-) ratios were applied. The response of the forbs, Antennaria dioica, Arnica montana, Gentiana pneumonanthe, Thymus serpyllum, the grasses Danthonia decumbens, Deschampsia flexuosa, Nardus stricta and the shrub Calluna vulgaris was recorded. The forb A. dioica and the grass D.decumbens preferred low NH4+/NO3(-) ratios and were characterised by a negative correlation between NH4+/NO3(-) ratios and biomass and survival, whereas the grasses N. stricta and D. flexuosa showed no correlation with NH4+/NO3(-) ratios. Lime addition eliminated the negative effects of high NH4+ concentrations in deposition for A. dioica and the grass D. decumbens. The implications of these findings for heathland vegetations are discussed.
Subject(s)
Ammonia/pharmacology , Ecology/methods , Environmental Pollutants/pharmacology , Nitrogen Oxides/pharmacology , Plant Development , Amino Acids/analysis , Biodiversity , Biomass , Calluna/chemistry , Calluna/growth & development , Chlorophyll/analysis , Hydrogen-Ion Concentration , Plants/chemistry , Plants, Edible/chemistry , Plants, Edible/growth & development , Poaceae/chemistry , Poaceae/growth & development , Soil/analysis , SpectrophotometryABSTRACT
Two very different types of approaches are currently in use today for indicating risk of ozone damage to vegetation in Europe. One approach is the so-called AOTX (accumulated exposure over threshold of Xppb) index, which is based upon ozone concentrations only. The second type of approach entails an estimate of the amount of ozone entering via the stomates of vegetation, the AFstY approach (accumulated stomatal flux over threshold of Y nmol m(-2) s(-1)). The EMEP chemical transport model is used to map these different indicators of ozone damage across Europe, for two illustrative vegetation types, wheat and beech forests. The results show that exceedences of critical levels for either type of indicator are widespread, but that the indicators give very different spatial patterns across Europe. Model simulations for year 2020 scenarios suggest reductions in risks of vegetation damage whichever indicator is used, but suggest that AOT40 is much more sensitive to emission control than AFstY values.
Subject(s)
Crops, Agricultural/drug effects , Fagus/drug effects , Oxidants, Photochemical/toxicity , Ozone/toxicity , Triticum/drug effects , Ecosystem , Environmental Exposure/adverse effects , Environmental Monitoring/methods , Europe , Models, Biological , Risk Assessment/methodsABSTRACT
A multiplicative and a semi-mechanistic, BWB-type [Ball, J.T., Woodrow, I.E., Berry, J.A., 1987. A model predicting stomatal conductance and its contribution to the control of photosynthesis under different environmental conditions. In: Biggens, J. (Ed.), Progress in Photosynthesis Research, vol. IV. Martinus Nijhoff, Dordrecht, pp. 221-224.] algorithm for calculating stomatal conductance (g(s)) at the leaf level have been parameterised for two crop and two tree species to test their use in regional scale ozone deposition modelling. The algorithms were tested against measured, site-specific data for durum wheat, grapevine, beech and birch of different European provenances. A direct comparison of both algorithms showed a similar performance in predicting hourly means and daily time-courses of g(s), whereas the multiplicative algorithm outperformed the BWB-type algorithm in modelling seasonal time-courses due to the inclusion of a phenology function. The re-parameterisation of the algorithms for local conditions in order to validate ozone deposition modelling on a European scale reveals the higher input requirements of the BWB-type algorithm as compared to the multiplicative algorithm because of the need of the former to model net photosynthesis (A(n)).
Subject(s)
Algorithms , Oxidants, Photochemical/toxicity , Ozone/toxicity , Plant Leaves/physiology , Betula/drug effects , Betula/physiology , Circadian Rhythm , Crops, Agricultural/drug effects , Crops, Agricultural/physiology , Environmental Exposure/adverse effects , Environmental Monitoring/methods , Fagus/drug effects , Fagus/physiology , Models, Biological , Plant Leaves/drug effects , Seasons , Triticum/drug effects , Triticum/physiology , Vitis/drug effects , Vitis/physiologyABSTRACT
Regional scale modelling of both ozone deposition and the risk of ozone impacts is poorly developed for grassland communities. This paper presents new predictions of stomatal ozone flux to grasslands at five different locations in Europe, using a mechanistic model of canopy development for productive grasslands to generate time series of leaf area index and soil water potential as inputs to the stomatal component of the DO(3)SE ozone deposition model. The parameterisation of both models was based on Lolium perenne, a dominant species of productive pasture in Europe. The modelled seasonal time course of stomatal ozone flux to both the whole canopy and to upper leaves showed large differences between climatic zones, which depended on the timing of the start of the growing season, the effect of soil water potential, and the frequency of hay cuts. Values of modelled accumulated flux indices and the AOT40 index showed a five-fold difference between locations, but the locations with the highest flux differed depending on the index used; the period contributing to the accumulation of AOT40 did not always coincide with the modelled period of active ozone canopy uptake. Use of a fixed seasonal profile of leaf area index in the flux model produced very different estimates of annual accumulated total canopy and leaf ozone flux when compared with the flux model linked to a simulation of canopy growth. Regional scale model estimates of both the risks of ozone impacts and of total ozone deposition will be inaccurate unless the effects of climate and management in modifying grass canopy growth are incorporated.
Subject(s)
Lolium/drug effects , Oxidants, Photochemical/toxicity , Ozone/toxicity , Biomass , Climate , Environmental Exposure/adverse effects , Europe , Lolium/growth & development , Lolium/metabolism , Oxidants, Photochemical/pharmacokinetics , Ozone/pharmacokinetics , Plant Leaves/drug effects , Plant Leaves/growth & development , Plant Leaves/metabolism , Plant Shoots/drug effects , Plant Shoots/growth & development , Plant Shoots/metabolism , Risk Assessment/methods , Seasons , Soil/analysis , Time Factors , Water/chemistryABSTRACT
The use of the herbarium moss archive for investigating past atmospheric deposition of Ni, Cu, Zn, As, Cd and Pb was evaluated. Moss samples from five UK regions collected over 150 years were analysed for 26 elements using ICP-MS. Principal components analysis identified soil as a significant source of Ni and As and atmospheric deposition as the main source of Pb and Cu. Sources of Zn and Cd concentrations were identified to be at least partly atmospheric, but require further investigation. Temporal and spatial trends in metal concentrations in herbarium mosses showed that the highest Pb and Cu levels are found in Northern England in the late 19th century. Metal concentrations in herbarium moss samples were consistently higher than those in mosses collected from the field in 2000. Herbarium moss samples are concluded to be a useful resource to contribute to reconstructing trends in Pb and Cu deposition, but not, without further analysis, for Cd, Zn, As and Ni.
Subject(s)
Bryophyta/chemistry , Environmental Pollution/analysis , Metals, Heavy/analysis , Time , Air Pollutants/analysis , Arsenic/analysis , Cadmium/analysis , Copper/analysis , England , Lead/analysis , Mass Spectrometry , Nickel/analysis , Preservation, Biological , Principal Component Analysis , Soil/analysis , Soil Pollutants/analysis , United Kingdom , Zinc/analysisABSTRACT
Although ozone has been shown to reduce the growth of individual species and to alter the composition of simple species mixtures, there is little understanding of its long-term effects on species dynamics and composition in real communities. Intact turfs of calcareous grassland were exposed to four different ozone regimes in open-top chambers over three consecutive summers. Treatments provided a mean seasonal AOT40 ranging from approximately zero to 15 ppm h. Cumulative ozone exposure was a significant factor in compositional change, but only explained 4.6% of the variation. The dominant grass species (Festuca rubra) showed a consistent decline in cover in the high ozone treatment over time and the forb Campanula rotundifolia was lost from all three ozone treatments. The frequency of some species (Galium verum and Plantago lanceolata) increased with ozone exposure. Long-term effects of ozone on species composition in chalk grassland may be a function of both the sensitivity of individual species and the response of the dominant species.
Subject(s)
Air Pollutants/toxicity , Ecosystem , Ozone/toxicity , Poaceae/drug effects , Biodiversity , Calcium , England , Geologic Sediments , Poaceae/growth & development , SeasonsABSTRACT
A study was undertaken to investigate the feasibility of using existing data sets of total soil metal concentrations and soil parameters, such as pH, to predict available metal concentrations on a regional or national basis. The attraction of such an approach is that it would provide valuable data for initiatives requiring information on the availability and mobility of metals in soils without the need for costly soil sampling and analysis. Ninety-seven topsoil and subsoil samples were collected from 6 soil series in a catenary sequence in north Wales to provide data for the development of an empirical model. These were analysed for total, 0.01 M CaCl2-extractable and porewater metal concentrations and for a range of soil properties including pH, solid and dissolved organic matter and cation exchange capacity. Regression analysis showed that, of the soil parameters measured, pH was the most important predictor variable for the estimation of CaCl2-extractable Cd, Pb and Zn. pH accounted for up to 86% of the variance in the proportion of 'total' metals which were extracted by CaCl2, a reagent that is commonly used to estimate plant uptake of elements. However, the relationships recorded between soil parameters and Kd (total metal/porewater metal) were much weaker, indicating that porewater metal concentrations can less readily be predicted from total soil metal concentrations and soil properties.
Subject(s)
Cadmium/chemistry , Calcium Chloride/chemistry , Lead/chemistry , Soil Pollutants , Zinc/chemistry , Cadmium/analysis , Environmental Monitoring , Hydrogen-Ion Concentration , Lead/analysis , Soil/analysis , Soil Pollutants/analysis , Solubility , Wales , Zinc/analysisABSTRACT
Ninety-eight surface soils were sampled from the uplands of England and Wales, and analysed for loss-on-ignition (LOI), and total and dissolved base cations, Al, Fe, and trace heavy metals (Cu, Zn, Cd, Pb). The samples covered wide ranges of pH (3.4-8.3) and LOI (9-98%). Soil metal contents measured by extraction with 0.43 mol l-1 HNO3 and 0.1 mol l-1 EDTA were very similar, and generally lower than values obtained by extraction with a mixture of concentrated nitric and perchloric acids. Total heavy metal concentrations in soil solution depend positively upon soil metal content and [DOC], and negatively upon pH and LOI, values of r2 ranging from 0.39 (Cu) to 0.81 (Pb). Stronger correlations (r2=0.76-0.95) were obtained by multiple regression analysis involving free metal ion (Cu2+, Zn2+, Cd2+, Pb2+) concentrations calculated with the equilibrium speciation model WHAM/Model VI. The free metal ion concentrations depend positively upon MHNO3 and negatively upon pH and LOI. The data were also analysed by using WHAM/Model VI to describe solid-solution interactions as well as solution speciation; this involved calibrating each soil sample by adjusting the content of "active" humic matter to match the observed soil pH. The calibrated model provided fair predictions of total heavy metal concentrations in soil solution, and predicted free metal ion concentrations were in reasonable agreement with the values obtained from solution-only speciation calculations.
Subject(s)
Metals, Heavy/analysis , Soil Pollutants/analysis , Cadmium/analysis , Copper/analysis , England , Environmental Monitoring/methods , Lead/analysis , Metals, Heavy/chemistry , Models, Chemical , Regression Analysis , Wales , Zinc/analysisABSTRACT
⢠Species of fen and fen-meadow communities, well supplied with water and nutrients, are characterised by high rates of growth, stomatal conductance values and specific leaf areas, all factors which have been associated with high sensitivity to ozone. We therefore examined the effects of ozone on 12 characteristic fen and fen-meadow species. ⢠Plants received either filtered air or ozone; AOT40 exposures ranged from 9200 to 14 300 ppb h. Eight of the 12 species exhibited foliar injury in response to ozone exposure, with the first signs of injury on Vicia cracca, following an AOT40 exposure of only 1950 pbb h. ⢠Ozone exposure significantly reduced plant photosynthetic rate, stomatal conductance and biomass production in four species. Cirsium arvense exhibited the greatest biomass response to ozone (32% and 58% reduction in above- and below-ground weight, respectively). Species with higher levels of visible injury tended to show greater reductions in biomass. There was a significant positive association between stomatal conductance and the magnitude of ozone effects on root biomass. ⢠The widespread occurrence of either visible injury or growth reductions amongst the species screened, and the magnitude of effects on the most sensitive species, indicate that species of fens and fen-meadows may be more sensitive to ozone than other seminatural ecosystems which have been the focus of recent ozone studies.
ABSTRACT
Plants of soybean (Glycine max L.) were grown with and without the ozone protectant EDU (N-[2-(2-oxo-1-imidazolidinyl)ethyl]-n2 phenylurea) at a suburban site, a remote rural site and a rural roadside site around the city of Lahore, Pakistan. The development and yield of the plants was determined in two experiments--one immediately post-monsoon and one in the following spring (pre-monsoon). Concentrations of nitrogen dioxide and photochemical oxidants were measured at each site. The effect on yield of EDU at the suburban site (47 and 113% increase in seed weight per plant relative to the untreated plants in the post- and pre-monsoon experiments, respectively) was similar to the effects of filtration on yield on soybean in a parallel open-top chamber study at the same site (77% increase relative to plants subjected to unfiltered air for the pre-monsoon experiment). Effects of EDU on yield were greater at both rural sites than at the suburban site in both experiments, and greater in the spring experiment (182% at the remote rural site and 285% at the rural roadside site) than in the post-monsoon experiment (94% at the remote rural site and 170% at the rural roadside site); oxidant concentrations were also greater at the rural sites than at the suburban site, and greater in the spring experiment than the post-monsoon experiment. The results imply that ozone may be causing significant crop losses in rural areas around Lahore; however, the geographical extent of the problem, and the implications for peri-urban agriculture around other cities of south Asia are uncertain.
Subject(s)
Air Pollutants/adverse effects , Fabaceae/growth & development , Oxidants, Photochemical/adverse effects , Ozone/adverse effects , Plants, Medicinal , Agriculture , Antioxidants/pharmacology , Phenylurea Compounds/pharmacology , Rural Population , SeasonsABSTRACT
Atmospheric nitrogen deposition has been shown to affect both the structure and the function of heathland ecosystems. Heathlands are semi-natural habitats and, as such, undergo regular management by mowing or burning. Different forms of management remove more or less nutrients from the system, so habitat management has the potential to mitigate some of the effects of atmospheric deposition. Data from a dynamic vegetation model and two field experiments are presented. The first involves nitrogen addition following different forms of habitat management. The second tests the use of habitat management to promote heathland recovery after a reduction in nitrogen deposition. Both modelling and experimental approaches suggest that plant and microbial response to nitrogen is affected by management. Shoot growth and rates of decomposition were lowest in plots managed using more intensive techniques, including mowing with litter removal and a high temperature burn. Field data also indicate that ecosystem recovery from prolonged elevated inputs of nitrogen may take many years, or even decades, even after the removal of plant and litter nitrogen stores which accompanies the more intensive forms of habitat management.
Subject(s)
Ecosystem , Nitrogen/metabolism , Atmosphere/chemistry , Biomass , Calluna/physiology , Climate , England , Models, Biological , Poaceae/physiology , Soil/analysis , Time FactorsABSTRACT
⢠A direct assessment of carbon distribution was made in fruiting and deblossomed strawberry (Fragaria × ananassa) in order to investigate the mechanisms of ozone (O3 ) action on fruit yield. ⢠Fruiting and deblossomed strawberry plants were treated with 92 ppb ozone or filtered air in open-top chambers for 69 d. Either leaf 1 or leaf 3 (from the most recent fully expanded) were exposed to a 30-min 14 CO2 pulse. The sink strength and relative specific uptake of sink tissues were determined after 24 h and 48 h. ⢠Ozone did not affect the sink strength of fruits, but there was a trend to decreasing relative specific uptake of fruits when leaf 1, but not when leaf 3, was labelled. Ozone increased the sink strength of leaf blades younger than the source leaf when leaf 3 was labelled. Leaf 3, but not leaf 1, distributed assimilates mostly to the fruit. Ozone increased the sink strength of petioles in deblossomed plants. ⢠There were important differences between source leaves of different age, both in overall carbon allocation to different sinks and the effects of ozone, which influenced the relative importance for fruit yield of effects of ozone on photosynthesis and on carbon allocation.
ABSTRACT
It has been proposed that stomatal flux of ozone would provide a more reliable basis than ozone exposure indices for the assessment of the risk of ozone damage to vegetation across Europe. However, implementation of this approach requires the development of appropriate models which need to be rigorously tested against actual data collected under field conditions. This paper describes such an assessment of the stomatal component of the model described by Emberson et al. (2000. Modelling stomatal ozone flux across Europe. Environmental Pollution 110). Model predictions are compared with field measurements of both stomatal conductance (g(s)) and calculated ozone flux for shoots of mature Norway spruce (Picea abies) growing in the Tyrol Mountains in Austria. The model has been developed to calculate g(s) as a function of leaf phenology and four environmental variables: photosynthetic flux density (PFD), temperature, vapour pressure deficit (VPD) and soil moisture deficit (SMD). The model was run using climate data measured on site, although the SMD component was omitted since the necessary data were not available. The model parameterisation for Norway spruce had previously been collected from the scientific literature and therefore established independently from the measurement study. Overall, strong associations were found between model predictions and measured values of stomatal conductance to ozone (GO(3)) and calculated stomatal ozone flux (FO(3)). Average diurnal profiles of GO(3) and FO(3) showed good agreement between the field data and modelled values except during the morning period of 1990. The diurnal pattern of ozone flux was determined primarily by PFD and VPD, as there was little diurnal variation in ozone concentration. In general, the model predicted instances of high ozone flux satisfactorily, indicating its potential applicability in identifying areas of high ozone risk for this species.
ABSTRACT
A model has been developed to estimate stomatal ozone flux across Europe for a number of important species. An initial application of this model is illustrated for two species, wheat and beech. The model calculates ozone flux using European Monitoring and Evaluation Programme (EMEP) model ozone concentrations in combination with estimates of the atmospheric, boundary layer and stomatal resistances to ozone transfer. The model simulates the effect of phenology, irradiance, temperature, vapour pressure deficit and soil moisture deficit on stomatal conductance. These species-specific microclimatic parameters are derived from meteorological data provided by the Norwegian Meteorological Institute (DNMI), together with detailed land-use and soil type maps assembled at the Stockholm Environment Institute (SEI). Modelled fluxes are presented as mean monthly flux maps and compared with maps describing equivalent values of AOT40 (accumulated exposure over threshold of 40 ppb or nl l(-1)), highlighting the spatial differences between these two indices. In many cases high ozone fluxes were modelled in association with only moderate AOT40 values. The factors most important in limiting ozone uptake under the model assumptions were vapour pressure deficit (VPD), soil moisture deficit (for Mediterranean regions in particular) and phenology. The limiting effect of VPD on ozone uptake was especially apparent, since high VPDs resulting in stomatal closure tended to co-occur with high ozone concentrations. Although further work is needed to link the ozone uptake and deposition model components, and to validate the model with field measurements, the present results give a clear indication of the possible implications of adopting a flux-based approach for future policy evaluation.
ABSTRACT
Fruiting and deblossomed plants of strawberry (Fragaria×ananassa) were exposed to 92 ppb ozone or filtered air in open-top chambers for 69 d. Flower and fruit production, relative growth rate of leaf area, leaf gas exchange and plant biomass were investigated. Ozone caused an initial acceleration in inflorescence production, which was followed by a reduction in inflorescence production, fruit set, and, later, individual fruit weight, although total fruit yield was not affected before the end of the fumigation period. Ozone accelerated leaf senescence and had a greater negative effect on the rate of photosynthesis in older than in younger leaves in fruiting and deblossomed plants, but the response of net photosynthesis to ozone did not differ between the two groups of plants. Relative growth rate of leaf area was the first parameter to be reduced by ozone fumigation, with the effect being significant in fruiting, but not in deblossomed, plants. Final above-ground biomass was also significantly decreased by ozone in fruiting plants, but not in deblossomed plants. Root and crown biomass were not significantly affected by ozone fumigation in either fruiting or deblossomed plants.
ABSTRACT
The evidence of detrimental effects of ozone on vegetation in Europe, and the need to develop international control policies to reduce ozone exposures which are based on the effects of the pollutant, has led to attempts to define so-called critical levels of ozone above which adverse effects on trees, crops and natural vegetation may occur. This review is a critical assessment of the scientific basis of the concepts used to define critical levels for ozone and identifies the key limitations and uncertainties involved. The review focuses on the Level I critical level approach, which provides an environmental standard or threshold to minimise the effects of ozone on sensitive receptors, but does not seek to quantify the impacts of exceeding the critical level under field conditions. The concept of using the AOT (accumulated exposure over a threshold) to define long-term ozone exposure is demonstrated to be appropriate for several economically important species. The use of 40 ppb (giving the AOT40 index) as a threshold concentration gives a good linear fit to experimental data from open-top chambers for arable crops, but it is less certain that it provides the best fit to data for trees or semi-natural communities. Major uncertainties in defining critical level values relate to the choice of response parameter and species; the absence of data for many receptors, especially those of Mediterranean areas; and extrapolation to field conditions from relatively short-term open-top chamber experiments. The derivation of critical levels for long-lived organisms, such as forest trees, may require the use of modelling techniques based on physiological data from experimental studies. The exposure-response data which have been applied to derive critical levels should not be used to estimate the impacts of ozone over large areas, because of the uncertainties associated with extrapolation from the open-top chamber method, especially for forest trees, and because of spatial variation in atmospheric and environmental conditions, which may alter ozone uptake.