Retrospective analysis of vitamin D status on inflammatory markers and course of the disease in patients with COVID-19 infection.

PURPOSE: The aim of the study was to investigate the association between serum 25-hydroxyvitamin D status within the last 6 months prior to COVID-19 infection and parameters of immune function and clinical outcomes. METHODS: Fifty-six patients, who were admitted to the emergency clinic and diagnosed with COVID-19 infection, were included in the study. Data on clinical characteristics, inflammatory parameters and vitamin D status were recorded for each patient. All the participants had data on 25-hydroxyvitamin D status within the last 6 months prior to COVID-19 infection. RESULTS: The patients were stratified as those with vitamin D status less than 20 ng/mL and higher than 20 ng/mL. A group with vitamin D status less than 20 ng/mL had lower lymphocyte counts and lower haemoglobin levels that was statistically significant (respectively; p = 0.021, p = 0.035). Higher C-reactive protein (CRP) levels were seen in the vitamin D-deficient group (p = 0.013). It was observed that vitamin D status of the patients who required oxygen therapy were lower than those who did not require oxygen therapy, not statistically significant (p = 0.05). Patients who did not use vitamin D supplementation within 6 months prior to COVID-19 infection had more likely to be diagnosed with pneumonia (p = 0.004). CONCLUSION: Cases with lower vitamin D status had increased inflammatory markers and worse clinical outcomes than patients with higher vitamin D status. This study suggests that vitamin D status can be used as a prognostic factor in COVID-19 patients, and vitamin D supplementation can be recommended to improve the clinical outcomes in COVID-19 infection.

Variations in serum metabolites in response to season, cyclicity, and pregnancy in estrus-synchronized Damascus goats.

The current study was carried out to determine the changes in serum electrolytes, liver enzymes, and hormones during breeding and non-breeding seasons in estrus-synchronized goats. Forty goats (n = 40) were synchronized by inserting the sponges intravaginally for 12 days; PMSG and PGF2α were injected on day of sponge removal. Blood samples were collected from the goats at the time of sponges insertion (day -12), estrus day (day 0), and at 15 day after sponge withdrawal (day 15). The blood samples were analyzed for glucose, cholesterol (CHO), aspartate aminotransferase (AST), alanine aminotransferase (ALT), thyroid stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4) and progesterone (P4), calcium (Ca), phosphate (PO4), magnesium (Mg), sodium (Na), potassium (K), and chlorine (Cl) levels. Repeated measurements ANOVA of general linear model were used as statistical model. The results showed that seasons have an effect (p < 0.05) on glucose, Ca, Na, K, Cl, CHO, T3, and T4 levels. There was an effect (p < 0.05) of cyclicity only on P4 level. Whereas, nonpregnant, or goats carrying singlet and twin fetuses have shown variation (p < 0.05) in glucose, Ca, and P4 levels. Although, the correlations existed between different serum metabolites but strong correlations (p < 0.05) were observed either between Ca and Na or T3 and T4. In conclusion, the serum electrolytes and liver enzymes are correlated with hormones during the different seasons, pregnancy status, and fecundity. In recommendation, the results of the study could be as managemental tool to monitor the reproductive activity across seasons and to maintain pregnancy carrying twining in goat breeds.

Dual suppression with oral contraceptive pills in GnRH antagonist cycles for patients with polycystic ovary syndrome undergoing intracytoplasmic sperm injection.

OBJECTIVE: To evaluate the effects of a gonadotropin-releasing hormone (GnRH) antagonist protocol, with or without oral contraceptive pill (OCP) pretreatment, in patients with polycystic ovary syndrome (PCOS) undergoing intracytoplasmic sperm injection (ICSI). STUDY DESIGN: In this retrospective cohort study, 410 infertile patients with PCOS were assessed in their first ICSI cycles between January 2006 and June 2013. In Group A (n=208), patients underwent a long luteal GnRH agonist protocol, and in Groups B (n=143) and C (n=59), patients underwent a GnRH antagonist protocol. The patients in Group C also received OCPs containing 30mg of ethinyl oestradiol and 3mg of drospirenone prior to treatment. The main outcome measures were pregnancy and ovarian hyperstimulation syndrome (OHSS) rates. RESULTS: Demographic features, body mass index, duration of infertility, serum baseline hormone levels, cycle outcomes, multiple pregnancy rates, miscarriage rates, OHSS rates, total number of Grade A embryos and total number of transferred embryos were comparable between the groups. Clinical pregnancy rates were 27.4%, 26.6% and 23.7% in Groups A, B and C, respectively (p=0.853). CONCLUSIONS: OCP pretreatment was found to have no beneficial or adverse effects in patients with PCOS undergoing a GnRH antagonist protocol for ICSI, but can be used for cycle scheduling.

Impact of unilateral tubal blockage diagnosed by hysterosalpingography on the success rate of treatment with controlled ovarian stimulation and intrauterine insemination.

The aim of this study was to evaluate the success rates of intrauterine insemination (IUI) in infertile women with unilateral proximal and distal tubal blockage. A total of 161 couples with unilateral tubal blockage and unexplained infertility were included. The primary outcome measure was the cumulative pregnancy rate (CPR). The CPRs after three cycles of IUI were 26.3% (10/38) in patients with unilateral tubal blockage, and 44.7% (55/123) in patients with unexplained infertility (p = 0.043). CPRs were similar in patients with proximal unilateral tubal blockage and unexplained infertility (38.1% vs 44.7%, respectively, p = 0.572). CPR was significantly lower in patients with distal unilateral tubal blockage than in patients with unexplained infertility (11.7% vs 44.7%, respectively, p = 0.01). In conclusion, IVF instead of IUI may be a more appropriate approach for distal unilateral tubal blockage patients.

Fast and quasideterministic single ion source from a dipole-blockaded atomic ensemble.

We present a fast and quasideterministic protocol for the production of single ions and electrons from a cloud of laser-cooled atoms. The approach is based on a two-step process where first a single Rydberg atom is photoexcited from a dipole-blockade configuration and subsequently ionized by an electric field pulse. We theoretically describe these excitation-ionization cycles via dynamical quantum maps and observe a rich behavior of the ionization dynamics as a function of laser Rabi frequency, pulse duration, and particle number. Our results show that a fast sequential heralded production of single charged particles is achievable even from an unstructured and fluctuating atomic ensemble.

Nonadiabatic motional effects and dissipative blockade for Rydberg atoms excited from optical lattices or microtraps.

The laser excitation of Rydberg atoms in ultracold gases is often described assuming that the atomic motion is frozen during the excitation time. We show that this frozen gas approximation can break down for atoms that are held in optical lattices or microtraps. In particular, we show that the excitation dynamics is in general strongly affected by mechanical forces among the Rydberg atoms as well as the spread of the atomic wave packet in the confining potential. This causes decoherence in the excitation dynamics-resulting in a dissipative blockade effect-that renders the Rydberg excitation inefficient even in the antiblockade regime. For a strongly off-resonant laser excitation-usually considered in the context of Rydberg dressing-these motional effects compromise the applicability of the Born-Oppenheimer approximation. In particular, our results indicate that they can also lead to decoherence in the dressing regime.

Thermalization of a strongly interacting closed spin system: from coherent many-body dynamics to a Fokker-Planck equation.

Thermalization has been shown to occur in a number of closed quantum many-body systems, but the description of the actual thermalization dynamics is prohibitively complex. Here, we present a model-in one and two dimensions-for which we can analytically show that the evolution into thermal equilibrium is governed by a Fokker-Planck equation derived from the underlying quantum dynamics. Our approach does not rely on a formal distinction of weakly coupled bath and system degrees of freedom. The results show that transitions within narrow energy shells lead to a dynamics which is dominated by entropy and establishes detailed balance conditions that determine both the eventual equilibrium state and the nonequilibrium relaxation to it.

Dissipative binding of lattice bosons through distance-selective pair loss.

We show that in a gas of ultracold atoms distance selective two-body loss can be engineered via the resonant laser excitation of atom pairs to interacting electronic states. In an optical lattice this leads to a dissipative master equation dynamics with Lindblad jump operators that annihilate atom pairs with a specific interparticle distance. In conjunction with coherent hopping between lattice sites this unusual dissipation mechanism leads to the formation of coherent long-lived complexes that can even exhibit an internal level structure which is strongly coupled to their external motion. We analyze this counterintuitive phenomenon in detail in a system of hard-core bosons. While current research has established that dissipation in general can lead to the emergence of coherent features in many-body systems our work shows that strong nonlocal dissipation can effectuate a binding mechanism for particles.

Nonlocal nonlinear optics in cold Rydberg gases.

We present an analytical theory for the nonlinear optical response of a strongly interacting Rydberg gas under conditions of electromagnetically induced transparency. Simple formulas for the third-order optical susceptibility are derived and shown to be in excellent agreement with recent experiments. The obtained expressions reveal strong nonlinearities, which in addition are of highly nonlocal character. This property together with the enormous strength of the Rydberg-induced nonlinearities is shown to yield a unique laboratory platform for nonlinear wave phenomena, such as collapse-arrested modulational instabilities in a self-defocusing medium.

Two-dimensional Rydberg gases and the quantum hard-squares model.

We study a two-dimensional lattice gas of atoms that are photoexcited to Rydberg states in which they interact via the van der Waals interaction. We explore the regime of dominant nearest-neighbor interaction where this system is intimately connected with a quantum version of Baxter's hard-squares model. We show that the strongly correlated ground state of the Rydberg gas can be analytically described by a projected entangled pair state that constitutes the ground state of the quantum hard-squares model. This correspondence allows us to identify a phase boundary where the Rydberg gas undergoes a transition from a disordered (liquid) phase to an ordered (solid) phase.

Newton's cradle and entanglement transport in a flexible Rydberg chain.

In a regular, flexible chain of Rydberg atoms, a single electronic excitation localizes on two atoms that are in closer mutual proximity than all others. We show how the interplay between excitonic and atomic motion causes electronic excitation and diatomic proximity to propagate through the Rydberg chain as a combined pulse. In this manner entanglement is transferred adiabatically along the chain, reminiscent of momentum transfer in Newton's cradle.

Antiblockade in Rydberg excitation of an ultracold lattice gas.

It is shown that the two-step excitation scheme typically used to create an ultracold Rydberg gas can be described with an effective two-level rate equation, greatly reducing the complexity of the optical Bloch equations. This allows us to efficiently solve the many-body problem of interacting cold atoms with a Monte Carlo technique. Our results reproduce the observed excitation blockade effect. However, we demonstrate that an Autler-Townes double peak structure in the two-step excitation scheme, which occurs for moderate pulse lengths as used in the experiment, can give rise to an antiblockade effect. It is most pronounced for atoms arranged on a lattice. Since the effect is robust against a large number of lattice defects it should be experimentally realizable with an optical lattice created by CO2 lasers.

CYP2C19 polymorphisms in patients with gastric and colorectal carcinoma.

BACKGROUND: It has been reported that up to 80% of human cancers arise as a consequence of environmental exposure and host susceptibility factors. Environmental carcinogens are predominantly metabolized by the cytochrome P450 (CYP) superfamily of drug- or xenobiotic-metabolizing enzymes. Genetic variations in these enzymes affect individuals' susceptibility to carcinogens. AIM OF THE STUDY: The aim of this study was to evaluate the relationship between CYP2C19 polymorphism and susceptibility to these cancers by means of CYP2C19 genotyping among Turkish subjects. METHODS: DNAof subjects were isolated from leukocytes by high pure template preparation kit (Roche Diagnostics, GmbH, Mannheim, Germany) and genotypes were detected by LightCycler CYP2C19 Mutation Detection Kit by real-time PCR with LightCycler instrument (Roche Diagnostics, cat. no. 3113914). RESULTS: Being male was associated with a 3.5-fold (OR: 4.27, CI: 2.27-8.05) and 4.27-fold (OR: 3.50, CI: 1.948-6.301) risk for colorectal and gastric carcinoma, respectively. The CYP2C19*3 heterozygote genotype was not found in either gastric or colorectal carcinoma patients. Although the frequency of CYP2C19*2 heterozygote genotype is high in patients with gastric and colorectal carcinoma, it is not significantly associated with cancer (OR: 1.79, CI: 0.829-3.865 and OR: 1.998, CI: 0.961-4.154, respectively). CONCLUSION: Although the frequency of CYP2C19*2 heterozygote genotype is high in our patients with gastric and colorectal carcinoma, there is no the relationship between CYP2C19 polymorphism and susceptibility to these cancer.

Optimization of antiscorpion venom production

The present study was carried out to produce highly efficient antivenom from a small number of telsons in a short time. Venom solution was prepared through maceration of telsons from Androctonus crassicauda (Olivier, 1807) collected in the Southeastern Anatolia Region, Turkey. Lethal dose 50% (LD50) of the venom solution injected into mice was 1 ml/kg (95% confidence interval; 0.8-1.3), according to probit analysis. Different adjuvants (Freund's Complete Adjuvant, Freund's Incomplete Adjuvant, and 0.4% aluminium phosphate), at increasing doses and combined with venom, were subcutaneously injected into horses on days 0, 14, 21, 28, 35, and 42 of the experiment. Antivenom was collected from the immunized horses on days 45, 48, and 51 using the pepsin digestive method. The antivenom effective dose 50% (ED50) in mice was 0.5 ml (95% confidence interval; 0.40-0.58), according to probit analysis. It was concluded that 0.5 ml antivenom neutralized a venom dose 35-fold higher than the venom LD50. Thus, highly potent antivenom could be produced from about 238 telsons in 51 days.