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Future Microbiol ; 11: 865-75, 2016 07.
Article in English | MEDLINE | ID: mdl-27357520

ABSTRACT

AIM: To characterize the genetic diversity of unrelated Legionella pneumophila clinical isolates in Catalonia and compare with other European regions. METHODS: 95 unrelated isolates were analyzed using monoclonal antibodies and sequence-based typing, 1989-2013. RESULTS: The isolates showed a high diversity (IOD 0.964) with a predominance of some profiles (ST37-Phialdelphia, ST23-Philadelphia and ST1-OLDA). All regions had predominant sequence types (STs) that differed between regions, and only 3% of STs were shared between the three regions. CONCLUSION: L. pneumophila clinical isolates from Catalonia presented a high diversity and can be used in epidemiological surveillance studies. The heterogeneous predominance of STs between European regions suggested a relationship between geographical distribution and virulence of some STs.


Subject(s)
Bacterial Typing Techniques , Genetic Variation , Legionella pneumophila/classification , Legionella pneumophila/isolation & purification , Legionnaires' Disease/microbiology , Antibodies, Monoclonal , DNA, Bacterial/isolation & purification , Europe/epidemiology , Genotype , Humans , Legionella pneumophila/immunology , Legionnaires' Disease/epidemiology , Molecular Typing/methods , Sequence Analysis, DNA/methods , Serogroup , Serotyping
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