ABSTRACT
Light provides energy for photosynthesis and also acts as an important environmental signal. During their evolution, plants acquired sophisticated sensory systems for light perception and light-dependent regulation of their growth and development in accordance with the local light environment. Under natural conditions, plants adapted by using their light sensors to finely distinguish direct sunlight and dark in the soil, deep grey shade under the upper soil layer or litter, green shade under the canopy and even lateral green reflectance from neighbours. Light perception also allows plants to evaluate in detail the weather, time of day, day length and thus the season. However, in artificial lighting conditions, plants are confronted with fundamentally different lighting conditions. The advent of new light sources - light-emitting diodes (LEDs), which emit narrow-band light - allows growing plants with light of different spectral bands or their combinations. This sets the task of finding out how light of different quality affects the development and functioning of plants, and in particular, their photosynthetic apparatus (PSA), which is one of the basic processes determining plant yield. In this review, we briefly describe how plants perceive environment light signals by their five families of photoreceptors and by the PSA as a particular light sensor, and how they use this information to form their PSA under artificial narrow-band LED-based lighting of different spectral composition. We consider light regulation of the biosynthesis of photosynthetic pigments, photosynthetic complexes and chloroplast ATP synthase function, PSA photoprotection mechanisms, carbon assimilation reactions and stomatal development and function.
ABSTRACT
In plants, the short-term regulation (STR, seconds to minute time scale) of photosynthetic apparatus is associated with the energy-dependent control in the chloroplast electron transport, the distribution of light energy between photosystems (PS) II and I, activation/deactivation of the Calvin-Benson cycle (CBC) enzymes, and relocation of chloroplasts within the plant cell. In this work, using a dual-PAM technique for measuring the time-courses of P700 photooxidation and Chl a fluorescence, we have investigated the STR events in Tradescantia fluminensis leaves. The comparison of Chl a fluorescence and [Formula: see text] induction allowed us to investigate the contribution of the trans-thylakoid pH difference (ΔpH) to the STR events. Two parameters were used as the indicators of ΔpH generation: pH-dependent component of non-photochemical quenching of Chl a fluorescence, and pHin-dependent rate of electron transfer from plastoquinol (PQH2) to [Formula: see text] (via the Cyt b6f complex and plastocyanin). In dark-adapted leaves, kinetics of [Formula: see text] induction revealed three phases. Initial phase is characterized by rapid electron flow to [Formula: see text] (τ1/2 ~ 5-10 ms), which is likely related to cyclic electron flow around PSI, while the outflow of electrons from PSI is restricted by slow consumption of NADPH in the CBC. The light-induced generation of ΔpH and activation of the CBC promote photooxidation of P700 and concomitant retardation of [Formula: see text] reduction (τ1/2 ~ 20 ms). Prolonged illumination induces additional slowing down of electron transfer to [Formula: see text] (τ1/2 ≥ 30-35 ms). The latter effect is not accompanied by changes in the Chl a fluorescence parameters which are sensitive to ΔpH generation. We suggest the tentative explanation of the latter results by the reversal of Q-cycle, which causes the deceleration of PQH2 oxidation due to the back pressure of stromal reductants.