Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Database
Language
Publication year range
1.
J Comp Pathol ; 131(2-3): 199-206, 2004.
Article in English | MEDLINE | ID: mdl-15276859

ABSTRACT

Formalin-fixed, paraffin wax-embedded tissues of three Malaysian farm pigs naturally infected with Nipah virus were used to investigate the value of anti-Nipah virus mouse monoclonal antibodies (Mabs) and rabbit polyclonal antibody for immunohistochemical diagnosis. Mabs 11F6 and 12A5 gave intense immunolabelling in lung tissue that had been fixed in 10% neutral buffered formalin for about 4 years, whereas the reactivity of Mabs 13A5 and 18C4 and polyclonal antibody was reduced significantly by long-term formalin fixation. Immunohistochemical examination of Malaysian farm pig samples with Mab 11F6 confirmed the affinity of Nipah virus for respiratory epithelium, renal glomerular and tubular epithelium, meningeal arachnoidal cells, and systemic vascular endothelium and smooth muscle. In addition, Nipah virus antigens were identified in laryngeal epithelial cells, Schwann cells of peripheral nerve fascicles in the spleen, and endothelial cells in the atrioventricular valve. The study demonstrated the value of Mabs 11F6 and 12A5 for the immunohistochemical diagnosis of Nipah virus infection in pigs.


Subject(s)
Antibodies, Monoclonal , Henipavirus Infections/diagnosis , Henipavirus Infections/veterinary , Swine/virology , Animals , Antigens, Viral/immunology , Antigens, Viral/metabolism , Formaldehyde , Henipavirus Infections/immunology , Immunohistochemistry , Malaysia , Nipah Virus/immunology , Tissue Fixation
2.
Med J Malaysia ; 58(5): 653-6, 2003 Dec.
Article in English | MEDLINE | ID: mdl-15190649

ABSTRACT

How people perceive risk influences their behaviour towards these risks. We do not know how workers perceive risk of dying from diseases or accidents. This study was conducted among 198 workers of a security company in Malaysia. The workers were asked to score on a Likert scale of 1 to 5 the perceived risk of death of Malaysians from selected causes of death. The highest perceived risks of death were, in order of ranking, motor vehicle accidents, cancer and diabetes mellitus whereas according to the certified causes of death in Malaysia the highest risks of death among the selected items were cardiovascular disease, cancer and stroke. The difference in perception and mortality data needs be addressed.


Subject(s)
Attitude , Cause of Death , Adolescent , Adult , Aged , Female , Humans , Malaysia , Male , Middle Aged , Risk Factors
3.
Arch Virol ; 147(8): 1623-30, 2002 Aug.
Article in English | MEDLINE | ID: mdl-12181680

ABSTRACT

A virus, named Oya virus, was isolated in Vero cell cultures from the lungs of a pig suspected of Nipah virus infection. The virus was revealed as a spherical enveloped RNA virus with a diameter of 79 nm. For identification of Oya virus, RT-PCR was performed. A common primer set for S-RNA of the Simbu serogroup of the genus Bunyavirus was able to amplify a cDNA from Oya virus RNA. The sequence data of the product revealed that the partial gene of Oya virus S-RNA segment had 65-70% homology with published cDNA sequences of Simbu serogroup viruses. The phylogenetic analysis of the data showed that the Oya virus is grouped in Simbu serogroup, but is genetically distinct from the serogroup viruses that have been analyzed molecularly. Serological surveys revealed that the virus distributed widely and densely in Malaysia.


Subject(s)
Paramyxoviridae Infections/veterinary , Paramyxovirinae , Simbu virus/isolation & purification , Swine/virology , Animals , Antibodies, Viral/blood , Base Sequence , Chlorocebus aethiops , Cytopathogenic Effect, Viral , Molecular Sequence Data , Paramyxoviridae Infections/virology , Reverse Transcriptase Polymerase Chain Reaction , Simbu virus/classification , Vero Cells
4.
Metabolism ; 25(7): 803-7, 1976 Jul.
Article in English | MEDLINE | ID: mdl-781469

ABSTRACT

To determine whether centrally mediated adrenergic tone modulates lipolysis, ketogenesis, or insulinopenia during starvation, four lean male subjects with complete cervical cord transection and six lean healthy male volunteers were fasted for 48 hr. Plasma glucose and insulin levels decreased to comparable levels in both groups. Plasma free fatty acid (FFA) and beta-hydroxybutyrate concentrations rose to peak levels 1.23 +/- 0.08 mmoles/liter and 4.2 +/- 1.0 mmoles/liter at 36 and 48 hr in normals, respectively. Cord-sectioned subjects had similar peak FFA (1.2 "/- 0.12) and beta-hydroxybutyrate (5.6 +/- 0.3) concentrations. Urinary catecholamine excretion in four normal subjects failed to increase during the fast. Since normal glucose, free fatty acid, beta-hydroxybutyrate, and insulin relationships were maintained in sympathectomized subjects, it appears that central adrenergic mechanisms are not essential for initiation of lipolysis, ketonemia, or the hypoinsulinemia of early starvation. These results provide additional evidence that these metabolic events are primarily related to insulinopenia.


Subject(s)
Fasting , Metabolism , Sympathetic Nervous System/physiology , Adult , Blood Glucose/analysis , Catecholamines/urine , Clinical Trials as Topic , Fatty Acids, Nonesterified/blood , Humans , Hydroxybutyrates/blood , Insulin/blood , Lipid Metabolism , Lipid Mobilization , Male , Quadriplegia/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL