Cellulolytic bacteria isolation, screening and optimization of enzyme production from vermicompost of paper cup waste.

In the current scenario, used paper cups are disposed of without any proper treatment, thereby damaging the environment. Hence, the vermicomposting technique is preferred for managing these wastes. The ability of bacterial strains on cellulase enzyme (Endoglucanase, exoglucanase and ß-glucosidase) production at altered pH and temperatures were focused in this study. Among nine bacterial strains Acinetobacter baumannii was found to have high enzyme activity. HPLC analysis confirms that about 45% of cellulose degradation occurred due to the action of bacterial consortia at 37 °C with pH 7. The overall period required for degradation takes only three months with the help of bacterial consortia while comparing to our previous study, which takes six months. The insilico study on three cellulose-degrading enzymes sequence were retrieved from NCBI, and analysed for multiple sequence alignment and phylogenetic tree construction. From the analysis, the endoglucanase SVK46152 (Acinetobacter baumannii) sequence got docked with cellopentaose with a high score value -11.07. Thereby we confirm that organism Acinetobacter baumannii was effective in paper cup degradation.

Sustainable management strategies for bacterial wilt of sweet peppers (Capsicum annuum) and other Solanaceous crops.

Pepper bacterial wilt is caused by the bacterial pathogen, Ralstonia solanacearum. It is the most destructive disease of many Solanaceous crops such as potatoes, tobacco, pepper, tomatoes and eggplant and is a significant source of crop loss worldwide. Physical, cultural and chemical controls have been employed to combat this destructive disease. However, none of these strategies has been able to control the disease completely due to the broad host range and genetic diversity of the pathogen, its prolonged survival in the soil and survival on vegetation as a latent infection. Owing to co-management strategies, biological control is the best approach for human health and environmental friendly motivations. It makes use of various antagonistic rhizobacteria and epiphytic species such as Bacillus cereus, Pseudomonas putida, Bacillus subtilis, Paenibacillus macerans, Serratia marcescens, Bacillus pumilus and Pseudomonas fluorescens, which compete with and ultimately inhibit the growth of the pathogen. The possible mechanisms of biocontrol by these species involve multifaceted interactions between the host, pathogen and the antagonists. These can involve competition for nutrients and space, plant-mediated systemic resistance, siderophore production and production of extracellular cell wall degrading enzymes to inhibit or suppress the growth of the bacterial wilt agent.

Perceived Stress and its Relationship with Coping Strategies among Doctors at a Tertiary Hospital in Ile-Ife, Nigeria.

BACKGROUND: Medical doctors are prone to stress and utilize a variety of coping strategies. This study aims to measure the level of perceived stress and its relationship with coping strategies among doctors working in a tertiary hospital in Ile-Ife, Nigeria. METHODS: A cross-sectional, descriptive study using a self-administered questionnaire containing Perceived Stress Scale (PSS) and Brief COPE Scale to assess perceived stress and coping strategies among different cadres of doctors. Out of the 217 questionnaires distributed, 185 completed copies were analysed. RESULTS: Most of the respondents were less than 35 years (mean = 33.6 ±6.21) and the rate of perceived stress was 43.2%. The mean scores of Perceived Stress Scale and Brief COPE were 23.08 (±6.58) and 59.46 (±12.05). Socio-demographic factors significantly associated with perceived stress were age less than 35 years, being single, working in the hospital and years of practice less than 5 years and being house-officer or registrar (p< .001). Respondent that were not stressed reported significant higher mean scores in active coping and positive reframing but lower mean scores in humor (p< .05). Also, they reported significant lower mean scores in maladaptive coping and its subscales (p<.003). Perceived stress had a significant negative correlation with active coping (p=.017) and positive correlation with maladaptive coping and all its subscales (p< .006). Only age of respondents significantly predicted perceived stress among respondents (p = .003). CONCLUSION: There is high level of stress among a sample of Nigerian doctors. Hence, there is a need for stress management program among them.

Sub-chronic toxicity study of ethanol stem-bark extract of Blighia sapida (Sapindaceae) in wistar rats.

Blighia sapida has been used in the treatment of different pathologies. The study aimed at evaluating the acute and sub-chronic toxicity of ethanol stem-bark extract of B. sapida. The acute toxicity was evaluated by gavage administration at single dose and the extract was also administered at doses of 250, 500 and 750 mg/kg body weight every other day for ninety day. No mortality or observable signs of toxicity were observed for acute and sub-chronic effects of the extract on the tested animals. No significant difference (P > 0.05) in haematological and biochemical parameters compared to the control group. However, histopathological observation revealed some derangements which could be due to continuous consumption of the extract by the animals. It implied that care must be exercised in the use of the plant for a long period of time to prevent its possible long-term toxic effects.

Selenium levels in neoplastic breast lesions.

AIMS AND OBJECTIVES: The aim of this prospective case controlled study was to evaluate the tissue levels of selenium in patients with cases of fibroadenoma, cancer of the breast and in the controls in order to relate them to the occurrence of breast diseases. SUBJECTS AND METHODS: Consecutive consenting patients who had histologically confirmed breast cancer and fibroadenoma attending the General surgical outpatients departments of Obafemi Awolowo University Teaching Hospital Complex, Ile-Ife, were recruited for the study. One gram of core disease breast tissues was taken for Selenium level estimation. RESULTS: There were 127 female subjects;. 95 (74.8%) cases of fibroadenoma and 32 (25.2%) of breast cancer. While breast cancer was common on the left, fibroadenoma was more common on the right breast (? = 8.994; p=0.011). The median tissue level of selenium in patients with fibroadenoma was 0.0272 mg/g with a range of 0.0124 to 0.0576 mg/g and that of the cancer patients was 0.0178 mg/g with a range 0.0072 to 0.0436 mg/g. These were statistically significantly different ( p=0.001). Factors affecting tissue selenium level include age (p<0.001), overall stage of breast cancer (p<0.001), maximum length of breast mass (p=0.023), previous delivery (p=0.004), age at last confinement (p=0.007), parity (p<0.001), oestrogen receptor (ER) status (p<0.001) and progesterone receptor (PR) status (p=0.021). CONCLUSION: Tissue selenium was lower in breast cancer than in fibroadenoma; Tissue selenium inhibits carcinogenesis; low tissue level of selenium therefore may be a factor in the development of breast cancer.

High cadmium / zinc ratio in cigarette smokers: potential implications as a biomarker of risk of prostate cancer.

Tobacco smoke may be one of the most common sources of cadmium (Cd) in the general population, particularly in the rising population of smokers in developing countries. Although a relationship between both cigarette smoking and environmental Cd contamination with prostate cancer exist, the mechanisms are unclear. Most prospective cohort studies found a positive association between current smoking and a fatal cancer of the prostate. We investigated the interaction between zinc and cadmium and the potential risk of prostate cancer in smokers. Serum cadmium level was significantly (P < 0.001) higher in smokers compared with non-smokers, the level in smokers was three-fold that in non-smokers. In contrast zinc was significantly (P < 0.001) reduced in smokers compared with non-smokers. Unlike Zn, Cu was significantly (P < 0.05) higher in smokers than in non-smokers. Iron (Fe) though higher in smokers was not significantly different. Zinc: cadmium ratio was very significantly (P < 0.001) reduced, implying high cadmium: zinc ratio. This ratio was 4.5-fold the level in non-smokers. Total protein, albumin and total globulin levels were all significantly (P < 0.001) reduced in smokers compared with non-smokers respectively. Potassium (K+) was significantly (P < 0.05) higher in smokers than in non-smokers. Magnesium (Mg) was significantly (p < 0.01) reduced in smokers compared to non-smokers. Altered Zn status culminating in high Cd:Zn ratio appears the central factor in smokers; leading to oxidative stress, DNA damage, mutation, impaired DNA repair, P53 expression, angiogenic effect of Cu and impaired vitamin A metabolism. These converge in the risk of the carcinogenic process, suggesting high Cd: Zn ratio as the critical determinant of the risk of prostate cancer in smokers and possibly a biomarker of susceptibility to this environmental disease.

Suicide attempt by hanging in preadolescent children: a case series.

BACKGROUND: Suicide is now among the five top causes of death in youth worldwide. However, during the preadolescent period, suicidal behaviour is rare and difficult to define because the cognitive level of young children limits their ability to plan and understand the consequences or the finality of suicide. There is virtually no information about preadolescent suicidal behaviour in Nigeria. OBJECTIVE: To illustrate the presentation and psychosocial issues associated with preadolescent suicidal attempt using the 'hanging' method in Nigeria. METHODS: Three case scenarios of suicide attempt by hanging in preadolescents seen at the University College Hospital, Ibadan between 2005 and 2006 were interviewed in detail along with mental state and physical examination. Family and individual therapies were embarked upon. RESULTS: Types of psychopathology found in the preadolescents include depressive symptoms, conduct and oppositional defiant disorder and impulse control problems. Stressful life events such as family disruption, physical abuse, and bullying at school were factors associated with suicidal behaviour. The influence of the media in providing information about 'hanging' as a method of suicide was evident. Therapy yield varying results. CONCLUSION: High risk parameters for suicide in children should be known to all health professionals. The importance of intervention strategies particularly media education, monitoring systems and further research on suicidal behaviour in this environment is apparent.

Pharmacists and Nurses Perception of Medication Errors in a Nigerian University Teaching Hospital

Purpose: To investigate the type and frequency of all medication dispensing and administration errors as perceived by pharmacists and nurses respectively; and the factors associated with such errors in a Nigerian university teaching hospital. Methods: The study was conducted at the Obafemi Awolowo University Teaching Hospitals; Ile-Ife and Ilesa; Nigeria. Data was collected by the use of pre-tested questionnaire administered to 35 pharmacists and a stratified sample of 130 nurses over a period of 2 weeks. The questionnaires were sorted and analysed. Results: The pharmacists that responded (80) cited incorrect drug; incorrect strength of drug (70) and wrong dose of drug (60) as the most common dispensing errors. Fifty percent of pharmacists put the estimated frequency of occurrence of these dispensing errors at 1 per 100 prescriptions dispensed. Most of the nurses (65) identified administration of wrong drug; administration of wrong dose (63) and wrong time of drug administration (57) as the most frequently occurring medication administration errors in the teaching hospital. All the pharmacists and 78of nurses identified excess workload as the most important factor contributing to errors in medication dispensing and administration respectively. Conclusion: The fundamental factor perceived to be contributing to errors in medication dispensing and administration was excess workload resulting from insufficient members of staff

Pharmacists and Nurses Perception of Medication Errors in a Nigerian University Teaching Hospital

Purpose: To investigate the type and frequency of all medication dispensing and administration errors as perceived by pharmacists and nurses respectively; and the factors associated with such errors in a Nigerian university teaching hospital. Methods: The study was conducted at the Obafemi Awolowo University Teaching Hospitals; Ile-Ife and Ilesa; Nigeria. Data was collected by the use of pre-tested questionnaire administered to 35 pharmacists and a stratified sample of 130 nurses over a period of 2 weeks. The questionnaires were sorted and analysed. Results: The pharmacists that responded (80) cited incorrect drug; incorrect strength of drug (70) and wrong dose of drug (60) as the most common dispensing errors. Fifty percent of pharmacists put the estimated frequency of occurrence of these dispensing errors at 1 per 100 prescriptions dispensed. Most of the nurses (65) identified administration of wrong drug; administration of wrong dose (63) and wrong time of drug administration (57) as the most frequently occurring medication administration errors in the teaching hospital. All the pharmacists and 78of nurses identified excess workload as the most important factor contributing to errors in medication dispensing and administration respectively. Conclusion: The fundamental factor perceived to be contributing to errors in medication dispensing and administration was excess workload resulting from insufficient members of staff.) cited incorrect drug; incorrect strength of drug (70) and wrong dose of drug (60) as the most common dispensing errors. Fifty percent of pharmacists put the estimated frequency of occurrence of these dispensing errors at 1 per 100 prescriptions dispensed. Most of the nurses (65) identified administration of wrong drug; administration of wrong dose (63) and wrong time of drug administration (57) as the most frequently occurring medication administration errors in the teaching hospital. All the pharmacists and 78of nurses identified excess workload as the most important factor contributing to errors in medication dispensing and administration respectively. Conclusion: The fundamen- tal factor perceived to be contributing to errors in medication dispensing and administration was excess workload resulting from insufficient members of staff

Decreased thiamine and magnesium levels in the potentiation of the neurotoxicity of lead in occupational lead exposure.

The relationship between blood lead (Pb) and serum levels of calcium and of neural nutrients such as thiamine and magnesium (Mg) has been determined in a Nigerian population that is occupationally exposed to Pb. Forty-seven male Pb workers were recruited as test subjects and 25 males unexposed to Pb served as controls. The test subjects were classified into three groups, based on severity of exposure to Pb. Blood lead (BPb) and the serum levels of Mg, thiamine, and calcium were determined in both test subjects and controls. The mean blood Pb level was not significantly higher in Pb workers. In contrast, Mg and thiamine levels were significantly decreased (p<0.05; p<0.01, respectively). However, the calcium level was not significantly lower in test subjects than in controls. Also, there was a significant negative correlation between serum thiamine and blood Pb levels (r=-0.50; p<0.01). Furthermore, there was a significant negative correlation between serum calcium and BPb levels (r=-0.41; p<0.01). This study has shown that relatively low BPb levels can enhance Pb absorption and also potentiate Pb neurotoxicity in the presence of decreased serum thiamine and Mg levels.

Selected heavy metals in blood of male Nigerian smokers.

Lead, cadmium and zinc levels were determined in the blood of 47 all male smokers (age range, 19-56 years). The subjects were classified into 4 subgroups based on the number of years of smoking, namely: 1-5, 6-10, 11-15 years, >16 years. Thirty all male non-smokers serves as the control. The weight, height and body mass indexes of all subjects were noted in addition to other information obtained through structured questionnaire. Those that were likely to be exposed to any of these metals either occupationally or through other life style habits were excluded from the study. The mean values of blood lead (BPb), blood cadmium (BCd) and blood zinc (BZn) in male smokers (n=47) were 43.26 +/- 4.28, 98.21 +/- 6.39 and 104.84 +/- 3.66 microg dL(-1), respectively while the corresponding mean values for these parameters in the non smokers (n = 30) were 32.44 +/- 3.02, 76.51 +/- 4.59 and 90.38 +/- 2.55 microg dL(-1), respectively. The smokers have statistically significant higher BPb, BCd and BZn than the non-smokers (p < 0.001, p < 0.001 and p < 0.001, respectively). The results appear to confirm the presence of these metals as component of cigarette and agree with similar findings from literature. Consequently, it is probable that high level of these metals in blood of smokers is from tobacco smoking. Additionally it appears that the number of years of smoking has nothing to do with the level of these metals in smokers' blood.

Lead levels in some biological samples of auto-mechanics in Abeokuta, Nigeria.

Lead levels were determined in the blood, scalp hair and fingernails of 38, all male auto-mechanics (aged 18-45 years) from Abeokuta, South-western Nigeria. The subjects were classified into four sub-groups based on the period of exposure namely: 1-5, 6-10, 11-15, and >16 years. Thirty-two occupationally unexposed subjects (mainly office workers) served as the control. The weight, height and body mass indexes of all subjects were noted, in addition to other information obtained through structured questionnaire. The mean values of blood lead (BPb), hair lead (HPb) and fingernail lead (NPb) of the occupationally exposed subjects (n=38) were 48.50 +/- 9.08 microg/dL, 17.75 +/- 5.16 microg/g, and 5.92 +/- 3.30 microg/g respectively, while the corresponding mean values for these parameters in the control subjects (n = 32) were 33.(,5 +/- 10.09 microg/dL, 14.30 +/- 5.90 microg/g and 5.31 +/- 2.77 microg/g respectively. The differences in BPb and HPb levels of the two groups were statistically significant (P <0.05 and P <0.01 respectively), while that of NPb was not significant. The levels of lead in the biological samples appeared to have no relationship with the number of years on the job. From these results, it was obvious that the higher levels of lead in the biological samples of test subjects, compared with those of the controls were from environmental sources.

Amelioration of carbon tetrachloride-induced hepatotoxicity by terpenoid extract from leaves of Vernonia amydgalina.

Sesquiterpene lactone extract from the leaves of Vernonia amygdalina was tested for antihepatotoxic activity. Adult male rats were selected for the study. One group of rats was treated with toxic doses of carbon tetrachloride (CCl4) the second group was pretreated with known concentration of terpenoid extract from leaves of V. amygdalina. One hour prior to receiving toxic doses of CCl4, Kolaviron, a biflavonoid extract of the seeds of Garcina kola was used as a positive control. Serum enzymes, alanine amino transferase (ALT), ornithine carbamoyl transferase (OCT) that are known to be very sensitive to cytotoxic hepatic injury, and aspertate amino transferase (AST) that is particularly sensitive to carbon tetrachloride poisoning, were measured as indices of hepatotoxicity. The results obtained showed that there were reduction in the activities of serum ALT, AST and OCT from 20.57 +/- 5.59, 10.46 +/- 6.71 and 184.8 +/- 10.45 in animals treated with toxic doses of CCl4 to 3.40 +/- 0.10, 3.95 +/- 0.15 and 1293 +/- 12.10 in animal pretreated with terpenoid extract before CCl4 intoxication, representing 83.5%, 62.3%, and 30% decrease respectively. These decreases were statistically significant (P < 0.001, P < 0.05, P < 0.001 respectively). From these results, it is concluded that sesquiterpene lactone extract from the leaves of V. amygdalina like kolaviron, a biflavonoid extract from the seeds of G. kola has antihepatotoxic activity in CCl4-induced hepatic damage in rats.

Abnormal mucociliary action in asthma and bronchiectasis.

Nasal mucociliary clearance (NMCC) time was measured in four groups of patients: asthmatics with allergic rhinitis, asthmatics without rhinitis, bronchiectasis and normal subjects. The saccharin method was used for the study. The NMCC time was prolonged significantly in the asthmatic groups and group with bronchiectasis when compared with control subjects (P less than 0.001). It is likely that the impaired mucociliary clearance is due to a combination of mucus abnormality and ciliary malfunction.

Subgingival occurrence of enteric rods, yeasts and staphylococci after systemic doxycycline therapy.

The occurrence of subgingival enteric rods, yeasts and staphylococci (pre-treatment and post-treatment) was examined in 21 adult periodontitis patients receiving mechanical periodontal therapy and systemic doxycycline (200 mg on first day, then 100 mg/day for 20 days). Subgingival samples obtained prior to, and 2 weeks and 4 weeks after completion of the antibiotic therapy were transported in VMGA III and plated onto TSBV (for enteric rods and yeasts) and Staphylococcus 110 media. At baseline, small numbers of enteric rods (3 patients), yeasts (5 patients) and staphylococci (11 patients) were detected subgingivally. After the antibiotic therapy, more than 10-fold increases were seen in subgingival numbers of Enterobacter aerogenes (2 patients), Escherichia coli (1 patient), Candida albicans (2 patients), and staphylococci (11 patients). These findings demonstrating that systemic doxycycline therapy may result in at least temporary subgingival overgrowths of enteric rods, yeasts and staphylococci, may indicate the risk of frequent use of tetracycline in periodontal therapy.