[Proliferation of fat cells in lipomas]. / O proliferatsii zhirovykh kletok v lipomakh.

Common lipomas and destructive ones (intramuscular, infiltrating, necrotizing and destroying surrounding tissues) were studied in 9 patients electron-microscopically and radioautographically. The adequacy of necrotizing processes and regeneration was observed: normal fat tissue-lipoma-destructive lipoma. In the latter besides pronounced destruction of tissue (adipocytes, vessels, muscles at the border with tumour), there was accelerated differentiation of preadipocytes from the cells-precursors (fibroblasts and undifferentiated cells of the vascular wall), hypertrophy of preadipocytes (their size and number of nuclei increase) and their proliferation. The process of malignant transformation in a destructive lipoma may be so much pronounced that DNA synthesis may be observed in the mature lipocyte (this has not been described so far).

[Efficacy of clinical-serologic diagnosis of wound infection in burned patients]. / Izuchenie éffektivnosti kliniko-serologicheskoi diagnostiki ranevoi infektsii u obozhzhennykh.

Titer of antibodies to microorganisms obtained from wound and blood of 46 patients with burns of IIIAB--IV degree was studied. The majority of the burned patients have ability to synthesize antibodies. Agglutination of the bacteria by antibodies promoted location of infectious agent in primary focus and impeded bacteria to spread into blood and generalization of process. Not all microorganisms of wound provoke antibodies synthesis, i.e. wound infection. Therefore bacteriologic study must be supplemented by serum diagnosis. Detection of bacteria in the blood without serologic data do not permit to consider these bacteria as etiologic factor of wound infection.

[Immune status in diabetic patients with pyo-necrotic lesions of lower extremities]. / Sostoianie immuniteta u bol'nykh sakharnym diabetom s gnoino-nekroticheskim porazheniem nizhnikh konechnostei.

Long-term and severe pyonecrotic processes in diabetic patients testify to severe disorders of immune system in this disease. High titer of antibodies to tested autostrain demonstrated its etiologic role in infectious process. The study group consisted of 29 patients (with diabetic pyonecrotic foot lesions), control group--17 patients with burns of III a, b--IV stage affecting from 20 to 60% of body surface. In diabetic patients antibodies titer to the most encountered infectious agents Staphilococcus aureus and Pseudomonas aeroginosa was lower than in burn patients with immunity deficiency. Decrease of antibodies titer in diabetic patients testifies to high insufficiency of B-immunity.

Morphological changes in burn wounds after transplantation of allogenic fibroblasts.

Cultured allogenic fibroblasts labeled with fluorescent acridine orange and transplanted onto burn wound virtually completely disappear from the wound surface within 2 days (are destroyed). Study of wound morphology by light autoradiography and quantitative analysis showed a stimulating effect of transplanted fibroblasts on wound healing. This effect, irrespective of the initial state of the wound, is always directed at accelerated formation of connective tissue similar to the derma and capable of epithelialization.

[Morphological examinations of infected burn wounds]. / Morfologicheskoe izuchenie infitsirovaniia ozhogovykh ran.

Electron microscopical and electron radioautographic methods have been used for morphological examination. This allowed to raise sensitivity of the tests facilitating detection of living solitary bacterial cells (by intensive mark above them). Radioautography showed functional status of wound bacteria (the intensity of the metabolism and reproduction). It was determined that in living tissues, not damaged by the burn and unchanged morphologically bacteria do not penetrate. Especially massive accumulations of the microorganisms are found in necrotic tissues, under the scab, at the external border of demarcation protuberance, and they represent colonization of the wound. When modern methods of treatment and early necrectomy are used, morphological examination usually does not reveal pathogenic microorganisms. Only inflammatory reaction of the wound tissues could be seen: oedema, hyperemia, hemorrhages, cellular infiltrates. The development of granulation tissue indicated cessation of wound infection. Especially favourable feature, demonstrating healing of the wound, was appearance and reproduction of young cells of fat tissue (indusion of labelled thymidine). The results of the study have confirmed the advantage of new electronic methods for microscopy in infected burn wounds.

[A morphological and electron radioautographic study of the wound in the diabetic foot syndrome]. / Morfologicheskoe i élektronno-radioavtograficheskoe izuchenie rany pri sindrome diabeticheskoi stopy.

Two groups of patients with a grave form of diabetes mellitus type I and II with diabetic foot syndrome have been examined. Group 1 (21 cases) consisted of patients whose foot was amputated. The cause of the necrotic process was ischemia produced by alteration of the arterial vessels aggravated by infection. Patients of group 2 (18 cases) were treated with resultant wound healing and foot preservation. The material was subdivided into two subgroups: 1) tissue from the "infectious" foot with great amount of bacteria and inflammation reaction; 2) tissue from the "ischemic" foot with considerable alterations of microvessels structure. In both groups well developed granulation tissue was observed after the treatment; almost all the cells of this tissue included 3H-uridin and labelling with 3H-thymidine was increased in fibroblasts, endotheliocytes and pericytes.

[The causative agent of wound infection and sepsis in burn patients]. / Vozbuditel' ranevoi infektsii i sepsisa u obozhzhennykh.

The content of antibodies to the autostrains of bacteria isolated from the wound and blood of burn patients was studied. Antibodies were found not to all species and strains isolated from the wound, but only to 1 or 2 of them. Usually Staphylococcus aureus and Pseudomonas were detected. The appearance or sharp rise of the titer of antibodies even to one of all strains contaminating the wound was observed in all examined burn patients, including those having septic complications, i.e. all these patients retained their capacity of antibody formation. The causative agent of wound infection and sepsis, determined by a high titer of antibodies to it, was seldom detected in the blood. This was due to the fact that bacteria were agglutinated by antibodies or retained in tissues on the level of the histo-hematic barrier. In cases of the development of septic endocarditis the inhibiting role of antibodies was not manifested, and the causative agent of this infection was often detected in the blood.

[The effect of antibodies to microorganisms in a focus of purulent inflammation]. / Vliianie antitel na mikroorganizmy v ochage gnoinogo vospaleniia.

The rats were infected with a suspension of dissociated or agglutinated Pseudomonas aeruginosa [correction of B. pynocyaneus]. Infection with the agglutinated agent drastically improved the clinical course, lowered the indices of the process generalization (mortality, metastatic foci, spread in the internal organs); no hemorrhages, necrosis or abscesses were noted. A rapid (within the first day) death of almost all agglutinated bacteria in the primary focus was observed, this not being due to phagocytosis or complement-dependent lysis.

[Inhibitory effect of antimicrobial antibodies on the generalization of wound infection]. / O tormoziashchem deistvii protivomikrobnykh antitel na generalizatsiiu ranevoi infektsii.

The antibody titer in wound and burn patients has been subjected to agglutination test to reveal bacterial autostrains. The antibodies to blood--isolated autostrains were shown to be present in lower titer, but more often they weren't found at all. At the clinical manifestation of sepsis the pathogenic strain (uniformly present in wound and secondary focuses, agglutinated by patient's serum in higher titer) hasn't been detected in blood. The authors conclude that the bacterial invasion of blood and generalization of infection occurs in the very first days following the emergence of bacteria in primary focus, when the antibodies to these bacteria haven't arisen yet and these is no clinical evidence of sepsis due to the small amount of those bacteria in primary focus. At the clinical manifestation of sepsis there is scant likelihood that the pathogen might be isolated from blood, occasional strains with no antibodies, may, however, be encountered.

[Electron-radioautographic study of the action of "levosin" ointment and the ointment base (polyethylene oxide) on Staphylococcus aureus]. / Elektronno-radioavtograficheskoe issledovanie deistviia mazi "Levosin" i mazevoi osnovy--poliétilenoksida na zolotistyi stafilokokk.

Morphological properties and intensity of RNA synthesis in the Staphylococcus aureus cells exposed to levosin ointment or it base alone (a mixture of polyethylene glycols) were studied with electron microscopy. It was found that the exposure to the substances led to various destructions in the microbial cells, marked inhibition of their viability evident from lower levels of RNA synthesis by the bacteria and a decrease in the count of the bacterial cells. There were certain differences in the effect of the substances on the morphological properties of the bacteria, while the level of their viability inhibition was the same.

[Role of bacterial agglutination in wound infection]. / Rol' aggliutinatsii bakterii v ranevoi infektsii.

Method of study of bacterial agglutination by means of serum taken from patients with wound infections was suggested. It was found out that agglutination suppresses viability of microbial cells in the center of agglutinate and reduces the risk of bacterial spreading from the wound over the body.

[An electron microscopic radioautographic study of the viability of human cells after death. The postmortem activation of RNA synthesis in neutrophils]. / Elektronno-radioavtograficheskoe issledovanie zhiznesposobnosti kletok cheloveka posle smerta. Posmertnaia aktivatsiia sinteza RNK v neitrofilakh.

RNA synthesis in human neutrophils isolated from blood 2.5-67 hours after death of patients was studied by means of light and electron microscopy radioautography. The rate of RNA synthesis in neutrophils isolated from blood of cadavers was higher in comparison with the same parameter in neutrophils isolated from survivor's blood. The significance of this observation for resuscitation and transplantology is discussed.