ABSTRACT
Atomic force microscopy (AFM) was employed to study certain enzyme immunoassay steps for the detection of hepatitis B surface antigen (HBsAg). Physical adsorption of monoclonal antibodies (mAb), blocking of surface active sites free of antibodies by neutral proteins, and capture of HBsAg particles by sensitized surfaces were visualized successively in microplate wells of standard immunological plates from various manufacturers. The previously undescribed details such as "etching holes" up to 20 nm in depth were observed on the surface of plates some companies. The quantitative relationships between the optical density (OD) values obtained by enzyme immunoassay (EIA) and the number of antigen-antibody complexes in AFM were calculated.
Subject(s)
Hepatitis B Surface Antigens/analysis , Microscopy, Atomic Force , Adsorption , Antigen-Antibody Reactions , Enzyme-Linked Immunosorbent Assay , Hepatitis B Surface Antigens/immunology , Particle Size , Protein Denaturation , Protein Structure, Tertiary , Surface Properties , TemperatureABSTRACT
Under supervision there were 38 men (age from 36 till 57 years) with diffuse hepatic diseases (DHD) in stadia of hepatitis, 27--in dynamics of treatment of essential phospholipids. Is experimentally established: structure-functional erythrocyte characteristics by methods of dielectrophoresis, thin-layer chromatography correlate with lipid and phospholipid composition of red cell membranes. The low level of phospholipids in erythrocyte membranes and direction of changes in their fractional composition in the DHD is the basis for the use of essential phospholipids. Against the background of therapy noted a significant increase in amplitude of deformation, polarizability, capacity, speed of movement of red blood cells to the electrodes and lower levels of generalized indicators of viscosity, rigidity, electrical conductivity, index of aggregation and destruction.
Subject(s)
Adjuvants, Immunologic/administration & dosage , Erythrocyte Deformability/drug effects , Erythrocyte Membrane/metabolism , Hepatitis, Chronic/drug therapy , Hepatitis, Chronic/metabolism , Phosphatidylcholines/administration & dosage , Phosphatidylcholines/metabolism , Adjuvants, Immunologic/metabolism , Adult , Electric Impedance , Female , Humans , Male , Middle Aged , ViscosityABSTRACT
Spectra of amplitudes of polarization of erythrocytes, yeast cells, and latex particles in the range of 1-10 Hz were investigated by the method of dielectrophoresis. Positive dielectrophoresis of erythrocytes and yeast cells the frequency range of 60 - 100 Hz was revealed. The theoretically positive dielectrophoresis was evidenced by the occurrence of channels across the cell membrane and bacterial cell wall.