ABSTRACT
To meet the increasing needs of point-of-care testing in clinical diagnosis and daily health monitoring, numerous cutting-edge techniques have emerged to upgrade current portable biosensors with higher sensitivity, smaller size, and better intelligence. In particular, due to the controlled locomotion characteristics in the micro/nano scale, microrobots can effectively enhance the sensitivity of biosensors by disrupting conventional passive diffusion into an active enrichment during the test. In addition, microrobots are ideal to create biosensors with functions of on-demand delivery, transportation, and multi-objective detections with the capability of actively controlled motion. In this review, five types of portable biosensors and their integration with microrobots are critically introduced. Microrobots can enhance the detection signal in fluorescence intensity and surface-enhanced Raman scattering detection via the active enrichment. The existence and quantity of detection substances also affect the motion state of microrobots for the locomotion-based detection. In addition, microrobots realize the indirect detection of the bio-molecules by functionalizing their surfaces in the electrochemical current and electrochemical impedance spectroscopy detections. We pay a special focus on the roles of microrobots with active locomotion to enhance the detection performance of portable sensors. At last, perspectives and future trends of microrobots in biosensing are also discussed.
ABSTRACT
Autonomous movement promotes practical applications of micromotors. Understanding the moving speeds is a crucial step in micromotor studies. The current analysis method relies on an expensive optical microscope, which is limited to laboratory settings. Herein, we have developed a lightweight (0.15 g), portable (2.0 × 3.5 cm2), and low-cost (approximately $0.26) micromotor sensor (µ-Motor sensor), composed of water-sensitive materials for micromotor speed monitoring. Moving micromotors induce fluid flow, enhancing the evaporation rate of the liquid medium. Consequently, a high correlation between motor speed and water molecule concentration above the moving medium has been established. The µ-Motor sensor enables a real-time readout of the moving speed in various settings, with high accuracy (≥95% in the lab and ≥90% in field studies at a local beach). The µ-Motor sensor opens up a new way for detecting micro/nanomachine movements, illuminating future applications of micro/nanorobotics for diverse scenarios.
