ABSTRACT
INTRODUCTION: AIM2 inflammasome activation leads to the release of IL-ß, which plays an important role in rheumatoid arthritis pathogenesis. In this work, we evaluated AIM2 expression and activity in RA patients and healthy controls. METHODS: AIM2 and RANKL expression were evaluated by flow cytometry. Inflammasome activity was determined in monocyte cultures stimulated with synthetic DNA by measuring IL-1ß levels in supernatants using an ELISA assay. The caspase-1 expression in monocytes was measured by western blot, the POP3 expression was analysed by qPCR, and serum levels of IFN-γ were evaluated using ELISA assay. RESULTS: We observed a diminution of CD14+AIM2+ cells in RA patients, associated with disease activity and evolution. Likewise, the levels of IL-1ß were increased in monocyte cultures un-stimulated and stimulated with LPS from RA patients with DAS28 ≥ 4. The Caspase-1 activity and RANKL + monocytes in RA patients were slightly increased. Finally, augmented POP3 expression and diminished IFN-γ serum levels were detected in RA patients. CONCLUSION: Our results showed that the monocytes from RA patients were prone to release IL-1ß in the absence of the AIM2 inflammasome signal. The down-regulation of AIM2 to a systemic level in RA patients might be a consequence of augmented POP3 expression and might imply the survival of pro-inflammatory cells contributing to the inflammation process.
Subject(s)
Arthritis, Rheumatoid/metabolism , DNA-Binding Proteins/metabolism , Inflammasomes/metabolism , Adult , Caspase 1/metabolism , Cells, Cultured , Female , Humans , Inflammation/metabolism , Interferon-gamma/metabolism , Interleukin-1beta/metabolism , Lipopolysaccharide Receptors/metabolism , Male , Monocytes/metabolism , mTOR Associated Protein, LST8 Homolog/metabolismABSTRACT
We assessed the possible association between several single nucleotide polymorphisms (SNP) of P2RX7 gene with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). We determined the function of P2X7 receptor and the frequency of the 489C>T, 1096C>G, and 1513A>C SNP of P2RX7 gene in 111 and 122 patients with SLE and RA, and 98 healthy subjects. We found no significant association between the SNPs studied and SLE or RA. We also detected that lymphocytes from SLE and RA patients with the 489C>T SNP showed a higher ethidium bromide uptake in response to ATP than wild type or 1096C>G/1513A>C subjects. In addition, cells from RA patients and the 489C>T genotype, showed higher [Ca(2+)]i responses to ATP. Our data indicate that the 489C>T SNP of P2RX7 gene confers an enhanced function of this receptor in patients with RA, which may contribute to the pathogenesis of this condition.
Subject(s)
Arthritis, Rheumatoid/genetics , Arthritis, Rheumatoid/immunology , Polymorphism, Single Nucleotide , Receptors, Purinergic P2X7/genetics , Receptors, Purinergic P2X7/immunology , Adenosine Triphosphate/immunology , Adenosine Triphosphate/metabolism , Adolescent , Adult , Alleles , Calcium/immunology , Calcium/metabolism , Cells, Cultured , Female , Genotype , Humans , Interleukin-18/immunology , Interleukin-1beta/immunology , Lupus Erythematosus, Systemic/genetics , Lupus Erythematosus, Systemic/immunology , Male , Middle Aged , Young AdultABSTRACT
Las moléculas de adhesión celular tienen un papel importante en múltiples fenómenos biológicos, tanto normales (generación de la respuesta inmune, coagulación, cicatrización, órgano-génesis) como patológicos (inflamación, trombosis, metástasis de células tumorales). En el presente trabajo se revisan los aspectos básicos de las moléculas de adhesión celular y su papel en diversas condiciones patológicas que afectan a la piel
Subject(s)
Epidermis/cytology , Epidermis/immunology , Epidermis/physiology , Inflammation/physiopathology , Inflammation/immunology , Integrins/physiology , Cell Adhesion Molecules/classification , Cell Adhesion Molecules/physiology , Cell Adhesion Molecules/immunology , Neoplasm Metastasis , Skin Diseases/immunology , Skin/cytology , Skin/physiology , Structure-Activity RelationshipABSTRACT
Se presenta el caso de un paciente con dermatitis por contacto con autoeccematización. Se hace referencia a la inmunopatogenia de esta condición, la cual parece consistir en un fenómeno de autoinmunidad de tipo celular hacia antígenos epidérmicos autólogos
Subject(s)
Aged , Humans , Male , Administration, Topical , Autoimmunity/immunology , Betamethasone/administration & dosage , Dermatitis, Allergic Contact/diagnosis , Dermatitis, Allergic Contact/immunology , T-LymphocytesABSTRACT
El herpes zoster es una infección aguda, causada por el virus de la varicela zoster (VZV), que se manifiesta clínicamente por la aparición de vesículas umbilicadas, limitadas a un dermatoma, acompañándose de dolor y en ocasiones de malestar general. Se describen dos casos clínicos de herpes zoster con presentaciones atípicas. El reconocimiento temprano de las lesiones es necesario para tratar de evitar complicaciones del tipo de la neuralgia posherpética o bien la cicatrización residual
Subject(s)
Adult , Aged , Humans , Female , Acyclovir/administration & dosage , Skin Diseases, Viral/diagnosis , Skin Diseases, Viral/physiopathology , Herpes Zoster/physiopathologyABSTRACT
El síndrome de Sweet (SS) ha sido ocasionado con neoplasias hematológicos y en menor grado con tumores sólidos; la patogénesis aún no se ha dilucidado, sin embargo, hay evidencias a favor de mecanismos inmunopatogénicos. Se presenta un caso clínico de un paciente con SS y se revisan los factores que actualmente han sido involucrados en su patogenia