Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Int J Biol Macromol ; 167: 267-278, 2021 Jan 15.
Article in English | MEDLINE | ID: mdl-33242552

ABSTRACT

This study aims to examine whether two L-amino acid oxidases isolated from Bothrops snake venom (SV-LAAOs) were cytotoxic to Leishmania (Leishmania) amazonensis and Leishmania (Viannia) braziliensis, two causative agents of leishmaniasis, which is an endemic disease in tropical and subtropical countries. The SV-LAAOs BjussuLAAO-II and BmooLAAO-II were isolated from Bothrops jararacussu and Bothrops moojeni venom, respectively, through a three-step chromatography process that used molecular exclusion, hydrophobic interaction, and affinity columns. BmooLAAO-II is a new SV-LAAO isoform that we isolated in this study. The purified BjussuLAAO-II and BmooLAAO-II had high L-amino acid oxidase-specific activity: 3481.17 and 4924.77 U/mg/min, respectively. Both SV-LAAOs were strongly cytotoxic to the two Leishmania species, even at low concentrations. At the same concentration, BjussuLAAO-II and BmooLAAO-II exerted different cytotoxic effects on the parasites. We reported for the first time that the SV-LAAOs suppressed cell proliferation and altered the mitochondrial membrane potential of the two Leishmania species. Surprisingly, BjussuLAAO-II increased the intracellular reactive oxygen species production only in L. (L.) amazonensis, while BmooLAAO-II increased the intracellular reactive oxygen species production only in L. (V.) braziliensis, indicating that these SV-LAAOs had a certain specificity of action.


Subject(s)
Antiprotozoal Agents/isolation & purification , Antiprotozoal Agents/pharmacology , Bothrops , Crotalid Venoms/enzymology , L-Amino Acid Oxidase/isolation & purification , L-Amino Acid Oxidase/pharmacology , Leishmania/drug effects , Amino Acid Sequence , Animals , Brazil , Chromatography , Enzyme Activation , L-Amino Acid Oxidase/chemistry , Membrane Potential, Mitochondrial/drug effects , Mitochondria/drug effects , Mitochondria/genetics , Mitochondria/metabolism , Parasitic Sensitivity Tests , Reactive Oxygen Species/metabolism
SELECTION OF CITATIONS
SEARCH DETAIL
...