ABSTRACT
Microbial Fuel Cells (MFCs) transform organic matter into electricity through microbial electrochemical reactions catalysed on anodic and cathodic half-cells. Terrestrial MFCs (TMFCs) are a bioelectrochemical system for bioelectricity production as well as soil remediation. In TMFCs, the soil is the ion-exchange electrolyte, whereas a biofilm on the anode oxidises organic matter through electroactive bacteria. Little is known of the overall microbial community composition in a TMFC, which impedes complete exploitation of the potential to generate energy in different soil types. In this context, an experiment was performed to reveal the prokaryotic community structure in single chamber TMFCs with soil in the presence and absence of a municipal waste compost (3% w/v). The microbial community was assessed on the anode and cathode and in bulk soil at the end of the experiment (54 days). Moreover, TMFC electrical performance (voltage and power) was also evaluated over the experimental period, varying the external resistance to improve performance. Compost stimulated soil microbial activity, in line with a general increase in voltage and power. Significant differences were observed in the microbial communities between initial soil conditions and TMFCs, and between the anode, cathode and bulk soil in the presence of the compost. Several electroactive genera (Bacillus, Fulvivirga, Burkholdeira and Geobacter) were found at the anode in the presence of compost. Overall, the use of municipal waste compost significantly increased the performance of the MFCs in terms of electrical power and voltage generated, not least thanks to the selective pressure towards electroactive bacteria on the anode.
Subject(s)
Bioelectric Energy Sources , Composting , Bioelectric Energy Sources/microbiology , Electricity , Bacteria , Electrodes , SoilABSTRACT
The Aliivibrio fischeri bioassay was successfully applied in order to evaluate the acute effect of sulfamethoxazole (SMX), ciprofloxacin (CIP), chlortetracycline (CTC) and copper (Cu), alone or in binary, ternary, and overall mixture. The toxicity results are reported in terms of both effective concentrations, which inhibited 50% of the bacterium bioluminescence (EC50%), and in Toxic Units (TUs). The TUs were compared with predicted values obtained using the Concentration Addition model (CA). Finally, the toxicity of water extracts from a soil contaminated by the three antibiotics (7 mg Kg-1 each) in the presence/absence of copper (30 mg Kg-1) was also evaluated. Copper was the most toxic chemical (EC50: 0.78 mg L-1), followed by CTC (EC50: 3.64 mg L-1), CIP (96 mg L-1) and SMX (196 mg L-1). Comparing the TU and CA values of the mixtures, additive effects were generally found. However, a synergic action was recorded in the case of the CIP+Cu co-presence and antagonistic effects in the case of CTC+Cu and the ternary mixture (containing each antibiotic at 0.7 mg L-1), were identified. Soil water extracts did not show any toxicity, demonstrating the buffering ability of the soil to immobilize these chemicals.
Subject(s)
Chlortetracycline , Water Pollutants, Chemical , Aliivibrio fischeri , Anti-Bacterial Agents/toxicity , Biological Assay , Chlortetracycline/toxicity , Copper/toxicity , Soil , Sulfamethoxazole/toxicity , Water , Water Pollutants, Chemical/toxicity , Water Pollutants, Chemical/analysisABSTRACT
Antibiotics (ABs) have made it possible to treat bacterial infections, which were in the past untreatable and consequently fatal. Regrettably, their use and abuse among humans and livestock led to antibiotic resistance, which has made them ineffective in many cases. The spread of antibiotic resistance genes (ARGs) and bacteria is not limited to nosocomial environments, but also involves water and soil ecosystems. The environmental presence of ABs and ARGs is a hot topic, and their direct and indirect effects, are still not well known or clarified. A particular concern is the presence of antibiotics in agroecosystems due to the application of agro-zootechnical waste (e.g., manure and biosolids), which can introduce antibiotic residues and ARGs to soils. This review provides an insight of recent findings of AB direct and indirect effects on terrestrial organisms, focusing on plant and invertebrates. Possible changing in viability and organism growth, AB bioaccumulation, and shifts in associated microbiome composition are reported. Oxidative stress responses of plants (such as reactive oxygen species production) to antibiotics are also described.
ABSTRACT
Electroactive bacteria (EAB) are natural microorganisms (mainly Bacteria and Archaea) living in various habitats (e.g., water, soil, sediment), including extreme ones, which can interact electrically each other and/or with their extracellular environments. There has been an increased interest in recent years in EAB because they can generate an electrical current in microbial fuel cells (MFCs). MFCs rely on microorganisms able to oxidize organic matter and transfer electrons to an anode. The latter electrons flow, through an external circuit, to a cathode where they react with protons and oxygen. Any source of biodegradable organic matter can be used by EAB for power generation. The plasticity of electroactive bacteria in exploiting different carbon sources makes MFCs a green technology for renewable bioelectricity generation from wastewater rich in organic carbon. This paper reports the most recent applications of this promising technology for water, wastewater, soil, and sediment recovery. The performance of MFCs in terms of electrical measurements (e.g., electric power), the extracellular electron transfer mechanisms by EAB, and MFC studies aimed at heavy metal and organic contaminant bioremediationF are all described and discussed.
ABSTRACT
The degradation and bioaccumulation of selected antibiotics such as the sulfonamide sulfamethoxazole (SMX) and the fluoroquinolones enrofloxacin (ENR) and ciprofloxacin (CIP) were investigated in soil microcosm experiments where Lactuca sativa was grown with manure or digestate (1%) and spiked with a mixture of the three antibiotics (7.5 mg/kg each). The soil, rhizosphere and leaf phyllosphere were sampled (at 0 and 46 days) from each microcosm to analyze the antibiotic concentrations, main resistance genes (sul1, sul2, qnrS, aac-(6')-Ib-crand qepA), the intI1and tnpA mobile genetic elements and the microbial community structure.Overall results showed that SMX and CIP decreased (70-85% and 55-79%, respectively), and ENR was quite persistent during the 46-day experiment. In plant presence, CIP and ENR were partially up-taken from soil to plant. In fact the bioaccumulation factors were > 1, with higher values in manure than digestate amended soils. The most abundant gene in soil was sul2 in digestate- and aac-(6')-Ib-cr in the manure-amended microcosms. In soil, neither sulfamethoxazole-resistance (sul1 and sul2), nor fluoroquinolone-resistance (aac-(6')-Ib-cr, qepA and qnrS) gene abundances were correlated with any antibiotic concentration. On the contrary, in lettuce leaves, the aac-(6')-Ib-cr gene was the most abundant, in accordance with the fluoroquinolone bioaccumulation. Finally, digestate stimulated a higher soil microbial biodiversity, introducing and promoting more bacterial genera associated with antibiotic degradation and involved in soil fertility and decreased fluoroquinolone bioaccumulation.
Subject(s)
Manure , Microbiota , Cattle , Animals , Manure/microbiology , Soil/chemistry , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry , Lactuca , Bioaccumulation , Ciprofloxacin , Fluoroquinolones/pharmacology , Sulfamethoxazole/chemistry , Enrofloxacin , Soil Microbiology , Fertilization , Genes, BacterialABSTRACT
Some livestock farms rely on anaerobic digestion (AD) technology for manure disposal, thus obtaining energy (biogas) and fertilizer (digestate). Mixtures of antibiotics used for animal health often occur in organic waste and their possible synergistic/antagonistic effects on microorganisms involved in AD are still poorly studied. This work focuses on the effects of adding ciprofloxacin, alone (5 mg L-1) and in combination with sulfamethoxazole (2.5-5-10 mg L-1), on AD efficiency and microbial community structure. The experiment consisted of 90-day cattle manure batch tests and antibiotic removal percentages were assessed. Adding antibiotics always promoted CH4 and H2 production compared to untreated controls; however, CH4 production was lowered with the highest ciprofloxacin (CIP) concentrations. The overall results show antibiotic degradation caused by acidogenic Bacteria, and CH4 was mainly produced through the hydrogenotrophic-pathway by methanogenic Archaea. Shifts in microbial community abundance (DAPI counts) and composition (Illumina-MiSeq and FISH analyses) were observed.
ABSTRACT
Current regulation on the authorization of plant protection products (PPPs) in the European Union is limited to the evaluation of ecological risks for the single active substances they contain. However, plant protection treatments in agriculture often consist of PPPs already containing more than one active substance; moreover, each cropped field receives multiple applications per year, leading to complex pesticide mixtures in the environment. Different transport processes lead to a multitude of heterogeneous and potentially toxic substances that, for example, may reach water bodies and act simultaneously on natural freshwater ecosystems. In this context, the development of methodologies and tools to manage risks of pesticides mixtures is imperative to improve the current ecological risk assessment procedures and to avoid further deterioration of ecological quality of natural resources. The present study suggests new procedures for identifying pesticide mixtures of potential concern released from agricultural crops in surface water. The approach follows the European Union regulatory context for the authorization of PPPs in the market (edge-of field risk assessment) and requires the use of Forum for the Co-ordination of pesticide fate models and their Use (FOCUS) models (Step 3 and 4) for calculating the concentrations in surface water of mixture components on a daily basis. Moreover, it uses concentration addition models to calculate the toxic potency of the pesticide mixtures released by a treated crop. To implement this procedure, we developed a simple Microsoft-Excel-based tool. We also considered two case studies (maize and apple tree), representative of Italian agricultural scenarios for annual and perennial crops. Moreover, we compared results with 3 years of monitoring data of surface water bodies of the Lombardia region (northern Italy) where the two crops are largely present. Environ Toxicol Chem 2022;41:2028-2038. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Subject(s)
Pesticides , Water Pollutants, Chemical , Agriculture , Crops, Agricultural , Ecosystem , Environmental Monitoring , Pesticides/analysis , Pesticides/toxicity , Risk Assessment , Water/chemistry , Water Pollutants, Chemical/analysis , Water Pollutants, Chemical/toxicityABSTRACT
A method to evaluate the ecological risk of chemical mixtures in water bodies is here presented. In the first phase, the approach considered routine chemical monitoring data (MEC: measured environmental concentrations) obtained from the Italian National Institute for Environmental Protection and Research, which were georeferenced to a single coordinate system for each monitoring station. The overall mixture toxicity were then evaluated for three representative aquatic organisms (algae, Daphnia, fish) using the concentration addition model to combine exposure with ecotoxicological data (from different databases). A database management system was used to facilitate the creation, organisation, and management of the large datasets of this study. The outputs were obtained as GIS-based mixture risk maps and tables (listing the toxic unit of mixtures and individual substances) useful for further analysis. The method was applied to an Italian watershed (Adda River) as a case study. In the first phase, the mixture toxicity was calculated using two scenarios: best- and worst-case; wherein the former included only those compounds that were be detected, while the latter involved also substances with concentrations below the limit of quantification. The ratio between the two scenarios indicated the range within which mixture toxicity should ideally vary. The method demonstrates that these ratios were very small when the calculated toxicity using the best case indicated a potential risk and vice versa, indicating that the worst-case scenario could not be appropriate (extremely conservative). Consequently, in the successive phase, we focused exclusively on the best-case scenario. Finally, this approach allowed the priority mixture identification (those most likely occurring in the analysed water samples), algae as the organism at the highest risk, and the substances that contributed the most to the overall mixture toxicity (terbuthylazine and s-metolachlor for algae, and chlorpyrifos and chlorpyrifos-CH3 for Daphnia and fish).
Subject(s)
Rivers , Water Pollutants, Chemical , Animals , Daphnia , Ecotoxicology , Risk Assessment , Water Pollutants, Chemical/analysis , Water Pollutants, Chemical/toxicityABSTRACT
In recent decades, the innovative practice of management and valorization of agrozootechnical waste as energy through anaerobic digestion (AD) has been rapidly growing. However, whether applying digestate to soil as biofertilizer can be a source of antibiotics (ABs) and antibiotic resistance genes (ARGs) has not been fully investigated so far. In this work the ARGs responsible for sulfamethoxazole (SMX) resistance (sul1, sul2), ciprofloxacin (CIP) resistance (qnrS, qepA, aac-(6')-Ib-cr) and the mobile genetic element intl1, together with the concentrations of the antibiotics SMX and CIP, were measured in several anaerobic digesters located in Central Italy. Based on these results, the concentrations of antibiotics and ARGs which can potentially reach soil through amendment with digestate were also estimated. The highest CIP and SMX concentrations were found during winter and spring in anaerobic digesters. The highest ARG abundances were found for the aac-(6')-Ib-cr and sul2 genes. The overall results showed that application of digestate to soil does not exclude AB contamination and spread of ARGs in agroecosystems, especially in the case of ciprofloxacin, owing to its high intrinsic persistence.
Subject(s)
Anti-Bacterial Agents , Soil , Anaerobiosis , Anti-Bacterial Agents/pharmacology , Drug Resistance, Microbial/genetics , Genes, Bacterial/genetics , Manure , SulfamethoxazoleABSTRACT
The anionic surfactant sodium lauryl ether sulfate (SLES) is the main component of most commercial foaming agents (FAs) used in the excavation of highway and railway tunnels with Earth pressure balance-tunnel boring machines (EPB-TBMs). Several hundreds of millions of tons of spoil material, consisting of soil mixed with FAs, are produced worldwide, raising the issue of their handling and safe disposal. Reducing waste production and reusing by-products are the primary objectives of the "circular economy," and in this context, the biodegradation of SLES becomes a key question in reclaiming excavated soils, especially at construction sites where SLES degradation on the spot is not possible because of lack of space for temporary spoil material storage. The aim of the present work was to apply a bacterial consortium (BC) of SLES degraders to spoil material excavated with an EPB-TBM and coming from a real construction site. For this purpose, the BC capability to accelerate SLES degradation was tested. Preliminary BC growth, degradation tests, and ecotoxicological evaluations were performed on a selected FA. Subsequently, a bioaugmentation experiment was conducted; and the microbial abundance, viability, and SLES concentrations in spoil material were evaluated over the experimental time (0.5, 3, 6, 24, 48, and 144 h). Moreover, the corresponding aqueous elutriates were extracted from all the soil samples and analyzed for SLES concentration and ecotoxicological evaluations with the bacterium Aliivibrio fischeri. The preliminary experiments showed the BC capability to grow under 14 different concentrations of the FA. The maximum BC growth rates and degradation efficiency (100%) were achieved with initial SLES concentrations of 125, 250, and 500 mg/L. The subsequent bioaugmentation of the spoil material with BC significantly (sixfold) improved the degradation time of SLES (DT50 1 day) compared with natural attenuation (DT50 6 days). In line with this result, neither SLES residues nor toxicity was recorded in the soil extracts showing the spoil material as a by-product promptly usable. The bioaugmentation with BC can be a very useful for cleaning spoil material produced in underground construction where its temporary storage (for SLES natural biodegradation) is not possible.
ABSTRACT
The rhizosphere is a microhabitat where there is an intense chemical dialogue between plants and microorganisms. The two coexist and develop synergistic actions, which can promote plants' functions and productivity, but also their capacity to respond to stress conditions, including heavy metal (HM) contamination. If HMs are present in soils used for agriculture, there is a risk of metal uptake by edible plants with subsequent bioaccumulation in humans and animals and detrimental consequences for their health. Plant productivity can also be negatively affected. Many bacteria have defensive mechanisms for resisting heavy metals and, through various complex processes, can improve plant response to HM stress. Bacteria-plant synergic interactions in the rhizosphere, as a homeostatic ecosystem response to HM disturbance, are common in soil. However, this is hard to achieve in agroecosystems managed with traditional practices, because concentrating on maximizing crop yield does not make it possible to establish rhizosphere interactions. Improving knowledge of the complex interactions mediated by plant exudates and secondary metabolites can lead to nature-based solutions for plant health in HM contaminated soils. This paper reports the main ecotoxicological effects of HMs and the various compounds (including several secondary metabolites) produced by plant-microorganism holobionts for removing, immobilizing and containing toxic elements.
ABSTRACT
Anaerobic digestion is one of the best ways to re-use animal manure and agricultural residues, through the production of combustible biogas and digestate. However, the use of antibiotics for preventing and treating animal diseases and, consequently, their residual concentrations in manure, could introduce them into anaerobic digesters. If the digestate is applied as a soil fertilizer, antibiotic residues and/or their corresponding antibiotic resistance genes (ARGs) could reach soil ecosystems. This work investigated three common soil emerging contaminants, i.e., sulfamethoxazole (SMX), ciprofloxacin (CIP), enrofloxacin (ENR), their ARGs sul1, sul2, qnrS, qepA, aac-(6')-Ib-cr and the mobile genetic element intI1, for one year in a full scale anaerobic plant. Six samplings were performed in line with the 45-day hydraulic retention time (HRT) of the anaerobic plant, by collecting input and output samples. The overall results show both antibiotics and ARGs decreased during the anaerobic digestion process. In particular, SMX was degraded by up to 100%, ENR up to 84% and CIP up to 92%, depending on the sampling time. In a similar way, all ARGs declined significantly (up to 80%) in the digestate samples. This work shows how anaerobic digestion can be a promising practice for lowering antibiotic residues and ARGs in soil.
ABSTRACT
The anionic surfactant SLES (sodium lauryl ether sulfate) is an emerging contaminant, being the main component of foaming agents that are increasingly used by the tunnel construction industry. To fill the gap of knowledge about the potential SLES toxicity on plants, acute and chronic effects were assessed under controlled conditions. The acute ecotoxicological test was performed on Lepidum sativum L. (cress) and Zea mays L. (maize). Germination of both species was not affected by SLES in soil, even at concentrations (1200 mg kg-1) more than twice higher than the maximum realistic values found in contaminated debris, thus confirming the low acute SLES toxicity on terrestrial plants. The root elongation of the more sensitive species (cress) was instead reduced at the highest SLES concentration. In the chronic phytotoxicity experiment, photosynthesis of maize was downregulated, and the photosynthetic performance (PITOT) significantly reduced already under realistic exposures (360 mg kg-1), owing to the SLES ability to interfere with water and/or nutrients uptake by roots. However, such reduction was transient, likely due to the rapid biodegradation of the surfactant by the soil microbial community. Indeed, SLES amount decreased in soil more than 90% of the initial concentration in only 11 days. A significant reduction of the maximum photosynthetic capacity (Pnmax) was still evident at the end of the experiment, suggesting the persistence of negative SLES effects on plant growth and productivity. Overall results, although confirming the low phytotoxicity and high biodegradability of SLES in natural soils, highlight the importance of considering both acute and nonlethal stress effects to evaluate the environmental compatibility of soil containing SLES residues.
Subject(s)
Ether , Soil Pollutants , Germination , Photosynthesis , Plant Roots , Polyethylene Glycols , Sodium , Sodium Dodecyl Sulfate , Soil Pollutants/toxicity , Zea maysABSTRACT
Anaerobic digestion (AD) treatment of cattle manure and slurry makes it possible to produce biogas, a renewable and storable biofuel, as well as digestate, a residual organic matter that can be used to replace chemical fertilizers. On the other hand, the intense use of antibiotics (e.g., sulfamethoxazole) in animal husbandry practices is showing increasing negative impacts resulting from the release of still metabolically active molecules into agroecosystems. In the present study, cattle manure collected from an AD plant-feeding tank was used as feedstock for AD experiments in which some batches were spiked with 5 mg L-1 of sulfamethoxazole (SMX). Adding the antibiotic affected the microbial community dynamic; in particular, the efficiency of the acidogenic and acetogenic phases of the process corresponded to higher CH4 and H2 production than in the control. SMX was also degraded, and at the end of the experiment (69 days), just 20% of its initial concentration was found. The relative abundance (ARG/16S) of resistance genes sul1, sul2, and the proxy intI1 initially found in the ingestate decreased during the AD in both the spiked and control batches, suggesting that this process lowers the likelihood of antibiotic resistance genes spreading.
ABSTRACT
Due to their widespread use in industrial applications in recent decades, Polychlorobiphenyls (PCBs) and heavy metals (HMs) are the most common soil contaminants worldwide, posing a risk for both ecosystems and human health. In this study, a poplar-assisted bioremediation strategy has been applied for more than 4 years to a historically contaminated area (PCBs and HMs) in Southern Italy using the Monviso poplar clone. This clone was effective in promoting a decrease in all contaminants and an increase in soil quality in terms of organic carbon and microbial abundance. Moreover, a significant shift in the structure and predicted function of the belowground microbial community was also observed when analyzing both DNA and cDNA sequencing data. In fact, an increase in bacterial genera belonging to Proteobacteria able to degrade PCBs and resist HMs was observed. Moreover, the functional profiling of the microbial community predicted by PICRUSt2 made it possible to identify several genes associated with PCB transformation (e.g., bphAa, bphAb, bphB, bphC), response to HM oxidative stress (e.g., catalase, superoxide reductase, peroxidase) and HM uptake and expulsion (e.g., ABC transporters). This work demonstrated the effectiveness of the poplar clone Monviso in stimulating the natural belowground microbial community to remove contaminants and improve the overall soil quality. It is a practical example of a nature based solution involving synergic interactions between plants and the belowground microbial community.
ABSTRACT
The anionic surfactant Sodium Lauryl Ether Sulfate (SLES) is the principal component of several commercial foaming products for soil conditioning in the tunneling industry. Huge amounts of spoil material are produced during the excavation process and the presence of SLES can affect its re-use as a by-product. Anionic surfactants can be a risk for ecosystems if occurring in the environment at toxic concentrations. SLES biodegradability is a key issue if the excavated soil is to be reused. The aim of this study was to identify bacteria able to degrade SLES, so that it could potentially be used in bioaugmentation techniques. Enrichment cultures were performed using bacterial populations from spoil material collected in a tunnel construction site as the inoculum. A bacterial consortium able to grow in a few hours with SLES concentrations from 125 mg/L to 2 g/L was selected and then identified by Next Generation Sequencing analysis. Most of bacteria identified belonged to Gamma-Proteobacteria (99%) and Pseudomonas (ca 90%) was the predominant genus. The bacterial consortium was able to degrade 94% of an initial SLES concentration of 250 mg/L in 9 h. A predictive functional analysis using the PICRUSt2 software showed the presence of esterase enzymes, responsible for SLES degradation. The bacterial consortium selected could be useful for its possible seeding (bioaugmentation) on spoil material from tunneling excavation.
ABSTRACT
Rosmarinus officinalis is an evergreen aromatic plant with important commercial interest as it contains numerous essential oils (composed of terpenoid compounds) and phenolic constituents (natural antioxidant compounds). This work aims at evaluating the concomitant effects of different inorganic and organic fertilization treatments and the subsequent increases in soil nutrient availability on terpenoids and other carbon-based secondary metabolites, e.g., flavonoids and phenolic compounds, in Rosmarinus officinalis leaves. The results showed that, as expected, the structural carbohydrate content (lignocellulosic compounds) in stems was higher in fertilized plants than in controls. Additionally, positive correlations were observed of the absolute amounts of total terpenoids and some single terpenoid compounds with N or P contents in leaves. On the contrary, the phenolic and flavonoid concentrations in all the rosemary plant parts were lower with the fertilization treatments. Indeed, negative correlations between the phenolic compounds (and flavonoids) and N in rosemary leaves were also found. Overall, the results suggest that the terpenoid production's response to fertilization was due to N, which is essential for protein synthesis and terpene synthase activity, and to P, which is necessary for the synthesis of both terpenoid precursors and ATP and NADPH, also needed for terpenoid synthesis. On the other hand, the basis for the fertilization's effects on the production of phenolic compounds is the direct nitrogen trade-off between growth and the shikimic acid pathway by which phenolics compounds are synthesized.
ABSTRACT
Sodium lauryl ether sulphate (SLES) is the main chemical component in several lubricant products used for soil conditioning in the mechanized excavation industry using Earth Pressure Balance-Tunnel Boring Machines. During the tunnelling process, huge amounts of excavated soil are produced and the SLES presence can affect the subsequent re-use of this material as a by-product. Currently, there is still no regulatory indication of reliable and sensitive bioassays for monitoring soil quality during the excavation process. The main objective of this work was to verify if the Vibrio fischeri screening test was suitable as a consistent and precautionary tool for this specific purpose. Firstly, the ecotoxicity (EC20 and EC50) of the SLES standard solution and three commercial products (SLES content from 10 to 50%) were evaluated to select the most environmental friendly product. Subsequently, soil samples from about 2 years of tunnelling in a real construction site, conditioned with the selected product, were evaluated for their environmental compatibility with the prescriptions of an Italian site-specific protocol. The latter established 2 mg/L as a threshold value for SLES concentration in soil water extracts and a no toxic response (≤20%) for the Vibrio fischeri test. The comparison of the bacterium bioluminescence inhibition values (%) with analytical determinations showed an ecotoxicity when SLES was >2 mg/L. The toxicity was directly related to SLES concentration, indicating that the V. fischeri test and the SLES analyses are suitable tools for assessing excavated soil as a by-product, ensuring its safe reuse in accordance with a green production process (circular economy).