An optical photothermal infrared investigation of lymph nodal metastases of oral squamous cell carcinoma.

In this study, optical photothermal infrared (O-PTIR) spectroscopy combined with machine learning algorithms were used to evaluate 46 tissue cores of surgically resected cervical lymph nodes, some of which harboured oral squamous cell carcinoma nodal metastasis. The ratios obtained between O-PTIR chemical images at 1252 cm-1 and 1285 cm-1 were able to reveal morphological details from tissue samples that are comparable to the information achieved by a pathologist's interpretation of optical microscopy of haematoxylin and eosin (H&E) stained samples. Additionally, when used as input data for a hybrid convolutional neural network (CNN) and random forest (RF) analyses, these yielded sensitivities, specificities and precision of 98.6 ± 0.3%, 92 ± 4% and 94 ± 5%, respectively, and an area under receiver operator characteristic (AUC) of 94 ± 2%. Our findings show the potential of O-PTIR technology as a tool to study cancer on tissue samples.

Validation of field-based running tests to determine maximal aerobic speed in professional rugby league.

Practitioners place importance on high-speed running (HSR) to monitor training practice and match-play demands, whilst attempting to maximise fitness and minimize the risk of injury occurrence. Practitioners apply various methods to quantify HSR, such as absolute thresholds, percentage of maximum sprint speed and maximal aerobic speed (MAS). A recent survey demonstrates the 5-minute run and 1200m shuttle test (ST) to be implemented among rugby league practitioners to quantify HSR by incorporating MAS. However, it is unclear as to how valid these methods are to accurately quantify MAS. Therefore, the aim of this study was to assess the validity of the 5-minute run and 1200m ST when compared to a gold standard measure for MAS. Twenty 1st team professional rugby league players competing in the European Super League participated in this study. Players were required to complete an incremental treadmill test, 5-minute run and 1200m ST over a two-week period in pre-season. MAS, peak heart rate (HRmax), peak lactate (Lapeak) and rating of perceived exertion (RPE) where collected upon completion of each test. Results demonstrated the 1200m ST to have a higher correlation for MAS than the 5-minute run (1200m ST: r = 0.73, 5-minute run: r = 0.64). However, when assessing validity using the level of agreement between data, the 5-minute run underreported MAS by 0.45 m·s-1 whereas the 1200m ST underreported MAS by 0.77 m·s-1. Ultimately, both field-based tests used in this study underreport MAS when compared to an incremental treadmill test, although the 5-minute run provides a closer agreement and therefore a more valid measurement for MAS than the 1200m ST.

Talent identification in soccer: The influence of technical, physical and maturity-related characteristics on a national selection process.

The present study examines the influence of technical, physical, and relative age characteristics on players selection success within the Scottish Performance School trials. Ninety adolescent players (81 males, 9 females; mean ± standard deviation: age = 11.3 ± 0.4 years, height = 149.6 ± 6.9 cm, mass 38.1 ± 4.7 kg) performed a battery of physical fitness (20m Sprint, CMJ, 5-0-5 agility test), anthropometric, and 8 small-sided games (SSG; 9v9) as part of a talent identification (TID) programme. Players technical (ball touches, time on the ball, high-speed releases) and locomotor activities (high-speed running distance, sprint distance, accelerations, and decelerations) were monitored using foot-mounted inertial measurements units during SSG's. The data was analysed using independent sample T-tests. Mann-Whitney U analyses were conducted to examine the differences between groups whose data was determined as being (non)parametric, with Cohen effect sizes applied. Successful players performed significantly better during physical tests (Effect size ± confidence limits: Left 5-0-5 = -0.89±0.13, Right 5-0-5 = -0.51±0.11), had significantly higher locomotor activities during SSG (high-intensity distance = 0.4±26.6, horizontal accelerations = 0.59±1.19) and significantly higher technical outputs during SSG (touches = 0.71±6.1, releases = 0.49±2.5, high-speed releases = 0.59±2.7, time on the ball = 0.52±3.4) compared to unsuccessful players. Successful players had significantly higher locomotor activities and technical outputs during SSG than their unsuccessful counterparts. Monitoring technical and locomotor activities during SSG may compliment or replace physical testing batteries for assessing TID processes in soccer.

Prediction of prognosis in oral squamous cell carcinoma using infrared microspectroscopy.

BACKGROUND: Estimation of prognosis of oral squamous cell carcinoma (OSCC) is inaccurate prior to surgery, only being effected following subsequent pathological analysis of the primary tumour and excised lymph nodes. Consequently, a proportion of patients are overtreated, with an increase in morbidity, or undertreated, with inadequate margins and risk of recurrence. We hypothesise that it is possible to accurately characterise clinical outcomes from infrared spectra arising from diagnostic biopsies. In this first step, we correlate survival with IR spectra derived from the primary tumour. METHODS: Infrared spectra were collected from tumour tissue from 29 patients with OSCC and subject to classification modelling. RESULTS: The model had a median AUROC of 0.89 with regard to prognosis, a median specificity of 0.83, and a hazard ratio of 6.29 in univariate Cox proportional hazard modelling. CONCLUSION: The data suggest that FTIR spectra may be a useful early biomarker of prognosis in OSCC.

Professional soccer practitioners' perceptions of using performance analysis technology to monitor technical and tactical player characteristics within an academy environment: A category 1 club case study.

This study aimed to identify professional soccer practitioners' perceptions of the application of performance analysis technology within a single academy club. Secondary aims were to understand the importance that practitioners place on monitoring technical and tactical player characteristics, current practices, and barriers to implementing wearable technology. Utilising a mixed method design, forty-four professional soccer academy practitioners (Age = 32 ± 5.8; Years of experience = 8.5 ± 6.2) completed an online survey intended to examine present trends, professional practices, and perceptions regarding the monitoring of technical and tactical metrics. Frequency and percentages of responses for individual items were calculated. Subsequently, eleven participants who were directly involved with the monitoring of players were recruited to participate in a semi-structured interview. Interview data was transcribed and analysed using a combination of deductive and inductive approaches to identify key themes. The main findings across both phases of the study were that (1) technical and tactical metrics are monitored more frequently in matches (Technical: 89%; tactical: 91%) than training (Technical: 80%; Tactical 64%), predominantly due to time constraints and staffing numbers. Accordingly, practitioners believe that it would be beneficial to have an automated way of tracking technical (79%) and tactical (71%) metrics and would consider using a foot-mounted IMU to do so (technical (68%) and tactical (57%)). (2) Monitoring technical and tactical metrics is beneficial to assist with player development and to enrich feedback provision (3) Key stake holders, coaches and players should be informed of the relevance and rationale for monitoring. (4) For successful implementation and continued uptake, the information delivered needs to be both meaningful and easy to understand. Findings suggest that although participants appreciate the need to collect technical and tactical metrics, they are keen to ensure that wearable-derived data does not replace experiential and contextual knowledge. Accordingly, practitioners need to work closely with coaches to determine the contexts in which metrics may or may not prove useful. However, as the sample comprised of participants from a single academy, further studies including more practitioners are warranted. Likewise, future research could also extend to include academy soccer players perceptions too.

A Methodological Comparison of Protocols and Analytical Techniques to Assess the Outcome Measures of Submaximal Fitness Tests.

BACKGROUND: Submaximal fitness test (SMFT) outcome measures are frequently collected with a wide array of technologies and methodological approaches. PURPOSE: To examine the test-retest reliability of various SMFT outcome measures derived from different protocols and analytical techniques. METHODS: Twenty-six semiprofessional adult soccer players performed 3 SMFT protocols, including 2 continuous (3 min, 11 and 12.8 km·h-1) and 1 intermittent (4 × 50 m, 18 km·h-1) twice, each separated by 7 days. Heart-rate (HR) indices (exercise HR, HR recovery) and scapula-mounted (PlayerLoad vector magnitude) and foot-mounted (flight time and contact time, stride length) microelectrical mechanical system-derived variables were collected using different time frames and analytical approaches adopted in the literature and practice. Absolute reliability was quantified as the group mean difference, typical error of measurement, also expressed as the coefficient of variation (where appropriate) and standardized units (ie, d). Intraclass correlation coefficient was used to quantify relative reliability. RESULTS: The highest degrees of reliability were evident for exercise HR (typical error: 1.0%-1.6% points), the vertical component of PlayerLoad (expressed in arbitrary units; coefficient of variation: 5.5%-7.0%), and contact time (coefficient of variation: 1.5%-3.0%). These estimates were not influenced by SMFT protocol or analytical approach. All other measures displayed poorer reliability and/or were different between protocols and analytical methods. CONCLUSIONS: SMFT protocols impact the test-retest reliability of various outcome measures; however, exercise HR, vertical PlayerLoad, and contact time (derived from foot-mounted micro-electrical mechanical systems) appear to have stable measurement properties to assist the assessment of aerobic capacity and lower-limb neuromuscular status, respectively.

Tissue discrimination in head and neck cancer using image fusion of IR and optical microscopy.

A regression-based fusion algorithm has been used to merge hyperspectral Fourier transform infrared (FTIR) data with an H&E image of oral squamous cell carcinoma metastases in cervical lymphoid nodal tissue. This provides insight into the success of the ratio of FTIR absorbances at 1252 cm-1 and 1285 cm-1 in discriminating between these tissue types. The success is due to absorbances at these two wavenumbers being dominated by contributions from DNA and collagen, respectively. A pixel-by-pixel fit of the fused spectra to the FTIR spectra of collagen, DNA and cytokeratin reveals the contributions of these molecules to the tissue at high spatial resolution.

Concurrent validity and between-unit reliability of a foot-mounted inertial measurement unit to measure velocity during team sport activity.

The concurrent validity and between-unit reliability of a foot-mounted inertial measurement unit (F-IMU) was investigated during linear and change of direction running drills. Sixteen individuals performed four repetitions of two drills (maximal acceleration and flying 10 m sprint) and five repetitions of a multi-directional movement protocol. Participants wore two F-IMUs (Playermaker) and 10 retro-reflective markers to allow for comparisons to the criterion system (Qualisys). Validity of the F-IMU derived velocity was assessed via root-mean-square error (RMSE), 95% limits of agreement (LoA) and mean difference with 95% confidence interval (CI). Between-unit reliability was assessed via intraclass correlation (ICC) with 90% CI and 95% LoA. The mean difference for instantaneous velocity for all participants and drills combined was -0.048 ± 0.581 m ∙ s-1, the LoA were from -1.09 to -1.186 m ∙ s-1 and RMSE was 0.583 m ∙ s-1. The ICC ranged from 0.84 to 1, with LoA from -7.412 to 2.924 m ∙ s-1. Differences were dependent on the reference speed, with the greatest absolute difference (-0.66 m ∙ s-1) found at velocities above 7 m ∙ s-1. Between-unit reliability of the F-IMU ranges from good to excellent for all locomotor characteristics. Playermaker has good agreement with 3D motion capture for velocity and good to excellent between-unit reliability.

A Worldwide Survey on the Practices and Perceptions of Submaximal Fitness Tests in Team Sports.

PURPOSE: To survey team-sport practitioners on current practices and perceptions of submaximal fitness tests (SMFTs). METHODS: A convenience sample of team-sport practitioners completed an online survey (September to November 2021). Descriptive statistics were used to obtain information of frequencies. A mixed-model quantile (median) regression was employed to assess the differences between the perceived influence of extraneous factors. RESULTS: A total of 66 practitioners (74 discrete protocols) from 24 countries completed the survey. Time-efficient and nonexhaustive nature were considered the most important features of implementation. Practitioners prescribed a range of SMFTs, administered mostly on a monthly or weekly basis, but scheduling strategies appeared to differ across SMFT categories. Cardiorespiratory/metabolic outcome measures were collected in most protocols (n = 61; 82%), with the majority monitoring heart-rate-derived indices. Subjective outcome measures (n = 33; 45%) were monitored exclusively using ratings of perceived exertion. Mechanical outcome measures (n = 19; 26%) included either a combination of locomotor outputs (eg, distance covered) or variables derived from microelectrical mechanical systems. The perceived influence of extraneous factors on measurement accuracy varied according to outcome measure, and there was a lack of consensus among practitioners. CONCLUSIONS: Our survey showcases the methodological frameworks, practices, and challenges of SMFTs in team sports. The most important features for implementation perhaps support the use of SMFTs as a feasible and sustainable tool for monitoring in team sports. The wide variety of protocols, scheduling strategies, and outcome measures, along with their associated collection and analytical techniques, may reflect the absence of robust evidence regarding the application of SMFTs in team sports.

Metric-based analysis of FTIR data to discriminate tissue types in oral cancer.

A machine learning algorithm (MLA) has predicted the prognosis of oral potentially malignant lesions and discriminated between lymph node tissue and metastatic oral squamous cell carcinoma (OSCC). The MLA analyses metrics, which are ratios of Fourier transform infrared absorbances, and identifies key wavenumbers that can be associated with molecular biomarkers. The wider efficacy of the MLA is now shown in the more complex primary OSCC tumour setting, where it is able to identify seven types of tissue. Three epithelial and four non-epithelial tissue types were discriminated from each other with sensitivities between 82% and 96% and specificities between 90% and 99%. The wavenumbers involved in the five best discriminating metrics for each tissue type were tightly grouped, indicating that small changes in the spectral profiles of the different tissue types are important. The number of samples used in this study was small, but the information will provide a basis for further, larger investigations.

Submaximal Fitness Test in Team Sports: A Systematic Review and Meta-Analysis of Exercise Heart Rate Measurement Properties.

BACKGROUND: Submaximal fitness tests (SMFT) are a pragmatic approach for evaluating athlete's physiological state, due to their time-efficient nature, low physiological burden and relative ease of administration in team sports settings. While a variety of outcome measures can be collected during SMFT, exercise heart rate (HRex) is the most popular. Understanding the measurement properties of HRex can support the interpretation of data and assist in decision making regarding athlete's current physiological state and training effects. OBJECTIVES: The aims of our systematic review and meta-analysis were to: (1) establish meta-analytic estimates of SMFT HRex reliability and convergent validity and (2) examine the moderating influence of athlete and protocol characteristics on the magnitude of these measurement properties. METHODS: We conducted a systematic literature search with MEDLINE, Scopus and Web of Science databases for studies published up until January 2022 since records began. Studies were considered for inclusion when they  included team sports athletes and the reliability and/or convergent validity of SMFT HRex was investigated. Reliability statistics included the group mean difference (MD), typical error of measurement (TE) and intraclass correlation coefficient (ICC) derived from test-retest(s) designs. Pearson's correlation coefficient (r) describing the relationship between SMFT HRex and a criterion measure of endurance performance was used as the statistic for convergent validity. Qualitative assessment was conducted using risk of bias assessment tool for non-randomised studies. Mixed-effects, multilevel hierarchical models combined with robust variance estimate tests were performed to obtain pooled measurement property estimates, effect heterogeneity, and meta-regression of modifying effects. RESULTS: The electronic search yielded 21 reliability (29 samples) and 20 convergent validity (29 samples) studies that met the inclusion criteria. Reliability meta-analysis indicated good absolute (MD = 0.5 [95% CI 0.1 to 0.9] and TE = 1.6 [95% CI 1.4 to 1.9] % points), and high relative (ICC = 0.88 [95% CI 0.84 to 0.91]) reliability. Convergent validity meta-analysis indicated an inverse, large relationship (r = - 0.58 [95% CI - 0.62 to - 0.54]) between SMFT HRex and endurance tests performance. Meta-regression analyses suggested no meaningful influence of SMFT protocol or athlete characteristics on reliability or convergent validity estimates. CONCLUSIONS: Submaximal fitness test HRex is a reliable and valid proxy indicator of endurance performance in team sport athletes. Athlete and SMFT protocol characteristics do not appear to have a meaningful effect on these measurement properties. Practitioners may implement SMFT HRex for monitoring athlete's physiological state by using our applied implications to guide the interpretation of data in practice. Future research should examine the utility of SMFT HRex to track within-athlete changes in aerobic capacity, as well as any further possible effects of SMFT protocols design elements or HRex analytical methods on measurement properties. Registration Protocol registration can be found in Open Science Framework and available through https://doi.org/10.17605/OSF.IO/9C2JV .

Does biologically categorised training alter the perceived exertion and neuromuscular movement profile of academy soccer players compared to traditional age-group categorisation?

The individual response to load is multifactorial and complicated by transient temporal changes in biological maturation. The period surrounding peak height velocity exposes potentially "fragile" individuals to systematic, age-related increases in training loads. Bio-banding allows practitioners to manage the biological diversity and align training to the individual development needs . This study explores the acute impact of maturation on neuromuscular performance and perceived intensity through comparing both chronological and bio-banded training sessions. 55 male soccer players (mean ± SD; age 13.8 ± 1.4 years) were recruited from an EPPP academy. Following a warm-up and standardised sub-maximal run (30-15IFT), players competed in five bouts of 5-min 6v6 small-sided games (SSGs) before repeating the standardised sub-maximal run. The sessions were repeated on three occasions with chronological SSGs and the same with bio-banded SSGs wearing foot-mounted inertial measurement units (PlayerMakerTM) with differential ratings of perceived exertion used to quantify internal loads. Mixed linear modelling indicated maturity-specific pre-post differences in neuromuscular response, stride length and cadence having contrasting responses pre- (reduced) and post-PHV (increased), and larger changes in post sessions stiffness for pre- (∼18.6 kN·m-1) and circa-PHV (∼12.1 kN·m-1) players. Secondly, there were small to large differences in neuromuscular response (RSI, stride length, stiffness, and contact time) and perceptions of intensity between conditions, with bio-banding generally reducing pre-post changes. Bio-banding may therefore offer a mechanism to prescribe maturity-specific training loads which may help to alleviate the impact of repeated exposure to high-intensity activity, thus reducing injury risk whilst promoting long-term player development.Highlights Utilising a sub-maximal running protocol (30-15IFT) with foot mounted accelerometers can detect maturity specific responses to football specific training activity, which aligns with subjective perceptions of intensity.Chronologically derived small-sided games elicit different acute responses between players of varying maturity status, which is somewhat negated when bio-banded small-sided games are used instead.Bio-banding training sessions may offer practitioners a practical way of managing maturity-specific trainings load to reduce injury risk and promote long-term players development.

Locomotor and technical characteristics of female soccer players training: exploration of differences between competition standards.

OBJECTIVES: To (i) quantify the differences in locomotor and technical characteristics between different drill categories in female soccer and (ii) explore the training drill distributions between different standards of competition. METHODS: Technical (ball touches, ball releases, high-speed ball releases) and locomotor data (total distance, high-speed running distance [>5.29 m∙s-1]) were collected using foot-mounted inertial measurement units from 458 female soccer players from three Women's Super League (WSL; n = 76 players), eight Women's Championship (WC; n = 217) and eight WSL Academy (WSLA; n = 165) teams over a 28-week period. Data were analysed using general linear mixed effects. RESULTS: Across all standards, the largest proportion of time was spent in technical (TEC) (WSL = 38%, WC = 28%, WSLA = 29%) and small-sided extensive games (SSGe) (WSL = 20%, WC = 31%, WSLA = 30%) drills. WSL completed more TEC and tactical (TAC) training whilst WC and WSLA players completed more SSGe and possession (POS) drills. Technical drills elicited the highest number of touches, releases and the highest total distance and high-speed activity. Position-specific drills elicited the lowest number of touches and releases and the lowest total distance. When the technical and locomotor demand of each drill were made relative to time, there were limited differences between drills, suggesting drill duration was the main moderating factor. CONCLUSION: Findings provide novel understanding of the technical and locomotor demands of different drill categories in female soccer. These results can be used by coaches and practitioners to inform training session design.

Prediction of malignant transformation in oral epithelial dysplasia using machine learning.

A machine learning algorithm (MLA) has been applied to a Fourier transform infrared spectroscopy (FTIR) dataset previously analysed with a principal component analysis (PCA) linear discriminant analysis (LDA) model. This comparison has confirmed the robustness of FTIR as a prognostic tool for oral epithelial dysplasia (OED). The MLA is able to predict malignancy with a sensitivity of 84 ± 3% and a specificity of 79 ± 3%. It provides key wavenumbers that will be important for the development of devices that can be used for improved prognosis of OED.

A multi-club analysis of the locomotor training characteristics of elite female soccer players.

OBJECTIVE: Quantifying differences in locomotor characteristics of training between two competition levels and between training days within elite female soccer players. METHODS: Foot-mounted inertial measurement unit (Playermaker) data were collected from 293 players from three Women's Super League (WSL; n = 76) and eight Women's Championship (WC; n = 217) teams over a 28-week period. Data were analysed using partial least squares correlation analysis to identify relative variable importance and linear mixed effects models to identify magnitude of effects. RESULTS: WSL players performed more high-speed running distance (HSR; >5.29 m∙s-1), sprint distance (SpD; >6.26 m∙s-1), acceleration (ACC; >3 m∙s-2) and deceleration (DEC; <-3 m∙s-2) distance than WC players. The largest difference between WSL and WC in HSR and HSR per minute occurred on MD-4, (354.7 vs. 190.29 m and 2.8 vs. 1.7 m∙min-1). On MD-2, WSL players also covered greater SpD (44.66 vs. 12.42 m), SpD per minute (0.38 vs. 0.11 m∙min-1) and HSR per minute (1.67 vs. 0.93 m∙min-1). Between training days both WSL and WC teams reduced HSR and SpD but not ACC and DEC distance from MD-4 to MD-2, with MD-4 the highest training day of the week. CONCLUSION: MD-4 is a key training day discriminating between competitive level. HSR and SpD volume and intensity is tapered in WSL and WC players, however there is less clear taper of ACC or DEC. As such, WC teams could increase the volume and intensity of HSR on MD-4 to mimic locomotor activities of those at a higher standard.

Submaximal Fitness Tests in Team Sports: A Theoretical Framework for Evaluating Physiological State.

Team-sports staff often administer non-exhaustive exercise assessments with a view to evaluating physiological state, to inform decision making on athlete management (e.g., future training or recovery). Submaximal fitness tests have become prominent in team-sports settings for observing responses to a standardized physical stimulus, likely because of their time-efficient nature, relative ease of administration, and physiological rationale. It is evident, however, that many variations of submaximal fitness test characteristics, response measures, and monitoring purposes exist. The aim of this scoping review is to provide a theoretical framework of submaximal fitness tests and a detailed summary of their use as proxy indicators of training effects in team sports. Using a review of the literature stemming from a systematic search strategy, we identified five distinct submaximal fitness test protocols characterized in their combinations of exercise regimen (continuous or intermittent) and the progression of exercise intensity (fixed, incremental, or variable). Heart rate-derived indices were the most studied outcome measures in submaximal fitness tests and included exercise (exercise heart rate) and recovery (heart rate recovery and vagal-related heart rate variability) responses. Despite the disparity between studies, these measures appear more relevant to detect positive chronic endurance-oriented training effects, whereas their role in detecting negative transient effects associated with variations in autonomic nervous system function is not yet clear. Subjective outcome measures such as ratings of perceived exertion were less common in team sports, but their potential utility when collected alongside objective measures (e.g., exercise heart rate) has been advocated. Mechanical outcome measures either included global positioning system-derived locomotor outputs such as distance covered, primarily during standardized training drills (e.g., small-sided games) to monitor exercise performance, or responses derived from inertial measurement units to make inferences about lower limb neuromuscular function. Whilst there is an emerging interest regarding the utility of these mechanical measures, their measurement properties and underpinning mechanisms are yet to be fully established. Here, we provide a deeper synthesis of the available literature, culminating with evidence-based practical recommendations and directions for future research.

The effect of bio-banding on technical and tactical indicators of talent identification in academy soccer players.

AIM: The aim of this study was to examine the effect of bio-banding on technical and tactical markers of talent identification in 11- to 14-year-old academy soccer players. METHODS: Using a repeated measures design, 92 players were bio-banded using percentage of estimated adult stature attainment (week 1), maturity-offset (week 2) and a mixed-maturity method (week 3). All players contested five maturity (mis)matched small-sided games with technical and tactical variables measured. Data were analysed using a series of Bayesian hierarchical models, fitted with different response distributions and different random and fixed effect structures. RESULTS: Despite differences during maturity-matched bio-banding for post-peak height velocity players, very few tactical differences were evident during the remaining maturity-matched and mis-matched fixtures for both banding methods. In fact, the results showed no consistent differences across both banding methods for practitioner and video analysis-derived technical performance characteristics during maturity matched and mis-matched fixtures. Both bio-banding methods explained similar levels of variance across the measured variables. CONCLUSION: Maturity-matched bio-banding had some effect on both technical and tactical characteristics of players during maturity-matched bio-banded formats. That said, this trend remained during maturity mis-matched bio-banded formats which restricts the conclusions that can be made regarding the effectiveness of bio-banding to manipulate technical and tactical measures in academy soccer players.

Examination of differential ratings of perceived exertion (dRPE) during bio-banded small-sided games.

The aims of the current study were to investigate the use of dRPE with academy soccer players to: 1) examine the effect of bio-banded and non-bio-banded maturity groups within SSG on players dRPE; 2) describe the multivariate relationships between dRPE measures investigating the sources of intra and inter-individual variation, and the effects of maturation and bio-banding. Using 32 highly trained under (U) 12 to U14 soccer players (mean (SD) age 12.9 (0.9) years, body mass 46.4 (8.5) kg and stature 158.2 (14.9) cm) academy soccer players from two English professional male soccer academies. Players were categorised according to somatic maturity status using estimated percentage of adult stature attainment, with players randomly assigned into teams to play 4v4 SSG. The study used a repeated measures design, whereby the selected players participated within 6 bio-banded (maturity matched [pre-PHV Vs pre-PHV and post-PHV vs post PHV] and miss-matched [pre-PHV vs post-PHV] and 6 mixed maturity SSG at their respective clubs. Using mixed and fixed effect regression models, it was established hat pre-PHV players exhibited higher dRPE compared with their post-PHV counterparts. Mixed bio-banded games reported higher dRPE outputs overall. Variation in dRPE measures across a series of bio-banded games are caused by both between and within sources of variation in relatively equal amounts. Across a series of bio-banded games, the four dRPE measures do not provide unique information, and between variation is best expressed by one or two highly correlated components, with within variation best explained by a single equally loaded component. Using a bio-banding SSG design study, we have shown that pre-PHV players report higher subjective measures of exertion than post-PHV players during. Additionally, when evenly mixing players based on measures of maturation, higher measures of perceived exertion were generally reported.

Quantifying technical actions in professional soccer using foot-mounted inertial measurement units.

OBJECTIVES: This study aimed to (i) establish the concurrent validity and intra-unit reliability of a foot-mounted inertial measurement unit for monitoring soccer technical actions, (ii) quantify the within-microcycle inter-positional differences in the technical actions of professional soccer training, and (iii) determine the influence of drill category on the technical actions of professional soccer training. METHODS: Twenty-one professional soccer players' technical performance data (ball touches, releases, ball touches per minute, releases per minute), collected during training sessions throughout twenty-four weekly microcycles, were analysed using general linear modelling. RESULTS: The inertial measurement unit exhibited good concurrent validity (PA = 95.1% - 100.0%) and intra-unit reliability (PA = 95.9% - 96.9%, CV = 1.4% - 2.9%) when compared with retrospective video analyses. The most ball touches (X‾ = 218.0) and releases (X‾ = 110.8) were observed on MD - 1, with MD - 5 eliciting the highest frequency of ball touches (X‾ = 3.8) and releases (X‾ = 1.7) per minute. Central midfielders performed the most ball touches (X‾ = 221.9), releases (X‾ = 108.3), ball touches per minute (X‾ = 3.4) and releases per minute (X‾ = 1.6). Small-sided games evoked more ball touches (X‾diff = 1.5) and releases per minute (X‾diff = 0.1) than previously reported in match-play. The fewest ball touches (X‾ = 1.2) and releases per minute (X‾ = 0.5) were observed during tactical drills. CONCLUSION: The results of this study provide a novel understanding of the within-microcycle, inter-positional and drill category differences in the technical actions performed by professional players during training.

Prediction of malignant transformation in oral epithelial dysplasia using infrared absorbance spectra.

Oral epithelial dysplasia (OED) is a histopathologically-defined, potentially premalignant condition of the oral cavity. The rate of transformation to frank carcinoma is relatively low (12% within 2 years) and prediction based on histopathological grade is unreliable, leading to both over- and under-treatment. Alternative approaches include infrared (IR) spectroscopy, which is able to classify cancerous and non-cancerous tissue in a number of cancers, including oral. The aim of this study was to explore the capability of FTIR (Fourier-transform IR) microscopy and machine learning as a means of predicting malignant transformation of OED. Supervised, retrospective analysis of longitudinally-collected OED biopsy samples from 17 patients with high risk OED lesions: 10 lesions transformed and 7 did not over a follow-up period of more than 3 years. FTIR spectra were collected from routine, unstained histopathological sections and machine learning used to predict malignant transformation, irrespective of OED classification. PCA-LDA (principal component analysis followed by linear discriminant analysis) provided evidence that the subsequent transforming status of these 17 lesions could be predicted from FTIR data with a sensitivity of 79 ± 5% and a specificity of 76 ± 5%. Six key wavenumbers were identified as most important in this classification. Although this pilot study used a small cohort, the strict inclusion criteria and classification based on known outcome, rather than OED grade, make this a novel study in the field of FTIR in oral cancer and support the clinical potential of this technology in the surveillance of OED.