Modulation of immune cum inflammatory pathway by earthworm granulation tissue extract in wound healing of diabetic rabbit model.

Regeneration is a rare occurrence in the animal kingdom, but the earthworm stands out as a remarkable example of this phenomenon. Recent research has highlighted the promising wound healing properties of extracts derived from earthworms. Therefore, we propose that earthworm granulation tissue extract (EGTE) may facilitate wound healing by regulating immune responses in a rabbit diabetic wound model. Electron microscopy reveals that 70 % EGTE possesses noteworthy porosity with spherical to irregularly oval configuration. Gas chromatography-mass spectrometry (GC-MS) Characterization of EGTE revealed higher levels of ergosta-5,7,22-trien-3-ol, (3. beta.,22E). In-Vitro studies revealed significant anti-oxidant, anti-inflammatory and anti-bacterial properties in dose dependent manner. Likewise, cytotoxicity assessments reveal that 70 % EGTE exhibits minimal harm to cells while displaying substantial antioxidant and anti-inflammatory activities. For In-Vivo studies excision wounds were created on the dorsal regions of the experimental animals and were divided as Group I (50 % EGTE), Group II (70 % EGTE), Group III (vehicle) and Group IV (distilled water). Over a 21-day observation period 70 % EGTE facilitated the early healing of wounds in the experimental animals, evident through prompt wound closure, granulation tissue formation, increased DNA content, enhanced tensile strength of the wound area and enhanced the expression/synthesis of wound healing markers/proteins. From these results it can be postulated that EGTE accelerates wound healing by immune modulation, dampening of inflammatory pathway and enhanced expression of growth markers. Henceforth making it promising candidate for therapeutic use in diabetic wound healing.

Does China Pakistan Economic Corridor become an avenue to achieve sustainable development goal no. 2 (food security) in Pakistan: Under the condition of COVID-19?

The post Covid-19 era steer towards stakeholder thinking, which demands to look for sustainable solutions in terms of other epidemics and food security is one of the most notable for the developing world. In South Asia, China Pakistan Economic Corridor could be an avenue for multi-layered socio-economic assistance where Pakistan can seek solution for one epidemic (food security) while engaged with the effects of pandemic through studying all the stakeholders. This could be a roadmap for the west-east or developed-underdeveloped countries reliance on one another for food security through economic corridor alike settings. Academically, it has been projected by the plethora of research that holistically and inclusively, stakeholder analysis is lacking in the available reservoir of research. Methodologically, the stakeholder analysis was performed in three layers-identification, investigation through social networks, and communication by prioritization matrix. The findings suggested that apart from stakeholders' differences in regard to interest, influence and knowledge, all of them agreed with the argument that China Pakistan Economic Corridor has enviably and irrefutably positive effects on food security. The policy implications project the need for integrated reports of various ministries to be unidirectional for having synergical effect and taking all the stakeholders into consideration as leaving any group behind will repeat the past results.

Sound propagation over soft ground without and with crops and potential for surface transport noise attenuation.

Growing demand on transportation, road, and railway networks has resulted in increased levels of annoyance from road traffic. Optimized use of green surfaces in combination with vegetation may be desirable as a method for reducing the noise impact of road traffic in urban and rural environments. Sound propagation over soft ground and through crops has been studied through outdoor measurements at short and medium ranges and through predictions. At lower frequencies, ground effect is dominant, and there is little or no attenuation due to crops. At higher frequencies above 3-4 kHz, the attenuation in crops is dominant. It was also found that the ground effects and the influence of crops can be treated independently and can be added to obtain the total effect. Sound attenuation by crops is the result of multiple scattering between the stems and leaves, loss of coherence, and viscous and thermal losses due to foliage. The major contribution is associated with viscous and thermal losses. A model for sound attenuation by vegetation is proposed. Insertion losses for a typical road traffic noise source have been calculated that result either by replacing hard ground with different types of acoustically soft ground or by growing crops along the road sides.

Diffraction assisted rough ground effect: models and data.

The destructive interferences observed in Excess Attenuation (EA) spectra over periodically and randomly spaced roughness elements with different cross-sectional profiles (semicylindrical, rectangular and wedge-shaped strips) have been investigated. If the roughness is spaced periodically, then two or three destructive interference maxima are observed in the same frequency range as the one or two observed with randomly distributed roughness. Roughness-induced surface waves are investigated also. Their amplitudes and the frequencies at which they occur are found to depend on the roughness height, mean center-to-center spacing and the extent to which the roughness is periodic. A semianalytical Multiple Scattering Theory and a numerical method (the Boundary Element Method) have been used to make predictions of the EA spectra which are compared with measurements. In addition it is found that the effective surface impedance spectra deduced from complex EA measurements over rough surfaces exhibit resonances similar to those observed for a hard-backed porous layer. On this basis a heuristic effective impedance model for rough hard surfaces is developed and the corresponding predictions of EA spectra are compared with data.

Surface waves over periodically-spaced rectangular strips.

Frequency- and time-domain measurements have been made on surfaces composed from parallel periodically-spaced rectangular strips (width: 0.0126 m, height: 0.0253 m) on an acoustically hard surface. The edge-to-edge spacing between the strips has been varied between 0.003 and 0.06 m. Frequency domain predictions show that when the spacing is small, these surfaces may be regarded as locally reacting rigid-framed hard-backed slit-pore layers with an effective depth slightly larger than the strip height, but when the spacing is comparable to the strip height or greater, the surfaces behave as periodically rough surfaces. Both frequency- and time-domain results show that surface waves of comparable magnitudes are created over the range of strip spacings studied but the frequency content of the acoustically induced surface waves decreases as the mean spacing is increased. It is found that surface wave dispersion is better predicted by the deduced effective impedance spectrum than by the slit-pore layer impedance model.

Aperiodicity effects on sound transmission through arrays of identical cylinders perpendicular to the ground.

Results of laboratory measurements of sound transmission through 5 × 10 arrays of meter long polyvinyl chloride pipes with lattice constants of 5 and 10 cm with filling fractions of 13% and 50% located either on medium density fibreboard or a layer of felt are reported. Ground effects and sonic crystal effects are found to be additive. Measurements and predictions show that, while there is little broadband advantage in a periodic configuration compared with a random one, a quasi-periodic arrangement in which the perturbation has a standard deviation equal to the scatterer diameter gives the best overall attenuation.

Outdoor ground impedance models.

Many models for the acoustical properties of rigid-porous media require knowledge of parameter values that are not available for outdoor ground surfaces. The relationship used between tortuosity and porosity for stacked spheres results in five characteristic impedance models that require not more than two adjustable parameters. These models and hard-backed-layer versions are considered further through numerical fitting of 42 short range level difference spectra measured over various ground surfaces. For all but eight sites, slit-pore, phenomenological and variable porosity models yield lower fitting errors than those given by the widely used one-parameter semi-empirical model. Data for 12 of 26 grassland sites and for three beech wood sites are fitted better by hard-backed-layer models. Parameter values obtained by fitting slit-pore and phenomenological models to data for relatively low flow resistivity grounds, such as forest floors, porous asphalt, and gravel, are consistent with values that have been obtained non-acoustically. Three impedance models yield reasonable fits to a narrow band excess attenuation spectrum measured at short range over railway ballast but, if extended reaction is taken into account, the hard-backed-layer version of the slit-pore model gives the most reasonable parameter values.