Assessing Activation Quality through Evaporative Drying Patterns of Zr-MOF (UiO-66) Colloidal Droplets.

We demonstrate a simple droplet diagnostic approach to monitor the UiO-66 MOF (metal-organic framework) synthesis and its quality using the sessile droplet drying phenomenon. Drying a sessile droplet involves evaporation-driven hydrodynamic flow and particle-nature-dependent self-assembled deposition. In general, the MOF synthesis process involves different sizes and physicochemical nature of particles in every synthesis stage. Equivalent quantities of each of purified pore-activated UiO-66 MOF, yet-to-be-purified pore-inactivated UiO-66 MOF, and reaction precursors of UiO-66 MOF give different deposition patterns when a well-dispersed aqueous droplet of these materials undergoes drying over substrates of varying stiffness and wettability. Yet-to-be-purified, pore-inactivated UiO-66 MOF nanoparticles undergo transport toward the droplet periphery, leading to a thick ring-like deposition at the dried droplet edge. Under appropriate drying conditions, such a deposit leads to desiccation-type mud-like reticular cracking. We study the origin of such ring-like deposits and cracks to understand how the surface charge density of UiO-66 particles controls their stability. We demonstrate that ZrOCl2 salt trapped in a nonpurified pore-inactivated UiO-66 MOF moiety is the principal reason for ring-like deposit formation and subsequent cracking in its dried aqueous droplet edge. Qualitatively, we identified Lewis acid salts that are capable of acting as BroÌ·nsted acid upon hydrolysis (like FeCl3, SnCl2, and ZrOCl2), influence surface charge density and colloidal stability of dispersed UiO-66 MOF particles. As a result, immediate particle coagulation is avoided, so those travel to the droplet edge, forming ring-like deposition and subsequent cracking upon drying. Further, we show that crack patterns on such deposits are highly dependent on the stiffness and temperature of depositing substrates via a competition between axial and lateral strains at the deposit-substrate interface.

Size and temperature drive nutrient retention potential across water bodies in China.

Lentic water bodies, including lakes, reservoirs, and wetlands, retain excess nutrients in runoff from agricultural and urban activities, and protect downstream water bodies from eutrophication. To develop effective nutrient mitigation strategies, it is important to understand the controls on nutrient retention in lentic systems and what drives variability between different systems and geographical regions. Efforts to synthesize water body nutrient retention at the global scale are biased toward studies from North America and Europe. Numerous studies published in Chinese Language journals exist in the extensive China National Knowledge Infrastructure (CNKI), but are missing from global synthesis due to their absence in English language journal databases. We address this gap by synthesizing data from 417 waterbodies in China to assess hydrologic and biogeochemical drivers of nutrient retention. In this study, we found median retention of 46 and 51% for nitrogen and phosphorus, respectively, across all water bodies in our national synthesis, and on average, wetlands retain more nutrients than lakes or reservoirs. The analysis of this dataset highlights the influence of water body size on first-order nutrient removal rate constants, as well as how regional temperature variations affect nutrient retention in water bodies. The dataset was used to calibrate the HydroBio-k model, which explicitly considers the effect of residence times and temperature on nutrient retention. Application of the HydroBio-k model across China reveals patterns of nutrient removal potential, where regions with a higher density of small water bodies retain more nutrients than others, such that regions like the Yangtze River Basin with a greater proportion of smaller water bodies have greater retention rates. Our results emphasize the importance of lentic systems and their function in nutrient removal and water quality improvement, as well as the drivers and variability of these functions at the landscape scale.

Advances in Catchment Science, Hydrochemistry, and Aquatic Ecology Enabled by High-Frequency Water Quality Measurements.

High-frequency water quality measurements in streams and rivers have expanded in scope and sophistication during the last two decades. Existing technology allows in situ automated measurements of water quality constituents, including both solutes and particulates, at unprecedented frequencies from seconds to subdaily sampling intervals. This detailed chemical information can be combined with measurements of hydrological and biogeochemical processes, bringing new insights into the sources, transport pathways, and transformation processes of solutes and particulates in complex catchments and along the aquatic continuum. Here, we summarize established and emerging high-frequency water quality technologies, outline key high-frequency hydrochemical data sets, and review scientific advances in key focus areas enabled by the rapid development of high-frequency water quality measurements in streams and rivers. Finally, we discuss future directions and challenges for using high-frequency water quality measurements to bridge scientific and management gaps by promoting a holistic understanding of freshwater systems and catchment status, health, and function.

Long-term annual soil nitrogen surplus across Europe (1850-2019).

Worldwide surface waters suffer from the presence of nitrogen (N) compounds causing eutrophication and deterioration of the water quality. Despite many Europe-wide legislation's, we still observe high N levels across many water bodies in Europe. Information on long-term annual soil N surplus is needed to better understand these N levels and inform future management strategies. Here, we reconstructed and analysed the annual long-term N surplus for both agricultural and non-agricultural soils across Europe at a 5 arcmin (≈10 km at the equator) spatial resolution for more than a century (1850-2019). The dataset consists of 16 N surplus estimates that account for the uncertainties resulting from input data sources and methodological choices in major components of the N surplus. We documented the consistency and plausibility of our estimates by comparing them with previous studies and discussed about possible avenues for further improvements. Importantly, our dataset offers the flexibility of aggregating the N surplus at any spatial scale of relevance to support water and land management strategies.

Seasonality of inundation in geographically isolated wetlands across the United States.

Inundation area is a major control on the ecosystem services provisioned by geographically isolated wetlands. Despite its importance, there has not been any comprehensive study to map out the seasonal inundation characteristics of geographically isolated wetlands over the continental United States (CONUS). This study fills the aforementioned gap by evaluating the seasonality or the long-term intra-annual variations of wetland inundation in ten wetlandscapes across the CONUS. We also assess the consistency of these intra-annual variations. Finally, we evaluate the extent to which the seasonality can be explained based on widely available hydrologic fluxes. Our findings highlight significant intra-annual variations of inundation within most wetlandscapes, with a standard deviation of the long-term averaged monthly inundation area ranging from 15% to 151% of its mean across the wetlandscapes. Stark differences in inundation seasonality are observed between snow-affected vs. rain-fed wetlandscapes. The former usually shows the maximum monthly inundation in April following spring snowmelt (SM), while the latter experiences the maximum in February. Although the magnitude of inundation fraction has changed over time in several wetlandscapes, the seasonality of these wetlands shows remarkable constancy. Overall, commonly available regional hydrologic fluxes (e.g. rainfall, SM, and evapotranspiration) are found to be able to explain the inundation seasonality at wetlandscape scale with determination coefficients greater than 0.57 in 7 out of 10 wetlandscapes. Our methodology and presented results may be used to map inundation seasonality and consequently account for its impact on wetland functions.

Scaling mechanical instabilities in drying micellar droplets.

Drying-induced mechanical instabilities in aqueous solution droplets occur primarily because, during evaporation, the central liquid minimizes the surface tension by pulling the packed gel-like region, leading to a stretching effect of the liquid region at the receding wet front. Under an appropriate scenario, it finally perturbs the gel-like zone at the droplet periphery, generating cracks, wrinkles, folds, cavities, buckles, etc. Here we report unique wrinkling patterns from evaporating sessile micellar aqueous droplets on rigid and soft substrates kept at temperatures well above the ambient. The wrinkling patterns remarkably vary depending on the material's elastic modulus and substrate, the concentration of the micellar solution (CCTAB), and the substrate temperature (TS). In the low concentration regime (CCTAB ≤ 0.0364 wt%), coffee-ring-like morphologies are observed devoid of any wrinkling morphology irrespective of TS and the substrate's elastic modulus. In the high initial concentration regime (CCTAB ≥ 0.0364 wt%), for droplets deposited at TS ≥ 85 °C, wrinkle formation starts at the droplet peripheral zone, radial on the stiff glass substrate, and annular on the soft cross-linked PDMS substrate. At CCTAB ≥ 2.73 wt%, radial wrinkles on the glass substrate and annular wrinkles on the cross-linked PDMS substrate nucleate from the edges connecting to the central region of the deposit. The ratio between the width of the gel-like deposit (or wrinkle length) and the droplet's radius scales with the initial concentration of the surfactant and depends on the initial equilibrium contact angle of the micellar droplets. Our results support existing understandings of mechanical instabilities of dried deposits, which satisfies interdependent scaling relationships among their number, lengthscale (dried deposit radius, the wavelength of the wrinkles, and peripheral undulations from Rayleigh-Bénard instability), thickness, and elastic modulus. Interestingly, we found substrate-dependent antagonistic interdependence of the elastic modulus of the dried deposit with the initial surfactant concentration.

Vulnerable Waters are Essential to Watershed Resilience.

Watershed resilience is the ability of a watershed to maintain its characteristic system state while concurrently resisting, adapting to, and reorganizing after hydrological (for example, drought, flooding) or biogeochemical (for example, excessive nutrient) disturbances. Vulnerable waters include non-floodplain wetlands and headwater streams, abundant watershed components representing the most distal extent of the freshwater aquatic network. Vulnerable waters are hydrologically dynamic and biogeochemically reactive aquatic systems, storing, processing, and releasing water and entrained (that is, dissolved and particulate) materials along expanding and contracting aquatic networks. The hydrological and biogeochemical functions emerging from these processes affect the magnitude, frequency, timing, duration, storage, and rate of change of material and energy fluxes among watershed components and to downstream waters, thereby maintaining watershed states and imparting watershed resilience. We present here a conceptual framework for understanding how vulnerable waters confer watershed resilience. We demonstrate how individual and cumulative vulnerable-water modifications (for example, reduced extent, altered connectivity) affect watershed-scale hydrological and biogeochemical disturbance response and recovery, which decreases watershed resilience and can trigger transitions across thresholds to alternative watershed states (for example, states conducive to increased flood frequency or nutrient concentrations). We subsequently describe how resilient watersheds require spatial heterogeneity and temporal variability in hydrological and biogeochemical interactions between terrestrial systems and down-gradient waters, which necessitates attention to the conservation and restoration of vulnerable waters and their downstream connectivity gradients. To conclude, we provide actionable principles for resilient watersheds and articulate research needs to further watershed resilience science and vulnerable-water management.

Nitrogen legacies in anthropogenic landscapes: a case study in the Mondego Basin in Portugal.

Nitrogen (N) legacies have built up in anthropogenic landscapes over decades of agricultural intensification, and these legacies lead to time lags in water quality change measurable even beyond the moment of application of N. It is important to understand these legacies to quantify the relationship between N inputs and N concentrations in streams and implement best management practices for water quality improvement; however, little is known about the magnitude of legacies in various landscape elements like soils and groundwater. Here, we have used the ELEMeNT (Exploration of Long-tErM Nutrient Trajectories) model to explore the buildup and depletion of N legacies over a 216-year period, across the Mondego River Basin, a 6645-km2 watershed in Portugal, where human interventions have considerably changed the characteristics of the basin to prevent floods and improve farming conditions in recent decades. The results show that the increase in the amount of inorganic fertilizer applied was the main driver for the anthropogenic N loads in the watershed from 1950 until the beginning of the 1990s. The N inputs have been decreasing since then, but N loads in the river did not document any decrease till the 1990s; after which there was a decline. This time lag between the N inputs to the watershed and the N loads in the river (about two decades) is a function of accumulation of N legacy.

Agricultural phosphorus surplus trajectories for Ontario, Canada (1961-2016), and erosional export risk.

Management strategies aimed at reducing nutrient enrichment of surface waters may be hampered by nutrient legacies that have accumulated in the landscape. Here, we apply the Net Anthropogenic Phosphorus Input (NAPI) model to reconstruct the historical phosphorus (P) input trajectories for the province of Ontario, which encompasses the Canadian portion of the drainage basin of the Laurentian Great Lakes (LGL). NAPI considers P inputs from detergent, human and livestock waste, fertilizer inputs, and P outputs by crop uptake. During the entire time period considered, from 1961 to 2016, Ontario experienced positive annual NAPI values. Despite a generally downward NAPI trend since the late 1970s, the lower LGL, especially Lake Erie, continue to be plagued by algal blooms. When comparing NAPI results and river monitoring data for the period 2003 to 2013, P discharged by Canadian rivers into Lake Erie only accounts for 12.5% of the NAPI supplied to the watersheds' agricultural areas. Thus, over 85% of the agricultural NAPI is retained in the watersheds where it contributes to a growing P legacy, primarily as soil P. The slow release of legacy P therefore represents a long-term risk to the recovery of the lake. To help mitigate this risk, we present a methodology to spatially map out the source areas with the greatest potential of erosional export of legacy soil P to surface waters. These areas should be prioritized in soil conservation efforts.

Cationic surfactant-directed structural control of NaCl crystals from evaporating sessile droplets.

We report morphological regulation of NaCl (sodium chloride) crystals through the evaporative crystallisation process of microdroplets containing a cationic surfactant CTAB (cetyltrimethylammonium bromide). Various fascinating evaporative salt morphologies are observed using different combinations of salt (CNaCl) and surfactant (CCTAB) concentrations. Each observed morphology is carefully explained by the interplaying physical phenomena, such as crystallisation, micellisation, evaporative dewetting, and surface adsorption of anionic couneterions. Salt morphologies are investigated for low (CNaCl = 0.1 (M)), intermediate (CNaCl = 0.5 (M)) and high (CNaCl = 2 (M)) concentrations, whereas surfactant concentrations are varied four orders of magnitudes (from 0.0001 (M) to 0.1 (M)). Interestingly, we observe a threshold in CCTAB at 0.001 (M), beyond which the peripheral rings of dried deposits are found to be composed of CTAB for CNaCl = 0.1 (M), while the same is seen to be made up of NaCl for CNaCl = 2 (M). We have explained the morphological evolution by the process of competitive surface adsorption phenomenon between Cl- and Br- counter ions. Such a detailed study of saline droplet crystallisation in the presence of a cationic surfactant underpins the fundamental understanding of the crystallisation process. In addition, it may further impact application sectors where crystallisation of saline solution plays an important role, especially in the presence of additives.

Great Lakes Runoff Intercomparison Project Phase 3: Lake Erie (GRIP-E).

Hydrologic model intercomparison studies help to evaluate the agility of models to simulate variables such as streamflow, evaporation, and soil moisture. This study is the third in a sequence of the Great Lakes Runoff Intercomparison Projects. The densely populated Lake Erie watershed studied here is an important international lake that has experienced recent flooding and shoreline erosion alongside excessive nutrient loads that have contributed to lake eutrophication. Understanding the sources and pathways of flows is critical to solve the complex issues facing this watershed. Seventeen hydrologic and land-surface models of different complexity are set up over this domain using the same meteorological forcings, and their simulated streamflows at 46 calibration and seven independent validation stations are compared. Results show that: (1) the good performance of Machine Learning models during calibration decreases significantly in validation due to the limited amount of training data; (2) models calibrated at individual stations perform equally well in validation; and (3) most distributed models calibrated over the entire domain have problems in simulating urban areas but outperform the other models in validation.

The need to integrate legacy nitrogen storage dynamics and time lags into policy and practice.

Increased fluxes of reactive nitrogen (Nr), often associated with N fertilizer use in agriculture, have resulted in negative environmental consequences, including eutrophication, which cost billions of dollars per year globally. To address this, best management practices (BMPs) to reduce Nr loading to the environment have been introduced in many locations. However, improvements in water quality associated with BMP implementation have not always been realised over expected timescales. There is a now a significant body of scientific evidence showing that the dynamics of legacy Nr storage and associated time lags invalidate the assumptions of many models used by policymakers for decision making regarding Nr BMPs. Building on this evidence, we believe that the concepts of legacy Nr storage dynamics and time lags need to be included in these models. We believe the biogeochemical research community could play a more proactive role in advocating for this change through both awareness raising and direct collaboration with policymakers to develop improved datasets and models. We anticipate that this will result in more realistic expectations of timescales for water quality improvements associated with BMPs. Given the need for multi-nutrient policy responses to tackle challenges such as eutrophication, integration of N stores will have the further benefit of aligning both researchers and policymakers in the N community with the phosphorus and carbon communities, where estimation of stores is more widespread. Ultimately, we anticipate that integrating legacy Nr storage dynamics and time lags into policy frameworks will better meet the needs of human and environmental health.

Diagnostic accuracy of commercially available immunochromatographic rapid tests for diagnosis of dengue in India.

BACKGROUND & OBJECTIVES: There is limited evidence regarding the accuracy of dengue rapid diagnostic kits despite their extensive use in India. We evaluated the performance of four immunochromatographic Rapid Diagnostic Test (RDTs) kits: Multisure dengue Ab/Ag rapid test (MP biomedicals; MP), Dengucheck combo (Zephyr Biomedicals; ZB), SD bioline dengue duo (Alere; SD) and Dengue day 1 test (J Mitra; JM). METHODS: This is a laboratory-based diagnostic evaluation study. Rapid tests results were compared to reference non-structural (NS1) antigen or immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) results of 241 dengue-positive samples and 247 dengue-negative samples. Sensitivity and specificity of NS1 and IgM components of each RDT were calculated separately and in combination (either NS1 or IgM positive) against reference standard ELISA. RESULTS: A total of 238, 226, 208, and 146 reference NS1 ELISA samples were tested with MP, ZB, SD, and JM tests, respectively. In comparison to the NS1 ELISA reference tests, the NS1 component of MP, ZB, SD, and JM RDTs demonstrated a sensitivity of 71.8%, 85.1%, 77.2% and 80.9% respectively and specificity of 90.1%, 92.8%, 96.1 %, and 93.6%, respectively. In comparison to the IgM ELISA reference test, the IgM component of RDTs showed a sensitivity of 40.0%, 50.3%, 47.3% and 20.0% respectively and specificity of 92.4%, 88.6%, 96.5%, and 92.2% respectively. Combining NS1 antigen and IgM antibody results led to sensitivities of 87.5%, 82.9%, 93.8% and 91.7% respectively, and specificities of 75.3%, 73.9%, 76.5%, and 80.0% respectively. INTERPRETATION & CONCLUSION: Though specificities were acceptable, the sensitivities of each test were markedly lower than manufacturers' claims. These results also support the added value of combined antigen-and antibody-based RDTs for the diagnosis of acute dengue.

Evaporative Drying of Sodium Chloride Solution Droplet on a Thermally Controlled Substrate.

We report the evaporative drying-mediated pattern formation of dilute aqueous NaCl solution droplets on a hydrophobic surface made of cross-linked PDMS at elevated temperatures. Experiments were performed with various salt concentrations (0.08 to 2 M). Depending on the substrate temperature (TS) and initial salt concentration (Cn), a variety of unique deposition morphologies are obtained. Double salt rings are obtained at low Cn (0.08 and 0.1 M) at TS ≈ 75 °C. With the increase in Cn, the deposition morphology transforms into a single peripheral ring with a central deposition zone that is devoid of any salt deposition. On the other hand, at lower Cn and lower TS, a single outer ring deposit surrounding a central region covered with dendritic salt deposits is observed. The variation in the morphology as well as the number of rings formed is attributed to the relative strengths of the radially outward capillary-driven flow, inward Marangoni flow, and inward diffusive transport of the salt ions. A morphology phase diagram shows the collective dependence of the final deposition pattern as a function of both TS and Cn.

Prevalence of kdr mutations and insecticide susceptibility among natural population of Aedes aegypti in West Bengal.

BACKGROUND: Aedes albopictus and Aedes aegypti are the major vectors of arboviral diseases. As effective vaccines are not available for most of the arboviral diseases, vector control by using insecticides play the key role to reduce the disease transmission. The emergence and spread of resistance to different classes of insecticides by the vectors is a major obstacle to control the disease transmission. Information about vector susceptibility to different insecticides and their mechanisms are very important for formulating proper vector control measures. The present study was designed to assess the susceptibility of Ae. aegypti against three different classes of adulticides, one larvicidal agent available and polymorphisms in the voltage-gated sodium channel (VGSC) gene related to insecticide resistance. METHODS: Immature stages of Ae. aegypti were collected from three dengue endemic municipal areas of West Bengal and reared in the laboratory. Larvae and adults (F1 progeny) were used for insecticide bioassay as per WHO protocols. Knock down resistance gene (kdr) mutations were assessed by direct sequencing of PCR products. RESULTS: The Ae. aegypti population was found to be susceptible to type II pyrethroids and malathion but highly resistant to DDT. A high rate of polymorphisms in the VGSC gene was observed among the collected mosquitoes. A double mutant V1016G + F1534C was found to be associated with DDT resistance but neither V1016G nor F1534C alone showed the same association. Association between the kdr mutations and the susceptibility status of pyrethroids could not be established due to very small sample size. A low to moderate level of resistance was noticed against temephos among the larval population based on WHO criteria. CONCLUSION: The replacement of DDT by type II pyrethroids for the management of dengue vectors is an appropriate decision taken by the national program which is supported by the findings of a higher level of resistance to DDT. Persistence of polymorphisms in the VGSC gene might be an indication of emergence of resistance against pyrethroid insecticides that should be monitored at a regular interval. Attempts should be made to determine the effectiveness of other larvicides for replacement of temephos if needed in future. Along with the chemical insecticides different biological vector control methods as well as biopesticides should also be used in vector control programmes.

Turnover and legacy of sediment-associated PAH in a baseflow-dominated river.

Polycyclic Aromatic Hydrocarbons (PAH) ubiquitously occur in rivers and threaten the aquatic ecosystem. Understanding their fate and behaviour in rivers can help in improving management strategies. We develop a particle-facilitated transport model considering suspended sediments with sorbed PAH from different origins to investigate the turnover and legacy of sediment-bound PAH in the baseflow-dominated Ammer River in southwest Germany. Our model identifies the contributions of dissolved and particle-bound PAH during wet and dry periods to the annual load. The analysis of in-stream processes enables investigating the average turnover times of sediments and attached PAH for the main stem of the river. The legacy of sediment-bound PAH is studied by running the model assuming a 50% reduction in PAH emissions after the introduction of environmental regulation in the 1970s. Our results show that sediment-bound and dissolved PAH account for 75% and 25% of the annual PAH load, respectively. PAH are mainly emitted from urban areas that contribute over 74% to the total load. In steep reaches, the turnover times of sediments and attached PAH are similar, whereas they differ by 1-2 orders of magnitude in reaches with very mild slopes. Flow rates significantly affect PAH fluxes between the mobile water and the riverbed over the entire river. Total PAH fluxes from the river bed to the mobile water are simulated to occur when the discharge is larger than 5 m3s -1. River segments with large sediment storage show a potential of PAH legacy, which may have caused a PAH release over 10-20 years after the implementation of environmental regulation. This study is useful for assessing environmental impacts of PAH in rivers (e.g., their contribution to the river-water toxicity) and exemplifies that the longitudinal distribution, turnover, and legacy potential of PAH in a river system require a mechanistic understanding of river hydraulics and sediment transport.

T11TS immunotherapy augments microglial and lymphocyte protective immune responses against Cryptococcus neoformans in the brain.

Cryptococcus neoformans, the encapsulated yeast acquired through inhalation, remains localized in lungs, but harbours the CNS in immunocompromised individuals. Several treatment regimes have failed combating this disease totally, but long-term usage of drugs leads to organ damage. As T11-target structure (T11TS) has documented profound immune potentiation, we aimed to investigate the role of microglia, pivotal immune cells of brain in ameliorating cryptococcosis, with T11TS immunotherapy. Murine model with C neoformans infection was prepared by intraperitoneal injection and the brains of rats examined 7 days post-infections for histopathology by PAS and Alcian blue staining corroborated with organ fungal burden evidencing restorative T11TS action on Cryptococcal meningitis. Immunotherapy with three doses of T11TS, a CD2 ligand, in C neoformans infected rats, upregulates toll-like receptors 2, -4 and -9 of microglia, indicating increased phagocytosis of the fungus. Flowcytometric analysis revealed increased numbers of T11TS treated brain infiltrating CD4+ and CD8+ T-lymphocytes along with increased MHC I and MHC II on microglia, activating the infiltrating lymphocytes aiding the killing mechanism. Present study also indicated that T11TS increased production of Th1 inflammatory cytokines conducive to fungal elimination while the inhibitory Th2 cytokines were dampened. This preclinical study is first of its kind to show that T11TS effected profound immune stimulation of microglial activity of C neoformans infected rats eradicating residual fungal burden from the brain and can be a useful therapeutic strategy in fighting against this deadly disease.

Morphology modulation in evaporative drying mediated crystallization of sodium chloride solution droplet with surfactant.

We report the evaporative drying of an aqueous droplet containing a dilute solution of sodium chloride (NaCl) on a hydrophobic substrate made of cross-linked poly-dimethyl siloxane (PDMS). The salt concentration Cn was varied between 0.08 molar (M) and 2.0 M. The contact line of the evaporating droplets shows significant initial retraction for all Cn, before they get pinned. While the final morphology comprises a few small NaCl crystals deposited around the pinned contact line, in droplets with a low Cn (<0.5 M), it transforms to a single large salt crystal when Cn > 0.7 M with no peripheral deposition. We further show that the deposition morphology drastically changes when an anionic surfactant, sodium dodecyl sulfate (SDS), is added into the salt-solutions. Even in the surfactant-laden droplets, the final deposition morphology changes significantly as a function of Cn. It transforms from a thick SDS ring surrounding a fractal-like deposit of NaCl crystallites at lower Cn to a peripheral deposit of NaCl crystals at higher Cn due to competition between micelle formation and crystallization. However, the crystallographic orientation of the deposited NaCl crystals remains unaltered irrespective of the presence of surfactant.

Insecticide susceptibility status of Phlebotomus argentipes and polymorphisms in voltage-gated sodium channel (vgsc) gene in Kala-azar endemic areas of West Bengal, India.

Rational use of insecticides, as advocated by World Health Organisation, plays a crucial role for vector control in eliminating visceral leishmaniasis from endemic countries. Emergence and spread of resistance among vector sand flies is of increasing concern for achieving these goals. Information on insecticide susceptibility status of sand fly populations and potential association between the former and polymorphisms in the insecticide target genes is important for formulating proper vector control measures. The present study was designed to evaluate the susceptibility status of vector sand fly species (Phlebotomus argentipes) against deltamethrin (type II pyrethroid), DDT (organochlorine) and malathion (organophosphate) and to detect polymorphisms in voltage gated sodium channel (vgsc) gene and investigating their association with type II pyrethroid and DDT susceptibility in three Kala-azar endemic districts of West Bengal, India. Adult sand flies were collected from human dwelling and cattle sheds of the study areas and subjected to insecticide bioassay using insecticide impregnated papers as per WHO protocol. Polymorphisms in domain II segment 6 of vgsc gene of pyrethroid and DDT susceptible and tolerant P. argentipes were detected by DNA sequencing. P. argentipes population of the study area was found to be susceptible to deltamethrin and malathion with corrected mortality rate between 98.02% to 98.80% and 98.81% to 100% respectively, but resistant to DDT (corrected mortality rate = 65.62%-76.33%). Two non-synonymous mutations L1014S and L1014F were detected of which L1014F was found to be associated with deltamethrin/DDT resistance. The replacement of DDT by synthetic pyrethroid is aptly done by national vector borne disease control programme (NVBDCP). The prevalence of L1014F mutation in vgsc gene and its association with type II pyrethroid tolerability is an indication of emergence of resistance against it. Malathion may be used as an alternative in the study areas if needed in future. Similar studies at a regular interval are highly suggested for monitoring susceptibility of used insecticide and to detect early signs of emergence of resistance against them.

Polymorphisms in voltage-gated sodium channel gene and susceptibility of Aedes albopictus to insecticides in three districts of northern West Bengal, India.

BACKGROUND: The control and prevention of dengue largely depends on vector control measures, environmental management, and personal protection. Dengue control programmes are facing great challenges due to development of insecticide resistance among vector mosquitoes. Information on susceptibility status to different insecticides is important for national programmes to formulate vector control strategies. METHODS: We have studied the larval susceptibility of Aedes albopictus to temephos and adult susceptibility to 4% DDT, 0.05% deltamethrin, and 5% malathion as per WHO protocols in the northern districts of West Bengal. Polymorphisms in the VGSC gene were studied by direct sequencing of PCR products. RESULTS: The Ae. albopictus larval population showed sensitive [Resistance Ratio (RR99)<3] to moderate levels of resistance (510) to temephos at different study sites. Adult bioassay results revealed that Ae. albopictus was highly resistant to DDT [Corrected Mortality (CM) < 90%] in all the study sites and susceptible to deltamethrin and malathion (CM > 98%), except in Dhupguri where a low level of resistance to deltamethrin (CM = 96.25%) was recorded. None of the six important kdr mutations (S953P, I975M/V, L978, V980G, F1474C, D1703Y) were found in the VGSC of studied mosquitoes, but we identified 11 synonymous and 1 non-synonymous mutation in the VGSC gene. CONCLUSION: The higher susceptibility level to deltamethrin and malathion, along with the absence of important kdr mutations indicates that these two insecticides are still effective against Ae. albopictus in the study areas. The susceptibility status of temephos should be monitored closely as low to moderate levels of resistance were observed in few sites. A similar study is recommended for monitoring and early detection of insecticide resistance in other parts of the country.