Biogenic silver nanoparticles and cinnamaldehyde as an effective sanitizer for fresh sweet grape tomatoes.

This study evaluated the antibacterial activity of cinnamaldehyde (CIN) and biogenic silver nanoparticles (BioAgNP), alone and in combination, against Escherichia coli, Salmonella Typhimurium, and Staphylococcus aureus in vitro. Their sanitation activities on fresh sweet grape tomatoes were also evaluated. CIN and BioAgNP inhibited the growth of the tested bacteria, and at low concentrations, their combinations presented a synergistic effect. In the sanitization of fresh sweet grape tomatoes, CIN (156 µg/mL) combined with BioAgNP (31.25 µM) at subinhibitory concentrations inhibited the growth of E. coli after only 5 min of contact. Exposed samples showed no growth of E. coli during their shelf life. The combination of these compounds did not change significantly (p > 0.05) the physicochemical properties of sweet grape tomatoes and showed that CIN combined with BioAgNP could represent an effective method for decontaminating fruits and vegetables. This combination has great potential for application in the prevention of foodborne diseases.

Synergistic inhibition of Salmonella Typhimurium and Staphylococcus aureus in apple jam by cinnamaldehyde and potassium sorbate.

The objective of this study was to evaluate the antimicrobial effectiveness of cinnamaldehyde (CIN) and potassium sorbate (P.S.), alone and in combination, against Salmonella Typhimurium and Staphylococcus aureus in vitro and in apple jam. Antimicrobial activity in vitro was investigated by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), time-kill assay and determination of fractional inhibitory concentration index. CIN MIC and MBC was 312 µg/mL. P.S. MIC and MBC were 2500 and 5000 µg/mL, respectively, against S. Typhimurium; and 10,000 and 20,000 µg/mL, respectively, against S. aureus. The compounds combined exhibited a synergistic effect (FIC < 0.5), inhibiting S. Typhimurium growth after 12 h and S. aureus after 24 h. The effect of CIN and P.S., at sub-inhibitory concentrations, against bacterial strains in apple jam was evaluated during storage. Physicochemical and sensory analyses were also performed. No cultivable S. Typhimurium or S. aureus cells were recovered in apple jam supplemented with CIN + P.S. on the third day of storage. The addition of CIN and P.S. did not affect the physicochemical properties and sensory evaluation showed a score above 7.0. CIN and P.S. association at sub-inhibitory concentrations was effective in controlling foodborne pathogens and improved the shelf life of apple jam.

Psoas muscle mass index as a predictor of long-term mortality and severity of complications after major intra-abdominal colorectal surgery - A retrospective analysis.

STUDY OBJECTIVE: Determine if psoas muscle area measured in routine preoperative computed tomography scans (CT) can be used to identify patients at increased risk of adverse postoperative outcomes after major elective abdominal surgery. DESIGN: Retrospective analysis of data from a single-centre cohort study conducted in York Hospital between the 1st August of 2015 and the 31st of august of 2020. SETTING: Preoperative clinic. PATIENTS: 639 patients who attended the preoperative assessment clinic prior to major elective colorectal surgery and had an abdominal CT scan done up to 120 days before surgery. INTERVENTIONS: None. MEASUREMENTS: Psoas muscle area at the L3 level was measured in preoperative CT scans and normalised to patient height (psoas muscle index). The lowest sex-stratified tertile of psoas muscle index (PMI) was classed as sarcopenic. The primary outcome was 2-year mortality. Secondary outcomes included postoperative complications assessed using Clavien-Dindo graded major and minor complications, comprehensive complication index (CCI), and length of stay. MAIN RESULTS: Multivariable regression analysis showed that sarcopenia was associated with 2-year mortality (aOR 1.79, 95% CI 1.03-3.10; p = 0.037) and survival at 2-years was significantly reduced in sarcopenic patients (log-rank test, p = 0.012). Sarcopenia was the only statistically significant predictor of major complications in multivariable logistic regression analysis (aOR 1.69, 95% CI 1.04-2.74, p = 0.034) and associated with an estimated increase of 16.6% in the comprehensive complication index (CCI) score of patients that had complications in multivariable linear regression analysis. Sarcopenia was not associated with length of stay. CONCLUSIONS: Sarcopenia defined by psoas muscle mass is an independent predictor of 2-year mortality, major complications and severity of complications after major colorectal surgery and may be used for preoperative risk assessment.

Synergistic activity of Stryphnodendron adstringens and potassium sorbate against foodborne bacteria.

Stryphnodendron adstringens is a medicinal plant that has a broad spectrum of action, including antibacterial activity. The aim of the present study was to evaluate the effect of S. adstringens alone and in combination with potassium sorbate (PS) against foodborne bacteria. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined and, for most of the bacteria tested, the crude extract (CE), aqueous fraction (AQF), and ethyl-acetate fraction (EAF) of S. adstringens had a MIC and MBC ranging from 500 to ≥ 1000 µg/mL. The AQF and EAF showed greater activity against S. aureus strains (MIC = 125 to 250 µg/mL; MBC = 500 to 1000 µg/m). Quantitative cell viability was determined and was observed reductions ranging from 3.0 to 5.8 log10 CFU/ml.The combination of S. adstringens and PS against seven S. aureus isolates was determined by the checkerboard method at neutral and acid pH. In a neutral medium, the AQF + PS combination presented synergistic or additive interactions against six S. aureus strains. The combination of EAF + PS resulted in additive interactions against four bacterial isolates. In an acidic medium, the AQF + PS combination was synergistic or additive against all S. aureus, while EAF + PS presented the same effect against six S. aureus strains S. adstringens showed important antibacterial effects against foodborne S. aureus strains. Moreover, the combination of S. adstringens fractions and PS improved the antibacterial activity compared to the compounds utilized individually. The combined use of these compounds may be an alternative to reduce bacterial food contamination and improve food safety.

Botanical authentication of globe artichoke-containing foods: Differentiation of Cynara scolymus by a novel HRM approach.

Cynara scolymus L., known as globe artichoke, is a medicinal plant widely used in plant food supplements (PFS) and herbal infusions due to its beneficial health properties. The high demand for artichoke-containing products can lead to adulteration practices. In this work, a real-time polymerase chain reaction (PCR) system coupled to high-resolution melting (HRM) analysis was proposed to differentiate C. scolymus from other Cynara species. Hence, a Cynara-specific real-time PCR assay was successfully developed with high analytical performance, achieving a sensitivity of 0.4 pg of globe artichoke DNA. HRM analysis enabled the discrimination of C. scolymus, with a high level of confidence (>98%), corroborating sequencing data. Application results to artichoke-containing PFS and mixed herbal infusions allowed confirming the presence of C. scolymus in 38% of the samples, suggesting the substitution/mislabelling of globe artichoke in 2 samples and the need for further efforts to increase DNA amplifiability of PFS.

Corrigendum: Object-Based Analyses in FIJI/ImageJ to Measure Local RNA Translation Sites in Neurites in Response to Aß1-42 Oligomers.

[This corrects the article DOI: 10.3389/fnins.2020.00547.].

pH interferes in photoinhibitory activity of curcumin nanoencapsulated with pluronic® P123 against Staphylococcus aureus.

Microbial contamination control is a public health concern and challenge for the food industry. Antimicrobial technologies employing natural agents may be useful in the food industry for these purposes. This work aimed to investigate the effect of photodynamic inactivation using curcumin in Pluronic® P123 nanoparticles (Cur/P123) at different pH and blue LED light against Staphylococcus aureus. Bacterial photoinactivation was conducted using different photosensitizer concentrations and exposure times at pH 5.0, 7.2 and 9.0. A mixture design was applied to evaluate the effects of exposure time (dark and light incubation) on the photoinhibitory effect. S. aureus was completely inactivated at pH 5.0 by combining low concentrations of Cur/P123 (7.80-30.25 µmol/L) and light doses (6.50-37.74 J/cm2). According to the mathematical model, dark incubation had low significance in bacterial inactivation at pH 5.0 and 9.0. No effect in bacterial inactivation was observed at pH 7.2. Cur/P123 with blue LED was effective in inactivating S. aureus. The antimicrobial effect of photodynamic inactivation was also pH-dependent.

Object-Based Analyses in FIJI/ImageJ to Measure Local RNA Translation Sites in Neurites in Response to Aß1-42 Oligomers.

Subcellular protein delivery is especially important in signal transduction and cell behavior, and is typically achieved by localization signals within the protein. However, protein delivery can also rely on localization of mRNAs that are translated at target sites. Although once considered heretical, RNA localization has proven to be highly conserved in eukaryotes. RNA localization and localized translation are especially relevant in polarized cells like neurons where neurites extend dozens to hundreds of centimeters away from the soma. Local translation confers dendrites and axons the capacity to respond to their environment in an acute manner without fully relying on somatic signals. The relevance of local protein synthesis in neuron development, maintenance and disease has not been fully acknowledged until recent years, partly due to the limited amount of locally produced proteins. For instance, in hippocampal neurons levels of newly synthesized somatic proteins can be more than 20-30 times greater than translation levels of neuritic proteins. Thus local translation events can be easily overlooked under the microscope. Here we describe an object-based analysis used to visualize and quantify local RNA translation sites in neurites. Newly synthesized proteins are tagged with puromycin and endogenous RNAs labeled with SYTO. After imaging, signals corresponding to neuritic RNAs and proteins are filtered with a Laplacian operator to enhance the edges. Resulting pixels are converted into objects and selected by automatic masking followed by signal smoothing. Objects corresponding to RNA or protein and colocalized objects (RNA and protein) are quantified along individual neurites. Colocalization between RNA and protein in neurites correspond to newly synthesized proteins arising from localized RNAs and represent localized translation sites. To test the validity of our analyses we have compared control neurons to Aß1 - 42-treated neurons. Aß is involved in the pathology of Alzheimer's disease and was previously reported to induce local translation in axons and dendrites which in turn contributes to the disease. We have observed that Aß increases the synthesis of neuritic proteins as well as the fraction of translating RNAs in distal sites of the neurite, suggesting an induction of local protein synthesis. Our results thus confirm previous reports and validate our quantification method.

Application of Response Surface Methodology to Evaluate Photodynamic Inactivation Mediated by Eosin Y and 530 nm LED against Staphylococcus aureus.

Photodynamic antimicrobial chemotherapy (PAC) is an efficient tool for inactivating microorganisms. This technique is a good approach to inactivate the foodborne microorganisms, which are responsible for one of the major public health concerns worldwide-the foodborne diseases. In this work, response surface methodology (RSM) was used to evaluate the interaction of Eosin Y (EOS) concentration and irradiation time on Staphylococcus aureus counts and a sequence of designed experiments to model the combined effect of each factor on the response. A second-order polynomial empirical model was developed to describe the relationship between EOS concentration and irradiation time. The results showed that the derived model could predict the combined influences of these factors on S. aureus counts. The agreement between predictions and experimental observations (R2adj = 0.9159, p = 0.000034) was also observed. The significant terms in the model were the linear negative effect of photosensitizer (PS) concentration, followed by the linear negative effect of irradiation time, and the quadratic negative effect of PS concentration. The highest reductions in S. aureus counts were observed when applying a light dose of 9.98 J/cm2 (498 nM of EOS and 10 min. irradiation). The ability of the evaluated model to predict the photoinactivation of S. aureus was successfully validated. Therefore, the use of RSM combined with PAC is a promising approach to inactivate foodborne pathogens.

Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study.

Carvacrol presents action in Salmonella Typhimurium biofilms, however the antibiofilm mechanism of this compound has not been fully established yet. In the present study, the aim was to evaluate protein profile changes in S. Typhimurium biofilm treated with carvacrol. Proteomic analysis of treated versus untreated biofilm showed several changes in proteins involved with S. Typhimurium biofilm and antioxidant activity. The proteins DsbA (thiol: disulfide interchange protein DsbA), LuxS (S-ribosylhomocysteine lyase), DksA (RNA polymerase binding transcription factor DksA), and SODs (superoxide dismutases) A, B and C had their synthesis decreased after treatment with carvacrol. These proteins play a key role in S. Typhimurium biofilm formation, demonstrating the dynamic antibiofilm action of carvacrol. The differentially expressed proteins identified provide possible action targets for future studies in order to gain more insight into the mechanism of action of carvacrol on S. Typhimurium biofilm.

Baixas na linha de frente: absenteísmo entre bombeiros durante o combate à pandemia da COVID-19 / Frontline losses: absenteeism among firefighters during the fight against the COVID-19 pandemic

Resumo Objetivos: descrever o absenteísmo relacionado a casos suspeitos (com infecção respiratória aguda) e confirmados da COVID-19 e a outros diagnósticos entre bombeiros de Minas Gerais, Brasil. Métodos: foram analisados os registros oficiais sobre afastamento do trabalho por problemas de saúde (licença-saúde). A análise foi organizada em: 1) gráficos estratificados por grupo de diagnóstico; 2) análises descritivas da proporção de licenças-saúde e do percentual de dias de trabalho perdidos por infecção respiratória aguda; 3) comparação (qui-quadrado) da proporção de licenças-saúde e do percentual de dias de trabalho perdidos por infecção respiratória aguda e por outros diagnósticos entre 2019 e 2020. Resultados: a análise gráfica mostrou um padrão regular de licenças-saúde antes do início da pandemia, um pico de licenças-saúde por infecção respiratória aguda após o início da pandemia e um novo padrão de licenças-saúde após o período de pico. A proporção de licenças-saúde e o percentual de dias de trabalho perdidos por infecção respiratória aguda aumentaram, respectivamente, 312% e 580% em 2020. Em contraste, o percentual de dias de trabalho perdidos por outros diagnósticos diminuiu 16%. Conclusão: a mudança no perfil de absenteísmo entre bombeiros reflete o vínculo formal de emprego, as políticas institucionais e a percepção de risco sobre a COVID-19.

Abstract Objectives: to describe absenteeism related to suspected (acute respiratory infection cases) and confirmed cases of COVID-19 and other diagnoses among firefighters in Minas Gerais, Brazil. Methods: we analyzed official records of sick leave due to health problems. The analysis was organized into: 1) graphs stratified by diagnostic group; 2) descriptive analyses of proportion of sick leave and percentage of working days lost due to acute respiratory infection; Chi-squared comparison of proportion of sick leave and percentage of working days lost due to acute respiratory infection, and other diagnoses, between 2019 and 2020. Results: the graphical analysis showed a regular sick leave pattern before the onset of the pandemic, a peak in sick leave due to acute respiratory infection after the onset of the pandemic, and a new sick leave pattern after the peak period. The proportion of sick leave and the percentage of working days lost due to acute respiratory infection increased by 312% and 580% in 2020, respectively. In contrast, the percentage of working days lost due to other diagnoses decreased by 16%. Conclusion: the change in the absenteeism profile among firefighters reflects formal employment contract, institutional policies and risk perception about COVID-19.

The Remarkable Effect of Potassium Iodide in Eosin and Rose Bengal Photodynamic Action against Salmonella Typhimurium and Staphylococcus aureus.

Antimicrobial photodynamic therapy (aPDT) has been shown as a promising technique to inactivate foodborne bacteria, without inducing the development of bacterial resistance. Knowing that addition of inorganic salts, such as potassium iodide (KI), can modulate the photodynamic action of the photosensitizer (PS), we report in this study the antimicrobial effect of eosin (EOS) and rose bengal (RB) combined with KI against Salmonella enterica serovar Typhimurium and Staphylococcus aureus. Additionally, the possible development of bacterial resistance after this combined aPDT protocol was evaluated. The combination of EOS or RB, at all tested concentrations, with KI at 100 mM, was able to efficiently inactivate S. Typhimurium and S. aureus. This combined approach allows a reduction in the PS concentration up to 1000 times, even against one of the most common foodborne pathogenics, S. Typhimurium, a gram-negative bacterium which is not so prone to inactivation with xanthene dyes when used alone. The photoinactivation of S. Typhimurium and S. aureus by both xanthenes with KI did not induce the development of resistance. The low price of the xanthene dyes, the non-toxic nature of KI, and the possibility of reducing the PS concentration show that this technology has potential to be easily transposed to the food industry.

Xanthene Dyes and Green LED for the Inactivation of Foodborne Pathogens in Planktonic and Biofilm States.

This study evaluated the rose bengal- and erythrosine-mediated photoinactivation against Salmonella Typhimurium and Staphylococcus aureus planktonic and sessile cells using green LED as a light source. The free-living or 2-day-old biofilm cells were treated with different concentrations of the photosensitizing agents and subjected to irradiation. Only 5 min photosensitization with rose bengal at 25 nmol L-1 and 75 µmol L-1 completely eliminated S. aureus and S. Typhimurium planktonic cells, respectively. Erythrosine at 500 nmol L-1 and 5 min of light exposure also reduced S. aureus planktonic cells to undetectable levels. Eradication of S. aureus biofilms was achieved when 500 µmol L-1 of erythrosine or 250 µmol L-1 of rose bengal was combined with 30 min of irradiation. Scanning electron microscopy allowed the observation of morphological changes in planktonic cells and disruption of the biofilm architecture after photodynamic treatment. The overall data demonstrate that rose bengal and erythrosine activated by green LED may be a targeted strategy for controlling foodborne pathogens in both planktonic and sessile states.

Intra-axonal Synthesis of SNAP25 Is Required for the Formation of Presynaptic Terminals.

Localized protein synthesis is a mechanism for developing axons to react acutely and in a spatially restricted manner to extracellular signals. As such, it is important for many aspects of axonal development, but its role in the formation of presynapses remains poorly understood. We found that the induced assembly of presynaptic terminals required local protein synthesis. Newly synthesized proteins were detectable at nascent presynapses within 15 min of inducing synapse formation in isolated axons. The transcript for the t-SNARE protein SNAP25, which is required for the fusion of synaptic vesicles with the plasma membrane, was recruited to presynaptic sites and locally translated. Inhibition of intra-axonal SNAP25 synthesis affected the clustering of SNAP25 and other presynaptic proteins and interfered with the release of synaptic vesicles from presynaptic sites. This study reveals a critical role for the axonal synthesis of SNAP25 in the assembly of presynaptic terminals.

Intra-axonal protein synthesis in development and beyond.

Proteins can be locally produced in the periphery of a cell, allowing a rapid and spatially precise response to the changes in its environment. This process is especially relevant in highly polarized and morphologically complex cells such as neurons. The study of local translation in axons has evolved from being primarily focused on developing axons, to the notion that also mature axons can produce proteins. Axonal translation has been implied in several physiological and pathological conditions, and in all cases it shares common molecular actors and pathways as well as regulatory mechanisms. Here, we review the main findings in these fields, and attempt to highlight shared principles.

DNA extraction from plant food supplements: Influence of different pharmaceutical excipients.

The consumption of plant food supplements (PFS) has been growing globally, with an increase of misleading labeling and fraudulent practices also being reported. Recently, the use of molecular biology techniques has been proposed to detect botanical adulterations, one of the possible frauds in PFS. However, difficulties in recovering DNA from some PFS samples have been described. Aiming at using DNA-based methods for the unequivocal identification of plant species in PFS, adequate DNA isolation is required. However, PFS often contain pharmaceutical excipients known to have adsorbent properties that might interfere with DNA extraction. Thus, the aim of this work was to assess the effect of different excipients (talc, silica, iron oxide and titanium dioxide) on the recovery/amplification of DNA. For that purpose, known amounts of template maize DNA were spiked either to PFS or to model mixtures of excipients and quantified by real-time PCR. The tested excipients evidenced clear adsorption phenomena that justify the hampering effect on DNA extraction from PFS. The use of either 10% talc or 0.5% dyes completely adsorbed DNA, resulting in negative PCR amplifications. For the first time, pharmaceutical excipients were shown to affect DNA extraction explaining the inability of recovering DNA from some PFS samples in previous studies.

Trabalho, gênero e saúde mental: uma pesquisa quantitativa e qualitativa entre bombeiros / Work, gender, and mental health: a qualitative and quantitative research among fire fighters

Este estudo objetivou avaliar os indicadores de saúde mental dos bombeiros e os fatores que a influenciam, destacando as diferenças entre as mulheres e os homens. Trata-se de um estudo exploratório que articulou métodos quantitativos e qualitativos. Na primeira etapa, avaliou-se através de instrumentos psicológicos a saúde mental geral, depressão, estresse, ansiedade, desesperança, ideação suicida e consumo de álcool. Participaram dessa fase 303 bombeiros, sendo 277 homens e 26 mulheres. Na segunda etapa, as entrevistas foram avaliadas através da análise de conteúdo, feitas com 37 bombeiros, entre os quais 18 eram homens e 19 mulheres. Os indicativos de depressão, estresse, comprometimentos em saúde mental geral prevaleceram para a amostra feminina. Os discursos de homens e de mulheres se organizaram diferentemente, tanto nas queixas sobre o trabalho e a vida pessoal e social, como nas consequências percebidas para a vida e a saúde. Conclui-se que as influências de aspectos do trabalho, do indivíduo e de sua vida social provavelmente contribuem para a saúde mental de bombeiros, de forma não hierárquica e complexa. Da mesma maneira, cada um desses fatores pode influenciar um ao outro dinamicamente(AU)

This study aimed to assess firefighters' mental health indicators and what it was influenced by, highlighting differences between males and females. The study was exploratory and based on qualitative and quantitative methods. Firstly, indicators such as general mental health, depression, stress, anxiety, hopelessness, suicide ideation and alcohol use pattern were measured with the use of valid psychological tests. The sample comprised 303 firefighters: 277 males and 26 females. After that, 37 out of the total were interviewed (18 males and 19 females). Finally, their answers were evaluated using content analysis technique. The psychological indicators showed prevalence of depression, stress and general mental health higher among females than males. The men's and women's answers content were differed on their complaints about work, private and social lives as well as on their consequences perception on health and life at large. These results indicated that either work or private and social life probably affects firefighters' mental health in a non-hierarchical and complex way. Likewise, work, private and social lives probably affect each other dynamically(AU)

Trabalho, gênero e saúde mental: uma pesquisa quantitativa e qualitativa entre bombeiros / Work, gender, and mental health: a qualitative and quantitative research among fire fighters

Este estudo objetivou avaliar os indicadores de saúde mental dos bombeiros e os fatores que a influenciam, destacando as diferenças entre as mulheres e os homens. Trata-se de um estudo exploratório que articulou métodos quantitativos e qualitativos. Na primeira etapa, avaliou-se através de instrumentos psicológicos a saúde mental geral, depressão, estresse, ansiedade, desesperança, ideação suicida e consumo de álcool. Participaram dessa fase 303 bombeiros, sendo 277 homens e 26 mulheres. Na segunda etapa, as entrevistas foram avaliadas através da análise de conteúdo, feitas com 37 bombeiros, entre os quais 18 eram homens e 19 mulheres. Os indicativos de depressão, estresse, comprometimentos em saúde mental geral prevaleceram para a amostra feminina. Os discursos de homens e de mulheres se organizaram diferentemente, tanto nas queixas sobre o trabalho e a vida pessoal e social, como nas consequências percebidas para a vida e a saúde. Conclui-se que as influências de aspectos do trabalho, do indivíduo e de sua vida social provavelmente contribuem para a saúde mental de bombeiros, de forma não hierárquica e complexa. Da mesma maneira, cada um desses fatores pode influenciar um ao outro dinamicamente.

This study aimed to assess firefighters' mental health indicators and what it was influenced by, highlighting differences between males and females. The study was exploratory and based on qualitative and quantitative methods. Firstly, indicators such as general mental health, depression, stress, anxiety, hopelessness, suicide ideation and alcohol use pattern were measured with the use of valid psychological tests. The sample comprised 303 firefighters: 277 males and 26 females. After that, 37 out of the total were interviewed (18 males and 19 females). Finally, their answers were evaluated using content analysis technique. The psychological indicators showed prevalence of depression, stress and general mental health higher among females than males. The men's and women's answers content were differed on their complaints about work, private and social lives as well as on their consequences perception on health and life at large. These results indicated that either work or private and social life probably affects firefighters' mental health in a non-hierarchical and complex way. Likewise, work, private and social lives probably affect each other dynamically.

Estratégias de rastreamento e intervenções breves para problemas relacionados ao abuso de álcool entre bombeiros / Screening and brief intervention strategies for problems related to alcohol abuse among firemen

Efeitos deletérios do abuso do álcool são amplamente conhecidos e descritos. A prevenção ao abuso de álcool é particularmente importante em categorias profissionais específicas, por exemplo, trabalhadores submetidos a situações de risco e estresse constantes, como bombeiros. A prevenção pode ser crucial para reduzir possíveis conseqüências do abuso de álcool nessas categorias profissionais. Neste trabalho, ilustramos o uso de uma abordagem em particular, o programa de Triagem e Intervenção Breve (TIB) para bombeiros de Juiz de Fora, MG. A TIB foi realizada em associação com outras avaliações, com um fluxo de intervenção aos bombeiros. Nossa proposta se mostrou factível e sistematizou o atendimento aos mesmos, de forma que permitiu uma integração deste tipo de procedimento com as atividades do setor de saúde. Sugerimos que a TIB, em se tratando de problemas relacionados ao abuso de álcool, se mostrou eficaz neste contexto.

Deleterious effects of alcohol abuse are widely known and described. Prevention of alcohol abuse is particularly important in specific professional categories, i.e. workers subjected to constant risk and stressful situations, like firemen. Prevention may be crucial to reduce possible consequences arising from alcohol abuse in such professional categories. In the present paper we illustrate the use of a particular approach based on prevention, the Screening and Brief Intervention (TIB), on firemen from Juiz de Fora, MG. The TIB has been applied in association with other evaluation strategies and has proved to be viable and efficient as health care prevention in such context.

Estratégias de rastreamento e intervenções breves para problemas relacionados ao abuso de álcool entre bombeiros / Screening and brief intervention strategies for problems related to alcohol abuse among firemen

Efeitos deletérios do abuso do álcool são amplamente conhecidos e descritos. A prevenção ao abuso de álcool é particularmente importante em categorias profissionais específicas, por exemplo, trabalhadores submetidos a situações de risco e estresse constantes, como bombeiros. A prevenção pode ser crucial para reduzir possíveis conseqüências do abuso de álcool nessas categorias profissionais. Neste trabalho, ilustramos o uso de uma abordagem em particular, o programa de Triagem e Intervenção Breve (TIB) para bombeiros de Juiz de Fora, MG. A TIB foi realizada em associação com outras avaliações, com um fluxo de intervenção aos bombeiros. Nossa proposta se mostrou factível e sistematizou o atendimento aos mesmos, de forma que permitiu uma integração deste tipo de procedimento com as atividades do setor de saúde. Sugerimos que a TIB, em se tratando de problemas relacionados ao abuso de álcool, se mostrou eficaz neste contexto.(AU)

Deleterious effects of alcohol abuse are widely known and described. Prevention of alcohol abuse is particularly important in specific professional categories, i.e. workers subjected to constant risk and stressful situations, like firemen. Prevention may be crucial to reduce possible consequences arising from alcohol abuse in such professional categories. In the present paper we illustrate the use of a particular approach based on prevention, the Screening and Brief Intervention (TIB), on firemen from Juiz de Fora, MG. The TIB has been applied in association with other evaluation strategies and has proved to be viable and efficient as health care prevention in such context.(AU)