Evaluating the effect of low-level laser therapy on healing of tentomized Achilles tendon in streptozotocin-induced diabetic rats by light microscopical and gene expression examinations.

Tendon healing is impaired in individuals diagnosed with diabetes mellitus (DM). According to research, there is considerable improvement in the healing of surgically tenotomized Achilles tendons following low-level laser therapy (LLLT) in non-diabetic, healthy animals. This study uses light microscopic (LM) and semi-quantitative reverse transcription PCR (RT-PCR) analyses to evaluate the ability of LLLT in healing Achilles tendons from streptozotocin-induced diabetic (STZ-D) rats. A total of 88 rats were randomly divided into two groups, non-diabetic and diabetic. DM was induced in the rats by injections of STZ. The right Achilles tendons of all rats were tenotomized 1 month after administration of STZ. Laser-treated rats were treated with a helium-neon (He-Ne) laser that had a 632.8-nm wavelength and 7.2-mW average power. Experimental group rats received a daily dose of 0.014 J (energy density, 2.9 J/cm(2)). Control rats did not receive LLLT. Animals were sacrificed on days 5, 10, and 15 post-operatively for semi-quantitative LM and semi-quantitative RT-PCR examinations of transforming growth factor-beta1 (TGF-ß1) gene expression. The chi-square test showed that LLLT significantly reduced inflammation in non-diabetic rats compared with their non-diabetic controls (p = 0.02). LLLT significantly decreased inflammation in diabetic rats on days 5 (p = 0.03) and 10 (p = 0.02) compared to the corresponding control diabetic rats. According to the student's t test, LLLT significantly increased TGF-ß1 gene expression in healthy (p = 0.000) and diabetic (p = 0.000) rats compared to their relevant controls. The He-Ne laser was effective in altering the inflammatory reaction and increasing TGF-ß1 gene production.

Histological and gene expression analysis of the effects of pulsed low-level laser therapy on wound healing of streptozotocin-induced diabetic rats.

Diabetes mellitus (DM) is associated with poor wound healing. Studies have shown accelerated wound healing following pulsed low-level laser therapy (LLLT) in non-diabetic animals. The present study aims to evaluate the effect of pulsed LLLT on wound healing in streptozotocin-induced diabetic (STZ-D) rats. We divided 48 rats into two groups of non-diabetic and diabetic. Type 1 DM was induced in the diabetic rat group by injections of STZ. Two, full-thickness skin incisions were made on the dorsal region of each rat. One month after the STZ injection, wounds of the non-diabetic and diabetic rats were submitted to a pulsed, infrared 890-nm laser with an 80-Hz frequency and 0.2 J/cm(2) for each wound point. Control wounds did not receive LLLT. Animals were sacrificed on days 4, 7, and 15 post-injury for histomorphometry and reverse transcription polymerase chain reaction (RT-PCR) analyses of basic fibroblast growth factor (bFGF) gene expression. Pulsed LLLT significantly increased the numbers of macrophages, fibroblasts, and blood vessel sections compared to the corresponding control groups. Semi-quantitative analysis of bFGF gene expression at 48 h post-injury revealed a significant increase in gene expression in both non-diabetic and diabetic rats following LLLT (the ANOVA test). Pulsed LLLT at 0.2 J/cm(2) accelerated the wound healing process in both non-diabetic and diabetic rats as measured by histological characteristics and semi-quantitative bFGF gene expression.

Accommodative changes after photorefractive keratectomy in myopic eyes.

PURPOSE: To determine the changes of amplitude of accommodation (AA) and facility of accommodation (FA) in myopic patients after photorefractive keratectomy (PRK). METHODS: Using Technolas 217Z excimer laser, 160 myopic eyes of 80 patients underwent PRK. The patients were categorized into two age groups: <30 and ≥30 years. Changes in AA and FA were observed before PRK and at 2 weeks, 1 month, and 3 months after PRK. The role of preoperative AA, FA, refractive spherical equivalent, age, and sex on postoperative AA and FA was evaluated. RESULTS: In younger patients, the preoperative AA and FA values 7.77 ± 1.75 D and 7.75 ± 3.97 cpm changed to 8.36 ± 1.26 D and 11.57 ± 4.20 cpm (p < 0.001), respectively, at 3 months after PRK. In older patients, the preoperative AA and FA values 6.66 ± 1.41 D and 5.05 ± 3.26 cpm changed to 6.72 ± 1.26 D (p = 1.000) and 9.58 ± 4.29 cpm (p < 0.001), respectively. Two weeks after surgery, preoperative AA and spherical equivalent had a significant effect on postoperative AA, whereas preoperative AA and age had a significant effect on postoperative AA after 3 months (p < 0.001). Postoperative FA was positively related to preoperative FA and female sex (p < 0.05). CONCLUSIONS: This study suggests that some of the near-vision problems in younger myopes in early postoperative days after PRK might be due to decrease in AA and FA, which will eventually increase. However, in older patients, despite increase in FA, AA did not change.