Accuracy of periodontitis diagnosis obtained using multiple molecular biomarkers in oral fluids: A systematic review and meta-analysis.

AIM: To determine the accuracy of biomarker combinations in gingival crevicular fluid (GCF) and saliva through meta-analysis to diagnose periodontitis in systemically healthy subjects. METHODS: Studies on combining two or more biomarkers providing a binary classification table, sensitivity/specificity values or group sizes in subjects diagnosed with periodontitis were included. The search was performed in August 2022 through PUBMED, EMBASE, Cochrane, LILACS, SCOPUS and Web of Science. The methodological quality of the articles selected was evaluated using the QUADAS-2 checklist. Hierarchical summary receiver operating characteristic modelling was employed to perform the meta-analyses (CRD42020175021). RESULTS: Twenty-one combinations in GCF and 47 in saliva were evaluated. Meta-analyses were possible for six salivary combinations (median sensitivity/specificity values): IL-6 with MMP-8 (86.2%/80.5%); IL-1ß with IL-6 (83.0%/83.7%); IL-1ß with MMP-8 (82.7%/80.8%); MIP-1α with MMP-8 (71.0%/75.6%); IL-1ß, IL-6 and MMP-8 (81.8%/84.3%); and IL-1ß, IL-6, MIP-1α and MMP-8 (76.6%/79.7%). CONCLUSIONS: Two-biomarker combinations in oral fluids show high diagnostic accuracy for periodontitis, which is not substantially improved by incorporating more biomarkers. In saliva, the dual combinations of IL-1ß, IL-6 and MMP-8 have an excellent ability to detect periodontitis and a good capacity to detect non-periodontitis. Because of the limited number of biomarker combinations evaluated, further research is required to corroborate these observations.

Endodontic and aesthetic management of a conoid tooth with type IIIA dens invaginatus.

BACKGROUND: Dens invaginatus is a dental developmental abnormality caused by an infolding of the enamel organ into the adjacent dental papilla before the calcification of the dental tissues. The clinical presentation of dens invaginatus varies according to its severity, from mild forms, in which we might find a deeper than normal cingulum pit, to severe cases, in which there is a pronounced infolding reaching the apical foramen. The very nature of the invagination favours the contamination from the oral environment to the pulp space and the onset of caries. Therefore, patients might present with symptoms or signs of pulpitis and/or apical periodontitis. The aim of this paper is to present the successful endodontic and restorative management of a conoid shaped upper lateral incisor, affected by dens invaginatus type IIIA. CASE REPORT: A 13-year-old girl was referred to our office for endodontic and aesthetic treatment of the right upper lateral incisor, which was asymptomatic. Clinical, radiographic and photographic examinations were performed. The diagnosis of this tooth was dens invaginatus, Oehlers type IIIA with chronic apical periodontitis. Full isolation and magnification were used to achieve correct endodontic management of the aberrant internal anatomy of the tooth. Ultrasonic tips and a combination of manual and mechanical instruments were used for cleaning and shaping. Warm vertical condensation technique was used for sealing the canal system. As the final restoration it was chosen an indirect composite crown bonded to the tooth surface with minor preparation and using an adhesive technique. Radiographic healing and satisfaction of the aesthetic needs was achieved up to 5 years from completion of treatment. CONCLUSION: This case report shows the successful outcomes of a complex case based on proper diagnosis and correct treatment strategies for arresting the progression of apical periodontitis in challenging internal anatomies, as well as the fulfillment of a young patient aesthetic needs.

Oral mucosal peeling related to dentifrices and mouthwashes: A systematic review.

BACKGROUND: The aim of this systematic review was to summarise the clinical information available about oral mucosal peeling (OMP) and to explore its aetiopathogenic association with dentifrices and mouthwashes. MATERIAL AND METHODS: PICOS outline. Population, subjects diagnosed clinically and/or pathologically. Intervention, exposition to oral hygiene products. Comparisons, patients using products at different concentrations. Outcomes, clinicopathological outcomes (primary) and oral epithelial desquamation (secondary) after use. Study design, any. Exclusion criteria, reports on secondary or unpublished data, in vitro studies. Data were independently extracted by two reviewers. RESULTS: Fifteen reports were selected from 410 identified. Descriptive studies mainly showed low bias risk, experimental studies mostly an "unclear risk". Dentifrices or mouthwashes were linked to OMP, with an unknown origin in 5 subjects. Sodium lauryl-sulphate (SLS) was behind this disorder in 21 subjects, tartar-control dentifrices in 2, and flavouring agents in 1 case. Desquamation extension was linked to SLS concentration. Most cases were painless, leaving normal mucosa after desquamation. Tartar-control dentifrices caused ulcerations more frequently. CONCLUSIONS: OMP management should consider differential diagnosis with oral desquamative lesions, particularly desquamative gingivitis, with a guided clinical interview together with pathological confirmation while discouraging the use of the product responsible for OMP.

Patient-centered web-based information on oral lichen planus: Quality and readability.

BACKGROUND: To assess the readability and quality of web-based information available for patients about oral lichen planus (OLP). MATERIAL AND METHODS: Three major search engines (Google, Bing and Yahoo!) were used to identify websites of particular interest to the study using the search term 'oral lichen planus'. The first 100 sites of each search engine were considered for the study. The quality of the contents was evaluated using the DISCERN instrument. The Flesch-Kinkaid Reading Grade Level (FKRGL) and the Flesh Reading Ease Score (FRES) were used to assess readability. The presence of the Health on the Net (HON) seal was also evaluated. RESULTS: Following the application of the study's exclusion criteria, only 28 websites were compiled for further analysis. The median of the DISCERN instrument was 3 [2-3] which means serious or potentially important shortcoming in the quality of the information. Readability indexes pointed to a high reading difficulty (FRES: 48.14±11.22; FKRGL:11.13±2.90). CONCLUSIONS: The information provided by the Internet to the general public regarding OLP has major deficits in terms of quality, and at the same time is difficult for a comprehensive reading. Further studies are warranted to test well-produced patient-centered information on OLP.

Comparative analysis of the biological effects of the endodontic bioactive cements MTA-Angelus, MTA Repair HP and NeoMTA Plus on human dental pulp stem cells.

AIM: To evaluate the biological effects in vitro of MTA-Angelus (MTA-Ang; Angelus, Londrina, PR, Brazil), MTA Repair HP (MTA-HP; Angelus) and NeoMTA Plus (NeoMTA-P; Avalon Biomed Inc, Bradenton, FL, USA) on human dental pulp stem cells (hDPSCs). METHODOLOGY: Cell viability and cell migration assays were performed using eluates of each material. To evaluate cell morphology and cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analysed by immunocytofluorescence and scanning electron microscopy, respectively. The chemical composition of the materials was determined by energy-dispersive X-ray (EDX), and eluates were analysed by inductively coupled plasma-mass spectrometry (ICP-MS). Statistical analysis was performed with the analysis of variance and Bonferroni or Tukey post-test (α < 0.05). RESULTS: Undiluted MTA-Ang, MTA-HP and NeoMTA-P displayed a significant increase in cell viability greater than that obtained using complete medium alone (control) (*P < 0.05; **P < 0.01; ***P < 0.001). Moreover, a cell migration assay revealed cell migration rates after incubation with extracts of MTA-Ang, MTA-HP and NeoMTA-P that were similar to levels obtained in the control group. In addition, stretched cytoskeletal F-actin fibres were detected in the cells treated with the three material extracts. SEM studies revealed a high degree of cell proliferation and attachment on all three materials. EDX analysis demonstrated similar weight percentages of C, O and Ca in all three materials, whilst other elements such as Al, Si and S were also found. CONCLUSIONS: MTA-Ang, MTA-HP and NeoMTA-P were associated with biological effects on hDPSCs in terms of cell proliferation, morphology, migration and attachment.

Primer premolar maxilar con tres conductos. A propósito de un caso / Maxillary first premolar with three canals. A case report

El éxito en el tratamiento de conductos radiculares va a depender entre otras cosas, del conocimiento de la morfología interna y externa de los dientes. Los primeros premolares maxilares presentan una amplia variación en la configuración de las raíces y los conductos radiculares, que debemos estudiar para el correcto diagnóstico y plan de tratamiento. Presentamos un caso clínico de un primer premolar superior con dos raíces y tres conductos

The success of root canal treatment depends on the knowledge of the internal and external tooth morphology among other things. Maxillary first premolars are said to exhibit large variation in root canal morphology we must know to make a proper diagnosis and treatment plan. This article reports a case of an upper left first premolar with two roots and three Canals

La endodoncia en los pacientes mayores / Endodontics in older patients

La endodoncia en el paciente geriátrico es un procedimiento cada vez más habitual en la clínica odontológica. Debemos, dadas las características médicas y dentales de muchos de estos pacientes mayores, planificar correctamente los procesos instrumentales y prever las posibles dificultades que puedan surgir motivadas por conductos estrechos, cámaras pulpares calcificadas etc. En la instrumentación de estos dientes, someteremos a los instrumentos a alto estrés por torsión, motivado por la amplia superficie de contacto entre el instrumento y las paredes, por lo que será totalmente necesario la realización de una preinstrumentación rotatoria y consideramos que es una buena opción el uso del movimiento recíproco. La preparación de los conductos debe dejar un remanente dentinario suficiente, mantenimiento del foramen apical y conicidad progresiva que facilita la administración de irrigantes a lo largo de la longitud de los conductos y las fuerzas de condensación para la obturación. Como irrigantes utilizamos, de forma general, el hipoclorito de sodio al 5% a lo largo de toda la fase instrumental; y una combinación del hipoclorito con un quelante durante la irrigación preobturación que activaremos con algún dispositivo ultrasónico o de aspiración apical negativa. La obturación la realizaremos con sistemas termoplásticos que nos aportan el mejor sellado tridimensional del sistema de conductos (AU)

Endodontics in geriatric patients is an increasingly common procedure in the dental clinic. Medical and dental characteristics of these patients makes us properly plan our procedures to anticipate possible difficulties as narrow ducts, calcifications etc. Our procedures should provide for the possible difficulties that may arise cause by narrow ducts, calcified pulp chambers, etc. During the instrumentation of these teeth, we submit a high stress of torsion to our instruments, motivated by the wide surface of contact between the instrument and the walls. For these reason, it will be absolutely necessary to make a rotary preinstrumentation, then, for the final instrumentation, we must consider the alternating rotation as a good option. The canal preparation should leave one sufficient dentin remnant, maintenance of the apical foramen and the proper taper to facilitate the administration of irrigants along the length of the ducts and the forces of condensation for obturation. Asirrigants we use, in general, sodium hypochlorite 5% throughout the entire instrumental phase; and a combination of hypochlorite with a chelat or for irrigation preobturation, in this phase we will activate with any ultrasonic device or with apical negative pressure. We will make the obturation with thermoplastic systems that provide us a completely three-dimensional sealing of the root canal system (AU)

Diabetes mellitus and optic atrophy: a study of Wolfram syndrome in the Lebanese population.

Wolfram syndrome (WFS) is a rare hereditary neurodegenerative disorder also known as DIDMOAD (diabetes insipidus, diabetes mellitus, optic atrophy, and deafness). WFS seems to be a heterogeneous disease that has not yet been fully characterized in terms of clinical features and pathophysiological mechanisms because the number of patients in most series was small. In this study we describe 31 Lebanese WFS patients belonging to 17 families; this, to our knowledge, is the largest number of patients reported in one series so far. Criteria for diagnosis of WFS were the presence of insulin-dependent diabetes mellitus and optic atrophy unexplained by any other disease. Central diabetes insipidus was found in 87% of the patients, and sensorineural deafness confirmed by audiograms was present in 64.5%. Other less frequent features included neurological and psychiatric abnormalities, urodynamic abnormalities, limited joint motility, cardiovascular and gastrointestinal autonomic neuropathy, hypergonadotropic hypogonadism in males, and diabetic microvascular disease. New features, not reported in previous descriptions, such as heart malformations and anterior pituitary dysfunction, were recognized in some of the patients and participated in the morbidity and mortality of the disease. Genetic analysis revealed WFS1 gene mutations in three families (23.5%), whereas no abnormalities were detected in mitochondrial DNA. In conclusion, WFS is a devastating disease for the patients and their families. More information about WFS will lead to a better understanding of this disease and hopefully to improvement in means of its prevention and treatment.

[Persistent and hyperplastic primary vitreous syndrome. Clinical and therapeutic aspects]. / Syndrome de persistance et d'hyperplasie du vitré primaire. Aspects cliniques et thérapeutiques.

PURPOSE: Persistent hyperplastic primary vitreous (PHPV) is a rare developmental malformation of the eye. This anomaly is usually unilateral and unassociated with other disease. PHPV may have clinical variations: an anterior and/or a posterior one. Diagnosis and treatment are exposed in our paper. METHODS: We study 38 eyes in 34 patients. All patients had an anterior form of PHPV. Nine of them associated a posterior form. Eighteen eyes underwent surgery, the mean postoperative follow-up is 4.7 years. Treatment is discussed, depending on the initial form of PHPV. We report the evolution of the surgical eyes and the untreated eyes. RESULTS: Slit lamp examination and echographic findings (A-scan and B-scan) support the diagnosis. In some cases computed tomography or RMN can be useful. If the cataract is mild in the anterior form of PHPV, treatment of amblyopia and frequent clinical examination can be sufficient. If the cataract is dense, a lensectomy must to be performed. The surgical technique can be difficult. For the eyes with posterior PHPV, the surgical treatment is to be avoided, because of high risk of retinal detachment. CONCLUSION: PHPV may have clinical variations, mostly with cataract as major sign. In purely anterior presentation, in absence of marked microphthalmos, lensectomy can be useful.