ABSTRACT
Organic salts of bismuth are currently used as antimicrobial agents against Helicobacter pylori. This study evaluated the antibacterial effect of elemental bismuth nanoparticles (Bi NPs) using a serial agar dilution method for the first time against different clinical isolates and a standard strain of H. pylori. The Bi NPs were biologically prepared and purified by a recently described method and subjected to further characterization by infrared spectroscopy and anti-H. pylori evaluation. Infrared spectroscopy results showed the presence of carboxyl functional groups on the surface of biogenic Bi NPs. These biogenic nanoparticles showed good antibacterial activity against all tested H. pylori strains. The resulting MICs varied between 60 and 100 µg/ml for clinical isolates of H. pylori and H. pylori (ATCC 26695). The antibacterial effect of bismuth ions was also tested against all test strains. The antimicrobial effect of Bi ions was lower than antimicrobial effect of bismuth in the form of elemental NPs. The effect of Bi NPs on metabolomic footprinting of H. pylori was further evaluated by (1)H NMR spectroscopy. Exposure of H. pylori to an inhibitory concentration of Bi NPs (100 µg/ml) led to release of some metabolites such as acetate, formic acid, glutamate, valine, glycine, and uracil from bacteria into their supernatant. These findings confirm that these nanoparticles interfere with Krebs cycle, nucleotide, and amino acid metabolism and shows anti-H. pylori activity.
Subject(s)
Anti-Bacterial Agents/pharmacology , Bismuth/pharmacology , Carboxylic Acids/chemistry , Helicobacter pylori/drug effects , Metabolomics/methods , Nanoparticles/chemistry , Magnetic Resonance Spectroscopy , Microbial Sensitivity Tests , Nanoparticles/ultrastructure , Spectrophotometry, Infrared , UltrasonicsABSTRACT
Today, synthesis of nanoparticles (NPs) using micro-organisms has been receiving increasing attention. In this investigation, a bismuth-reducing bacterium was isolated from the Caspian Sea in Northern Iran and was used for intracellular biosynthesis of elemental bismuth NPs. This isolate was identified as non-pigmented Serratia marcescens using conventional identification assays and the 16s rDNA fragment amplification method and used to prepare bismuth NPs. The biogenic bismuth NPs were released by liquid nitrogen and highly purified using an n-octanol water two-phase extraction system. Different characterisations of the purified NPs such as particle shapes, size and purity were carried out with different instruments. The energy-dispersive X-ray and X-ray diffraction (XRD) patterns demonstrated that the purified NPs consisted of only bismuth and are amorphous. In addition, the transmission electron micrograph showed that the small NPs formed larger aggregated NPs around <150â nm. Although the chemical syntheses of elemental bismuth NPs have been reported in the literature, the biological synthesis of elemental bismuth NPs has not been published yet. This is the first report to demonstrate a biological method for synthesising bismuth NPs and their purification with a simple solvent partitioning method.
Subject(s)
Bismuth/chemistry , Metal Nanoparticles/chemistry , Serratia marcescens/metabolism , Fresh Water/chemistry , Liquid-Liquid Extraction/methods , Metal Nanoparticles/ultrastructure , Microscopy, Electron, Scanning , Microscopy, Electron, Transmission , Particle Size , Seawater/chemistry , Serratia marcescens/isolation & purification , X-Ray DiffractionABSTRACT
BACKGROUND: In contrast to the homogeneously distributed deformation properties within the left ventricle, the right ventricular (RV) free wall (RVFW) shows a more inhomogeneous distribution. It has been demonstrated that pulmonary hypertension (PH) results in significant RVFW mechanical delay. OBJECTIVE: To assess the effect of the degree of pulmonary arterial systolic pressure on the RVFW strain gradient and on myocardial velocity generation. METHODS: Peak longitudinal strain and velocity data were collected from three different segments (basal, mid- and apical) of the RVFW in 17 normal individuals and 31 PH patients. RESULTS: A total of 144 RV wall segments were analyzed. RVFW strain values in individuals without PH were higher in the mid and apical segments than in the basal segment. In contrast, RVFW strain in PH patients was higher in basal segments and diminished toward the apex. In terms of RVFW velocities, both groups showed decremental values from basal to apical segments. Basal and mid-RVFW velocities were significantly lower in PH patients than in individuals without PH. CONCLUSIONS: PH results in significant alterations of strain and velocity generation that occurs along the RVFW. Of these abnormalities, the reduction in strain from the mid and apical RVFW segments was most predictive of PH. It is important to be aware of these differences in strain generation when studying the effect of PH on the right ventricle. Additional studies are required to determine whether these differences are due to RV remodelling.
Subject(s)
Echocardiography, Doppler/methods , Heart Ventricles/physiopathology , Hypertension, Pulmonary/physiopathology , Adult , Aged , Elasticity Imaging Techniques , Female , Humans , Hypertension, Pulmonary/diagnostic imaging , Male , Middle Aged , Myocardial Infarction/diagnostic imaging , Myocardial Infarction/physiopathology , ROC Curve , Stress, Mechanical , Ventricular Dysfunction, Right/diagnostic imaging , Ventricular Dysfunction, Right/physiopathology , Ventricular RemodelingABSTRACT
BACKGROUND: Right ventricular (RV) fractional area change and tricuspid annular plane systolic excursion (TAPSE) are recognised methods for assessing RV function. However, the way in which these variables are affected by varying degrees of pulmonary hypertension (PH) has not been well characterised. METHODS: RV end-systolic area (RVESA), RV end-diastolic area (RVEDA), pulmonary artery systolic pressure (PASP) and TAPSE were collected from a database of 190 patients who had been referred to the PH clinic for evaluation. RESULTS: The mean (SD) age of the study population was 56 (17) years; 82 men were included with a mean (SD) PASP of 54 (33) mm Hg (range 16-150), RVESA of 14 (9) cm(2), RVEDA of 24 (9) cm(2), RV fractional area change of 44 (18)% and TAPSE of 2.06 (0.69) cm. Receiver-operating characteristic curves identified TAPSE <2.01 cm, RV fractional area change <40.9%, RVESA >12.3 cm(2) and RVEDA >23.4 cm(2) as abnormal values with PH. Finally stratification of patients into sub-groups according to their PASP allowed means and standard deviations to be reported for each echocardiographic variable. CONCLUSION: This analysis provides a range of normal variables of RV size and function, not previously published, that can be used in routine evaluation and follow-up of patients with PH.
Subject(s)
Heart Ventricles/diagnostic imaging , Hypertension, Pulmonary/physiopathology , Ventricular Function, Right/physiology , Echocardiography, Doppler , Female , Heart Ventricles/physiopathology , Humans , Hypertension, Pulmonary/diagnostic imaging , Male , Middle Aged , Severity of Illness IndexABSTRACT
OBJECTIVES: The relationship between the changes of weight (WT) and electrocardiogram (ECG) QRS amplitude in patients with anasarca (AN) was evaluated. BACKGROUND: Attenuation of the ECG voltage occurs as the electrical current spreads from the epicardium to the body surface. The voltage registered is a function of the cardiac potentials, the electrical resistivities of the intervening tissues and the orientation of the ECG leads with respect to the direction of propagation of excitation. Lung congestion and pericardial and pleural effusions can cause attenuation in the ECG potentials; additionally, a similar change was recently observed in patients with AN. METHODS: A prospective study of this phenomenon in 28 patients with a critical illness was carried out. Electrocardiograms and patients' WTs were recorded daily. Pericardial effusions were excluded by serial echocardiograms. The sums of the amplitude of QRS complexes from the 12 ECG leads (Sigma QRS) were correlated with the corresponding WTs. Intracardiac ECGs, done in three patients, were correlated with surface ECGs. RESULTS: Admission WT was 148.9 +/- 37.8 lbs, and it peaked to 197.8 +/- 52.3 lbs (p = 0.0005). Admission Sigma QRS was 120.2 +/- 41.6 mm and dropped to 54.8 +/- 26.9 mm at time of peak WT (p = 0.0005). Regression of Sigma QRS on WT revealed an r = 0.61 and a p = 0.0005. Subsequent WT loss in 13 patients (from 219.0 +/- 40.7 lbs to 179.5 +/- 41.7 lbs, p = 0.001) led to an increase of Sigma QRS from 53.5 +/- 24.5 mm to 86.8 +/- 38.2 mm (p = 0.001). Intracardiac ECGs remained stable, while surface ECGs changed with perturbations of WT. CONCLUSIONS: Attenuation of ECG voltage in patients with AN correlates with WT gain, and it can be attributed to a shunting of the cardiac potentials due to the low resistance of the AN fluid.
Subject(s)
Body Weight , Edema/physiopathology , Electrocardiography , Heart Conduction System/physiopathology , Adult , Aged , Aged, 80 and over , Critical Illness , Female , Humans , Male , Middle Aged , Prospective StudiesABSTRACT
In resting platelets integrin alphaIIbbeta3 is constitutively expressed in an inactive state and it does not recognize soluble proteins. Platelet activation results in a conformational change of the low-affinity alphaIIbbeta3 to a high-affinity state which then recognizes plasma fibrinogen. The ectopic expression of alphaIIbbeta3 integrin in rodent and human cells derived from solid tumors is well documented, although little is known about its affinity state in these tumor cells. In this study we analysed expression and function of high-affinity alphaIIbbeta3 in murine metastatic melanoma B16a cells by using a mAb that specifically recognizes high-affinity alphaIIbbeta3 (PAC-1). These tumor cells while in suspension bound PAC-1 and fibrinogen. Immunofluorescent studies of B16a cells indicated that high-affinity alphaIIbbeta3 is associated with the Golgi complex and the cell surface. Stimulation of B16a cells with a PKC-activator, 12(S)-HETE, induced translocation of the high-affinity integrin from an intracellular pool to the plasma membrane, which resulted in increased tumor cell adhesion to fibronectin. In addition to participating in 12(S)-HETE-stimulated adhesion of B16a cells, the high-affinity alphaIIbbeta3 integrin is also involved in tumor cell invasion through a reconstituted basement membrane. In conclusion, results from this study suggest that in non-megakaryocytic lineage B16a cells alphaIIbbeta3 is constitutively expressed in a high-affinity state, and that this conformation participates in tumor cell adhesion and invasion.
Subject(s)
Melanoma, Experimental/metabolism , Platelet Glycoprotein GPIIb-IIIa Complex/biosynthesis , Animals , Cell Adhesion/drug effects , Dual Specificity Phosphatase 2 , Fibrinogen/metabolism , Humans , Melanoma, Experimental/pathology , Melanoma, Experimental/secondary , Mice , Neoplasm Invasiveness , Platelet Glycoprotein GPIIb-IIIa Complex/physiology , Protein Phosphatase 2 , Protein Tyrosine Phosphatases/metabolismABSTRACT
The integrin alphaIIb beta3 is a membrane receptor which was considered to be expressed only in cells of megakaryocytic lineage. We have shown that alphaIIb beta3 is expressed in mouse melanoma B16a cells, and in human prostate adenocarcinoma cells. The purpose of this study was to determine whether the megakaryocytic product alphaIIb beta3 was functionally expressed in other non-megakaryocyte lineage tumor cells. By using the reverse transcription polymerase chain reaction (RT-PCR), we have obtained data demonstrating that alphaIIb beta3 is expressed in a variety of tumor cell lines (17) derived from different species (human, rat and mouse) and of different histological origins (skin, blood, lung, liver, kidney, cervix, colon, bladder, breast and prostate). Immunostaining of tumor cells with a monoclonal antibody (MAb) to alphaIIb beta3 demonstrates that alphaIIb beta3 protein is also expressed in tumor cells. A protein kinase C activator PMA stimulates adhesion of tumor cells to fibronectin and fibrinogen, and this stimulated adhesion is blocked by a function-blocking MAb directed to alphaIIb beta3. Our results indicate that the megakaryocytic gene product alphaIIb beta3 integrin is widely expressed among tumor cells of non-megakaryocytic lineage, suggesting that ectopic expression of this integrin may play an important role in tumor progression.
Subject(s)
Blood Platelets/metabolism , Neoplasms/metabolism , Platelet Glycoprotein GPIIb-IIIa Complex/biosynthesis , Adenocarcinoma/metabolism , Adenocarcinoma/pathology , Amino Acid Sequence , Animals , Base Sequence , Cell Adhesion/physiology , Humans , Integrins/biosynthesis , Male , Melanoma, Experimental/metabolism , Melanoma, Experimental/pathology , Mice , Molecular Sequence Data , Neoplasms/pathology , Platelet Glycoprotein GPIIb-IIIa Complex/metabolism , Platelet Glycoprotein GPIIb-IIIa Complex/physiology , Polymerase Chain Reaction , Prostatic Neoplasms/metabolism , Prostatic Neoplasms/pathology , RNA, Messenger/metabolism , Rats , Sequence Homology, Amino Acid , Sequence Homology, Nucleic Acid , Species Specificity , Transcription, Genetic , Tumor Cells, CulturedSubject(s)
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid/pharmacology , Cytoskeleton/pathology , Integrins/biosynthesis , Platelet Glycoprotein GPIIb-IIIa Complex/biosynthesis , Protein Processing, Post-Translational/drug effects , Animals , Carcinosarcoma , Cell Adhesion , Cell Membrane/drug effects , Cell Membrane/physiology , Cytoskeleton/drug effects , Cytoskeleton/ultrastructure , Gene Expression Regulation, Neoplastic/drug effects , Lung Neoplasms , Melanoma, Experimental , Mice , Nocodazole/pharmacology , Organelles/drug effects , Organelles/metabolism , Organelles/pathology , Rats , Tumor Cells, CulturedABSTRACT
The binding of autocrine motility factor (AMF) to its cell surface receptor, gp78, stimulates tumor cell motility. In this report, we provide evidence that stimulation of gp78 by either AMF or a monoclonal antibody to gp78 (3F3A) increases adhesion and spreading of metastatic murine melanoma (B16a) cells on fibronectin. This gp78-regulated increase is mediated by up-regulation of surface alphaIIbbeta3++ and alpha5beta1 integrin receptors. In addition, AMF treatment of B16a cells increased translocation of alphaIIbbeta3 and alpha5beta1 from the cytoplasm to the cell surface. However, alphaIIbbeta3 and alpha5beta1 demonstrate separate and unique staining patterns at the surface of B16a cells in response to stimulation of gp78. Furthermore, stimulation of B16a cells with AMF increased their invasion through Matrigel. This stimulated invasion was inhibited by antibodies to alphaIIbbeta3 but not by antibodies to alpha5beta1. The increased integrin surface expression and function in response to AMF was blocked by N-benzyl-N-hydroxy-5-phenylpentanamide, an inhibitor of 12-lipoxygenase, and calphostin C, an inhibitor of protein kinase C. The results demonstrate that AMF stimulates integrin-mediated B16a cell adhesion, spreading, and invasion, and these events are regulated by a signaling pathway involving 12-lipoxygenases and protein kinase C.
Subject(s)
Cell Adhesion , Glucose-6-Phosphate Isomerase/pharmacology , Melanoma, Experimental/pathology , Melanoma, Experimental/physiopathology , Neoplasm Metastasis , Animals , Cell Adhesion/drug effects , Cell Line , Cell Membrane/physiology , Collagen , Culture Media, Conditioned , Cyclooxygenase Inhibitors/pharmacology , Drug Combinations , Fibronectins , Fibrosarcoma , Flow Cytometry , Glucose-6-Phosphate Isomerase/isolation & purification , Humans , Laminin , Lipoxygenase Inhibitors/pharmacology , Mice , Models, Biological , Neoplasm Invasiveness , Platelet Glycoprotein GPIIb-IIIa Complex/biosynthesis , Proteoglycans , Receptors, Fibronectin/biosynthesis , Signal Transduction , Tumor Cells, CulturedABSTRACT
In B16a melanoma cells, protein kinase-C-alpha (PKC alpha) is immunomorphologically associated with cytoplasmic vesicles in addition to the previously observed locations (plasma membrane, cytoskeleton, nucleus), as detected with monoclonal antibody (MAb) MC3a. Subcellular fractionation indicated that the authentic 80-KD protein as well as PKC activity can be detected in several particulate fractions except for L2, which contains dense lysosomes. The highest PKC activity is associated with the cytosol-ultralight vesicles and the L1 fraction (containing plasma membrane, endosomes, and the Golgi apparatus). Both of these fractions contained the fluid-phase endocytosis marker peroxidase, indicating that PKC alpha, in addition to other subcellular structures, is most probably associated with endosomal membranes in B16a melanoma cells.
Subject(s)
Isoenzymes/metabolism , Melanoma/enzymology , Protein Kinase C/metabolism , Animals , Cytoplasm/enzymology , Immunohistochemistry , Melanoma/pathology , Mice , Protein Kinase C-alpha , Subcellular Fractions , Tumor Cells, CulturedABSTRACT
Previous work demonstrated that 12(S)-HETE [12(S)-hydroxyeicosatetraenic acid], a lipoxygenase metabolite of arachidonic acid, stimulates the surface expression of integrin alpha v beta 3 on mouse lung vascular endothelial cells (CD clone 3) in a post-transcriptional and protein kinase C (PKC)-dependent fashion. In this study we examined the effect of 12(S)-HETE on the expression of integrin receptors alpha v beta 3 and alpha 5 beta 1 in a different clone of a mouse endothelial cell population derived from lung microvasculature (designated CD clone 4). The results indicated that 12(S)-HETE transcriptionally activates the gene expression of integrin alpha v as assessed by quantitative reverse transcription/polymerase chain reaction/Southern hybridization, RNase protection assay, solution hybridization, and northern blotting. The induction of alpha v mRNA occurred within 1 hour, peaked at approximately 4 hours (2- to 4-fold increase), persisted for up to 16 hours, and thereafter gradually declined. The PKC activator phorbol 12-myristate 13-acetate (PMA) induced the alpha v mRNA, in a similar way. 12(S)-HETE treatment did not, in contrast, alter the mRNA levels of integrin subunit alpha 5 or beta 1. The induction of alpha v mRNA appeared to be protein synthesis-independent, since cycloheximide did not alter the 12(S)-HETE effect. 12(S)-HETE also did not appear to alter the mRNA half-life of alpha v. On the other hand, 12(S)-HETE-induced increase in alpha v mRNA levels was PKC-dependent, since pretreatment of CD clone 4 cells with calphostin C significantly inhibited 12(S)-HETE-increased alpha v mRNA. Nuclear runoff experiments revealed that the increase in alpha v mRNA results from an enhanced gene transcription. Facilitated alpha v gene transcription resulted in an increased surface expression of alpha v beta 3 protein, which resulted in an increased cell adhesion to vitronectin. The above observations, in conjunction with our previous experimental data, suggest that 12(S)-HETE may employ diverse mechanisms to stimulate the integrin alpha v beta 3 expression in vascular endothelial cells, which could play important roles in tumor cell adhesion, angiogenesis, hemostasis, and many other vascular events.
Subject(s)
Antigens, CD/biosynthesis , Endothelium, Vascular/metabolism , Hydroxyeicosatetraenoic Acids/pharmacology , Protein Kinase C/metabolism , Transcriptional Activation/drug effects , 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid , Animals , Antibodies , Antigens, CD/analysis , Base Sequence , Blotting, Northern , Blotting, Southern , Cell Nucleus/metabolism , Clone Cells , DNA Primers , Endothelium, Vascular/cytology , Endothelium, Vascular/drug effects , Flow Cytometry , Fluorescent Antibody Technique , Integrin alphaV , Mice , Molecular Sequence Data , Polymerase Chain Reaction , Pulmonary Circulation , RNA, Messenger/analysis , RNA, Messenger/biosynthesis , Tetradecanoylphorbol Acetate/pharmacologyABSTRACT
In metastatic B16a murine melanoma cells, alpha IIb beta 3 integrin was shown to be one of the key adhesion molecules responsible for matrix adhesion and spreading. Upon stimulation, alpha IIb beta 3 can be upregulated at the cell surface due to translocation of the receptor to the plasma membrane from an intracellular pool. Here we have characterized this integrin pool as a tubulovesicular structure (TVS) corresponding to endosomes. TVS was found to be associated temporarily with microtubules and intermediate filaments especially after protein kinase C (PKC) stimulation with a lipoxygenase metabolite of arachidonic acid, 12-(S)-hydroxyeicosatetraenoic acid [12-(S)-HETE]. After PKC stimulation, the predominantly vesicular TVS became elongated and alpha IIb beta 3 appeared at the apical plasma membrane and microvilli. Disruption of either the microtubules or intermediate filaments prevented the 12-(S)-HETE effect both on vesicular to tubular transition of TVS as well as on surface expression of this integrin. The connection with the Golgi system of the integrin-containing TVS was proved by a Golgi-inhibitor (brefeldin A) pretreatment, which prevented the PKC-stimulation-induced TVS elongation and subsequent receptor-upregulation at the cell surface. After a soluble ligand binding (mAb to the alpha IIb beta 3 complex) the surface receptor endocytosed back to the TVS indicating the presence of a dynamic, cytoskeleton associated integrin pool in melanoma cells.
Subject(s)
Hydroxyeicosatetraenoic Acids/pharmacology , Integrins/metabolism , 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid , Animals , Cytoskeleton/physiology , Endosomes/ultrastructure , Fluorescent Antibody Technique , Integrins/ultrastructure , Melanoma, Experimental , Mice , Microscopy, Electron , Platelet Glycoprotein GPIIb-IIIa Complex , Tumor Cells, CulturedABSTRACT
The process of tumor cell invasion of the basement membrane is proposed to consist of three steps: attachment, local proteolysis and migration. 12-(S)-HETE, a 12-lipoxygenase metabolite of arachidonic acid, upregulates surface expression of integrin cytoadhesins and an autocrine motility factor receptor, suggesting that this metabolite may play an important regulatory function in tumor cell invasion. In the present study, we determined whether 12-(S)-HETE affects surface expression and/or release of cathepsin B, a cysteine protease that has been implicated in focal degradation of basement membrane. Secretion and distribution of cathepsin B was evaluated in two model systems for various stages of neoplastic progression: (i) murine B16 melanoma lines of low (B16-F1) and high (B16a) lung colonization potential, and (ii) immortalized and ras-transfected MCF-10 human breast epithelial cells that differ in their invasive capacities in vitro. In the B16a cells, 12-(S)-HETE induced release of native and latent cathepsin B activity and concomitantly reduced cell-associated cathepsin B immunoreactivity. In contrast, 12-(S)-HETE did not induce the release of cathepsin B from B16-F1 cells, suggesting that there may be an enhanced response to 12-(S)-HETE in more malignant cells. This was confirmed in the MCF-10 system, in which 12-(S)-HETE was able to induce the release of cathepsin B from the ras-transfected cells, but not from the immortal cells. A simultaneous reduction in staining for cathepsin B was observed in the ras-transfected cells, but not in their immortal counterparts. The release of cathepsin B may be mediated by PKC as pretreatment of B16a cells with the selective PKC inhibitor calphostin C, but not with the PKA inhibitor H8, prevented the stimulated release of cathepsin B. In B16a cells, the release of cathepsin B was accompanied by a translocation toward the cell periphery of vesicles staining for cathepsin B, resulting in focal areas of accumulation of cathepsin B. After 12-(S)-HETE stimulation of the ras-transfected MCF-10 cells, cathepsin B was distributed homogeneously on the apical surface. Thus, 12-(S)-HETE can upregulate the surface expression on tumor cells of proteins able to mediate each of the three steps of tumor cell invasion: adhesion, degradation, and migration.
Subject(s)
Breast Neoplasms/metabolism , Cathepsins/metabolism , Hydroxyeicosatetraenoic Acids/pharmacology , Melanoma, Experimental/metabolism , Naphthalenes , Protein Kinase C/metabolism , 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid , Animals , Biological Transport , Cathepsins/immunology , Cell Compartmentation , Enzyme Activation , Fluorescent Antibody Technique , Humans , Integrins/biosynthesis , Lipoxygenase/metabolism , Mice , Neoplasm Metastasis , Polycyclic Compounds/pharmacology , Protein Kinase C/antagonists & inhibitors , Stereoisomerism , Tumor Cells, CulturedABSTRACT
Cellular motility, a prerequisite for metastasis of tumor cells, is affected by a 55-kDa tumor-cell-secreted cytokine which influences the migration of the producing cells and is called autocrine motility factor (AMF). Previous studies indicated that AMF stimulates motility by binding to its receptor, a cell-surface glycoprotein of 78 kDa (gp78), inducing its phosphorylation, activating a pertussis toxin (PT)-sensitive G-protein, and stimulating inositol metabolism. However, the intracellular signaling mechanisms which transduce and regulate the AMF motility response remain largely unknown. 12-(S)-HETE, a lipoxygenase metabolite of arachidonic acid which affects the cytoskeletal architecture of murine melanoma cells, also stimulates cell motility independently of PT-sensitive G-proteins and up-regulates gp78 surface expression. 12-(S)-HETE induces the phosphorylation of gp78 in a manner analogous to AMF and the motility response of these murine melanoma cells to both AMF and 12-(S)-HETE is inhibited by protein kinase C inhibitors. Furthermore, perturbation of the AMF receptor stimulated endogenous biosynthesis of 12(S)HETE. These results suggest the existence of an "autocrine motility cycle" which influences melanoma cell motility by gp78 activation, and production of second messengers which affect the cytoskeletal architecture and expression of the AMF receptor itself.
Subject(s)
Cell Movement/drug effects , Glucose-6-Phosphate Isomerase/pharmacology , Hydroxyeicosatetraenoic Acids/pharmacology , Melanoma, Experimental/pathology , Membrane Glycoproteins/metabolism , Animals , Melanoma, Experimental/metabolism , Mice , Pertussis Toxin , Phosphorylation , Protein Kinase C/metabolism , Up-Regulation/drug effects , Virulence Factors, Bordetella/pharmacologyABSTRACT
The fatty acid 12(S)-HETE may be a new second messenger capable of activating PKC. In tumor cells 12(S)-HETE stimulates cytoskeleton-dependent cellular responses such as adhesion and spreading. Analysis of 12(S)-HETE effects on B16a melanoma cell cytoskeleton revealed reversible rearrangement of microtubules, microfilaments, the actin-binding proteins, vinculin, myosin heavy (MHC) and light chains (MLC), as well as bundling of vimentin intermediate filaments. The alterations in microfilaments and intermediate filaments occurred very rapidly, i.e., 5 min after exposure of tumor cells to 12(S)-HETE. The 12(S)-HETE-induced cytoskeletal alterations were accompanied by centrifugal organelle-translocation. Interestingly, MLC exhibited clear association with the cytoplasmic organelles. Biochemical analysis of the 12(S)-HETE effect indicated a PKC-mediated reversible hyperphosphorylation of MLC, vimentin, and a 130 kD cytoskeletal-associated protein. Optimal effects were obtained after 5 min treatment with 12(S)-HETE at 0.1 microM concentration. 12(S)-HETE pretreatment induced tumor cell spreading on a fibronectin matrix which required the intactness of all three major cytoskeletal components. The spreading process was dependent upon the activity of PKC. Our data suggest that 12(S)-HETE is a physiological stimulant of PKC. Further, it induces rearrangement of the cytoskeleton of tumor cells in interphase resulting in the stimulation of cytoskeleton-dependent cell activity such as spreading.
Subject(s)
Calcium/pharmacology , Cytoskeleton/ultrastructure , Hydroxyeicosatetraenoic Acids/pharmacology , Melanoma, Experimental/pathology , Phospholipids/pharmacology , Protein Kinases/pharmacology , Skin Neoplasms/pathology , 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid , Actin Cytoskeleton/chemistry , Actin Cytoskeleton/drug effects , Actin Cytoskeleton/ultrastructure , Actins/analysis , Animals , Cell Movement/physiology , Cytoskeletal Proteins/metabolism , Cytoskeletal Proteins/physiology , Cytoskeleton/chemistry , Cytoskeleton/drug effects , Dose-Response Relationship, Drug , Fibronectins/analysis , Fluorescent Antibody Technique , Immunohistochemistry , Intermediate Filaments/chemistry , Intermediate Filaments/drug effects , Intermediate Filaments/ultrastructure , Melanoma, Experimental/chemistry , Melanoma, Experimental/ultrastructure , Mice , Myosins/analysis , Organelles/physiology , Organelles/ultrastructure , Phosphorylation , Protein Kinases/physiology , Skin Neoplasms/chemistry , Skin Neoplasms/ultrastructure , Tumor Cells, Cultured , Vimentin/analysis , Vinculin/analysisABSTRACT
Tumor cell interaction with the endothelium of the vessel wall is a rate limiting step in metastasis. The fatty acid modulation of this interaction was investigated in low (LM) and high (HM) metastatic B16 amelanotic melanoma (B16a) cells. 12(S)-HETE increased the adhesion of LM cells to endothelium derived from pulmonary microvessels. All other monohydroxy and dihydroxy fatty acids were ineffective. LTB4 induced a modest stimulation but LTC4, LTD4, LTE4 as well as LXA4 and LXB4 were ineffective. The 12(S)-HETE enhanced adhesion of B16a cells was inhibited by pretreatment with 13(S)-HODE but not by 13(R)-, 9(S)-HODE or 13-OXO-ODE. 13(S)-HODE decreased adhesion of HM B16a cells to endothelium. 12(S)-HETE enhanced surface expression of integrin alpha IIb beta 3 and monoclonal antibodies against this integrin but not against alpha 5 beta 1, blocked enhanced but not basal adhesion to endothelium. Intravenous injection of 12(S)-HETE treated LM cells resulted in increased lung colonization (experimental metastasis). This effect was specific for 12(S)-HETE and was inhibited by 13(S)-HODE but not by other HODE's. 12(S)-HETE also enhanced lung colonization by HM cells and 13(S)-HODE decreased lung colonization by HM cells. Our results suggest a highly specific bidirectional modulation of metastatic phenotype and lung colonization by 12(S)-HETE and 13(S)-HODE.
Subject(s)
Eicosanoids/physiology , Endothelium, Vascular/cytology , Melanoma, Experimental/pathology , Neoplasm Metastasis , Animals , Cell Adhesion , Integrins/metabolism , Lung Neoplasms/secondary , Male , Mice , Mice, Inbred C57BL , Tumor Cells, CulturedABSTRACT
Tumor-cell interaction with the vessel wall during metastasis involves adhesion, induction of endothelial-cell retraction and spreading on the exposed sub-endothelial matrix. The signals for initiation of tumor-cell spreading and the receptors involved are unknown. A protocol was developed to distinguish between initial tumor-cell (B16 amelanotic melanoma; B16a) adhesion to and spreading on fibronectin. The time for maximum spreading was 50 min. Treatment with a lipoxygenase metabolite of arachidonic acid [12(S)-HETE] resulted in maximum spreading in 15 min (max. effect approx. 0.1 microM). Other lipoxygenase metabolites were ineffective. 12(S)-HETE treatment induced a rearrangement of F-actin, vinculin, vimentin intermediate filaments and integrin alpha IIb beta 3, but not integrin alpha 5 beta 1. Antibodies to alpha IIb beta 3 but not alpha 5 beta 1 blocked the 12(S)-HETE effect on B16a spreading. B16a-cell attachment to fibronectin resulted in increased metabolism of arachidonic acid to 12(S)-HETE, which was inhibited by lipoxygenase but not by cyclo-oxygenase inhibitors. Accordingly, lipoxygenase inhibitors but not cyclo-oxygenase inhibitors blocked spontaneous B16a-cell spreading. The protein-kinase-C inhibitors calphostin C, H7 and staurosporine also inhibited spreading, while the protein-kinase-A inhibitor H8 was ineffective. These data suggest that B16a-cell spreading on fibronectin is initiated by a lipoxygenase metabolite [12(S)-HETE] of arachidonic acid and is mediated by protein kinase C.
Subject(s)
Fibronectins/physiology , Hydroxyeicosatetraenoic Acids/pharmacology , Integrins/physiology , 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid , Animals , Arachidonic Acid/metabolism , Cell Adhesion/drug effects , Cytoskeleton/chemistry , Cytoskeleton/drug effects , Hydroxyeicosatetraenoic Acids/biosynthesis , Integrins/analysis , Mice , Mice, Inbred C57BL , Protein Kinases/physiologyABSTRACT
We recently reported that the Ca(2+)- and phospholipid-dependent protein kinase, protein kinase C (PKC), was involved in rat Walker carcinosarcoma cell adhesion to large-vessel endothelium. We extended our studies to explore the role of this kinase in the adhesion to small-vessel endothelium and lung colonization of murine B16 amelanotic melanoma (B16a). Subpopulations of B16a cells, which differ in lung-colonization potentials, were isolated by centrifugal elutriation from solid tumors. In this study, we demonstrate that cells from a high metastatic sub-population (HM340), when compared with cells from a low metastatic sub-population (LM180), exhibit elevated levels of total cellular as well as membrane-bound PKC. The increase in PKC in cells from the HM340 correlates positively to their increased ability to adhere to murine pulmonary-microvessel endothelial-cell monolayer, and to form pulmonary colonies in syngeneic mice. Calphostin C, a potent and selective PKC inhibitor, decreases in a dose-dependent manner the adhesion to endothelium and the lung colonization of cells from both the low and the high metastatic sub-populations with IC50 at sub-micromolar concentrations. In conclusion, our results suggest that PKC may be a key element in regulating tumor-cell metastasis and that PKC inhibitors may be anti-metastatic agents.
Subject(s)
Endothelium, Vascular/physiology , Lung Neoplasms/secondary , Melanoma, Experimental/pathology , Naphthalenes , Polycyclic Compounds/pharmacology , Protein Kinase C/antagonists & inhibitors , 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid , Animals , Cell Adhesion , Hydroxyeicosatetraenoic Acids/analysis , Mice , Protein Kinase C/analysisSubject(s)
Urethra/diagnostic imaging , Urinary Bladder/diagnostic imaging , Urinary Incontinence/diagnostic imaging , Child , Child, Preschool , Diatrizoate Meglumine , Female , Humans , Infant , Infant, Newborn , Iothalamate Meglumine , Male , Methods , Radiography , Urinary Catheterization , UrinationABSTRACT
Over a period of 3 years, 68 intrahepatic abscesses in 48 patients were detected by ultrasonography at our institution in New Delhi, India. Our experiences with the sonography and management of this entity are presented. Multiple abscesses were present in 14 patients; the rest had a single abscess. The most common location was the right lobe of the liver. The sonographic morphology of the abscess was analyzed in terms of the echo pattern of the wall and contents of the abscess. These were later correlated with the duration of illness. An attempt was made to assess healing and thereby the age of the abscess. Follow-up ultrasound scans indicated that the change in sonographic appearance lags behind clinical improvement by a considerable period of time.