Efficacy of ULV and thermal aerosols of deltamethrin for control of Aedes albopictus in nice, France.

BACKGROUND: Ultra-low volume (ULV) insecticidal aerosols dispensed from vehicle-mounted cold-foggers are widely considered the method of choice for control of Aedes aegypti and Ae. albopictus during outbreaks of dengue and chikungunya and, more recently, Zika. Nevertheless, their effectiveness has been poorly studied, particularly in Europe. Nearly all published studies of ULV efficacy are bio-assays based on the mortality of caged mosquitoes. In our study we preferred to monitor the direct impact of treatments on the wild mosquito populations. This study was undertaken to evaluate the efficiency of the two widely used space spraying methods to control Ae. albopictus and Ae. aegypti. METHODS: We determined the susceptibility of local Ae. albopictus to deltamethrin by two methods: topical application and the "WHO Tube Test". We used ovitraps baited with hay infusion and adult traps (B-G Sentinel) baited with a patented attractant to monitor the mosquitoes in four residential areas in Nice, southern France. The impact of deltamethrin applied from vehicle-mounted ULV fogging-machines was assessed by comparing trap results in treated vs untreated areas for 5 days before and 5 days after treatment. Four trials were conducted at the maximum permitted application rate (1 g.ha-1). We also made two small-scale tests of the impact of the same insecticide dispensed from a hand-held thermal fogger. RESULTS: Susceptibility to the insecticide was high but there was no discernable change in the oviposition rate or the catch of adult female mosquitoes, nor was there any change in the parous rate. In contrast, hand-held thermal foggers were highly effective, with more than 90% reduction of both laid eggs and females. CONCLUSIONS: We believe that direct monitoring of the wild mosquito populations gives a realistic assessment of the impact of treatments and suggest that the lack of efficacy is due to lack of interaction between the target mosquitoes and the ULV aerosol. We discuss the factors that influence the effectiveness of both methods of spraying in the context of epidemic situations.

A rainfall- and temperature-driven abundance model for Aedes albopictus populations.

The mosquito Aedes (Stegomyia) albopictus (Skuse) (Diptera: Culicidae) is an invasive species which has colonized Southern Europe in the last two decades. As it is a competent vector for several arboviruses, its spread is of increasing public health concern, and there is a need for appropriate monitoring tools. In this paper, we have developed a modelling approach to predict mosquito abundance over time, and identify the main determinants of mosquito population dynamics. The model is temperature- and rainfall-driven, takes into account egg diapause during unfavourable periods, and was used to model the population dynamics of Ae. albopictus in the French Riviera since 2008. Entomological collections of egg stage from six locations in Nice conurbation were used for model validation. We performed a sensitivity analysis to identify the key parameters of the mosquito population dynamics. Results showed that the model correctly predicted entomological field data (Pearson r correlation coefficient values range from 0.73 to 0.93). The model's main control points were related to adult's mortality rates, the carrying capacity in pupae of the environment, and the beginning of the unfavourable period. The proposed model can be efficiently used as a tool to predict Ae. albopictus population dynamics, and to assess the efficiency of different control strategies.