Reproductive toxicity of roundup®-treated feed on broiler breeder roosters and the amelioration of these deleterious effects with inclusion of humic acids in feed.

Glyphosate-based herbicides (GBHs) such as RoundUp® are a staple of modern crop production, and as a result, residues of their ingredients are typically found in animal feeds. GBH ingredients have repeatedly been shown to impact the male reproductive health of various animals, but at present, the impact of GBH exposures on reproductive health have not been investigated in broiler breeder roosters. This study sought to determine the effect of these exposures on roosters as well as the ability of humic acids (HA) to counteract the effect of GBH exposure. Through 18 weeks of treatment with Roundup®-added or HA-added feeds compared against a common broiler breeder mash, negative effects of Roundup® exposure were seen on testis morphology as well as sperm quality. Increased exposure to Roundup® ingredients resulted in increased vacuolation of seminiferous tubule epithelium. Exposure to Roundup® impacted assessments of sperm quality including sperm mobility, viability and count during the experimental trail. HA supplementation served as a promising adsorptive additive by improving both morphology and sperm quality during the trial. Roundup® exposure was shown to have a negative influence on broiler breeder rooster reproductive health while HA improved reproductive health. The use of HA as an absorbent additive in broiler breeder feeds shows promise in improving reproductive efficiency in broiler breeders.

Effects of coenzyme Q10 on reproductive performance of laying Japanese quail (Coturnix japonica) under cadmium challenge.

Japanese quail is an increasingly important bird of economic importance for commercial egg and meat production, particularly in developing countries. There is a need for research aimed at improving efficiency of these birds during stressful challenges, such as oxidative stress. Coenzyme Q10 (CoQ10), a highly functional antioxidant, protects cells against oxidative stress. This study was conducted to determine the effects of CoQ10 on reproductive performance of Japanese quail under cadmium (Cd) challenge. A total of 216 six-wk-old Japanese quail were randomly allocated into 3 groups for an 8 wk experimental trial. The treatments include a negative control (NC): feeding basal diet; a positive control (PC): feeding basal diet and cadmium administration (1 mg/100 g BW, at 10 and 11 wk of age), and (CdQ10): feeding CoQ10 supplemented (900 mg/kg diet) basal diet and Cd administration. At 11 and 13 wk of age, egg production, body weight, mortality, oviduct, and ovarian biometry, were recorded. Histology and histopathology of isthmus and magnum, fertility, hatchability, hatchling quality, and HSP70 mRNA transcript abundance in the utero-vaginal junction (UVJ) were evaluated. Positive control and CdQ10 group had no significant effect on live body weight, stroma weight, follicle size, hatchability, and fertility; however, Cd administration increased (P < 0.01) mortality rate in the PC group compared to the NC and CdQ10 groups. CdQ10 quail produced more eggs and had a higher hatchling quality compared to the PC group (P < 0.01). The thickness and height of isthmus and magnum folds in the CdQ10 group was increased compared to the PC group (P < 0.01) and overall oviduct weight was increased with CoQ10 supplementation (P < 0.01). Compared to PC, the CdQ10 group had a reduction in infiltration of inflammatory cells. Relative abundance of HSP70 mRNA in UVJ was influenced by interactive effect of treatment × time (P < 0.05). In conclusion, dietary supplementation of CoQ10 showed beneficial effects on some reproduction characteristics of female Japanese quail under Cd-induced oxidative stress.

Glyphosate-based herbicide formulations and reproductive toxicity in animals.

The adoption of genetically engineered (GE) crops in agriculture has increased dramatically over the last few decades. Among the transgenic plants, those tolerant to the herbicide glyphosate are among the most common. Weed resistance to glyphosate-based herbicides (GBHs) has been on the rise, leading to increased herbicide applications. This, in turn, has led to increased glyphosate residues in feed. Although glyphosate has been considered to be generally safe to animal health, recent studies have shown that GBHs have potential to cause adverse effects in animal reproduction, including disruption of key regulatory enzymes in androgen synthesis, alteration of serum levels of estrogen and testosterone, damage to reproductive tissues and impairment of gametogenesis. This review emphasizes known effects of GBHs on reproductive health as well as the potential risk GBH residues pose to animal agriculture.

Sperm Collection of Differential Quality Using Density Gradient Centrifugation.

In sexual reproduction, a male gamete or sperm cell fuses with a female gamete to bring about fertilization. However, a large number of sperm cells with fertilizing ability are required to interact with a female gamete to ensure fertilization. As such, the fertilizing ability of individual sperm cells is critical for successful reproduction. Density gradient centrifugation has been utilized for several decades as a reproducible, fast, efficient, effective and extremely adaptable method to collect only high-quality sperm to be used in assisted reproductive technology. The protocols we described herein focus on the utilization of the discontinuous Percoll density gradient centrifugation (PDGC) technique to isolate three distinct populations of rooster sperm by their quality. We were able to collect low-, medium- and high-quality sperm. We also describe reproducible protocols that entail determining fertility potential of sperm by assessing their viability, mobility and penetrability. Collection of sperm by their quality using PDGC technique would be useful to accurately and thoroughly characterize sperm with differential fertility potential.

D-Aspartate amends reproductive performance of aged roosters by changing gene expression and testicular histology.

Male broiler breeders (n=32) of 55 weeks of age were administered four different doses of capsulated d-aspartate (DA; 0, 100, 200 or 300mgkg-1day-1, p.o. (DA0, DA100, DA200 and DA300 respectively)) for 12 successive weeks to assess reproductive performance, blood testosterone, testicular histology and transcript levels of steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme (P450scc), androgen receptor (AR), LH receptor (LHR), 3ß-hydroxysteroid dehydrogenase (3BHSD), proliferating cell nuclear antigen (PCNA), glutamate ionotropic receptor NMDA type subunit 1 (GRIN1) and glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B). Blood samples and ejaculates were collected, and bodyweight was recorded weekly for 10 weeks. AI was performed weekly for the last 2 weeks to determine the number of sperm penetration holes in the perivitelline layer, fertility and hatchability. Testes histology and transcript levels were evaluated in the 12th week. Bodyweight, numbers of Leydig cells and blood vessels, testis index and levels of sperm abnormalities were not affected (P>0.05) by the treatment. However, sperm total and forward motility, plasma membrane integrity and functionality of sperm, ejaculate volume, testosterone concentration and fertility were higher (P<0.05) in both the DA200 and DA300 groups compared with the other groups. In the DA100 and DA200 groups, sperm concentration, number of spermatogonia, thickness of the seminiferous epithelium and the diameter of tubules were significantly higher (P<0.05) than the other DA-treated groups. The number of penetration holes, hatchability and malondialdehyde concentration were higher in the DA200, all DA-treated and DA300 groups respectively compared with the control and other treatment groups. Except for P450scc, AR, LHR and PCNA transcript levels in the DA300 groups, the relative expression of the genes evaluated improved significantly in the other DA-treated groups. Based on these experimental findings, it is concluded that DA improves reproductive performance of aged roosters.