Investigation of nitrogen loss during laboratory scale fixed-bed drying of digestate.

In addition to the drying kinetics of digestate, a comprehensive knowledge is necessary on nitrogen loss and ammonia emissions in order to appropriately develop and design a dryer. Otherwise, these phenomena would result in environmental concerns and reduce the nutrient value of digestate. Expectedly, drying parameters considerably affect drying time. In this study, drying experiments were conducted at a laboratory scale fixed-bed dryer. The drying experiments were performed on dewatered digestate that mainly consisted of grass and maize silage (initial moisture content 76.4% w.b., 0.026 kg N kg-1 DM). The drying air temperatures were adjusted to 60, 70 and 80 °C, the airflow rate varied between 150 and 250 m3 h-1, respectively. As the results have shown, the total nitrogen was reduced by 29 to 42% during drying. Almost all ammonium nitrogen was lost (92%). No relationship between nitrogen loss and drying air temperature or airflow rate could be proved. These results will be used as a basis for further investigation of continuous digestate drying at a semi-technical scale.

Methane prediction based on individual or groups of milk fatty acids for dairy cows fed rations with or without linseed.

Milk fatty acids (MFA) are a proxy for the prediction of CH4 emission from cows, and prediction differs with diet. Our objectives were (1) to compare the effect of diets on the relation between MFA profile and measured CH4 production, (2) to predict CH4 production based on 6 data sets differing in the number and type of MFA, and (3) to test whether additional inclusion of energy-corrected milk (ECM) yield or dry matter intake (DMI) as explanatory variables improves predictions. Twenty dairy cows were used. Four diets were used based on corn silage (CS) or grass silage (GS) without (L0) or with linseed (LS) supplementation. Ten cows were fed CS-L0 and CS-LS and the other 10 cows were fed GS-L0 and GS-LS in random order. In feeding wk 5 of each diet, CH4 production (L/d) was measured in respiration chambers for 48 h and milk was analyzed for MFA concentrations by gas chromatography. Specific CH4 prediction equations were obtained for L0-, LS-, GS-, and CS-based diets and for all 4 diets collectively and validated by an internal cross-validation. Models were developed containing either 43 identified MFA or a reduced set of 7 groups of biochemically related MFA plus C16:0 and C18:0. The CS and LS diets reduced CH4 production compared with GS and L0 diets, respectively. Methane yield (L/kg of DMI) reduction by LS was higher with CS than GS diets. The concentrations of C18:1 trans and n-3 MFA differed among GS and CS diets. The LS diets resulted in a higher proportion of unsaturated MFA at the expense of saturated MFA. When using the data set of 43 individual MFA to predict CH4 production (L/d), the cross-validation coefficient of determination (R2CV) ranged from 0.47 to 0.92. When using groups of MFA variables, the R2CV ranged from 0.31 to 0.84. The fit parameters of the latter models were improved by inclusion of ECM or DMI, but not when added to the data set of 43 MFA for all diets pooled. Models based on GS diets always had a lower prediction potential (R2CV = 0.31 to 0.71) compared with data from CS diets (R2CV = 0.56 to 0.92). Models based on LS diets produced lower prediction with data sets with reduced MFA variables (R2CV = 0.62 to 0.68) compared with L0 diets (R2CV = 0.67 to 0.80). The MFA C18:1 cis-9 and C24:0 and the monounsaturated FA occurred most often in models. In conclusion, models with a reduced number of MFA variables and ECM or DMI are suitable for CH4 prediction, and CH4 prediction equations based on diets containing linseed resulted in lower prediction accuracy.

Abdome agudo: diagnóstico sindrômico / Acute abdome: syndromic diagnosis

O objetivo do capítulo é facilitar o diagnostico etiológico do quadro de abdome agudo na sala de emergência. Será apresentado um algoritmo para melhor sistematização do diagnóstico, uma vez que abdome agudo é um dos motivos mais comuns que levam um paciente ao atendimento de emergência.

The purpose of this work is to facilitate etiologic diagnosis of acute abdomen in the emergency room. Showing an algorithm to better systematize of the diagnosis, since acute abdomen is one of the most common reasons that lead a patient to emergency care.

Fraturas expostas: classificação e manejo / Exposed fractures: classification and management

Apesar de bastante comuns no dia-a-dia de profissionais que lidam com o atendimento de trauma, as fraturas expostas despertam muitas dúvidas, principalmente aos olhos de profissionais não especialistas que se deparam diariamente com estes casos nas emergências. Este artigo traz uma revisão e um guia para o diagnóstico e o manejo dessas lesões.

Although very common between professionals that deal with trauma care on a daily basis, open fractures bring up several questions, mainly when seen through the eyes of non-specialists who face these cases on emergencies every day. This paper brings a review and a guide for diagnosis and management of these injuries.

Monitoring the body temperature of cows and calves using video recordings from an infrared thermography camera.

The aim of this study was to assess the variability of temperatures measured by a video-based infrared camera (IRC) in comparison to rectal and vaginal temperatures. The body surface temperatures of cows and calves were measured contactless at different body regions using videos from the IRC. Altogether, 22 cows and 9 calves were examined. The differences of the measured IRC temperatures among the body regions, i.e. eye (mean: 37.0 °C), back of the ear (35.6 °C), shoulder (34.9 °C) and vulva (37.2 °C), were significant (P < 0.01), except between eye and vulva (P = 0.99). The quartile ranges of the measured IRC temperatures at the 4 above mentioned regions were between 1.2 and 1.8 K. Of the investigated body regions the eye and the back of the ear proved to be suitable as practical regions for temperature monitoring. The temperatures of these 2 regions could be gained by the use of the maximum temperatures of the head and body area. Therefore, only the maximum temperatures of both areas were used for further analysis. The data analysis showed an increase for the maximum temperature measured by IRC at head and body area with an increase of rectal temperature in cows and calves. The use of infrared thermography videos has the advantage to analyze more than 1 picture per animal in a short period of time, and shows potential as a monitoring system for body temperatures in cattle.

Ammonia emissions from a broiler farm: spatial variability of airborne concentrations in the vicinity and impact on adjacent woodland.

Agricultural NH(3) emissions affect air quality and influence the nitrogen cycle. In the subject study, NH(3) emissions from a broiler farm and the resulting atmospheric concentrations in the immediate vicinity during three growing cycles have been quantified. Additionally, vegetation along a transect in an adjacent woodland was analysed. The emissions were as high as 10 kg NH(3) h(-1) and the atmospheric concentrations ranged between 33 and 124 µg NH(3) m(-3) per week in the immediate vicinity. Measurements of the atmospheric concentrations over 7 weeks showed a substantial decline of mean concentrations (based on a 3-week average) from ∼13 to <3 µg NH(3) m(-3), at 45- and 415-m distance from the farm. Vegetation surveys showed that nitrophilous species flourished when they grew closest to the farm (their occurrence sank proportionately with distance). A clearly visible damage of pine trees was observed within 200 m of the farm; this illustrated the significant impact of NH(3) emissions from agricultural sources on the sensitive ecosystem.

Antigenic properties of the human immunodeficiency virus transmembrane glycoprotein during cell-cell fusion.

Human immunodeficiency virus (HIV) entry is triggered by interactions between a pair of heptad repeats in the gp41 ectodomain, which convert a prehairpin gp41 trimer into a fusogenic three-hairpin bundle. Here we examined the disposition and antigenic nature of these structures during the HIV-mediated fusion of HeLa cells expressing either HIV(HXB2) envelope (Env cells) or CXCR4 and CD4 (target cells). Cell-cell fusion, indicated by cytoplasmic dye transfer, was allowed to progress for various lengths of time and then arrested. Fusion intermediates were then examined for reactivity with various monoclonal antibodies (MAbs) against immunogenic cluster I and cluster II epitopes in the gp41 ectodomain. All of these MAbs produced similar staining patterns indicative of reactivity with prehairpin gp41 intermediates or related structures. MAb staining was seen on Env cells only upon exposure to soluble CD4, CD4-positive, coreceptor-negative cells, or stromal cell-derived factor-treated target cells. In the fusion system, the MAbs reacted with the interfaces of attached Env and target cells within 10 min of coculture. MAb reactivity colocalized with the formation of gp120-CD4-coreceptor tricomplexes after longer periods of coculture, although reactivity was absent on cells exhibiting cytoplasmic dye transfer. Notably, the MAbs were unable to inhibit fusion even when allowed to react with soluble-CD4-triggered or temperature-arrested antigens prior to initiation of the fusion process. In comparison, a broadly neutralizing antibody, 2F5, which recognizes gp41 antigens in the HIV envelope spike, was immunoreactive with free Env cells and Env-target cell clusters but not with fused cells. Notably, exposure of the 2F5 epitope required temperature-dependent elements of the HIV envelope structure, as MAb binding occurred only above 19 degrees C. Overall, these results demonstrate that immunogenic epitopes, both neutralizing and nonneutralizing, are accessible on gp41 antigens prior to membrane fusion. The 2F5 epitope appears to depend on temperature-dependent elements on prefusion antigens, whereas cluster I and cluster II epitopes are displayed by transient gp41 structures. Such findings have important implications for HIV vaccine approaches based on gp41 intermediates.