Subject(s)
Anti-Bacterial Agents/pharmacology , Pneumococcal Infections/microbiology , Streptococcus pneumoniae/drug effects , Drug Resistance, Microbial , Drug Resistance, Multiple , Humans , Microbial Sensitivity Tests/methods , Pneumococcal Infections/epidemiology , Streptococcus pneumoniae/isolation & purification , Turkey/epidemiologyABSTRACT
The persistent presence of bacteria in the root canal system often leads to the failure of treatment. The purpose of this study was to determine in vitro penetration of Streptococcus sanguis and Prevotella intermedia into dentinal tubules. Samples obtained from human teeth were inoculated with a strain of S. sanguis (NCTC 7853) and P. intermedia (NCTC 93336) for 20 days. Bacterial penetration into tubules was investigated at scanning electron microscopy and light microscopic level. The results showed that S. sanguis could penetrate into dentinal tubules 382.3 microns, whereas P. intermedia could penetrate 25.9 microns. It was observed that P. intermedia had not penetrated into all dentinal tubules. If penetration occurred the depth was quite limited.
Subject(s)
Dentin/microbiology , Prevotella intermedia/physiology , Streptococcus sanguis/physiology , Colony Count, Microbial , Culture Media , Culture Techniques , Dental Cementum/microbiology , Dental Pulp Cavity/microbiology , Dentin/ultrastructure , Humans , Microscopy, Electron, Scanning , Smear LayerABSTRACT
The antibacterial effects of the Nd:YAG laser on contaminated root canals and dentinal tubules were observed as the aim of this study. The samples were inoculated with Streptococcus sanguis (NCTC 7853) and Prevotella intermedia (NCTC 93336), and the effects of Nd:YAG laser were tested on these teeth. The specimens were lased with 1.8 W and 2.4 W Nd:YAG laser for 30 s, and the presence of bacteria in tubules was observed under light microscopy. The 1.8 W laser sterilized the tubules in 86.3% of sections inoculated with S. sanguis, whereas 2.4 W laser sterilized in 98.5% of the sections. Both laser powers sterilized all samples inoculated with P. intermedia. The scanning electron microscopic observations supported the light microscopic findings.
