ABSTRACT
We present temperature-dependent single-crystal diffraction results on seven antifluorite-typeA2MeX6compounds withMe= Os or Ir: K2OsCl6,A2OsBr6withA= K, Rb, Cs and NH4, and K2IrX6withX= Cl and Br. The structural transitions in this family arise fromMeX6octahedron rotations that generate a rich variety of symmetries depending on the rotation axis and stacking schemes. In order to search for local distortions in the high-symmetry phase we perform refinements of anharmonic atomic displacement parameters with comprehensive data sets. Even at temperatures close to the onset of structural distortions, these refinements only yield a small improvement indicating only small anharmonic effects. The phase transitions in these antifluorites are essentially of displacive character. However, some harmonic displacement parameters are very large reflecting soft phonon modes with the softening covering large parts of the Brillouin zone. The occurrence of the rotational transitions in the antifluorite-type family can be remarkably well analyzed in terms of a tolerance factor of ionic radii.
ABSTRACT
The ease of prescribing radiological examinations has prompted an expansion in radiological procedures and, consequently, an increase of occupational dose to medical imaging workers. However, little is known about radiation exposure in the workplace of medical radiology professionals in many countries, and in Benin particularly. The purpose of this study was to assess ambient radiation doses in diagnostic X-ray medical facilities in Benin and to observe whether exposure levels are below reference levels. A total of 72 public and private medical imaging centres participated in a cross-sectional study carried out from June 2019 to February 2020 in Benin. These centres had 59 X-ray, four chest and six computed tomography (CT) scan rooms. A calibrated radiameter able to measure short, pulsed or continuous X fields and gamma/beta (50 nSv to 10 Sv) was used to measure exposure levels in these functional rooms. Scattered X-ray doses and exposure time from radiological examinations both behind the lead glass of the control area to assess the levels of exposure of professionals and outside of the examination room to evaluate the level of exposure of the public (including non-exposed workers) have been provided. Equivalent doses estimated per hour were compared with the reference levels of 7.50 and 0.05 µSv per hour for workers and the public, respectively. At the control area, the mean/median (min-max) equivalent doses were 0.09/0.07 (0.00-0.21), 2.39/0.13 (0.00-75.67), and 228.39/28.65 (0.39-869.75) µSv per hour for the chest, X-ray, and CT-scan rooms, respectively. Among 69 examination rooms, 13.04% of the equivalent dose estimated in the workplace behind the lead glass was greater than 7.50 µSv per hour; 65 out of 69 examination rooms showed that 40.00% of the equivalent dose estimated behind the doors was greater than 0.05 µSv per hour. These results demonstrated that current controls, including leaded glass separating the control panel and leaded doors between the examination room and the corridor, are inadequate to limit radiation exposures. The controls must be upgraded and a dosimetry program should be implemented to monitor exposures of employees, patients, and visitors.
Subject(s)
Occupational Exposure , Radiation Exposure , Benin , Cross-Sectional Studies , Humans , Occupational Exposure/analysis , Radiation Dosage , Radiography , WorkplaceABSTRACT
The present study examined in lambs whether exposure to flavors derived from pregnant mother's diet and transferred to amniotic fluid (AF) could induce a preference for artificial milk containing one of these flavors. To test this hypothesis, cumin was added to the maternal diet in the last month of gestation. Preference for artificial milk containing p-cymene, one of the chemosensory compounds of cumin, was tested within the first two days after birth in maternally deprived lambs born from mothers fed a cumin-flavored diet (Cumin group), or an unflavored diet (Control group). Aromatic profile of AF from cumin-fed mothers was analyzed by GC-MS/MS to determine whether p-cymene could be detected. While the control group avoided the flavored artificial milk on day 1, the Cumin group did not and showed a preference for the cumin-scented formula on day 2. GC-MS/MS profile of AF revealed that four of the main volatile cumin compounds, p-cymene, p-cymenene, ß-pinene and γ-terpinene were present in variable amounts in all samples, p-cymene being the most frequently detected. These findings indicate that newborn lambs can memorize flavors from the mother's diet present in AF and that prenatal experience influences their preference for an artificial milk containing one specific flavor.
Subject(s)
Food Preferences , Milk , Amniotic Fluid , Animals , Animals, Newborn , Diet , Female , Pregnancy , Sheep , Tandem Mass SpectrometryABSTRACT
PURPOSE OF THE REVIEW: The aim of this review is to discuss recent data pointing at an involvement of human endogenous retroviruses (HERVs) in type 1 diabetes (T1D) onset and progression. RECENT FINDINGS: The envelope protein of HERV-W family, named HERV-W-Env, was detected in pancreata from T1D patients and was shown to display pro-inflammatory properties and direct toxicity toward pancreatic beta cells. The etiopathogenesis of T1D remains elusive, even if conventional environmental viral infections have been recurrently involved. Nonetheless, a new category of pathogens may provide the missing link between genetic susceptibility and environmental factors long thought to contribute to T1D onset. A number of studies have now shown that HERV sequences, which are normally inactivated or repressed in the human genome, could be activated by environmental viruses. Thus, if similarly activated by viruses associated with T1D, disregarded HERV genes may underlie T1D genetic susceptibility. Moreover, once expressed, HERV elements may display broad pathogenic properties, which identify them as potential new therapeutic targets.
Subject(s)
Diabetes Mellitus, Type 1/etiology , Endogenous Retroviruses/physiology , Gene Products, env/isolation & purification , Insulin-Secreting Cells/virology , Virus Activation/physiology , Animals , Autoimmune Diseases/etiology , Autoimmune Diseases/physiopathology , Diabetes Mellitus, Type 1/genetics , Diabetes Mellitus, Type 1/virology , Disease Models, Animal , Disease Progression , Endogenous Retroviruses/isolation & purification , Endogenous Retroviruses/pathogenicity , Epigenesis, Genetic , Gene-Environment Interaction , Humans , MiceABSTRACT
Temperature is an important climate factor that has a direct influence on insect biology and consequently a crucial role in forecasting and integrated pest management. The mealybug Dysmicoccus brevipes (Cockerell) (Hemiptera: Pseudococcidae) is one of the most common species in Brazilian vineyards. Here, development and survival of D. brevipes on leaves of table grapes (Vitis vinifera cv. Itália) were studied at five constant temperatures (15, 20, 25, 28, and 30 ± 1°C) under laboratory conditions. We investigated the developmental time and nymphal survival, temperature thresholds, and the degree-day requirements for each life stage of D. brevipes. The developmental time for the nymphal stage ranged from 32 to 130 days with decreasing the temperature, with the temperatures of 28 and 30°C providing the shorter developmental time. Survival of the entire nymphal stage was affected by the temperature, ranging from 81% at 20°C to 1% at 15°C. The predicted upper lethal temperature was 34.5°C, while the optimal temperature was 28.6°C. The minimum threshold for total development of D. brevipes occurred at 8.2°C. Dysmicoccus brevipes required 678.4 degree-days to complete development from first-instar nymph to adult. The temperature of 30°C was the most suitable for the development of D. brevipes. The thermal thresholds estimated for D. brevipes life cycle suggest that this species can develop in different table grape-producing regions of Brazil, in a temperature range between 8 and 35°C.
Subject(s)
Hemiptera/growth & development , Life Cycle Stages , Temperature , Vitis , Animals , Brazil , Female , Fruit , Pest ControlABSTRACT
INTRODUCTION: Myeloid sarcomas are uncommon proliferations of immature myeloid cells occurring in any extramedullary organ. We report here two cases of myeloid sarcomas in patients with, respectively, a polycythemia vera and a myelodysplastic syndrome. CASE REPORTS: The first is an 81-year-old woman who presented with osteolytic lesions. Diagnosis has been highlighted using anatomopathological study after bone marrow biopsy, but it was delayed because of a very localized basin lesion and few positive myeloid markers. The second patient is an 86-year-old man who presented with pancytopenia and several lymph nodes. Lymph node cytology failed because of the rarity of blast cells. Diagnosis was done after anatomopathological study on lymph node biopsy which revealed a localized form of myeloid sarcoma. CONCLUSION: The diagnosis of myeloid sarcoma must be considered when unusual tumors occur in patients with a chronic myeloid disease. In that case, therapeutic options are those of an acute myeloid leukemia.
Subject(s)
Sarcoma, Myeloid/diagnosis , Aged, 80 and over , Biopsy , Bone Marrow/pathology , Diagnosis, Differential , Female , Humans , Leukemia, Myeloid, Acute/diagnosis , Male , Myelodysplastic Syndromes/complications , Myelodysplastic Syndromes/diagnosis , Sarcoma, Myeloid/etiologyABSTRACT
Septins are highly conserved and essential eukaryotic cytoskeletal proteins that interact with the inner plasma membrane. They are involved in essential functions requiring cell membrane remodeling and compartmentalization, such as cell division and dendrite morphogenesis, and have been implicated in numerous diseases. Depending on the organisms and on the type of tissue, a specific set of septins genes are expressed, ranging from 2 to 13. Septins self-assemble into linear, symmetric rods that can further organize into linear filaments several microns in length. Only a subset of human septins has been described at high resolution by X-ray crystallography (Sirajuddin et al., 2007). Electron microscopy (EM) has proven to be a method of choice for analyzing the molecular organization of septins. It is possible to localize each septin subunit within the rod complex using genetic tags, such as maltose-binding protein or green fluorescent protein, to generate a visible label of a specific septin subunit in EM images that are processed using single-particle EM methodology. In this chapter we present, in detail, the methods that we have used to analyze the molecular organization of budding yeast septins (Bertin et al., 2008). These methods include purification of septin complexes, sample preparation for EM, and image processing procedures. Such methods can be generalized to analyze the organization of septins from any organism.
Subject(s)
Cytoskeleton/ultrastructure , Microscopy, Electron/methods , Multiprotein Complexes/isolation & purification , Recombinant Proteins/isolation & purification , Septins/isolation & purification , Crystallography, X-Ray , Cytoskeleton/chemistry , Green Fluorescent Proteins , Multiprotein Complexes/chemistry , Multiprotein Complexes/genetics , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Saccharomyces cerevisiae/chemistry , Saccharomyces cerevisiae/ultrastructure , Septins/chemistry , Septins/geneticsABSTRACT
Feces-based population genetic studies have become increasingly popular. However, polymerase chain reaction (PCR) amplification rates from fecal material vary depending on the species, populations, loci, and extraction protocols. Here, we assessed the PCR amplification success of three microsatellite markers and a segment of the mitochondrial control region of DNA extracted from field-collected feces of guanaco (Lama guanicoe) using two protocols - Qiagen DNA Stool Kit and 2 cetyltrimethylammonium bromide/phenol:chloroform:isoamyl alcohol (2CTAB/PCI) method. Chelex resin treatment to remove inhibitors was also tested. Our results show that the mitochondrial locus was the most difficult to amplify. PCR success rates improved for all markers after Chelex treatment of extracted DNA, and 2CTAB/PCI method (95.83%) appeared to perform slightly better than stool kit (91.67%) for the nuclear markers. Amplification success was significantly influenced by the extraction method, Chelex treatment, and locus (P < 0.001) but not by the freshness of the feces (fresh vs old, P = 0.17). The repeatability levels were high without Chelex treatment (> 0.89), but they decreased slightly after treatment for amplification of nuclear markers and markedly after treatment for amplification of the mitochondrial control region. Thus, we showed that Chelex treatment gives high PCR success, especially for nuclear markers, and adequate DNA extraction rates can be achieved from L. guanicoe feces even from non-fresh fecal material. Although not significant, 2CTAB/PCI method tended to provide higher successful amplification rates on a whole set of samples, suggesting that the method could be particularly useful when using small sample sizes.
Subject(s)
Camelids, New World/genetics , DNA/isolation & purification , Feces/chemistry , Animals , DNA/genetics , Genetic Loci , Polymerase Chain Reaction/methods , Reproducibility of ResultsABSTRACT
Compared to rodents, the relationship between anxiety and cognitive performances has been less studied in birds. Yet, birds are frequently exposed to stimulations that constitute a potential source of anxiety and can affect their adaptation to their living conditions. The present study was aimed at evaluating, in birds, the relationship between levels of anxiety and object habituation and discrimination with the use of Japanese quail lines divergently selected for a fear response, tonic immobility. Previous studies demonstrated that the selection programme has modified the general anxiety trait of the birds. The task consisted in 4 daily sessions of 8 successive presentations of the same object in the home cage of the quail in order to habituate each bird to the object. The observation that both quail with a high and a low anxiety trait progressively spent more time close to the object indicated that habituation occurred. Dishabituation was assessed during a single session of 8 presentations of a novel object. Only quail with a high anxiety trait exhibited significant discrimination. They spent significantly less time close to the novel object than to the habituated object. It is hypothesised that a high anxiety trait is associated with a more accurate processing of environmental cues or events resulting in better discriminative performances.
Subject(s)
Anxiety/genetics , Anxiety/physiopathology , Discrimination, Psychological/physiology , Habituation, Psychophysiologic/physiology , Analysis of Variance , Animals , Anxiety/psychology , Coturnix , Habituation, Psychophysiologic/genetics , MaleABSTRACT
This study tested whether lines of Japanese quails divergently selected for a fear response, the tonic immobility, might constitute a reliable bird model for studying anxiety. Previous studies demonstrated that the selection modifies the general underlying emotionality of the birds rather than exerting its effect only on tonic immobility. The behavioural effects of intraperitoneal injections of diazepam, an anxiolytic drug, were assessed in two lines of quail selected either for their short (STI) or long (LTI) duration of tonic immobility. Effects of diazepam were examined in two tests used for measuring emotionality in birds, the open field and the tonic immobility tests. After being placed in the centre of the open field, birds with a high emotionality (LTI quails) stayed longer in the centre of the apparatus than STI quail. Diazepam had anxiolytic effect in LTI birds as it increased the time spent in the outer area. This effect of diazepam appears to be selective because the drug has no effect on other behaviours such as distress calls or escape attempts. The drug has also no effect on the tonic immobility response in any of the two lines. These findings reveal an "anxiogenic" trait of LTI birds in the open field test that can be modulated by the administration of an anxiolytic drug. Therefore quails selected for LTI and STI represent a valuable model to study the mechanisms underlying anxiety in birds.
Subject(s)
Anti-Anxiety Agents/therapeutic use , Anxiety/drug therapy , Diazepam/therapeutic use , Fear/drug effects , Analysis of Variance , Animals , Anti-Anxiety Agents/pharmacology , Anxiety/genetics , Behavior, Animal/drug effects , Coturnix , Diazepam/pharmacology , Disease Models, Animal , Dose-Response Relationship, Drug , Exploratory Behavior/drug effects , Female , Statistics, Nonparametric , Time FactorsABSTRACT
Using a simple scaling law, a unique set of crystal-field parameters for the rare-earth ions R in the R(2)Ti(2)O(7) pyrochlore series is shown to provide a proper description of the crystal-field excitations previously observed by neutron scattering. The two spectroscopic g factors are given for the compounds with doublet ground states, as well as the ground-state wavefunctions for all the compounds.
ABSTRACT
There is considerable variability in the susceptibility of individuals to the adverse effects of chronic stress. In humans and other mammals, individual traits such as high anxiety are proposed as a vulnerability factor for the development of stress-related disorders. In the present study, we tested whether a similar behavioural trait in birds, higher emotional reactivity, also favours the occurrence of chronic stress-related behavioural and physiological dysfunction. For this, lines of Japanese quail divergently selected for a typical fear response in birds, the duration of tonic immobility, were subjected to unpredictable aversive stimulation over 2 weeks. Previous studies demonstrate that the selection program modifies the general underlying emotionality of the birds rather than exerting its effect only on tonic immobility. Interestingly, only birds selected for their higher emotionality exhibited significantly enhanced latency to first step and decreased locomotor activity in the open-field test after exposure to chronic stress compared to non-stressed control birds. This effect of chronic stress was selective for the tested dimension of bird emotional reactivity because there was no observed effect on the tonic immobility response. Moreover, chronically stressed birds selected for their higher emotionality exhibited significantly decreased basal corticosterone levels, a physiological marker of stress. These findings show that chronic stress is associated with changes in emotional reactivity and related physiological markers in birds. They also highlight emotional reactivity as an important predisposing factor for the occurrence of the adverse effects of chronic stress in birds.
Subject(s)
Coturnix/genetics , Fear/psychology , Selection, Genetic/physiology , Stress, Psychological/psychology , Animals , Body Weight/physiology , Corticosterone/blood , Coturnix/blood , Coturnix/physiology , Fear/physiology , Female , Immobility Response, Tonic/physiology , Motor Activity , Stress, Psychological/blood , Stress, Psychological/physiopathologyABSTRACT
The grafting of pegylated dendrons on 9(2) nm and 39(5) nm iron oxide nanoparticles in water, through a phosphonate group as coupling agent has been successfully achieved and its mechanism investigated, with a view to produce biocompatible magnetic nano-objects for biomedical applications. Grafting has been demonstrated to occur by interaction of negatively charged phosphonate groups with positively charged groups and hydroxyl at the iron oxide surface. The isoelectric point of the suspension of dendronized iron oxide nanoparticles is shifted towards lower pH as the amount of dendron increases. It reaches 4.7 for the higher grafting rate and for both particle size. Thus, the grafting of molecules using a phosphonate group allows stabilizing electrostatically the suspensions at physiological pH, a prerequisite for biomedical applications. Moreover the grafting step has been shown to preserve the magnetic properties of iron oxide nanoparticles due to super-super exchange interactions through the phosphonate group.
ABSTRACT
OBJECTIVE: To assess the awareness and attitude towards AIDS and PLHA in slum dwellers of Chennai, an Indian metropolis by KAP (Knowledge, Attitude, Practice) study. METHODS: A cross sectional study was conducted in a representative sample of 650 subjects (400 females and 250 females), aged 15-45 years, by means of a questionnaire in the local dialect Tamil. RESULTS: The overall literacy rate was 64%, with males being 70% and females being 60% literate. 20% of males and 11% of females do not know about a disease called AIDS. Only 67% of males and 55% of females are aware of the sexual mode of transmission. 34% of males and 50% females opine that AIDS is also a hereditary disease. Also 45% of males and 62% of females feel that AIDS also spreads by air, fomites, or mosquito-bite. Only 30% of males and 22% females know about the possible symptoms of AIDS. 30% of males and 45% of females never ask for a new syringe if not provided, as they are totally unaware of its significance. 43% of males and 78% of females do not know about the risk of a barber's blade. 56% of males and 71% of females feel that AIDS can be treated at least by a traditional medicine. Lastly, 48% of males and 60% females prefer outcasting an AIDS patient from the slum. CONCLUSIONS: AIDS awareness in the slum dwellers of Chennai is very poor. Corresponding awareness in suburbs and rural areas will be much worse. Conventional IEC methods targeting general population via mass media are not reaching the slum dwellers, even in a metropolitan city. A specially designed targeted intervention is needed.
Subject(s)
Acquired Immunodeficiency Syndrome/psychology , Attitude to Health , Health Knowledge, Attitudes, Practice , Poverty Areas , Urban Population , Acquired Immunodeficiency Syndrome/transmission , Adolescent , Adult , Barbering , Cross-Sectional Studies , Educational Status , Female , Health Education , Herbal Medicine , Humans , India , Male , Medicine, Traditional , Middle Aged , Risk Factors , SyringesABSTRACT
In beet distilleries, condensates arising from stillage concentration could be recycled as dilution water for the fermentation step, thus preserving groundwater resources and ensuring a quality-controlled water supply. However, the recycling of condensates has been found to cause a significant reduction in fermentation activity. This study aimed to verify that condensates are toxic to alcoholic fermentation. Ten compounds found in condensates (formic, acetic, propionic, butyric, valeric, and hexanoic acids; 2,3-butanediol, furfuryl alcohol, furfural, and 2-phenyl-ethyl-alcohol) were tested. With the exception of 2,3-butanediol, they all proved to be inhibitors. At the same molar concentration, the longer the carbonaceous chain, the stronger the inhibition by fatty acids. An experimental design was used to study the inhibitory characteristics of the 10 compounds at the concentrations found in condensates. Synergistic effects were also confirmed. In real effluents, acetic acid was so highly concentrated that it became the strongest inhibitor. It is therefore necessary to eliminate it before recycling, as well as less concentrated compounds that may accumulate, as illustrated by the simulation.
Subject(s)
Alcohols/metabolism , Conservation of Natural Resources , Fermentation , Food Microbiology , Acids, Acyclic/pharmacology , Butylene Glycols/pharmacology , Fatty Acids/pharmacology , Furaldehyde/pharmacology , Furans/pharmacology , Phenylethyl Alcohol/pharmacology , Time FactorsABSTRACT
OBJECTIVE: This study has been carried out to assess the effect of tobacco smoking and of betel quid chewing with tobacco on buccal mucosa by cytomorphometry, in a south Indian population. STUDY DESIGN: Cellular diameter (CD) and nuclear diameter (ND) of exfoliated buccal squames obtained from clinically normal appearing buccal mucosa of tobacco smokers, betel quid with tobacco chewers, and those with a combined habit, stained by the Papanicolaou method, were measured. Non-users served as negative controls and oral squamous cell carcinomas in tobacco users served as positive controls. One way ANOVA test of the values obtained followed by multiple range comparison with Tukey-HSD procedure (at p=0.05) was carried out. RESULTS: A statistically significant reduction in CD and increase in ND in smokers and those with a combined habit were observed. CONCLUSION: The use of tobacco in the form of smoking influences the cytomorphology of buccal mucosa.
Subject(s)
Mouth Mucosa/pathology , Smoking/pathology , Areca/adverse effects , Carcinoma, Squamous Cell/pathology , Cell Nucleus/ultrastructure , Cell Size , Coloring Agents , Cytological Techniques , Epithelial Cells/pathology , Female , Humans , Male , Mouth Neoplasms/pathology , Smoking/adverse effects , Tobacco, Smokeless/adverse effectsABSTRACT
While congruent evidence indicates that sexual selection is the most likely selective force explaining the rapid divergence of male genital morphology in insects, the mechanisms involved in this process remain unclear. In particular, little attention has been paid to precopulatory sexual selection. We examine sexual selection for mating success on male genital components in six populations of Aquarius remigis, a water strider characterized by unique genital morphology. Multivariate selection analysis confirms previous findings that precopulatory sexual selection favours longer external genitalia, and provides new evidence that this selection acts independently on external genital components. In contrast, the size of the major internal genital sclerite is not correlated with mating success. Thus, precopulatory sexual selection acts strongly on the size of the external genitalia, but not on the intromittent organ itself. These results highlight the multiple functions of genital organs and the importance of both precopulatory and post-copulatory sexual selection in shaping the remarkable diversity of male genitalia in insects.
Subject(s)
Copulation/physiology , Genitalia, Male/anatomy & histology , Heteroptera/anatomy & histology , Selection, Genetic , Animals , Body Weights and Measures , California , Heteroptera/physiology , Male , Multivariate AnalysisSubject(s)
Antibodies, Monoclonal/adverse effects , Antibodies, Monoclonal/therapeutic use , Antineoplastic Agents/adverse effects , Antineoplastic Agents/therapeutic use , Hair Diseases/chemically induced , Aged , Antibodies, Monoclonal, Humanized , Cetuximab , Eyelashes/drug effects , Eyelashes/growth & development , Eyelashes/pathology , Female , Humans , Rectal Neoplasms/drug therapyABSTRACT
We have searched for Theta+(1540) and Xi(--)(1862) pentaquark candidates in proton-induced reactions on C, Ti, and W targets at midrapidity and square root of s = 41.6 GeV. In 2 x 10(8) inelastic events we find no evidence for narrow (sigma approximately 5 MeV) signals in the Theta+ --> pK0(S) and Xi(--) --> Xi- pi- channels; our 95% C.L. upper limits (UL) for the inclusive production cross section times branching fraction B dsigma/dy/(y approximately 0) are (4-16) mub/N for a Theta+ mass between 1521 and 1555 MeV, and 2.5 mub/N for the Xi(--). The UL of the yield ratio of Theta+/Lambda(1520) < (3-12)% is significantly lower than model predictions. Our UL of B Xi(--)/Xi(1530)0 < 4% is at variance with the results that have provided the first evidence for the Xi(--).
ABSTRACT
In crustacean species with precopulatory mate-guarding, sexual size dimorphism has most often been regarded as the consequence of a large male advantage in contest competition for access to females. However, large body size in males may also be favoured indirectly through scramble competition. This might partly be the case if the actual target of selection is a morphological character, closely correlated with body size, involved in the detection of receptive females. We studied sexual selection on body size and antennae length in natural populations of Asellus aquaticus, an isopod species with precopulatory mate guarding. In this species, males are larger than females and male pairing success is positively related to body size. However, males also have longer antennae, relative to body size, than females, suggesting that this character may also be favoured by sexual selection. We used multivariate analysis of selection to assess the relative influences of body size and antennae length in five different populations in the field. Selection gradients indicated that overall body size was a better predictor of male pairing success than antennae length, although some variation was observed between sites. We then manipulated male antennae length in a series of experiments conducted in the laboratory, and compared the pairing ability of males with short or long antennae. Males with short antennae were less likely to detect, orient to and to pair with a receptive female compared to males with long antennae. We discuss the implications of our results for studies of male body size and sexual dimorphism in relation to sexual selection in crustaceans.