Lethal and sublethal effects of chlorantraniliprole on Spodoptera cosmioides (Lepidoptera: Noctuidae).

BACKGROUND: The Spodoptera cosmioides (Walker, 1858) population has increased in Bacillus thuringiensis Berliner (Bt) soybean crops in Argentina. As there are no registered products for its control, the recommended insecticides for S. frugiperda are used. The aim of this study was therefore to determine the lethal concentration (LC) of chlorantraniliprole and its sublethal effects on the biological and reproductive functions of S. cosmioides, an emerging soybean pest in Argentina. RESULTS: An ingestion toxicity bioassay showed that chlorantraniliprole was active against larvae of the second instar, and after 48 h of exposure LC50 was 0.054 µg mL-1 H2 O. In the study of sublethal effect, chlorantraniliprole induced changes in the life cycle of exposed S. cosmioides, which required more time to complete all stages of development (larval, pupal and adult stages). Pupal weight was also higher in larvae exposed to sublethal concentrations of chlorantraniliprole. Adult fecundity was decreased: the number of eggs laid by each adult female moth, as compared with control females, was two (LC15 ) and eight (LC30 ) times lower. CONCLUSION: These results indicate that chlorantraniliprole has toxicity against S. cosmioides larvae. Sublethal effects on the biological and reproductive performance of this species can help optimize integrated pest management programs. © 2018 Society of Chemical Industry.

Mortality of Plutella xylostella (Lepidoptera, Plutellidae) by parasitoids in the Province of Santa Fe, Argentina

Mortality of Plutella xylostella (Lepidoptera, Plutellidae) by parasitoids in the Province of Santa Fe, Argentina. Plutella xylostella (Linnaeus, 1758) (Lepidoptera, Plutellidae) larvae cause severe economic damage on cabbage, Brassica oleracea L. variety capitata (Brassicaceae), in the horticultural fields in the Province of Santa Fe, Argentina. Overuse of broad spectrum insecticides affects the action of natural enemies of this insect on cabbage. The objectives of this work were to identify the parasitoids of P. xylostella and to determine their influence on larva and pupa mortality. Weekly collections of larvae and pupae were randomly conducted in cabbage crops during spring 2006 and 2007. The immature forms collected were classified according to their developmental stage: L1 and L2 (Ls = small larvae), L3 (Lm = medium larvae), L4 (Ll = large larvae), pre-pupae and pupae (P). Each individual was observed daily in the laboratory until the adult pest or parasitoid emergence. We identified parasitoids, the number of instar and the percentage of mortality of P. xylostella for each species of parasitoid. Parasitoids recorded were: Diadegma insulare (Cresson, 1875) (Hymenoptera, Ichneumonidae), Oomyzus sokolowskii (Kurdjumov, 1912) (Hymenoptera, Eulophidae), Cotesia plutellae (Kurdjumov, 1912) (Hymenoptera, Braconidae) and an unidentified species of Chalcididae (Hymenoptera). Besides parasitoids, an unidentified entomopathogenic fungus was also recorded in 2006 and 2007. In 2006, the most successful parasitoids were D. insulare and O. sokolowskii, while in 2007 only D. insulare exerted a satisfactory control and it attacked the early instars of the pest.

Mortalidade de Plutella xylostella (Lepidoptera, Plutellidae) por parasitóides na Província de Santa Fé, Argentina. Plutella xylostella (Linnaeus, 1758) (Lepidoptera, Plutellidae) causa danos econômicos severos em repolho, Brassica oleracea variedade capitata L. (Brassicaceae), na área de horticultura localizada na Província de Santa Fé, Argentina. O uso excessivo de inseticidas de largo espectro afeta a ação dos inimigos naturais de P. xylostella em repolho. O presente trabalho teve como objetivo identificar os parasitóides de P. xylostella e determinar sua influência na mortalidade de larvas e pupas em Santa Fé, Argentina. Coletas semanais de larvas e pupas em culturas de repolho foram realizadas aleatoriamente na área durante a primavera de 2006 e 2007, registradas pelo estágio de desenvolvimento: L1 e L2 (Ls = larvas pequenas), (Lm = larvas médias) L3, L4 (Ll = larvas grandes) pré-pupa e pupa (P). Cada indivíduo foi observado diariamente no laboratório até a emergência dos adultos da praga ou dos parasitóides. Foram identificadas as espécies de parasitóides o número de instars e a porcentagem de mortalidade. Os parasitóides registrados foram: Diadegma insulare (Cresson, 1875) (Hymenoptera, Ichneumonidae), Oomyzus sokolowskii (Kurdjumov, 1912) (Hymenoptera, Eulophidae), Cotesia plutellae (Kurdjumov, 1912) (Hymenoptera, Braconidae) e uma espécie não identificada pertencente à família Chalcididae. Além dos parasitóides, um fungo entomopatogênico não identificado foi registrado. Em 2006, os parasitóides mais bem sucedidos foram D. insulare e O. Sokolowskii; enquanto que em 2007 apenas D. insulare exerceu um controle satisfatório, atacando os instares iniciais da praga.

Assessing the risk of pesticide environmental impact in several Argentinian cropping systems with a fuzzy expert indicator.

BACKGROUND: The introduction of transgenic soybean (Glycine max, L.) varieties resistant to glyphosate (GR soybeans) has rapidly expanded in Argentina, increasing pesticide use where only grasslands were previously cultivated. The authors compared an estimate of environmental risk for different crops and active ingredients using the IPEST index, which is based on a fuzzy-logic expert system. For IPEST calculations, four modules are defined, one reflecting the rate of application, the other three reflecting the risk for groundwater, surface water and air. The input variables are pesticide properties, site-specific conditions and characteristics of the pesticide application. The expert system calculates the value of modules according to the degree of membership of the input variables to the fuzzy subsets F (favourable) and U (unfavourable), and they can be aggregated following sets of decision rules. IPEST integrated values of >or= 7 reflect low environmental risk, and values of < 7 reflect high risk. RESULTS: Alfalfa, soybean and wheat showed IPEST values over 7 (low risk), while maize had the lowest IPEST values (high risk). Comparing active ingredients applied in annual and perennial crops, atrazine and acetochlor gave the highest risks of environmental contamination, and they are mainly used in maize. Groundwater was the most affected compartment. CONCLUSIONS: Fuzzy logic provided an easy tool combining different environmental components with pesticide properties to give a simple and accessible risk assessment. These findings provide information about active ingredients that should be replaced in order to protect water and air from pesticide contamination.

Effect of wild flowers on oviposition of Hippodamia variegata (Coleoptera: Coccinellidae) in the laboratory.

Marginal vegetation in crops is very important for natural enemies and their pest control capacity. The effects of Brassica nigra L. (Brassicaceae), Daucus carota L. (Apiaceae), and Sonchus oleraceous L. (Asteraceae) flowers as supplemental food on the number of eggs laid during 7 d and on the preoviposition time in Hippodamia variegata (Goeze, 1777) were studied in the laboratory under conditions of several densities of Acyrthosiphon pisum (Harris, 1776). The results show the presence of flowers of Brassica and Sonchus increased egg production 1.44X and doubled the pre-oviposition period (2.13X). This suggests that the availability of flowers of Brassica and Sonchus as supplemental foods (pollen and nectar) in the marginal vegetation of crops can serve to improve reproductive performance of H. variegata, specifically under conditions of prey limitation. Thus, the increase in fitness of this predator allows a better response to changes in pest density.