Application of proteins from different meat processing wastewater streams as a dietary protein source in animal feed.

The present study aimed to investigate the protein quality indices of meat processing wastewaters protein hydrolysate (FWPH) obtained by Pediococcus lolii fermentation using a rat bioassay. Different diets namely, SD (100% casein), F50 (50% FWPH+50% casein), F75 (75% FWPH+25% casein), and F100 (100% FWPH) were formulated and fed to 28 days old albino male Wistar rats (6 rats each group). SD and F50 group rats showed higher feed and protein intake among all diet group rats (P < 0.05). However, the highest body weight gain (142.16 ± 2.84 g) was noticed in the rats fed with the F50 diet (P < 0.05). F50 group rats presented better feed conversion ratio (FCR), feed efficiency ratio (FER), and protein efficiency ratio (PER) values as compared to other diet group counterparts (P < 0.05). Furthermore, no differences in values of urine, haematological, and serum biochemical parameters were observed in SD and F50 group rats (P > 0.05). However, haematological and serum biochemical parameters were slightly elevated in F100 group rats. F50 group rats presented the higher superoxide dismutase (SOD) and catalase (CAT) activities among all the diet groups (P < 0.05). Relative organ weights, serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT), and alkaline phosphatase (ALP) activities of F50 group rats were found similar to SD rats. Histopathological examination revealed that no incidence of organ toxicity is attributed to the F50 diet. Overall, F50 group rats exhibited good growth and development with no adverse effects in biochemical, haematological, and histopathology parameters. Therefore, FWPH at the 50% level could be used as a potential dietary protein source in feed for better growth performance of the animal.

Recovery, physicochemical and functional characteristics of proteins from different meat processing wastewater streams.

Recovery, physicochemical and functional characteristics of proteins recovered from different meat processing wastewater streams were revealed in the present study. Wastewaters from surimi processing (SPW) and slaughterhouses, namely fish (FSW), cattle (CSW), poultry (PSW), and goat (GSW), exhibited protein, fat, ash, moisture, and microbial load in the range of 1.28-7.04%, 0.86-2.34%, 0.02-0.80%, 89.81-97.44%, and 5.33-5.81 CFU/mL, respectively. Among the wastewaters, SPW presented slightly higher protein (7.04%), fat (2.34%), and ash (0.80%) contents (P < 0.05). Furthermore, proteins recovered from SPW (SPWP) and FSW (FSWP), CSW (CSWP), PSW (PSWP), and GSW (GSWP) presented yield, protein, fat, ash, and moisture content in the range of 55.54-76.81%, 65.86-78.22%, 7.26-11.45%, 4.58-11.75%, and 5.67-14.79%. All protein samples displayed higher essential amino acid (EAA) content with leucine (8.47-14.52 g/100 g) as a predominant amino acid. GSWP and SPWP scored the highest and lowest EAA contents, respectively. SPWP displayed myofibrillar proteins as dominant proteins, while slaughterhouses' wastewater proteins showed blood proteins as major proteins. ß-Sheet is the major secondary structure presented by all protein samples. SPWP showed the highest lightness value as compared to other protein counterparts (P < 0.05). All protein samples from slaughterhouse wastewaters had the lowest protein solubility at pH 4.5. However, SPWP presented minimum solubility at pH 5.5. Among all protein samples, SPWP presented slightly higher water holding capacity and foaming property (P < 0.05), whereas FSWP displayed slightly higher emulsion property (P < 0.05). Overall, all meat processing wastewater streams served as good sources of high-quality proteins, which could be used as protein ingredients in animal feed formulation.