Central pattern generator network model for the alternating hind limb gait of rats based on the modified Van der Pol equation.

Herein, we employed a central pattern generator (CPG), a spinal cord neural network that regulates lower-limb gait during intra-spinal micro-stimulation (ISMS). Particularly, ISMS was used to determine the spatial distribution pattern of CPG sites in the spinal cord and the signal regulation pattern that induced the CPG network to produce coordinated actions. Based on the oscillation phenomenon of the single CPG neurons of Van der Pol (VDP) oscillators, a double-cell CPG neural network model was constructed to realise double lower limbs, six-joint modelling, the simulation of 12 neural circuits, the CPG loci characterising stimuli-inducing alternating movements and changes in polarity stimulation signals in rat hindlimbs, and leg-state change movements. The feasibility and effectiveness of the CPG neural network were verified by recording the electromyographic burst-release mode of the flexor and extensor muscles of the knee joints during CPG electrical stimulation. The results revealed that the output pattern of the CPG presented stable rhythm and coordination characteristics. The 12-neuron CPG model based on the improved VDP equation realised single-point control while significantly reducing the number of stimulation electrodes in the gait training of spinal cord injury patients. We believe that this study advances motor function recovery in rehabilitation medicine.

Chopstick-Like Structure for the Free Transfer of Microdroplets in Robot Chemistry Laboratory.

As we all know, chopsticks can hold food, so can we use this method to carry Newtonian fluids such as droplets? This paper studies the process of this transfer and uses this method to realize the manipulation of open microfluidics by robots. To realize this transfer operation, we first analyzed the force of droplets in this chopstick-like structure and found that the bidirectional movement of droplets in this structure can be achieved by changing the structural parameters. Afterward, the whole process of the transfer of droplets using the chopstick-like structure was analyzed, and the parameter requirements for realizing this transfer were determined. The research in this paper provides a theoretical basis for the controllable manipulation of droplets which can be widely used in unmanned laboratories.

Droplet Tweezers Based on the Hydrophilic-Hydrophobic Interface Structure and Their Biological Application.

Droplet controllable operation has wide applications in microfluidics, biomedicine, microreactors, and other fields. Droplets can spontaneously transfer from a high-energy state to a low-energy state, but how to reverse transfer the droplets is a difficult task. In this article, we use a special hydrophilic-hydrophobic interphase structure (HHIS) to achieve this reverse transfer. We specifically study the critical conditions under which droplet transfer can be achieved. The length of the hydrophilic surface in this structure and the hydrophilic/hydrophobic properties of the surface must be in the appropriate range. Based on this, an optimized structure used to transfer droplets was designed. Finally, we carried out research on biological applications and successfully achieved the transfer of droplets from zebrafish eggs and zebrafish larvae. This unique method is low-cost, biofriendly, and highly applicable to various surfaces, illustrating the great potential in chemical and biological analysis.

Influence of liquid bridge formation process on its stability in nonparallel plates.

The formation of a liquid bridge in non-parallel plates is very common and the stability (whether or not it can move spontaneously) of such liquid bridges has been studied a lot for industry, e.g. in printing applications. It is generally considered that the liquid bridge stability is determined by Contact Angle (CA), Contact Angle Hysteresis (CAH), the position of the liquid bridge (represented as P) and the dihedral angle (θ) between non-parallel plates. The stability equation is θ = f(CA, CAH, P). Since P is a process quantity, which is difficult to determine, so it is also difficult to obtain the critical equation for the stability of the liquid bridge. In the previous study (J. Colloid Interface Sci., 2017, 492, 207-217), based on the fitting simulation results, the critical equation about CA, CAH and θ is obtained, as θ = f(CA, CAH). However, in some special cases, the results are still biased (e.g. the weak hydrophilic situation). In this paper, unlike simulation, we get the critical equation θ = f(CA, CAH) from a theoretical point of view. For the first time, by in-depth analysis of the process of liquid bridge formation, the theoretical calculation equation of P is obtained as P = f(CA, CAH, θ). And then, combining the equations θ = f(CA, CAH, P) and P = f(CA, CAH, θ), the theoretical equation is obtained. A lot of simulations and experiments were performed to verify our theoretical equation. Furthermore, comparing our equation with the previous equation, it was found that our equation is more consistent with the experimental results (error less than 0.2°). Finally, the importance of considering the liquid bridging process (the function of P) for stability analysis is illustrated by comparing the results with those not considered (the difference is more than 20% in some cases). The outputs of this paper provide in-depth theoretical support for the analysis and application of liquid bridges.

Motion of droplets into hydrophobic parallel plates.

Due to the superior operability and good anti-interference, the prospect of controlling microdroplets using a parallel plate structure (PPS) is very promising. However, in practical applications, droplets in such structures are often affected by various factors, resulting in deformation, evaporation, stress rupture and other phenomena, leading to equipment failure. Therefore, how to simply and effectively transfer liquid droplets to PPS to maintain the stable and efficient operation of the system has become an urgent problem to be solved. In this paper, a simple and effective ratchet-like strategy (relaxing and squeezing actions) is introduced to transfer droplets. To analyze the mechanism of the strategy and optimize the control, we conduct this study from three aspects. First, the droplet movement trend is obtained by analyzing the pressure between SPS and PPS. Second, the reasons why the droplet can achieve this inward motion are investigated. Through theoretical analysis, which is also proven by simulations and experiments, we creatively put forward that the asymmetric change of the contact angle (CA) induced by the asymmetric structure is the fundamental cause of this kind of motion. Due to the asymmetric change of the contact angle, the CA in the PPS will reach the advancing angle first in the squeezing process, and the CA in the SPS will reach the receding angle first in the relaxing process, thus causing the inward movement of the droplet. Third, to optimize this strategy, the effects of the following governing parameters are researched individually based on the corresponding simulations and experiments: the control parameters (the initial gap width of the PPS H 0 and the amount of squeezing and relaxing of ΔH) and the thickness of the top plate. Subsequently, an optimized ratchet-like cycle is achieved. In summary, these findings not only provide a new method by which to realize the movement of droplets toward hydrophobic PPSs but also creatively point out the cause of the ratchet strategy, which can be applied in many microfluidics fields.