Epigenetic control during root development and symbiosis.

The roots of plants play multiples functions that are essential for growth and development, including anchoring to the soil and water and nutrient acquisition. These underground organs exhibit the plasticity to modify their root system architecture in response to environmental cues allowing adaptation to change in water and nutrient availability. In addition, roots enter in mutualistic interactions with soil microorganisms, e.g. the root nodule symbiosis established between a limited group of plants and nitrogen fixing soil bacteria and the arbuscular mycorrhiza symbiosis involving most land plants and fungi of the Glomeromycetes phylum. In the past 20 years, genetic approaches allowed the identification and functional characterization of genes required for the specific programs of root development, root nodule and arbuscular mycorrhiza symbioses. These genetic studies provided evidence that the program of root nodule symbiosis recruited components of the arbuscular mycorrhiza symbiosis and the root developmental programs. The execution of these programs is strongly influenced by epigenetic changes -DNA methylation and histone post-translational modifications- that alter chromatin conformation modifying the expression of key genes. In this review, we summarize recent advances that highlighted how DNA methylation and histone post-translational modifications, as well as chromatin remodeling factors and long non-coding RNAs, shape the root system architecture and allow the successful establishment of both root nodule and arbuscular mycorrhiza symbioses. We anticipate that the analysis of dynamic epigenetic changes and chromatin 3D structure in specific single-cells or tissue types of root organs will illuminate our understanding of how root developmental and symbiotic programs are orchestrated, opening exciting questions and new perspectives to modulate agronomical and ecological traits linked to nutrient acquisition.

A lateral organ boundaries domain transcription factor acts downstream of the auxin response factor 2 to control nodulation and root architecture in Medicago truncatula.

Legume plants develop two types of root postembryonic organs, lateral roots and symbiotic nodules, using shared regulatory components. The module composed by the microRNA390, the Trans-Acting SIRNA3 (TAS3) RNA and the Auxin Response Factors (ARF)2, ARF3, and ARF4 (miR390/TAS3/ARFs) mediates the control of both lateral roots and symbiotic nodules in legumes. Here, a transcriptomic approach identified a member of the Lateral Organ Boundaries Domain (LBD) family of transcription factors in Medicago truncatula, designated MtLBD17/29a, which is regulated by the miR390/TAS3/ARFs module. ChIP-PCR experiments evidenced that MtARF2 binds to an Auxin Response Element present in the MtLBD17/29a promoter. MtLBD17/29a is expressed in root meristems, lateral root primordia, and noninfected cells of symbiotic nodules. Knockdown of MtLBD17/29a reduced the length of primary and lateral roots and enhanced lateral root formation, whereas overexpression of MtLBD17/29a produced the opposite phenotype. Interestingly, both knockdown and overexpression of MtLBD17/29a reduced nodule number and infection events and impaired the induction of the symbiotic genes Nodulation Signaling Pathway (NSP) 1 and 2. Our results demonstrate that MtLBD17/29a is regulated by the miR390/TAS3/ARFs module and a direct target of MtARF2, revealing a new lateral root regulatory hub recruited by legumes to act in the root nodule symbiotic program.

Características químicas e salino-sodicidade dos solos do Perímetro Irrigado Califórnia, SE, Brasil / Characteristcs chemical and soil salinization in the Irrigated District of California, SE, Brazil

O manejo inadequado da irrigação pode resultar no acúmulo de sais no solo, cuja intensidade depende da qualidade da água utilizada na irrigação, da fração de lixiviação adotada e da demanda evapotranspirativa da região. O presente trabalho teve como objetivo avaliar os atributos físicos e químicos dos solos do perímetro irrigado Califórnia, localizado no extremo noroeste do Estado de Sergipe. Para tal, foram realizadas amostragens em 42 lotes, correspondendo a 16,4 por cento dos lotes deste perímetro. As maiores concentrações de sais e de sódio ocorreram na camada superficial (0-0,2m) do solo, o que evidenciou a falta de um manejo da irrigação adequado visando ao controle da salinidade. O aumento do pH do solo foi correlacionado significativamente com a porcentagem de sódio trocável, atingindo valores acima de 9,5. Correlações positivas significativas foram observadas entre a condutividade elétrica e os teores de Ca e Mg, indicando que sais desses íons podem estar se acumulando no solo pela ascensão do lençol freático, associado à ausência de lixiviação e drenagem.

The improper irrigation management may result in the accumulation of salts in the soil profile, which intensity depends on the irrigation water quality, the leaching fraction adopted and on the evapotranspiration demand of the region. The objective of the present study was to study the physical and chemical characteristics of the soils in the irrigated district of California, located in the northwest of the State of Sergipe. Thus, soil sampling were made in 54 lots, corresponding to 16.4 percent of the lots of this district. The highest salt and sodium concentrations occurred in the shallowest soil layer (0-0.2m), evidencing the lack of an appropriate irrigation management in order to control the salinity build up. The increasing of the soil pH was significantly correlated to the exchangeable sodium percentage, reaching values over 9.5. Significant positive correlations were observed between the electrical conductivity and the Ca and Mg content, indicating that salts of both ions could be accumulating in soil by the capillary rise of the water table, associated to the lack of leaching and drainage.

Emissão foliar, relações iônicas e produção do coqueiro irrigado com água salina / Leaf emission, ionic relations and production of coconut irrigated with saline water

A necessidade de uso de águas salinas na agricultura é crescente, devido à pressão sobre águas de boa qualidade, principalmente para consumo humano. Com este objetivo, foram estudados os efeitos de quatro níveis de condutividade elétrica da água de irrigação (CEa: 0,1; 5,0; 10,0 e 15,0dS m-1), obtidos mediante adição de NaCl sobre o desenvolvimento do coqueiro (Cocos nucifera L.) cv. "Anão Verde", com 3,5 anos de idade. O experimento foi conduzido em blocos casualizados, em solo arenoso, irrigado por microaspersão, no município de Parnamirim-RN, no período de janeiro de 2000 a março de 2001. A salinidade da água aumentou os intervalos de emissão foliar e de inflorescência e o número de flores femininas, bem como os teores de Na e de Cl na folha número 14, acompanhado de relações antagônicas entre Na-Ca, Na-K e Cl-N e sinergismo entre Na-Mg, mas sem manifestar sintomas visuais de efeitos tóxicos. Os teores ótimos de Na e Cl na folha 14 foram, respectivamente, 0,207 e 0,580 por cento.

The necessity to use saline waters in agriculture has increased due to pressure on good quality water, principally, for human consumption. With this objective, the effects of four levels of electrical conductivity (0.1, 5.0, 10.0 and 15.0dS m-1) of irrigation water obtained by addition of NaCl were studied on the development of 3.5 years old coconut (Cocos nucifera L.) cv. 'Anão Verde'. The experiment was conducted in a sandy soil adopting a completely randomized block design and micro sprinkler irrigation system in the municipality of Parnamirim - RN, Brazil, during the period of January, 2000 to March, 2001. The salinity of water increased the interval of leaf emission and inflorescences and the number of female flowers per inflorescence as well as the contents of Na and Cl in leaf 14, accompanied by antagonism between Na-Ca, Na-K and Cl-N, and synergism between Na-Mg but without any visual symptoms of toxic effects. The optimum levels of Na and Cl in 14th leaf were found to be 0.207 and 0.580 percent.