Quantitative analysis of magnetic resonance images for characterization of blood-brain barrier dysfunction in dogs with brain tumors.

BACKGROUND: Blood-brain barrier (BBB) permeability can be assessed quantitatively using advanced imaging analysis. HYPOTHESIS/OBJECTIVES: Quantification and characterization of blood-brain barrier dysfunction (BBBD) patterns in dogs with brain tumors can provide useful information about tumor biology and assist in distinguishing between gliomas and meningiomas. ANIMALS: Seventy-eight hospitalized dogs with brain tumors and 12 control dogs without brain tumors. METHODS: In a 2-arm study, images from a prospective dynamic contrast-enhanced (DCE; n = 15) and a retrospective archived magnetic resonance imaging study (n = 63) were analyzed by DCE and subtraction enhancement analysis (SEA) to quantify BBB permeability in affected dogs relative to control dogs (n = 6 in each arm). For the SEA method, 2 ranges of postcontrast intensity differences, that is, high (HR) and low (LR), were evaluated as possible representations of 2 classes of BBB leakage. BBB score was calculated for each dog and was associated with clinical characteristics and tumor location and class. Permeability maps were generated, using the slope values (DCE) or intensity difference (SEA) of each voxel, and analyzed. RESULTS: Distinctive patterns and distributions of BBBD were identified for intra- and extra-axial tumors. At a cutoff of 0.1, LR/HR BBB score ratio yielded a sensitivity of 80% and specificity of 100% in differentiating gliomas from meningiomas. CONCLUSIONS AND CLINICAL IMPORTANCE: Blood-brain barrier dysfunction quantification using advanced imaging analyses has the potential to be used for assessment of brain tumor characteristics and behavior and, particularly, to help differentiating gliomas from meningiomas.

Activity of native and commercial strains of Metarhizium spp. against the poultry red mite Dermanyssus gallinae under different environmental conditions.

The poultry red mite (PRM), Dermanyssus gallinae, is a major pest of laying hens with extremely limited control means. To evaluate the potential of natural and commercial entomopathogenic fungi (EPF) for use against D. gallinae, we tested four wild populations of D. gallinae from Israeli farms. The genus Aspergillus was identified as the most abundant isolates from the mites. Additionally, eight new isolates of Metarhizium belonging to the specie M. brunneum were identified. At all sites from which mites were collected in both seasons, the abundance of fungi on D. gallinae was greater during the winter season than during the summer season. Through indirect inoculations of adult D. gallinae, we examined the virulence of the native Metarhizium species, the commercial strain Ma-43 and a previously described acaropathogenic strain (Ma-7). All of the Metarhizium strains caused 56-95% mortality of adult mites by seven days after inoculation at a concentration of 5 × 105 conidia per cm2. The efficacies of Ma-43, Ma-7 and the most promising native strain were tested under optimal abiotic conditions (28°C; 85-100% RH) and abiotic conditions similar to those typically found in a poultry house (30 °C; 60% RH). Under optimal conditions, the efficacy of all three stains ranged between 85 and 92%. In contrast, under poultry-house conditions, the efficacy of control ranged between 30 and 40%. The incidence of mycoses on mite cadavers was significantly decreased under poultry-house conditions. These results demonstrate the potential of native and commercial Metarhizium strains for use as biopesticides. Future research should address suitable delivery methods and formulations for the effective control of D. gallinae under poultry-house conditions.